Redox cycling of arsenic by the hydrothermal marine bacterium Marinobacter santoriniensis.

Abstract:

:Marinobacter santoriniensis NKSG1(T) is a mesophilic, dissimilatory arsenate-reducing and arsenite-oxidizing bacterium isolated from an arsenate-reducing enrichment culture. The inoculum was obtained from arsenic-rich shallow marine hydrothermal sediment from Santorini, Greece, with evidence of arsenic redox cycling. Growth studies demonstrated M. santoriniensis NKSG1(T) is capable of conserving energy from the reduction of arsenate [As(V)] with acetate or lactate as the electron donor, and of oxidizing arsenite [As(III)] heterotrophically with oxygen as the electron acceptor. The oxidation of As(III) coincided with the expression of the aoxB gene encoding for the catalytic molybdopterin subunit of the heterodimeric arsenite oxidase operon, indicating the reaction is enzymatically controlled, and M. santoriniensis NKSG1(T) is a heterotrophic As(III)-oxidizing bacterium. Although it is clear that this organism also performs dissimilatory As(V) reduction, no amplification of the arrA arsenate reductase gene was attained using a range of primers and PCR conditions. Marinobacter santoriniensis NKSG1(T) belongs to a genus of bacteria widely occurring in marine environments, including hydrothermal sediments, and is among the first marine bacteria shown to be capable of either anaerobic As(V) respiration or aerobic As(III) oxidation.

journal_name

Environ Microbiol

authors

Handley KM,Héry M,Lloyd JR

doi

10.1111/j.1462-2920.2009.01890.x

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

1601-11

issue

6

eissn

1462-2912

issn

1462-2920

pii

EMI1890

journal_volume

11

pub_type

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