Abstract:
:Most of the oil in low temperature, non-uplifted reservoirs is biodegraded due to millions of years of microbial activity, including via methanogenesis from crude oil. To evaluate stimulating additional methanogenesis in already heavily biodegraded oil reservoirs, oil sands samples were amended with nutrients and electron acceptors, but oil sands bitumen was the only organic substrate. Methane production was monitored for over 3000 days. Methanogenesis was observed in duplicate microcosms that were unamended, amended with sulfate or that were initially oxic, however methanogenesis was not observed in nitrate-amended controls. The highest rate of methane production was 0.15 μmol CH4 g-1 oil d-1 , orders of magnitude lower than other reports of methanogenesis from lighter crude oils. Methanogenic Archaea and several potential syntrophic bacterial partners were detected following the incubations. GC-MS and FTICR-MS revealed no significant bitumen alteration for any specific compound or compound class, suggesting that the very slow methanogenesis observed was coupled to bitumen biodegradation in an unspecific manner. After 3000 days, methanogenic communities were amended with benzoate resulting in methanogenesis rates that were 110-fold greater. This suggests that oil-to-methane conversion is limited by the recalcitrant nature of oil sands bitumen, not the microbial communities resident in heavy oil reservoirs.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
de Rezende JR,Oldenburg TBP,Korin T,Richardson WDL,Fustic M,Aitken CM,Bowler BFJ,Sherry A,Grigoryan A,Voordouw G,Larter SR,Head IM,Hubert CRJdoi
10.1111/1462-2920.14995subject
Has Abstractpub_date
2020-08-01 00:00:00pages
3049-3065issue
8eissn
1462-2912issn
1462-2920journal_volume
22pub_type
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