Lipopolysaccharide O-chain microheterogeneity of Salmonella serotypes Enteritidis and Typhimurium.

Abstract:

:Variability in the lipopolysaccharide (LPS) of the two most prevalent Salmonella serotypes causing food-borne salmonellosis was assessed using gas chromatography analysis of neutral sugars from 43 Salmonella enterica serovar Enteritidis (S. Enteritidis) and 20 Salmonella enterica serovar Typhimurium (S. Typhimurium) isolates. Four substantially different types of O-chain chemotypes were detected using cluster analysis of sugar compositions; these were low-molecular-mass (LMM) LPS, glucosylated LMM LPS, high-molecular-mass (HMM) LPS and glucosylated HMM LPS. Nineteen out of 20 S. Typhimurium isolates yielded glucosylated LMM. In contrast, S. Enteritidis produced a more diverse structure, which varied according to the source and history of the isolate: 45.5% of egg isolates yielded glucosylated HMM LPS; 100% of stored strains lacked glucosylation but retained chain length in some cases; and 83.3% of fresh isolates from the naturally infected house mouse Mus musculus produced glucosylated LMM LPS. A chain length determinant (wzz) mutant of S. Enteritidis produced a structure similar to that of S. Typhimurium and was used to define what constituted significant differences in structure using cluster analysis. Fine mapping of the S. Enteritidis chromosome by means of a two-restriction enzyme-ribotyping technique suggested that mouse isolates producing glucosylated LMM LPS were closely related to orally invasive strains obtained from eggs, and that stored strains were accumulating genetic changes that correlated with suppression of LPS O-chain glucosylation. These results suggest that the determination of LPS chemotype is a useful tool for epidemiological monitoring of S. Enteritidis, which displays an unusual degree of diversity in its LPS O-chain.

journal_name

Environ Microbiol

authors

Parker CT,Liebana E,Henzler DJ,Guard-Petter J

doi

10.1046/j.1462-2920.2001.00200.x

keywords:

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

332-42

issue

5

eissn

1462-2912

issn

1462-2920

pii

emi200

journal_volume

3

pub_type

杂志文章
  • Nitrogen fixation by a marine non-heterocystous cyanobacterium requires a heterotrophic bacterial consort.

    abstract::Cultures of the non-heterocystous cyanobacterium, Leptolyngbya nodulosa, could be grown indefinitely in media devoid of combined nitrogen. Acetylene reduction assays showed that these cultures fixed nitrogen in the dark period of a diurnal cycle under micro-oxygenic or anaerobic conditions. Addition of DCMU to culture...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02159.x

    authors: Li Z,Yu J,Kim KR,Brand J

    更新日期:2010-05-01 00:00:00

  • The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production.

    abstract::Syntrophomonas wolfei is a specialist, evolutionarily adapted for syntrophic growth with methanogens and other hydrogen- and/or formate-using microorganisms. This slow-growing anaerobe has three putative ribosome RNA operons, each of which has 16S rRNA and 23S rRNA genes of different length and multiple 5S rRNA genes....

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02237.x

    authors: Sieber JR,Sims DR,Han C,Kim E,Lykidis A,Lapidus AL,McDonnald E,Rohlin L,Culley DE,Gunsalus R,McInerney MJ

    更新日期:2010-08-01 00:00:00

  • Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly.

    abstract::Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15058

    authors: Juottonen H,Fontaine L,Wurzbacher C,Drakare S,Peura S,Eiler A

    更新日期:2020-08-01 00:00:00

  • Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron.

    abstract::Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing condit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15328

    authors: D'Agostino PM,Yeung ACY,Poljak A,David Waite T,Neilan BA

    更新日期:2021-01-01 00:00:00

  • Seasonality and monthly dynamics of marine myovirus communities.

    abstract::Marine myoviruses (i.e. bacteriophages with a contractile tail sheath) are numerically abundant and genetically diverse. We developed a terminal restriction fragment length polymorphism assay (TRFLP) for g23, the conserved gene encoding the major capsid protein, to investigate T4-like myovirus communities at USC's Mic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02744.x

    authors: Chow CE,Fuhrman JA

    更新日期:2012-08-01 00:00:00

  • A periplasmic arsenite-binding protein involved in regulating arsenite oxidation.

    abstract::Arsenic (As) is the most common toxic element in the environment, ranking first on the Superfund List of Hazardous Substances. Microbial redox transformations are the principal drivers of As chemical speciation, which in turn dictates As mobility and toxicity. Consequently, in order to manage or remediate environmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02672.x

    authors: Liu G,Liu M,Kim EH,Maaty WS,Bothner B,Lei B,Rensing C,Wang G,McDermott TR

    更新日期:2012-07-01 00:00:00

  • Heterotrophic bacteria from an extremely phosphate-poor lake have conditionally reduced phosphorus demand and utilize diverse sources of phosphorus.

    abstract::Heterotrophic Proteobacteria and Actinobacteria were isolated from Lake Matano, Indonesia, a stratified, ferruginous (iron-rich), ultra-oligotrophic lake with phosphate concentrations below 50 nM. Here, we describe the growth of eight strains of heterotrophic bacteria on a variety of soluble and insoluble sources of p...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13063

    authors: Yao M,Elling FJ,Jones C,Nomosatryo S,Long CP,Crowe SA,Antoniewicz MR,Hinrichs KU,Maresca JA

    更新日期:2016-02-01 00:00:00

  • Functional surface proteomic profiling reveals the host heat-shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages.

    abstract::Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species of the order Mucorales. Immune cells of the innate immune system serve as the first line of defence against inhaled spores. Alveolar macrophages were challenged with the mucoralean fungus Lichtheimia corymbifera an...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15140

    authors: Hassan MIA,Kruse JM,Krüger T,Dahse HM,Cseresnyés Z,Blango MG,Slevogt H,Hörhold F,Ast V,König R,Figge MT,Kniemeyer O,Brakhage AA,Voigt K

    更新日期:2020-09-01 00:00:00

  • Isotope analysis as a natural reaction probe to determine mechanisms of biodegradation of 1,2-dichloroethane.

    abstract::1,2-Dichloroethane (1,2-DCA), a chlorinated aliphatic hydrocarbon, is a well-known groundwater contaminant. In this study, fractionation of stable carbon isotope values of 1,2-DCA during biodegradation was used as a novel reaction probe to provide information about the mechanism of 1,2-DCA biodegradation under both ae...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01282.x

    authors: Hirschorn SK,Dinglasan-Panlilio MJ,Edwards EA,Lacrampe-Couloume G,Sherwood Lollar B

    更新日期:2007-07-01 00:00:00

  • Molecular characterization of thermostable direct haemolysin-related haemolysin (TRH)-positive Vibrio parahaemolyticus from oysters in Mangalore, India.

    abstract::Pathogenic Vibrio parahaemolyticus strains producing either or both of a thermostable direct haemolysin (TDH) and a TDH-related haemolysin (TRH) encoded by tdh and trh genes, respectively, are isolated at a low rate from the environment. However, recently we observed that a considerable percentage of APW (alkaline pep...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.00990.x

    authors: Parvathi A,Kumar HS,Bhanumathi A,Ishibashi M,Nishibuchi M,Karunasagar I,Karunasagar I

    更新日期:2006-06-01 00:00:00

  • Functional analysis of the Pseudomonas syringae rulAB determinant in tolerance to ultraviolet B (290-320 nm) radiation and distribution of rulAB among P. syringae pathovars.

    abstract::The effect of the plasmid-encoded rulAB (resistance to ultraviolet radiation) determinant on responses of Pseudomonas syringae to ultraviolet-B (UV-B) radiation and the distribution of rulAB among pathovars of P. syringae were determined. The cloned rulAB determinant and the native rulAB+ plasmid pPSR1 both conferred ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.1999.00008.x

    authors: Sundin GW,Murillo J

    更新日期:1999-02-01 00:00:00

  • Enterohaemorrhagic Escherichia coli haemolysin is cleaved and inactivated by serine protease EspPα.

    abstract::The haemolysin from enterohaemorrhagic Escherichia coli (EHEC-Hly) and the serine protease EspPα are putative virulence factors of EHEC. We investigated the interplay between these secreted factors and demonstrate that EspPα cleaves the 107 kDa large EHEC-Hly. Degradation was observed when purified EspPα was added to ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02431.x

    authors: Brockmeyer J,Aldick T,Soltwisch J,Zhang W,Tarr PI,Weiss A,Dreisewerd K,Müthing J,Bielaszewska M,Karch H

    更新日期:2011-05-01 00:00:00

  • Bacterial pathogens, virulence mechanism and host defence in marine macroalgae.

    abstract::Macroalgae are important ecosystem engineers in temperate marine waters. The function of macroalgae is intimately linked to the composition and structure of their epibiotic bacterial, communities, and evidence has emerged that bacteria can also have a negative impact on their host by causing disease. A few examples ex...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.12288

    authors: Egan S,Fernandes ND,Kumar V,Gardiner M,Thomas T

    更新日期:2014-04-01 00:00:00

  • The genome and structural proteome of an ocean siphovirus: a new window into the cyanobacterial 'mobilome'.

    abstract::Prochlorococcus, an abundant phototroph in the oceans, are infected by members of three families of viruses: myo-, podo- and siphoviruses. Genomes of myo- and podoviruses isolated on Prochlorococcus contain DNA replication machinery and virion structural genes homologous to those from coliphages T4 and T7 respectively...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02081.x

    authors: Sullivan MB,Krastins B,Hughes JL,Kelly L,Chase M,Sarracino D,Chisholm SW

    更新日期:2009-11-01 00:00:00

  • Relative contributions of archaea and bacteria to aerobic ammonia oxidation in the environment.

    abstract::Traditionally, organisms responsible for major biogeochemical cycling processes have been determined by physiological characterization of environmental isolates in laboratory culture. Molecular techniques have, however, confirmed the widespread occurrence of abundant bacterial and archaeal groups with no cultivated re...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2008.01775.x

    authors: Prosser JI,Nicol GW

    更新日期:2008-11-01 00:00:00

  • Selection in the host structures the microbiota associated with developing cod larvae (Gadus morhua).

    abstract::Marine fish larvae are immature upon hatching, and share their environment with high numbers of bacteria. The microbial communities associated with developing fish larvae might be structured by other factors than those important in developing terrestrial animals. Here, we analysed the beta (β)-diversity of the microbi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12888

    authors: Bakke I,Coward E,Andersen T,Vadstein O

    更新日期:2015-10-01 00:00:00

  • Chromomycin, an antibiotic produced by Streptomyces flaviscleroticus might play a role in the resistance to oxidative stress and is essential for viability in stationary phase.

    abstract::The well-known role of antibiotics in killing sensitive organisms has been challenged by the effects they exert at subinhibitory concentrations. Unfortunately, there are very few published reports on the advantages these molecules may confer to their producers. This study describes the construction of a genetically ve...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14515

    authors: Prajapati D,Kumari N,Dave K,Chatupale V,Pohnerkar J

    更新日期:2019-02-01 00:00:00

  • Physiological and cell morphology adaptation of Bacillus subtilis at near-zero specific growth rates: a transcriptome analysis.

    abstract::Nutrient scarcity is a common condition in nature, but the resulting extremely low growth rates (below 0.025 h(-1) ) are an unexplored research area in Bacillus subtilis. To understand microbial life in natural environments, studying the adaptation of B. subtilis to near-zero growth conditions is relevant. To this end...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12676

    authors: Overkamp W,Ercan O,Herber M,van Maris AJ,Kleerebezem M,Kuipers OP

    更新日期:2015-02-01 00:00:00

  • Asaia, a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders.

    abstract::Bacterial symbionts of insects have been proposed for blocking transmission of vector-borne pathogens. However, in many vector models the ecology of symbionts and their capability of cross-colonizing different hosts, an important feature in the symbiotic control approach, is poorly known. Here we show that the acetic ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02048.x

    authors: Crotti E,Damiani C,Pajoro M,Gonella E,Rizzi A,Ricci I,Negri I,Scuppa P,Rossi P,Ballarini P,Raddadi N,Marzorati M,Sacchi L,Clementi E,Genchi M,Mandrioli M,Bandi C,Favia G,Alma A,Daffonchio D

    更新日期:2009-12-01 00:00:00

  • Complex nitrogen cycling in the sponge Geodia barretti.

    abstract::Marine sponges constitute major parts of coral reefs and deep-water communities. They often harbour high amounts of phylogenetically and physiologically diverse microbes, which are so far poorly characterized. Many of these sponges regulate their internal oxygen concentration by modulating their ventilation behaviour ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01944.x

    authors: Hoffmann F,Radax R,Woebken D,Holtappels M,Lavik G,Rapp HT,Schläppy ML,Schleper C,Kuypers MM

    更新日期:2009-09-01 00:00:00

  • Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes.

    abstract::Technologies for massively parallel sequencing are revolutionizing microbial ecology and are vastly increasing the scale of ribosomal RNA (rRNA) gene studies. Although pyrosequencing has increased the breadth and depth of possible rRNA gene sampling, one drawback is that the number of reads obtained per sample is diff...

    journal_title:Environmental microbiology

    pub_type: 信件

    doi:10.1111/j.1462-2920.2011.02550.x

    authors: Gihring TM,Green SJ,Schadt CW

    更新日期:2012-02-01 00:00:00

  • Temperature drives the assembly of endophytic communities' seasonal succession.

    abstract::Endophytic microorganisms asymptomatically colonise plant tissues. Exploring the assembly dynamics of bacterial endophytic communities is essential to understand the functioning of the plant holobiont and to optimise their possible use as biopesticides or plant biostimulants. The variation in endophytic communities in...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13843

    authors: Campisano A,Albanese D,Yousaf S,Pancher M,Donati C,Pertot I

    更新日期:2017-08-01 00:00:00

  • A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite.

    abstract::Serpentinization is the hydration and oxidation of ultramafic rock, which occurs as oceanic lithosphere is emplaced onto continental margins (ophiolites), and along the seafloor as faulting exposes this mantle-derived material to circulating hydrothermal fluids. This process leads to distinctive fluid chemistries as m...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15006

    authors: Sabuda MC,Brazelton WJ,Putman LI,McCollom TM,Hoehler TM,Kubo MDY,Cardace D,Schrenk MO

    更新日期:2020-06-01 00:00:00

  • In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.

    abstract::Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-canonical amino acid...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12436

    authors: Hatzenpichler R,Scheller S,Tavormina PL,Babin BM,Tirrell DA,Orphan VJ

    更新日期:2014-08-01 00:00:00

  • Some have it all: multicellularity, magnetotaxis and photokinesis in one bacterium.

    abstract::Bacteria developed many different ways to orient themselves in the environment. Magnetoreception with following motility along Earth's magnetic field lines and photoreception with subsequent positive or negative phototaxis allow bacteria to optimally position themselves for survival and growth. Some bacteria show both...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15157

    authors: Wettstadt S

    更新日期:2020-08-01 00:00:00

  • The new tools revolutionizing Vibrio science.

    abstract::As microbiologists we live in exciting times. A variety of technical and conceptual developments, particularly in the last decade have revolutionized the field of microbiology, redrawing the landscape, and entirely redefining what is possible. Perhaps this paradigm shift is no more apparent than in the study of vibrio...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15083

    authors: Baker-Austin C,Trinanes J,Martinez-Urtaza J

    更新日期:2020-10-01 00:00:00

  • A multiplayer game: species of Clostridium, Acinetobacter, and Pseudomonas are responsible for the persistence of antibiotic resistance genes in manure-treated soils.

    abstract::Antibiotics are routinely used in modern livestock farming. The manure from medicated animals is used for the fertilization of arable crops, which in turn leads to the accumulation of antibiotic resistance genes (ARGs) in the environment. This is a potentially serious public health issue, yet the identities of the bac...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13337

    authors: Leclercq SO,Wang C,Sui Z,Wu H,Zhu B,Deng Y,Feng J

    更新日期:2016-10-01 00:00:00

  • Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability.

    abstract::In the rhizosphere, phosphorus (P) levels are low because of P uptake into the roots. Rhizobacteria live on carbon (C) exuded from roots, and may contribute to plant nutrition by liberating P from organic compounds such as phytates. We isolated over 300 phytate (Na-inositol hexa-phosphate; Na-IHP)-utilizing bacterial ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00701.x

    authors: Unno Y,Okubo K,Wasaki J,Shinano T,Osaki M

    更新日期:2005-03-01 00:00:00

  • Widespread detection of Candidatus Accumulibacter phosphatis, a polyphosphate-accumulating organism, in sediments of the Columbia River estuary.

    abstract::Enhanced biological phosphorus removal (EBPR) exploits the metabolism of polyphosphate-accumulating organisms (PAOs) to remove excess phosphorus (P) from wastewater treatment. Candidatus Accumulibacter phosphatis (Accumulibacter) is the most abundant and well-studied PAO in EBPR systems. In a previous study, we detect...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14576

    authors: Watson SJ,Needoba JA,Peterson TD

    更新日期:2019-04-01 00:00:00

  • Persister cells resuscitate via ribosome modification by 23S rRNA pseudouridine synthase RluD.

    abstract::Upon a wide range of stress conditions (e.g. nutrient, antibiotic, oxidative), a subpopulation of bacterial cells known as persisters survives by halting metabolism. These cells resuscitate rapidly to reconstitute infections once the stress is removed and nutrients are provided. However, how these dormant cells resusc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14828

    authors: Song S,Wood TK

    更新日期:2020-03-01 00:00:00