Abstract:
:Bacterial symbionts of insects have been proposed for blocking transmission of vector-borne pathogens. However, in many vector models the ecology of symbionts and their capability of cross-colonizing different hosts, an important feature in the symbiotic control approach, is poorly known. Here we show that the acetic acid bacterium Asaia, previously found in the malaria mosquito vector Anopheles stephensi, is also present in, and capable of cross-colonizing other sugar-feeding insects of phylogenetically distant genera and orders. PCR, real-time PCR and in situ hybridization experiments showed Asaia in the body of the mosquito Aedes aegypti and the leafhopper Scaphoideus titanus, vectors of human viruses and a grapevine phytoplasma respectively. Cross-colonization patterns of the body of Ae. aegypti, An. stephensi and S. titanus have been documented with Asaia strains isolated from An. stephensi or Ae. aegypti, and labelled with plasmid- or chromosome-encoded fluorescent proteins (Gfp and DsRed respectively). Fluorescence and confocal microscopy showed that Asaia, administered with the sugar meal, efficiently colonized guts, male and female reproductive systems and the salivary glands. The ability in cross-colonizing insects of phylogenetically distant orders indicated that Asaia adopts body invasion mechanisms independent from host-specific biological characteristics. This versatility is an important property for the development of symbiont-based control of different vector-borne diseases.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Crotti E,Damiani C,Pajoro M,Gonella E,Rizzi A,Ricci I,Negri I,Scuppa P,Rossi P,Ballarini P,Raddadi N,Marzorati M,Sacchi L,Clementi E,Genchi M,Mandrioli M,Bandi C,Favia G,Alma A,Daffonchio Ddoi
10.1111/j.1462-2920.2009.02048.xsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
3252-64issue
12eissn
1462-2912issn
1462-2920pii
EMI2048journal_volume
11pub_type
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