Transcriptional response of the obligate anaerobe Desulfuribacillus stibiiarsenatis MLFW-2T to growth on antimonate and other terminal electron acceptors.

Abstract:

:Enzymes of the dimethyl sulfoxide reductase (DMSOR) family catalyse two-electron redox reactions pivotal to the dissimilatory metabolism of a variety of organic and inorganic compounds. The draft genome of the obligately anaerobic bacterium Desulfuribacillus stibiiarsenatis MLFW-2T contains 14 genes that are predicted to encode catalytic subunits of DMSOR family enzymes. We quantified transcription of these genes during growth on antimonate, arsenate, nitrate and selenate, with the goal of identifying the respiratory antimonate reductase. Transcription of BHU72_10330, BHU72_03635 and BHU72_07355 was enhanced during growth on arsenate, nitrate and selenate, respectively, implicating these genes as encoding the catalytic subunits of a respiratory arsenate reductase (arrA), periplasmic nitrate reductase (napA) and membrane-bound selenate reductase (srdA) respectively. Transcription of BHU72_07145 increased markedly when MLFW-2T was grown on antimonate, suggesting that this gene encodes the catalytic subunit of a respiratory antimonate reductase, designated anrA. We also compared the transcriptomes of MLFW-2T during growth on antimonate and arsenate to examine the broader physiological response of the organism to growth on these substrates. Relative to arsenate, antimonate was found to induce transcription of genes involved in pathways for dealing with oxidative stress, including those involved in repairing damaged cellular biomolecules and scavenging reactive oxygen species.

journal_name

Environ Microbiol

authors

Abin CA,Hollibaugh JT

doi

10.1111/1462-2920.14503

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

618-630

issue

2

eissn

1462-2912

issn

1462-2920

journal_volume

21

pub_type

杂志文章
  • Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron.

    abstract::Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing condit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15328

    authors: D'Agostino PM,Yeung ACY,Poljak A,David Waite T,Neilan BA

    更新日期:2021-01-01 00:00:00

  • Proton-pumping rhodopsins are abundantly expressed by microbial eukaryotes in a high-Arctic fjord.

    abstract::Proton-pumping rhodopsins provide an alternative pathway to photosynthesis by which solar energy can enter the marine food web. Rhodopsin genes are widely found in marine bacteria, also in the Arctic, and were recently reported from several eukaryotic lineages. So far, little is known about rhodopsin expression in Arc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14035

    authors: Vader A,Laughinghouse HD 4th,Griffiths C,Jakobsen KS,Gabrielsen TM

    更新日期:2018-02-01 00:00:00

  • Proteomics of Colwellia psychrerythraea at subzero temperatures - a life with limited movement, flexible membranes and vital DNA repair.

    abstract::The mechanisms that allow psychrophilic bacteria to remain metabolically active at subzero temperatures result from form and function of their proteins. We present first proteomic evidence of physiological changes of the marine psychrophile Colwellia psychrerythraea 34H (Cp34H) after exposure to subzero temperatures (...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12691

    authors: Nunn BL,Slattery KV,Cameron KA,Timmins-Schiffman E,Junge K

    更新日期:2015-07-01 00:00:00

  • Environmental regulation of the anaerobic oxidation of methane: a comparison of ANME-I and ANME-II communities.

    abstract::The anaerobic oxidation of methane (AOM) is one of the major sinks for methane on earth and is known to be mediated by at least two phylogenetically different groups of anaerobic methanotrophic Archaea (ANME-I and ANME-II). We present the first comparative in vitro study of the environmental regulation and physiology ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00669.x

    authors: Nauhaus K,Treude T,Boetius A,Krüger M

    更新日期:2005-01-01 00:00:00

  • Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen.

    abstract::Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86 kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal ins...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13197

    authors: Wang J,Ying SH,Hu Y,Feng MG

    更新日期:2016-03-01 00:00:00

  • Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern.

    abstract::Bacterial and archaeal assemblages have been studied in a multipond solar saltern using a range of microbial ecology techniques by four laboratories simultaneously. These include 16S rDNA sequencing from both denaturing gradient gel electrophoresis (DGGE) and clone libraries, and culturing methods. Water samples from ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00306.x

    authors: Benlloch S,López-López A,Casamayor EO,Øvreås L,Goddard V,Daae FL,Smerdon G,Massana R,Joint I,Thingstad F,Pedrós-Alió C,Rodríguez-Valera F

    更新日期:2002-06-01 00:00:00

  • Evidence of distinct pathways for bacterial degradation of the steroid compound cholate suggests the potential for metabolic interactions by interspecies cross-feeding.

    abstract::The distribution and the metabolic pathways of bacteria degrading steroid compounds released by eukaryotic organisms were investigated using the bile salt cholate as model substrate. Cholate-degrading bacteria could be readily isolated from freshwater environments. All isolated strains transiently released steroid deg...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12407

    authors: Holert J,Yücel O,Suvekbala V,Kulić Z,Möller H,Philipp B

    更新日期:2014-05-01 00:00:00

  • Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells.

    abstract::The molecular mechanisms behind the mutagenic effect of reactive oxygen species (ROS) released by defective metabolization of xenobiotic 2,4-dinitrotoluene (DNT) by a still-evolving degradation pathway were studied. To this end, the genes required for biodegradation of DNT from Burkholderia cepacia R34 were implanted ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14459

    authors: Akkaya Ö,Nikel PI,Pérez-Pantoja D,de Lorenzo V

    更新日期:2019-01-01 00:00:00

  • Large-scale distribution and activity patterns of an extremely low-light-adapted population of green sulfur bacteria in the Black Sea.

    abstract::The Black Sea chemocline represents the largest extant habitat of anoxygenic phototrophic bacteria and harbours a monospecific population of Chlorobium phylotype BS-1. High-sensitivity measurements of underwater irradiance and sulfide revealed that the optical properties of the overlying water column were similar acro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02178.x

    authors: Marschall E,Jogler M,Hessge U,Overmann J

    更新日期:2010-05-01 00:00:00

  • Aerobic methane oxidation and methanotroph community composition during seasonal stratification in Mono Lake, California (USA).

    abstract::Patterns of aerobic methane (CH4) oxidation and associated methanotroph community composition were investigated during the development of seasonal stratification in Mono Lake, California (USA). CH4 oxidation rates were measured using a tritiated CH4 radiotracer technique. Fluorescence in situ hybridization (FISH), den...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00786.x

    authors: Carini S,Bano N,LeCleir G,Joye SB

    更新日期:2005-08-01 00:00:00

  • In situ survey of life cycle phases of the coccolithophore Emiliania huxleyi (Haptophyta).

    abstract::The cosmopolitan coccolithophore Emiliania huxleyi is characterized by a strongly differentiated haplodiplontic life cycle consisting of a diploid phase, generally bearing coccoliths (calcified) but that can be also non-calcified, and a non-calcified biflagellated haploid phase. Given most studies have focused on the ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02745.x

    authors: Frada MJ,Bidle KD,Probert I,de Vargas C

    更新日期:2012-06-01 00:00:00

  • Identification accuracy and diversity reproducibility associated with internal transcribed spacer-based fungal taxonomic library preparation.

    abstract::This study investigated analytical parameters that are inherently relevant to identifying and quantifying fungal communities based on polymerase chain reaction amplicons. Specifically, we evaluated the accuracy of the BLASTn-based identification for internal transcribed spacer (ITS) sequences generated from pure cultu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12338

    authors: Yamamoto N,Dannemiller KC,Bibby K,Peccia J

    更新日期:2014-09-01 00:00:00

  • Fungal volatiles mediate cheese rind microbiome assembly.

    abstract::In vitro studies in plant, soil, and human systems have shown that microbial volatiles can mediate microbe-microbe or microbe-host interactions. These previous studies have often used artificially high concentrations of volatiles compared to in situ systems and have not demonstrated the roles volatiles play in mediati...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15223

    authors: Cosetta CM,Kfoury N,Robbat A,Wolfe BE

    更新日期:2020-11-01 00:00:00

  • Songbirds as general transmitters but selective amplifiers of Borrelia burgdorferi sensu lato genotypes in Ixodes rinicus ticks.

    abstract::We investigated to what extent a European songbird (Parus major) selectively transmits and amplifies Borrelia burgdorferi s.l. bacteria. Borrelia-naïve birds were recurrently exposed to Ixodes ricinus nymphs carrying a community of more than 34 5S-23S genotypes belonging to five genospecies (Borrelia garinii, Borrelia...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12304

    authors: Heylen D,Matthysen E,Fonville M,Sprong H

    更新日期:2014-09-01 00:00:00

  • Variation at the common polysaccharide antigen locus drives lipopolysaccharide diversity within the Pseudomonas syringae species complex.

    abstract::The common polysaccharide antigen (CPA) of the lipopolysaccharide (LPS) from Pseudomonas syringae is highly variable, but the genetic basis for this is poorly understood. We have characterized the CPA locus from P. syringae pv. actinidiae (Psa). This locus has genes for l- and d-rhamnose biosynthesis and an operon cod...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15250

    authors: Jayaraman J,Jones WT,Harvey D,Hemara LM,McCann HC,Yoon M,Warring SL,Fineran PC,Mesarich CH,Templeton MD

    更新日期:2020-12-01 00:00:00

  • The FloR master regulator controls flotation, virulence and antibiotic production in Serratia sp. ATCC 39006.

    abstract::Serratia sp. ATCC 39006 produces intracellular gas vesicles to enable upward flotation in water columns. It also uses flagellar rotation to swim through liquid and swarm across semi-solid surfaces. Flotation and motility can be co-regulated with production of a β-lactam antibiotic (carbapenem carboxylate) and a linear...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15048

    authors: Quintero-Yanes A,Lee CM,Monson R,Salmond G

    更新日期:2020-07-01 00:00:00

  • Two Pseudomonas aeruginosa clonal groups belonging to the PA14 clade are indigenous to the Churince system in Cuatro Ciénegas Coahuila, México.

    abstract::Pseudomonas aeruginosa is a widely distributed environmental bacterium but is also an opportunistic pathogen that represents an important health hazard due to its high intrinsic antibiotic resistance and its production of virulence factors. The genetic structure of P. aeruginosa populations using whole genome sequence...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14692

    authors: García-Ulloa M 2nd,Ponce-Soto GY,González-Valdez A,González-Pedrajo B,Díaz-Guerrero M,Souza V,Soberón-Chávez G

    更新日期:2019-08-01 00:00:00

  • Off-axis symbiosis found: Characterization and biogeography of bacterial symbionts of Bathymodiolus mussels from Lost City hydrothermal vents.

    abstract::Organisms at hydrothermal vents inhabit discontinuous chemical 'islands' along mid-ocean ridges, a scenario that may promote genetic divergence among populations. The 2003 discovery of mussels at the Lost City Hydrothermal Field provided a means of evaluating factors that govern the biogeography of symbiotic bacteria ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.01113.x

    authors: DeChaine EG,Bates AE,Shank TM,Cavanaugh CM

    更新日期:2006-11-01 00:00:00

  • Methanogenic toluene metabolism: community structure and intermediates.

    abstract::Toluene is a model compound used to study the anaerobic biotransformation of aromatic hydrocarbons. Reports indicate that toluene is transformed via fumarate addition to form benzylsuccinate or by unknown mechanisms to form hydroxylated intermediates under methanogenic conditions. We investigated the mechanism(s) of s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02631.x

    authors: Fowler SJ,Dong X,Sensen CW,Suflita JM,Gieg LM

    更新日期:2012-03-01 00:00:00

  • Exploring bacterial pathogen community dynamics in freshwater beach sediments: A tale of two lakes.

    abstract::Pathogenic bacteria associated with freshwater ecosystems can pose significant health risks particularly where recreational water use is popular. Common water quality assessments involve quantifying indicator Escherichia coli within the water column but neglect to consider physical and geochemical factors and contribu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14860

    authors: VanMensel D,Chaganti SR,Droppo IG,Weisener CG

    更新日期:2020-02-01 00:00:00

  • Diversity patterns of Rhizobiaceae communities inhabiting soils, root surfaces and nodules reveal a strong selection of rhizobial partners by legumes.

    abstract::Current knowledge about rhizobial diversity patterns in non-nodule habitats is scarce, limiting our understanding of basic aspects of rhizobial ecology like competitiveness for nodule occupancy and host effects on community structure. We used a combination of cultivation-dependent and independent approaches to analyse...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13061

    authors: Miranda-Sánchez F,Rivera J,Vinuesa P

    更新日期:2016-09-01 00:00:00

  • Campylobacter lari: genotype and antibiotic resistance of isolates from cattle, wildlife and water in an area of mixed dairy farmland in the United Kingdom.

    abstract::Campylobacter lari is a rare human pathogen most commonly associated with birds and shellfish. Little information has been published regarding its prevalence in other environments, or on its potential role as a reservoir of antibiotic resistance. In this study, we characterized 109 C. lari isolated from a range of hos...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01295.x

    authors: Leatherbarrow AJ,Griffiths R,Hart CA,Kemp R,Williams NJ,Diggle PJ,Wright EJ,Sutherst J,Houghton P,French NP

    更新日期:2007-07-01 00:00:00

  • The tail-associated depolymerase of Erwinia amylovora phage L1 mediates host cell adsorption and enzymatic capsule removal, which can enhance infection by other phage.

    abstract::The depolymerase enzyme (DpoL1) encoded by the T7-like phage L1 efficiently degrades amylovoran, an important virulence factor and major component of the extracellular polysaccharide (EPS) of its host, the plant pathogen Erwinia amylovora. Mass spectrometry analysis of hydrolysed EPS revealed that DpoL1 cleaves the ga...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12212

    authors: Born Y,Fieseler L,Klumpp J,Eugster MR,Zurfluh K,Duffy B,Loessner MJ

    更新日期:2014-07-01 00:00:00

  • Proteomic assessment of host-associated microevolution in the fungus Thielaviopsis basicola.

    abstract::Thielaviopsis basicola, a soil-borne pathogen with a broad host range and a cosmopolitan distribution, is emerging as a major risk to sustainable cotton production in Australia. Previous studies suggested that host specialization has occurred making T. basicola an ideal model for a comparative proteomic analysis of st...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02358.x

    authors: Coumans JV,Harvey J,Backhouse D,Poljak A,Raftery MJ,Nehl D,Katz ME,Pereg L

    更新日期:2011-03-01 00:00:00

  • Molecular diversity among marine picophytoplankton as revealed by psbA analyses.

    abstract::Photosynthetic microorganisms play a crucial role in the marine environment. In vast areas of the oceans, marine primary productivity is performed by cells smaller than 2-3 micro m (picoplankton). Here, we report on molecular analyses of the conserved photosynthetic psbA gene (coding for protein D1 of photosystem II r...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2003.00403.x

    authors: Zeidner G,Preston CM,Delong EF,Massana R,Post AF,Scanlan DJ,Béjà O

    更新日期:2003-03-01 00:00:00

  • Structure and function of methanogenic microbial communities in sediments of Amazonian lakes with different water types.

    abstract::Tropical lake sediments are a significant source for the greenhouse gas methane. We studied function (pathway, rate) and structure (abundance, taxonomic composition) of the microbial communities (Bacteria, Archaea) leading to methane formation together with the main physicochemical characteristics in the sediments of ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13491

    authors: Ji Y,Angel R,Klose M,Claus P,Marotta H,Pinho L,Enrich-Prast A,Conrad R

    更新日期:2016-12-01 00:00:00

  • Near-zero growth kinetics of Pseudomonas putida deduced from proteomic analysis.

    abstract::Intensive microbial growth typically observed in laboratory rarely occurs in nature. Because of severe nutrient deficiency, natural populations exhibit near-zero growth (NZG). There is a long-standing controversy about sustained NZG, specifically whether there is a minimum growth rate below which cells die or whether ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12584

    authors: Panikov NS,Mandalakis M,Dai S,Mulcahy LR,Fowle W,Garrett WS,Karger BL

    更新日期:2015-01-01 00:00:00

  • Sequencing effort dictates gene discovery in marine microbial metagenomes.

    abstract::Massive metagenomic sequencing combined with gene prediction methods were previously used to compile the gene catalogue of the ocean and host-associated microbes. Global expeditions conducted over the past 15 years have sampled the ocean to build a catalogue of genes from pelagic microbes. Here we undertook a large se...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15182

    authors: Duarte CM,Ngugi DK,Alam I,Pearman J,Kamau A,Eguiluz VM,Gojobori T,Acinas SG,Gasol JM,Bajic V,Irigoien X

    更新日期:2020-11-01 00:00:00

  • Involvement of the ABC transporter BcAtrB and the laccase BcLCC2 in defence of Botrytis cinerea against the broad-spectrum antibiotic 2,4-diacetylphloroglucinol.

    abstract::The genetic and biochemical basis of defence mechanisms in plant pathogenic fungi against antifungal compounds produced by antagonistic microorganisms is largely unknown. The results of this study show that both degradative and non-degradative defence mechanisms enable the plant pathogenic fungus Botrytis cinerea to r...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01531.x

    authors: Schouten A,Maksimova O,Cuesta-Arenas Y,van den Berg G,Raaijmakers JM

    更新日期:2008-05-01 00:00:00

  • Molecular diversity of methanotrophs in Transbaikal soda lake sediments and identification of potentially active populations by stable isotope probing.

    abstract::Soda lakes are an environment with an unusually high pH and often high salinity. To identify the active methanotrophs in the Soda lake sediments, sediment slurries were incubated with a 10% (v/v) (13)CH(4) headspace and the (13)C-labelled DNA was subsequently extracted from these sediments following CsCl density gradi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00635.x

    authors: Lin JL,Radajewski S,Eshinimaev BT,Trotsenko YA,McDonald IR,Murrell JC

    更新日期:2004-10-01 00:00:00