Abstract:
:Arthrobacter chlorophenolicus A6 is a Gram-positive, 4-chlorophenol-degrading soil bacterium that was recently shown to be an effective colonizer of plant leaf surfaces. The genetic basis for this phyllosphere competency is unknown. In this paper, we describe the genome-wide expression profile of A.chlorophenolicus on leaves of common bean (Phaseolus vulgaris) compared with growth on agar surfaces. In phyllosphere-grown cells, we found elevated expression of several genes known to contribute to epiphytic fitness, for example those involved in nutrient acquisition, attachment, stress response and horizontal gene transfer. A surprising result was the leaf-induced expression of a subset of the so-called cph genes for the degradation of 4-chlorophenol. This subset encodes the conversion of the phenolic compound hydroquinone to 3-oxoadipate, and was shown to be induced not only by 4-chlorophenol but also hydroquinone, its glycosylated derivative arbutin, and phenol. Small amounts of hydroquinone, but not arbutin or phenol, were detected in leaf surface washes of P.vulgaris by gas chromatography-mass spectrometry. Our findings illustrate the utility of genomics approaches for exploration and improved understanding of a microbial habitat. Also, they highlight the potential for phyllosphere-based priming of bacteria to stimulate pollutant degradation, which holds promise for the application of phylloremediation.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Scheublin TR,Deusch S,Moreno-Forero SK,Müller JA,van der Meer JR,Leveau JHdoi
10.1111/1462-2920.12375subject
Has Abstractpub_date
2014-07-01 00:00:00pages
2212-25issue
7eissn
1462-2912issn
1462-2920journal_volume
16pub_type
杂志文章abstract::Bacterial and archaeal assemblages have been studied in a multipond solar saltern using a range of microbial ecology techniques by four laboratories simultaneously. These include 16S rDNA sequencing from both denaturing gradient gel electrophoresis (DGGE) and clone libraries, and culturing methods. Water samples from ...
journal_title:Environmental microbiology
pub_type: 杂志文章
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journal_title:Environmental microbiology
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journal_title:Environmental microbiology
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pub_type: 杂志文章
doi:10.1111/1462-2920.15228
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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journal_title:Environmental microbiology
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doi:10.1111/1462-2920.13745
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journal_title:Environmental microbiology
pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13784
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journal_title:Environmental microbiology
pub_type: 杂志文章
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doi:10.1111/1462-2920.15122
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14095
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2006.01084.x
更新日期:2006-12-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13028
更新日期:2016-05-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.02027.x
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Environmental microbiology
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journal_title:Environmental microbiology
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pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13834
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