Anthropogenic effects on bacterial diversity and function along a river-to-estuary gradient in Northwest Greece revealed by metagenomics.

Abstract:

:Studies assessing the effects of anthropogenic inputs on the taxonomic and functional diversity of bacterioplankton communities in lotic ecosystems are limited. Here, we applied 16S rRNA gene amplicon and whole-genome shotgun sequencing to examine the microbial diversity in samples from the Kalamas River (Northwest Greece), a mid-size river that runs through agricultural and NATURA-protected areas, but also receives urban sewage from a large city through a manmade ditch. Samples from three different locations between the exit of the ditch and the estuary, during three different months showed that temporal differences of taxonomic and functional diversity were more pronounced than spatial ones, and <1% of total taxa were shared among all samples, revealing a highly dynamic ecosystem. Comparisons of gene diversity with other aquatic habitats showed that only the high flow winter samples resembled more to freshwater environments while samples during the decreased water flow months were dominated by sewage inputs and soil-related organisms. Notably, microbial human gut signals were detectable over background freshwater and soil/runoff related signals, even at tens of kilometers downstream the city. These findings revealed the significance of allochthonous inputs on the composition and dynamics of river bacterial communities, and highlighted the potential of metagenomics for source tracking purposes.

journal_name

Environ Microbiol

authors

Meziti A,Tsementzi D,Ar Kormas K,Karayanni H,Konstantinidis KT

doi

10.1111/1462-2920.13303

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

4640-4652

issue

12

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

杂志文章
  • Hypolithic microbial communities: between a rock and a hard place.

    abstract::Drylands are the largest terrestrial biome on Earth and a ubiquitous feature is desert pavement terrain, comprising rocks embedded in the mineral soil surface. Quartz and other translucent rocks are common and microbial communities termed hypoliths develop as biofilms on their ventral surfaces. In extreme deserts thes...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2012.02821.x

    authors: Chan Y,Lacap DC,Lau MC,Ha KY,Warren-Rhodes KA,Cockell CS,Cowan DA,McKay CP,Pointing SB

    更新日期:2012-09-01 00:00:00

  • Evidence of distinct pathways for bacterial degradation of the steroid compound cholate suggests the potential for metabolic interactions by interspecies cross-feeding.

    abstract::The distribution and the metabolic pathways of bacteria degrading steroid compounds released by eukaryotic organisms were investigated using the bile salt cholate as model substrate. Cholate-degrading bacteria could be readily isolated from freshwater environments. All isolated strains transiently released steroid deg...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12407

    authors: Holert J,Yücel O,Suvekbala V,Kulić Z,Möller H,Philipp B

    更新日期:2014-05-01 00:00:00

  • Distribution of aerobic anoxygenic phototrophs in temperate freshwater systems.

    abstract::The presence of aerobic anoxygenic phototrophs (AAPs) was recently reported from various marine environments; however, there is little information regarding their distribution in fresh waters. We surveyed a number of freshwater systems in central Europe, by infra-red fluorometry, infra-red epifluorescence microscopy, ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01615.x

    authors: Masín M,Nedoma J,Pechar L,Koblízek M

    更新日期:2008-08-01 00:00:00

  • Unusual deprivation of compatible solutes in Acinetobacter baumannii.

    abstract::The opportunistic human pathogen Acinetobacter baumannii is one of the leading causes of nosocomial infections. The high prevalence of multidrug-resistant strains, a high adaptability to changing environments and an overall pronounced stress resistance contribute to persistence and spread of the bacteria in hospitals ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14951

    authors: Zeidler S,Müller V

    更新日期:2020-04-01 00:00:00

  • Does quorum sensing interference affect the fitness of bacterial pathogens in the real world?

    abstract::Many bacterial pathogens rely on quorum sensing to control virulence gene expression. Based on numerous experiments conducted under well-defined conditions, quorum sensing interference is considered as a promising strategy to tackle infections and thus might have the potential to (partially) replace antibiotics. Despi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14446

    authors: Liu Y,Qin Q,Defoirdt T

    更新日期:2018-11-01 00:00:00

  • Metabolic response of Agrobacterium tumefaciens 5A to arsenite.

    abstract::Wide-spread abundance in soil and water, coupled with high toxicity have put arsenic at the top of the list of environmental contaminants. Early studies demonstrated that both concentration and the valence state of inorganic arsenic (arsenite, As(III) vs. arsenate As(V)) can be modulated by microbes. Using genetics, t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13615

    authors: Tokmina-Lukaszewska M,Shi Z,Tripet B,McDermott TR,Copié V,Bothner B,Wang G

    更新日期:2017-02-01 00:00:00

  • Bacterial community shifts in taxa and diversity in response to localized organic loading in the deep sea.

    abstract::The deep sea is a unique and extreme environment characterized by low concentrations of highly recalcitrant carbon. As a consequence, large organic inputs have potential to cause significant perturbation. To assess the impact of organic enrichment on deep sea microbial communities, we investigated bacterial diversity ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02072.x

    authors: Goffredi SK,Orphan VJ

    更新日期:2010-02-01 00:00:00

  • In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.

    abstract::Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-canonical amino acid...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12436

    authors: Hatzenpichler R,Scheller S,Tavormina PL,Babin BM,Tirrell DA,Orphan VJ

    更新日期:2014-08-01 00:00:00

  • Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods.

    abstract::The phylogenetic diversity of Bacteria and Archaea within a biodegraded, mesothermic petroleum reservoir in the Schrader Bluff Formation of Alaska was examined by two culture-independent methods based on fosmid and small-subunit rRNA gene PCR clone libraries. Despite the exclusion of certain groups by each method, the...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01751.x

    authors: Pham VD,Hnatow LL,Zhang S,Fallon RD,Jackson SC,Tomb JF,DeLong EF,Keeler SJ

    更新日期:2009-01-01 00:00:00

  • Off-axis symbiosis found: Characterization and biogeography of bacterial symbionts of Bathymodiolus mussels from Lost City hydrothermal vents.

    abstract::Organisms at hydrothermal vents inhabit discontinuous chemical 'islands' along mid-ocean ridges, a scenario that may promote genetic divergence among populations. The 2003 discovery of mussels at the Lost City Hydrothermal Field provided a means of evaluating factors that govern the biogeography of symbiotic bacteria ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.01113.x

    authors: DeChaine EG,Bates AE,Shank TM,Cavanaugh CM

    更新日期:2006-11-01 00:00:00

  • Novel acid resistance genes from the metagenome of the Tinto River, an extremely acidic environment.

    abstract::Microorganisms that thrive in acidic environments are endowed with specialized molecular mechanisms to survive under this extremely harsh condition. In this work, we performed functional screening of six metagenomic libraries from planktonic and rhizosphere microbial communities of the Tinto River, an extremely acidic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12021

    authors: Guazzaroni ME,Morgante V,Mirete S,González-Pastor JE

    更新日期:2013-04-01 00:00:00

  • Quorum sensing regulates 'swim-or-stick' lifestyle in the phycosphere.

    abstract::Interactions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15228

    authors: Fei C,Ochsenkühn MA,Shibl AA,Isaac A,Wang C,Amin SA

    更新日期:2020-11-01 00:00:00

  • Towards a unified data infrastructure to support European and global microbiome research: a call to action.

    abstract::High-quality microbiome research relies on the integrity, management and quality of supporting data. Currently biobanks and culture collections have different formats and approaches to data management. This necessitates a standard data format to underpin research, particularly in line with the FAIR data standards of f...

    journal_title:Environmental microbiology

    pub_type: 信件

    doi:10.1111/1462-2920.15323

    authors: Ryan MJ,Schloter M,Berg G,Kinkel LL,Eversole K,Macklin JA,Rybakova D,Sessitsch A

    更新日期:2021-01-01 00:00:00

  • Ammonia-oxidizing Crenarchaeota and nitrification inside the tissue of a colonial ascidian.

    abstract::Marine Crenarchaeota represent an abundant component of the oceanic microbiota that play an important role in the global nitrogen cycle. Here we report the association of the colonial ascidian Cystodytes dellechiajei with putative ammonia-oxidizing Crenarchaeota that could actively be involved in nitrification inside ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01761.x

    authors: Martínez-García M,Stief P,Díaz-Valdés M,Wanner G,Ramos-Esplá A,Dubilier N,Antón J

    更新日期:2008-11-01 00:00:00

  • Control and host-dependent activation of insect toxin expression in a root-associated biocontrol pseudomonad.

    abstract::Pseudomonas fluorescens CHA0 is a root-associated biocontrol agent that suppresses soil-borne fungal diseases of crops. Remarkably, the pseudomonad is also endowed with systemic and oral activity against pest insects which depends on the production of the insecticidal Fit toxin. The toxin gene (fitD) is part of a viru...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12050

    authors: Péchy-Tarr M,Borel N,Kupferschmied P,Turner V,Binggeli O,Radovanovic D,Maurhofer M,Keel C

    更新日期:2013-03-01 00:00:00

  • Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing.

    abstract::It is well recognized that a dynamic biofilm develops upon plant biomass in the herbivore gastrointestinal tract, but this component of the microbiome has not previously been specifically sampled, or directly compared with the biodiversity present in the planktonic fraction of digesta. In this study, the digesta colle...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00721.x

    authors: Larue R,Yu Z,Parisi VA,Egan AR,Morrison M

    更新日期:2005-04-01 00:00:00

  • Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment.

    abstract::Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14297

    authors: Müller AL,Pelikan C,de Rezende JR,Wasmund K,Putz M,Glombitza C,Kjeldsen KU,Jørgensen BB,Loy A

    更新日期:2018-08-01 00:00:00

  • The cyclin dependent kinase subunit Cks1 is required for infection-associated development of the rice blast fungus Magnaporthe oryzae.

    abstract::Cell cycle regulation is pivotal for proper cell division and cellular differentiation in eukaryotic cells. The central regulators that govern eukaryotic cell cycle progression are cyclin-dependent kinases (CDKs) and their partners. Here, we report that Magnaporthe oryzae CKS1 encodes a cyclin-dependent kinase subunit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13796

    authors: Yue X,Que Y,Deng S,Xu L,Oses-Ruiz M,Talbot NJ,Peng Y,Wang Z

    更新日期:2017-10-01 00:00:00

  • Molecular evidence for widespread occurrence of Foraminifera in soils.

    abstract::Environmental SSU rDNA-based surveys are contributing to the dramatic revision of eukaryotic high-level diversity and phylogeny as the number of sequence data increases. This ongoing revolution gives the opportunity to test for the presence of some eukaryotic taxa in environments where they have not been found using c...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02225.x

    authors: Lejzerowicz F,Pawlowski J,Fraissinet-Tachet L,Marmeisse R

    更新日期:2010-09-01 00:00:00

  • Microbial players involved in the decline of filamentous and colonial cyanobacterial blooms with a focus on fungal parasitism.

    abstract::In the forthcoming decades, it is widely believed that the dominance of colonial and filamentous bloom-forming cyanobacteria (e.g. Microcystis, Planktothrix, Anabaena and Cylindrospermopsis) will increase in freshwater systems as a combined result of anthropogenic nutrient input into freshwater bodies and climate chan...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.12860

    authors: Gerphagnon M,Macarthur DJ,Latour D,Gachon CM,Van Ogtrop F,Gleason FH,Sime-Ngando T

    更新日期:2015-08-01 00:00:00

  • Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans.

    abstract::In diseased piglets from two Dutch pig-breeding farms with neonatal diarrhoea for more than a year, culture and PCR analyses identified the involved microorganism as Clostridium difficile PCR ribotype 078 harbouring toxin A (tcdA) and B (tcdB), and binary toxin genes. Isolated strains showed a 39 bp deletion in the tc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01790.x

    authors: Debast SB,van Leengoed LA,Goorhuis A,Harmanus C,Kuijper EJ,Bergwerff AA

    更新日期:2009-02-01 00:00:00

  • Disruption of photoautotrophic intertidal mats by filamentous fungi.

    abstract::Ring-like structures, 2.0-4.8 cm in diameter, observed in photosynthetic microbial mats on the Wadden Sea island Schiermonnikoog (the Netherlands) showed to be the result of the fungus Emericellopsis sp. degrading the photoautotrophic top layer of the mat. The mats were predominantly composed of cyanobacteria and diat...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12835

    authors: Carreira C,Staal M,Falkoski D,de Vries RP,Middelboe M,Brussaard CP

    更新日期:2015-08-01 00:00:00

  • EukRef-Ciliophora: a manually curated, phylogeny-based database of small subunit rRNA gene sequences of ciliates.

    abstract::High-throughput sequencing (HTS) surveys, among the most common approaches currently used in environmental microbiology, require reliable reference databases to be correctly interpreted. The EukRef Initiative (eukref.org) is a community effort to manually screen available small subunit (SSU) rRNA gene sequences and pr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14264

    authors: Boscaro V,Santoferrara LF,Zhang Q,Gentekaki E,Syberg-Olsen MJ,Del Campo J,Keeling PJ

    更新日期:2018-06-01 00:00:00

  • Comparison of sterol and fatty acid profiles of chytrids and their hosts reveals trophic upgrading of nutritionally inadequate phytoplankton by fungal parasites.

    abstract::Chytrids are ubiquitous fungal parasites in aquatic ecosystems, infecting representatives of all major phytoplankton groups. They repack carbon from inedible phytoplankton hosts into easily ingested chytrid propagules (zoospores), rendering this carbon accessible to zooplankton. Grazing on zoospores may circumvent bot...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14489

    authors: Gerphagnon M,Agha R,Martin-Creuzburg D,Bec A,Perriere F,Rad-Menéndez C,Gachon CMM,Wolinska J

    更新日期:2019-03-01 00:00:00

  • Identification and analysis of the bacterial endosymbiont specialized for production of the chemotherapeutic natural product ET-743.

    abstract::Ecteinascidin 743 (ET-743, Yondelis) is a clinically approved chemotherapeutic natural product isolated from the Caribbean mangrove tunicate Ecteinascidia turbinata. Researchers have long suspected that a microorganism may be the true producer of the anticancer drug, but its genome has remained elusive due to our inab...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12908

    authors: Schofield MM,Jain S,Porat D,Dick GJ,Sherman DH

    更新日期:2015-10-01 00:00:00

  • Phylogenetic identification and metabolism of marine dimethylsulfide-consuming bacteria.

    abstract::Microbial consumption is one of the main processes, along with photolysis and ventilation, that remove the biogenic trace gas dimethylsulfide (DMS) from the surface ocean. Although a few isolates of marine bacteria have been studied for their ability to utilize DMS, little is known about the characteristics or phyloge...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01102.x

    authors: Vila-Costa M,Del Valle DA,González JM,Slezak D,Kiene RP,Sánchez O,Simó R

    更新日期:2006-12-01 00:00:00

  • Free Shiga toxin 1-encoding bacteriophages are less prevalent than Shiga toxin 2 phages in extraintestinal environments.

    abstract::Stx bacteriophages are involved in the pathogenicity of Stx-producing Escherichia coli. Induction of the Stx phage lytic cycle increases Stx expression and releases Stx phages that reach extracellular environments. Stx phage family comprises different phages that harbour any stx subtype. Stx2 is closely related with s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13053

    authors: Grau-Leal F,Quirós P,Martínez-Castillo A,Muniesa M

    更新日期:2015-11-01 00:00:00

  • Distribution of specific tetracycline and erythromycin resistance genes in environmental samples assessed by macroarray detection.

    abstract::A macroarray system was developed to screen environmental samples for the presence of specific tetracycline (Tc(R)) and erythromycin (erm(R)) resistance genes. The macroarray was loaded with polymerase chain reaction (PCR) amplicons of 23 Tc(R) genes and 10 erm(R) genes. Total bacterial genomic DNA was extracted from ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01190.x

    authors: Patterson AJ,Colangeli R,Spigaglia P,Scott KP

    更新日期:2007-03-01 00:00:00

  • Detection and activity of insertion sequences in environmental strains of Burkholderia.

    abstract::The presence of two insertion sequences, IS406 and IS407, was tested by polymerase chain reaction (PCR) amplification in 25 strains representing 15 Burkholderia species and the close relative Ralstonia pickettii. A total of 50% of the 25 strains contained at least one of the two insertion sequences (ISs) and a statist...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00251.x

    authors: Miché L,Faure D,Blot M,Cabanne-Giuli E,Balandreau J

    更新日期:2001-12-01 00:00:00

  • Methanogenic toluene metabolism: community structure and intermediates.

    abstract::Toluene is a model compound used to study the anaerobic biotransformation of aromatic hydrocarbons. Reports indicate that toluene is transformed via fumarate addition to form benzylsuccinate or by unknown mechanisms to form hydroxylated intermediates under methanogenic conditions. We investigated the mechanism(s) of s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02631.x

    authors: Fowler SJ,Dong X,Sensen CW,Suflita JM,Gieg LM

    更新日期:2012-03-01 00:00:00