Ammonia-oxidizing Crenarchaeota and nitrification inside the tissue of a colonial ascidian.

Abstract:

:Marine Crenarchaeota represent an abundant component of the oceanic microbiota that play an important role in the global nitrogen cycle. Here we report the association of the colonial ascidian Cystodytes dellechiajei with putative ammonia-oxidizing Crenarchaeota that could actively be involved in nitrification inside the animal tissue. As shown by 16S rRNA gene analysis, the ascidian-associated Crenarchaeota were phylogenetically related to Nitrosopumilus maritimus, the first marine archaeon isolated in pure culture that grows chemolithoautotrophically oxidizing ammonia to nitrite aerobically. Catalysed reporter deposition (CARD)-FISH revealed that the Crenarchaeota were specifically located inside the tunic tissue of the colony, where moreover the expression of amoA gene was detected. The amoA gene encodes the alpha-subunit of ammonia monooxygenase, which is involved in the first step of nitrification, the oxidation of ammonia to nitrite. Sequencing of amoA gene showed that they were phylogenetically related to amoA genes of N. maritimus and other putative ammonia-oxidizing marine Crenarchaeota. In order to track the suspected nitrification activity inside the ascidian colony under in vivo conditions, microsensor profiles were measured through the tunic tissue. Net NO(x) production was detected in the tunic layer 1200-1800 microm with rates of 58-90 nmol cm(-3) h(-1). Oxygen and pH microsensor profiles showed that the layer of net NO(x) production coincided with O(2) concentrations of 103-116 microM and pH value of 5.2. Together, molecular and microsensor data indicate that Crenarchaeota could oxidize ammonia to nitrite aerobically, and thus be involved in nitrification inside the ascidian tissue.

journal_name

Environ Microbiol

authors

Martínez-García M,Stief P,Díaz-Valdés M,Wanner G,Ramos-Esplá A,Dubilier N,Antón J

doi

10.1111/j.1462-2920.2008.01761.x

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

2991-3001

issue

11

eissn

1462-2912

issn

1462-2920

pii

EMI1761

journal_volume

10

pub_type

杂志文章
  • Identification of inner membrane translocase components of TolC-mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::Cyanobacteria were the first organisms ever to perform oxygenic photosynthesis and still significantly contribute to primary production on a global scale. To assure the proper functioning of their primary metabolism and cell homeostasis, cyanobacteria must rely on efficient transport systems to cross their multilayere...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14095

    authors: Gonçalves CF,Pacheco CC,Tamagnini P,Oliveira P

    更新日期:2018-07-01 00:00:00

  • The geographical patterns of symbiont diversity in the invasive legume Mimosa pudica can be explained by the competitiveness of its symbionts and by the host genotype.

    abstract::Variations in the patterns of diversity of symbionts have been described worldwide on Mimosa pudica, a pan-tropical invasive species that interacts with both α and β-rhizobia. In this study, we investigated if symbiont competitiveness can explain these variations and the apparent prevalence of β- over α-rhizobia. We d...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12286

    authors: Melkonian R,Moulin L,Béna G,Tisseyre P,Chaintreuil C,Heulin K,Rezkallah N,Klonowska A,Gonzalez S,Simon M,Chen WM,James EK,Laguerre G

    更新日期:2014-07-01 00:00:00

  • High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library.

    abstract::The bacterial diversity of an industrial biofilter used for waste gas abatement in an animal-rendering plant was investigated. A 16S rDNA clone library was generated and 444 clones were screened using computer-aided amplified ribosomal DNA restriction analysis (ARDRA). Of the screened clones, 60.8% showed unique ARDRA...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00349.x

    authors: Friedrich U,Prior K,Altendorf K,Lipski A

    更新日期:2002-11-01 00:00:00

  • Probing the geological source and biological fate of hydrogen in Yellowstone hot springs.

    abstract::Hydrogen (H2 ) is enriched in hot springs and can support microbial primary production. Using a series of geochemical proxies, a model to describe variable H2 concentrations in Yellowstone National Park (YNP) hot springs is presented. Interaction between water and crustal iron minerals yields H2 that partition into th...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14730

    authors: Lindsay MR,Colman DR,Amenabar MJ,Fristad KE,Fecteau KM,Debes RV 2nd,Spear JR,Shock EL,Hoehler TM,Boyd ES

    更新日期:2019-10-01 00:00:00

  • Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content.

    abstract::Bioactive amounts of antibiotics as well as resistant bacteria reach the soil through manure fertilization. We investigated plasmids that may stimulate the environmental spread and interspecies transfer of antibiotic resistance. After treatment of two soils with manure, either with or without the sulfonamide antibioti...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01819.x

    authors: Heuer H,Kopmann C,Binh CT,Top EM,Smalla K

    更新日期:2009-04-01 00:00:00

  • The biosurfactant viscosin produced by Pseudomonas fluorescens SBW25 aids spreading motility and plant growth promotion.

    abstract::Food security depends on enhancing production and reducing loss to pests and pathogens. A promising alternative to agrochemicals is the use of plant growth-promoting rhizobacteria (PGPR), which are commonly associated with many, if not all, plant species. However, exploiting the benefits of PGPRs requires knowledge of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12469

    authors: Alsohim AS,Taylor TB,Barrett GA,Gallie J,Zhang XX,Altamirano-Junqueira AE,Johnson LJ,Rainey PB,Jackson RW

    更新日期:2014-07-01 00:00:00

  • Effect of energy deprivation on metabolite release by anaerobic marine naphthalene-degrading sulfate-reducing bacteria.

    abstract::The aromatic hydrocarbon naphthalene, which occurs in coal and oil, can be degraded by aerobic or anaerobic microorganisms. A wide-spread electron acceptor for the latter is sulfate. Evidence for in situ naphthalene degradation stems in particular from the detection of 2-naphthoate and [5,6,7,8]-tetrahydro-2-naphthoat...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15195

    authors: Chen G,Widdel F,Musat F

    更新日期:2020-09-01 00:00:00

  • Overproduction of the multidrug efflux pump MexEF-OprN does not impair Pseudomonas aeruginosa fitness in competition tests, but produces specific changes in bacterial regulatory networks.

    abstract::It is generally assumed that acquisition of antibiotic resistance leads to non-specific fitness costs. We have tested the alternative hypothesis that acquisition of antibiotic resistance may not always produce a general burden to the microorganisms, as measured in competition tests, but rather lead to specific changes...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02727.x

    authors: Olivares J,Alvarez-Ortega C,Linares JF,Rojo F,Köhler T,Martínez JL

    更新日期:2012-08-01 00:00:00

  • A high-throughput and quantitative hierarchical oligonucleotide primer extension (HOPE)-based approach to identify sources of faecal contamination in water bodies.

    abstract::As faecal contamination of recreational and drinking water impairs the water quality and threatens public health, water bodies are routinely monitored for faecal coliforms to detect contamination. However, faecal coliforms are facultative anaerobes that survive and reproduce in ambient waters, and their presence does ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01892.x

    authors: Hong PY,Wu JH,Liu WT

    更新日期:2009-07-01 00:00:00

  • Distribution of aerobic anoxygenic phototrophs in temperate freshwater systems.

    abstract::The presence of aerobic anoxygenic phototrophs (AAPs) was recently reported from various marine environments; however, there is little information regarding their distribution in fresh waters. We surveyed a number of freshwater systems in central Europe, by infra-red fluorometry, infra-red epifluorescence microscopy, ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01615.x

    authors: Masín M,Nedoma J,Pechar L,Koblízek M

    更新日期:2008-08-01 00:00:00

  • Changes in population structure of the soilborne fungus Gaeumannomyces graminis var. tritici during continuous wheat cropping.

    abstract::A method was developed to assess the genetic structure of Gaeumannomyces graminis var. tritici (Ggt) populations and test the hypothesis of an association between disease level in the field with changes in pathogen populations. A long-term wheat monoculture experiment, established since 1994, generated different take-...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00637.x

    authors: Lebreton L,Lucas P,Dugas F,Guillerm AY,Schoeny A,Sarniguet A

    更新日期:2004-11-01 00:00:00

  • Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic.

    abstract::The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of D- and L-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35 degrees N to 5 degrees S). Fluorescence in situ hybridization (FISH) rev...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01627.x

    authors: Varela MM,van Aken HM,Herndl GJ

    更新日期:2008-07-01 00:00:00

  • Asaia, a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders.

    abstract::Bacterial symbionts of insects have been proposed for blocking transmission of vector-borne pathogens. However, in many vector models the ecology of symbionts and their capability of cross-colonizing different hosts, an important feature in the symbiotic control approach, is poorly known. Here we show that the acetic ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02048.x

    authors: Crotti E,Damiani C,Pajoro M,Gonella E,Rizzi A,Ricci I,Negri I,Scuppa P,Rossi P,Ballarini P,Raddadi N,Marzorati M,Sacchi L,Clementi E,Genchi M,Mandrioli M,Bandi C,Favia G,Alma A,Daffonchio D

    更新日期:2009-12-01 00:00:00

  • Oxygen-dependent autoaggregation in Shewanella oneidensis MR-1.

    abstract::In aerobic chemostat cultures maintained at 50% dissolved O(2) tension (3.5 mg l(-1) dissolved O(2)), Shewanella oneidensis strain MR-1 rapidly aggregated upon addition of 0.68 mM CaCl(2) and retained this multicellular phenotype at high dilution rates. Confocal microscopy analysis of the extracellular matrix material...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01608.x

    authors: McLean JS,Pinchuk GE,Geydebrekht OV,Bilskis CL,Zakrajsek BA,Hill EA,Saffarini DA,Romine MF,Gorby YA,Fredrickson JK,Beliaev AS

    更新日期:2008-07-01 00:00:00

  • Chlorobaculum tepidum growth on biogenic S(0) as the sole photosynthetic electron donor.

    abstract::The green sulfur bacteria, the Chlorobi, are phototrophic bacteria that oxidize sulfide and deposit extracellular elemental sulfur globules [S(0)]. These are subsequently consumed after sulfide is exhausted. S(0) globules from a Chlorobaculum tepidum mutant strain were purified and used to show that the wild-type stra...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12995

    authors: Hanson TE,Bonsu E,Tuerk A,Marnocha CL,Powell DH,Chan CS

    更新日期:2016-09-01 00:00:00

  • Tree species, spatial heterogeneity, and seasonality drive soil fungal abundance, richness, and composition in Neotropical rainforests.

    abstract::Tropical ecosystems remain poorly understood and this is particularly true for belowground soil fungi. Soil fungi may respond to plant identity when, for example, plants differentially allocate resources belowground. However, spatial and temporal heterogeneity in factors such as plant inputs, moisture, or nutrients ca...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13342

    authors: Kivlin SN,Hawkes CV

    更新日期:2016-12-01 00:00:00

  • Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage.

    abstract::Cultivation-independent surveys of ribosomal RNA genes have revealed the existence of novel microbial lineages, many with no known cultivated representatives. Ribosomal RNA-based analyses, however, often do not provide significant information beyond phylogenetic affiliation. Analysis of large genome fragments recovere...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00133.x

    authors: Béjà O,Suzuki MT,Koonin EV,Aravind L,Hadd A,Nguyen LP,Villacorta R,Amjadi M,Garrigues C,Jovanovich SB,Feldman RA,DeLong EF

    更新日期:2000-10-01 00:00:00

  • Use of the rotating wall vessel technology to study the effect of shear stress on growth behaviour of Pseudomonas aeruginosa PA01.

    abstract::The biofilm phenotype of Pseudomonas aeruginosa enables this opportunistic pathogen to develop resistance to the immune system and antimicrobial agents. Pseudomonas aeruginosa biofilms are generated under varying levels of shear stress, depending on the infection site. In the lung mucus of cystic fibrosis (CF) patient...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01631.x

    authors: Crabbé A,De Boever P,Van Houdt R,Moors H,Mergeay M,Cornelis P

    更新日期:2008-08-01 00:00:00

  • Using a genetic algorithm to drive a microbial ecosystem in a desirable direction.

    abstract::The functioning of natural microbial ecosystems is influenced by various biotic and abiotic conditions. The careful experimental manipulation of environmental conditions can drive microbial ecosystems toward exhibiting desirable types of functionality. Such manipulations can be systematically approached by viewing the...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01603.x

    authors: Vandecasteele FP,Crawford RL,Hess TF

    更新日期:2008-07-01 00:00:00

  • Diversity patterns of Rhizobiaceae communities inhabiting soils, root surfaces and nodules reveal a strong selection of rhizobial partners by legumes.

    abstract::Current knowledge about rhizobial diversity patterns in non-nodule habitats is scarce, limiting our understanding of basic aspects of rhizobial ecology like competitiveness for nodule occupancy and host effects on community structure. We used a combination of cultivation-dependent and independent approaches to analyse...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13061

    authors: Miranda-Sánchez F,Rivera J,Vinuesa P

    更新日期:2016-09-01 00:00:00

  • Nitrite concentration influences the population structure of Nitrospira-like bacteria.

    abstract::Chemolithoautotrophic nitrite oxidizers of the genus Nitrospira are a monophyletic but diverse group of organisms, are widely distributed in many natural habitats, and play a key role in nitrogen elimination during biological wastewater treatment. Phylogenetic analyses of cloned 16S rRNA genes and fluorescence in situ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01033.x

    authors: Maixner F,Noguera DR,Anneser B,Stoecker K,Wegl G,Wagner M,Daims H

    更新日期:2006-08-01 00:00:00

  • Bacterial community dynamics during polysaccharide degradation at contrasting sites in the Southern and Atlantic Oceans.

    abstract::The bacterial degradation of polysaccharides is central to marine carbon cycling, but little is known about the bacterial taxa that degrade specific marine polysaccharides. Here, bacterial growth and community dynamics were studied during the degradation of the polysaccharides chitin, alginate and agarose in microcosm...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12842

    authors: Wietz M,Wemheuer B,Simon H,Giebel HA,Seibt MA,Daniel R,Brinkhoff T,Simon M

    更新日期:2015-10-01 00:00:00

  • Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly.

    abstract::Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15058

    authors: Juottonen H,Fontaine L,Wurzbacher C,Drakare S,Peura S,Eiler A

    更新日期:2020-08-01 00:00:00

  • Ecological diversification in the Bacillus cereus Group.

    abstract::The Bacillus cereus Group comprises organisms that are widely distributed in the environment and are of health and economic interest. We demonstrate an 'ecotypic' structure of populations in the B. cereus Group using (i) molecular data from Fluorescent Amplified Fragment Length Polymorphism patterns, ribosomal gene se...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01495.x

    authors: Guinebretière MH,Thompson FL,Sorokin A,Normand P,Dawyndt P,Ehling-Schulz M,Svensson B,Sanchis V,Nguyen-The C,Heyndrickx M,De Vos P

    更新日期:2008-04-01 00:00:00

  • Opportunistic bacteria use quorum sensing to disturb coral symbiotic communities and mediate the occurrence of coral bleaching.

    abstract::Coral associated microorganisms, especially some opportunistic pathogens can utilize quorum-sensing (QS) signals to affect population structure and host health. However, direct evidence about the link between coral bleaching and dysbiotic microbiomes under QS regulation was lacking. Here, using 11 opportunistic bacter...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15009

    authors: Zhou J,Lin ZJ,Cai ZH,Zeng YH,Zhu JM,Du XP

    更新日期:2020-05-01 00:00:00

  • Resting spore formation of aphid-pathogenic fungus Pandora nouryi depends on the concentration of infective inoculum.

    abstract::Resting spore formation of some aphid-pathogenic Entomophthorales is important for the seasonal pattern of their prevalence and survival but this process is poorly understood. To explore the possible mechanism involved in the process, Pandora nouryi (obligate aphid pathogen) interacted with green peach aphid Myzus per...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01577.x

    authors: Huang ZH,Feng MG

    更新日期:2008-07-01 00:00:00

  • Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability.

    abstract::In the rhizosphere, phosphorus (P) levels are low because of P uptake into the roots. Rhizobacteria live on carbon (C) exuded from roots, and may contribute to plant nutrition by liberating P from organic compounds such as phytates. We isolated over 300 phytate (Na-inositol hexa-phosphate; Na-IHP)-utilizing bacterial ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00701.x

    authors: Unno Y,Okubo K,Wasaki J,Shinano T,Osaki M

    更新日期:2005-03-01 00:00:00

  • Structure and function of methanogenic microbial communities in sediments of Amazonian lakes with different water types.

    abstract::Tropical lake sediments are a significant source for the greenhouse gas methane. We studied function (pathway, rate) and structure (abundance, taxonomic composition) of the microbial communities (Bacteria, Archaea) leading to methane formation together with the main physicochemical characteristics in the sediments of ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13491

    authors: Ji Y,Angel R,Klose M,Claus P,Marotta H,Pinho L,Enrich-Prast A,Conrad R

    更新日期:2016-12-01 00:00:00

  • Campylobacter lari: genotype and antibiotic resistance of isolates from cattle, wildlife and water in an area of mixed dairy farmland in the United Kingdom.

    abstract::Campylobacter lari is a rare human pathogen most commonly associated with birds and shellfish. Little information has been published regarding its prevalence in other environments, or on its potential role as a reservoir of antibiotic resistance. In this study, we characterized 109 C. lari isolated from a range of hos...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01295.x

    authors: Leatherbarrow AJ,Griffiths R,Hart CA,Kemp R,Williams NJ,Diggle PJ,Wright EJ,Sutherst J,Houghton P,French NP

    更新日期:2007-07-01 00:00:00

  • Microbiota associated with Mollitrichosiphum aphids (Hemiptera: Aphididae: Greenideinae): diversity, host species specificity and phylosymbiosis.

    abstract::Symbiotic association is universal in nature, and an array of symbionts play a crucial part in host life history. Aphids and their diverse symbionts have become a good model system to study insect-symbiont interactions. Previous symbiotic diversity surveys have mainly focused on a few aphid clades, and the relative im...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15391

    authors: Qin M,Chen J,Xu S,Jiang L,Qiao G

    更新日期:2021-01-07 00:00:00