Abstract:
:Thermus thermophilus HB27 is well known for its extraordinary trait of high frequencies of natural transformation, which is considered a major mechanism of horizontal gene transfer. We show that the DNA translocator of T. thermophilus binds and transports DNA from members of all three domains. These results, together with the data obtained from genome comparisons, suggest that the DNA translocator of T. thermophilus has a major impact in adaptation of Thermus to thermal stress conditions and interdomain DNA transfer in extreme hot environments. DNA transport in T. thermophilus is mediated by a macromolecular transport machinery that consists of at least 16 subunits and spans the cytoplasmic membrane and the entire cell periphery. Here, we have addressed the role of single subunits in DNA binding and transport. PilQ is involved in DNA binding, ComEA, PilF and PilA4 are involved in transport of DNA through the outer membrane and PilM, PilN, PilO, PilA1-3, PilC and ComEC are essential for the transport of DNA through the thick cell wall layers and/or through the inner membrane. These data are discussed in the light of the subcellular localization of the proteins. A topological model for DNA transport across the cell wall is presented.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Schwarzenlander C,Haase W,Averhoff Bdoi
10.1111/j.1462-2920.2008.01801.xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
801-8issue
4eissn
1462-2912issn
1462-2920journal_volume
11pub_type
杂志文章abstract::Nitrous oxide (N(2)O) emission from soils is a major contributor to the atmospheric loading of this potent greenhouse gas. It is thought that autotrophic ammonia oxidizing bacteria (AOB) are a significant source of soil-derived N(2)O and a denitrification pathway (i.e. reduction of NO(2) (-) to NO and N(2)O), so-calle...
journal_title:Environmental microbiology
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2010.02178.x
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pub_type: 杂志文章
doi:10.1111/1462-2920.12866
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2011.02547.x
更新日期:2011-10-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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journal_title:Environmental microbiology
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12895
更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/1462-2920.12888
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13054
更新日期:2016-10-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12900
更新日期:2015-08-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2004.00643.x
更新日期:2004-09-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2005.00824.x
更新日期:2005-09-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15048
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/1462-2920.14370
更新日期:2019-01-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12213
更新日期:2014-04-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2012.02744.x
更新日期:2012-08-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14264
更新日期:2018-06-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2010.02277.x
更新日期:2010-11-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12335
更新日期:2014-10-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01608.x
更新日期:2008-07-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15140
更新日期:2020-09-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2001.00155.x
更新日期:2001-01-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12903
更新日期:2015-11-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.01907.x
更新日期:2009-07-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12469
更新日期:2014-07-01 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15350
更新日期:2020-12-07 00:00:00
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14043
更新日期:2018-03-01 00:00:00