MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii.

Abstract:

:pH-responsive transcription factor of the PacC/Rim101 family governs adaptation to environment, development and virulence in many fungal pathogens. In this study, we report the functions of a PacC homologue, MrpacC, in an insect pathogenic fungus Metarhizium robertsii. The gene was highly transcribed in the fungus in alkaline conditions, and deletion of MrpacC impaired fungal responses to ambient pH and salt/metal challenges but not osmotic stress. We found that MrpacC is required for fungal full virulence by contributing to penetration of insect cuticles, mycosis of insect cadavers and evasion of host immunity. In MrpacC deletion strains, the chitinase but not protease activity was reduced, which was consistent with the downregulation of groups A and C chitinase genes. Further, the glucosyltransferase genes involved in cell wall remodelling and protein glycosylation were upregulated in ΔMrpacC. MrpacC transcriptional control of chitinase and glucosyltransferase genes was verified both by the presence of PacC consensus binding motif in gene promoter regions and the promoter DNA-binding assays. The results of this study not only advances the understanding of PacC function in fungal development and virulence, but will also facilitate future studies on the mechanism(s) behind the selective control of target genes by PacC.

journal_name

Environ Microbiol

authors

Huang W,Shang Y,Chen P,Gao Q,Wang C

doi

10.1111/1462-2920.12451

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

994-1008

issue

4

eissn

1462-2912

issn

1462-2920

journal_volume

17

pub_type

杂志文章
  • Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes.

    abstract::The severe environmental stresses of the Arctic may have promoted unique soil bacterial communities compared with those found in lower latitude environments. Here, we present a comprehensive analysis of the biogeography of soil bacterial communities in the Arctic using a high resolution bar-coded pyrosequencing techni...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02277.x

    authors: Chu H,Fierer N,Lauber CL,Caporaso JG,Knight R,Grogan P

    更新日期:2010-11-01 00:00:00

  • Lipopolysaccharide O-chain microheterogeneity of Salmonella serotypes Enteritidis and Typhimurium.

    abstract::Variability in the lipopolysaccharide (LPS) of the two most prevalent Salmonella serotypes causing food-borne salmonellosis was assessed using gas chromatography analysis of neutral sugars from 43 Salmonella enterica serovar Enteritidis (S. Enteritidis) and 20 Salmonella enterica serovar Typhimurium (S. Typhimurium) i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00200.x

    authors: Parker CT,Liebana E,Henzler DJ,Guard-Petter J

    更新日期:2001-05-01 00:00:00

  • Oligotrophic lagoons of the South Pacific Ocean are home to a surprising number of novel eukaryotic microorganisms.

    abstract::The diversity of microbial eukaryotes was surveyed by environmental sequencing from tropical lagoon sites of the South Pacific, collected through the American Museum of Natural History (AMNH)'s Explore21 expedition to the Solomon Islands in September 2013. The sampled lagoons presented low nutrient concentrations typi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13523

    authors: Kim E,Sprung B,Duhamel S,Filardi C,Kyoon Shin M

    更新日期:2016-12-01 00:00:00

  • Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota.

    abstract::Among human faecal bacteria, many members of the Clostridium leptum subgroup are fibrolytic and butyrate producing microorganisms thereby contributing to processes important to colonic health. Yet this phylogenetic subgroup remains poorly described to date. To improve detection and description of members of the C. lep...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00763.x

    authors: Lay C,Sutren M,Rochet V,Saunier K,Doré J,Rigottier-Gois L

    更新日期:2005-07-01 00:00:00

  • The role of antibiotics and antibiotic resistance in nature.

    abstract::Investigations of antibiotic resistance from an environmental prospective shed new light on a problem that was traditionally confined to a subset of clinically relevant antibiotic-resistant bacterial pathogens. It is clear that the environmental microbiota, even in apparently antibiotic-free environments, possess an e...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2009.01972.x

    authors: Aminov RI

    更新日期:2009-12-01 00:00:00

  • Double-tagged fluorescent bacterial bioreporter for the study of polycyclic aromatic hydrocarbon diffusion and bioavailability.

    abstract::Bacterial degradation of polycyclic aromatic hydrocarbons (PAHs), ubiquitous contaminants from oil and coal, is typically limited by poor accessibility of the contaminant to the bacteria. In order to measure PAH availability in complex systems, we designed a number of diffusion-based assays with a double-tagged bacter...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01952.x

    authors: Tecon R,Binggeli O,van der Meer JR

    更新日期:2009-09-01 00:00:00

  • Microbial succession of nitrate-reducing bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps.

    abstract::Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil and rhizosphere soil of Poa alpina was samp...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01051.x

    authors: Deiglmayr K,Philippot L,Tscherko D,Kandeler E

    更新日期:2006-09-01 00:00:00

  • Surviving the heat: heterogeneity of response in Saccharomyces cerevisiae provides insight into thermal damage to the membrane.

    abstract::Environmental heat stress impacts on the physiology and viability of microbial cells with concomitant implications for microbial activity and diversity. Previously, it has been demonstrated that gradual heating of Saccharomyces cerevisiae induces a degree of thermal resistance, whereas a heat shock results in a high l...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12866

    authors: Guyot S,Gervais P,Young M,Winckler P,Dumont J,Davey HM

    更新日期:2015-08-01 00:00:00

  • The role of single subunits of the DNA transport machinery of Thermus thermophilus HB27 in DNA binding and transport.

    abstract::Thermus thermophilus HB27 is well known for its extraordinary trait of high frequencies of natural transformation, which is considered a major mechanism of horizontal gene transfer. We show that the DNA translocator of T. thermophilus binds and transports DNA from members of all three domains. These results, together ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01801.x

    authors: Schwarzenlander C,Haase W,Averhoff B

    更新日期:2009-04-01 00:00:00

  • Pirellula and OM43 are among the dominant lineages identified in an Oregon coast diatom bloom.

    abstract::Although bacterioplankton and phytoplankton are generally perceived as closely linked in marine systems, specific interactions between discrete bacterioplankton and phytoplankton populations are largely unknown. However, measurements of bacterioplankton distributions during phytoplankton blooms may indicate specific m...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01029.x

    authors: Morris RM,Longnecker K,Giovannoni SJ

    更新日期:2006-08-01 00:00:00

  • Escherichia coli has multiple enzymes that attack TNT and release nitrogen for growth.

    abstract::There has been a growing interest in the degradation of 2,4,6-trinitrotoluene (TNT) over the last decade, ever since its removal from polluted sites was declared an international environmental priority. Certain aerobic and anaerobic microorganisms are capable of using TNT as an N source, although very few studies have...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01272.x

    authors: González-Pérez MM,van Dillewijn P,Wittich RM,Ramos JL

    更新日期:2007-06-01 00:00:00

  • Susceptibility of Vibrio aestuarianus 01/032 to the antibacterial activity of Mytilus haemolymph: identification of a serum opsonin involved in mannose-sensitive interactions.

    abstract::The interactions of Vibrio aestuarianus 01/032 with haemolymph of the bivalves Mytilus galloprovincialis and Crassostrea gigas were investigated to understand if haemolymph components (haemocytes and soluble factors) could be involved in the higher resistance to microbial infection shown by mussels in comparison with ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12750

    authors: Pezzati E,Canesi L,Damonte G,Salis A,Marsano F,Grande C,Vezzulli L,Pruzzo C

    更新日期:2015-11-01 00:00:00

  • Ectomycorrhizal fungi inoculation alleviates simulated acid rain effects on soil ammonia oxidizers and denitrifiers in Masson pine forest.

    abstract::Acid rain can cause severe effects on soil biota and nutrient biogeochemical cycles in the forest ecosystem, but how plant-symbiotic ectomycorrhizal fungi will modulate the effects remains unknown. Here, we conducted a full factorial field experiment in a Masson pine forest by simultaneously controlling the acidity of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14457

    authors: Li Y,Chen Z,He JZ,Wang Q,Shen C,Ge Y

    更新日期:2019-01-01 00:00:00

  • Future warming and acidification result in multiple ecological impacts to a temperate coralline alga.

    abstract::Coralline algae are a crucial component of reef systems, stabilising reef substrate, providing habitat and contributing to accretion. Coralline algae and their surface microbial biofilms are also important as settlement cues for marine invertebrates, yet few studies address the impact of future environmental condition...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14113

    authors: Huggett MJ,McMahon K,Bernasconi R

    更新日期:2018-08-01 00:00:00

  • Geomicrobiological exploration and characterization of a novel deep-sea hydrothermal system at the TOTO caldera in the Mariana Volcanic Arc.

    abstract::Novel hydrothermal activities accompanying effluent white smokers and elemental sulfur chimney structures at the north-east lava dome of the TOTO caldera depression in the Mariana Volcanic Arc have been explored and characterized by geochemical and microbiological surveys. White smoker hydrothermal fluids were observe...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00884.x

    authors: Nakagawa T,Takai K,Suzuki Y,Hirayama H,Konno U,Tsunogai U,Horikoshi K

    更新日期:2006-01-01 00:00:00

  • Relative contributions of archaea and bacteria to aerobic ammonia oxidation in the environment.

    abstract::Traditionally, organisms responsible for major biogeochemical cycling processes have been determined by physiological characterization of environmental isolates in laboratory culture. Molecular techniques have, however, confirmed the widespread occurrence of abundant bacterial and archaeal groups with no cultivated re...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2008.01775.x

    authors: Prosser JI,Nicol GW

    更新日期:2008-11-01 00:00:00

  • Recognition cascade and metabolite transfer in a marine bacteria-phytoplankton model system.

    abstract::The trophic linkage between marine bacteria and phytoplankton in the surface ocean is a key step in the global carbon cycle, with almost half of marine primary production transformed by heterotrophic bacterioplankton within hours to weeks of fixation. Early studies conceptualized this link as the passive addition and ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13834

    authors: Durham BP,Dearth SP,Sharma S,Amin SA,Smith CB,Campagna SR,Armbrust EV,Moran MA

    更新日期:2017-09-01 00:00:00

  • Identifying pioneer bacterial species responsible for biofouling membrane bioreactors.

    abstract::More effective control of membrane biofouling in membrane bioreactors (MBRs) lies in the fundamental understanding of the pioneer microorganisms responsible for surface colonization that leads to biofilm formation. In this study, the composition of the planktonic and sessile microbial communities inhabiting four labor...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00909.x

    authors: Zhang K,Choi H,Dionysiou DD,Sorial GA,Oerther DB

    更新日期:2006-03-01 00:00:00

  • The tail-associated depolymerase of Erwinia amylovora phage L1 mediates host cell adsorption and enzymatic capsule removal, which can enhance infection by other phage.

    abstract::The depolymerase enzyme (DpoL1) encoded by the T7-like phage L1 efficiently degrades amylovoran, an important virulence factor and major component of the extracellular polysaccharide (EPS) of its host, the plant pathogen Erwinia amylovora. Mass spectrometry analysis of hydrolysed EPS revealed that DpoL1 cleaves the ga...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12212

    authors: Born Y,Fieseler L,Klumpp J,Eugster MR,Zurfluh K,Duffy B,Loessner MJ

    更新日期:2014-07-01 00:00:00

  • PAAR-Rhs proteins harbor various C-terminal toxins to diversify the antibacterial pathways of type VI secretion systems.

    abstract::The type VI secretion system (T6SS) of bacteria plays a key role in competing for specific niches by the contact-dependent killing of competitors. Recently, Rhs proteins with polymorphic C-terminal toxin-domains that inhibit or kill neighboring cells were identified. In this report, we identified a novel Rhs with an M...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13621

    authors: Ma J,Sun M,Dong W,Pan Z,Lu C,Yao H

    更新日期:2017-01-01 00:00:00

  • Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism.

    abstract::Interaction between microbes affects the growth, metabolism and differentiation of members of the microbial community. While direct and indirect competition, like antagonism and nutrient consumption have a negative effect on the interacting members of the population, microbes have also evolved in nature not only to fi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12564

    authors: Benoit I,van den Esker MH,Patyshakuliyeva A,Mattern DJ,Blei F,Zhou M,Dijksterhuis J,Brakhage AA,Kuipers OP,de Vries RP,Kovács ÁT

    更新日期:2015-06-01 00:00:00

  • Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes.

    abstract::The marine bacterium Vibrio fischeri is the monospecific symbiont of the Hawaiian bobtail squid, Euprymna scolopes, and the establishment of this association involves a number of signaling pathways and transcriptional responses between both partners. We report here the first full RNA-Seq dataset representing host-asso...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13684

    authors: Thompson LR,Nikolakakis K,Pan S,Reed J,Knight R,Ruby EG

    更新日期:2017-05-01 00:00:00

  • Energy flux controls tetraether lipid cyclization in Sulfolobus acidocaldarius.

    abstract::Microorganisms regulate the composition of their membranes in response to environmental cues. Many Archaea maintain the fluidity and permeability of their membranes by adjusting the number of cyclic moieties within the cores of their glycerol dibiphytanyl glycerol tetraether (GDGT) lipids. Cyclized GDGTs increase memb...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14851

    authors: Zhou A,Weber Y,Chiu BK,Elling FJ,Cobban AB,Pearson A,Leavitt WD

    更新日期:2020-01-01 00:00:00

  • A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality.

    abstract::Biological membranes have evolved different mechanisms to modify their composition in response to chemical stimuli in a process called 'homeoviscous adaptation'. Among these mechanisms, modifications in the ratio of saturated/unsaturated fatty acids and in cis/trans fatty acid isomers, cyclopropanation and changes in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01236.x

    authors: Bernal P,Muñoz-Rojas J,Hurtado A,Ramos JL,Segura A

    更新日期:2007-05-01 00:00:00

  • Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant.

    abstract::The complex ecology of free-living amoebae (FLA) and their role in spreading pathogenic microorganisms through water systems have recently raised considerable interest. In this study, we investigated the presence of FLA and amoebae-resisting bacteria (ARB) at various stages of a drinking water plant fed with river wat...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01693.x

    authors: Thomas V,Loret JF,Jousset M,Greub G

    更新日期:2008-10-01 00:00:00

  • Redox cycling of arsenic by the hydrothermal marine bacterium Marinobacter santoriniensis.

    abstract::Marinobacter santoriniensis NKSG1(T) is a mesophilic, dissimilatory arsenate-reducing and arsenite-oxidizing bacterium isolated from an arsenate-reducing enrichment culture. The inoculum was obtained from arsenic-rich shallow marine hydrothermal sediment from Santorini, Greece, with evidence of arsenic redox cycling. ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01890.x

    authors: Handley KM,Héry M,Lloyd JR

    更新日期:2009-06-01 00:00:00

  • Three transcription regulators of the Nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in Wolinella succinogenes.

    abstract::Sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (NO) or nitrous oxide (N2 O) in the environment and subsequent upregulation of corresponding catabolic enzymes is essential for many microbial cells. The molecular mechanisms of such adaptive responses ar...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13060

    authors: Kern M,Simon J

    更新日期:2016-09-01 00:00:00

  • Transcriptional response of the obligate anaerobe Desulfuribacillus stibiiarsenatis MLFW-2T to growth on antimonate and other terminal electron acceptors.

    abstract::Enzymes of the dimethyl sulfoxide reductase (DMSOR) family catalyse two-electron redox reactions pivotal to the dissimilatory metabolism of a variety of organic and inorganic compounds. The draft genome of the obligately anaerobic bacterium Desulfuribacillus stibiiarsenatis MLFW-2T contains 14 genes that are predicted...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14503

    authors: Abin CA,Hollibaugh JT

    更新日期:2019-02-01 00:00:00

  • Diverse seed banks favour adaptation of microalgal populations to future climate conditions.

    abstract::Selection of suitable genotypes from diverse seed banks may help phytoplankton populations to cope with environmental changes. This study examines whether the high genotypic diversity found in the Baltic cyst pool of the toxic dinoflagellate Alexandrium ostenfeldii is coupled to phenotypic variability that could aid s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13070

    authors: Kremp A,Oja J,LeTortorec AH,Hakanen P,Tahvanainen P,Tuimala J,Suikkanen S

    更新日期:2016-02-01 00:00:00

  • Transcription of a 'photosynthetic' T4-type phage during infection of a marine cyanobacterium.

    abstract::The transcription of S-PM2 phage following infection of Synechococcus sp. WH7803, a marine cyanobacterium, was analysed by quantitative real-time PCR. Unlike the distantly related coliphage T4, there were only two (early and late) instead of three (early, middle and late) classes of transcripts during the developmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00969.x

    authors: Clokie MR,Shan J,Bailey S,Jia Y,Krisch HM,West S,Mann NH

    更新日期:2006-05-01 00:00:00