Abstract:
:Bacterial degradation of polycyclic aromatic hydrocarbons (PAHs), ubiquitous contaminants from oil and coal, is typically limited by poor accessibility of the contaminant to the bacteria. In order to measure PAH availability in complex systems, we designed a number of diffusion-based assays with a double-tagged bacterial reporter strain Burkholderia sartisoli RP037-mChe. The reporter strain is capable of mineralizing phenanthrene (PHE) and induces the expression of enhanced green fluorescent protein (eGFP) as a function of the PAH flux to the cell. At the same time, it produces a second autofluorescent protein (mCherry) in constitutive manner. Quantitative epifluorescence imaging was deployed in order to record reporter signals as a function of PAH availability. The reporter strain expressed eGFP proportionally to dosages of naphthalene or PHE in batch liquid cultures. To detect PAH diffusion from solid materials the reporter cells were embedded in 2 cm-sized agarose gel patches, and fluorescence was recorded over time for both markers as a function of distance to the PAH source. eGFP fluorescence gradients measured on known amounts of naphthalene or PHE served as calibration for quantifying PAH availability from contaminated soils. To detect reporter gene expression at even smaller diffusion distances, we mixed and immobilized cells with contaminated soils in an agarose gel. eGFP fluorescence measurements confirmed gel patch diffusion results that exposure to 2-3 mg lampblack soil gave four times higher expression than to material contaminated with 10 or 1 (mg PHE) g(-1).
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Tecon R,Binggeli O,van der Meer JRdoi
10.1111/j.1462-2920.2009.01952.xsubject
Has Abstractpub_date
2009-09-01 00:00:00pages
2271-83issue
9eissn
1462-2912issn
1462-2920pii
EMI1952journal_volume
11pub_type
杂志文章abstract::Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86 kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal ins...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13197
更新日期:2016-03-01 00:00:00
abstract::Microbes perform many costly biological functions that benefit themselves, and may also benefit neighbouring cells. Losing the ability to perform such functions can be advantageous due to cost savings, but when they are essential for growth, organisms become dependent on ecological partners to compensate for those los...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13028
更新日期:2016-05-01 00:00:00
abstract::The microbiota that colonizes the human intestinal tract is complex and its structure is specific for each of us. In this study we expand the knowledge about the stability of the subject-specific microbiota and show that this ecosystem is stable in short-term intervals (< 1 year) but also during long periods of time (...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12023
更新日期:2012-10-15 00:00:00
abstract::Methylotrophy is a metabolic capability possessed by microorganisms that allows them to build biomass and to obtain energy from organic substrates containing no carbon-carbon bonds (C1 compounds, such as methane, methanol, etc.). This phenomenon in microbial physiology has been a subject of study for over 100 years, e...
journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1462-2920.2011.02464.x
更新日期:2011-10-01 00:00:00
abstract::The sulfate-reducing culture N47 can utilize naphthalene or 2-methylnaphthalene as the sole carbon source and electron donor. Here we show that the initial reaction in the naphthalene degradation pathway is a methylation to 2-methylnaphthalene which then undergoes the subsequent oxidation to the central metabolite 2-n...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2005.00900.x
更新日期:2006-02-01 00:00:00
abstract::The deep sea is a unique and extreme environment characterized by low concentrations of highly recalcitrant carbon. As a consequence, large organic inputs have potential to cause significant perturbation. To assess the impact of organic enrichment on deep sea microbial communities, we investigated bacterial diversity ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.02072.x
更新日期:2010-02-01 00:00:00
abstract::The annual dynamics of three different ammonia-oxidizing archaea (AOA) ecotypes (amoA gene) and of the SAGMGC-1 (Nitrosotalea-like aquatic Thaumarchaeota) group (16S rRNA gene) were studied by newly designed specific primers and quantitative polymerase chain reaction analysis in a deep oligotrophic high mountain lake ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12191
更新日期:2014-03-01 00:00:00
abstract::The green sulfur bacteria, the Chlorobi, are phototrophic bacteria that oxidize sulfide and deposit extracellular elemental sulfur globules [S(0)]. These are subsequently consumed after sulfide is exhausted. S(0) globules from a Chlorobaculum tepidum mutant strain were purified and used to show that the wild-type stra...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12995
更新日期:2016-09-01 00:00:00
abstract::The mechanisms that allow psychrophilic bacteria to remain metabolically active at subzero temperatures result from form and function of their proteins. We present first proteomic evidence of physiological changes of the marine psychrophile Colwellia psychrerythraea 34H (Cp34H) after exposure to subzero temperatures (...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12691
更新日期:2015-07-01 00:00:00
abstract::Previous studies have shown that the rate of settlement of zoospores of the green alga Enteromorpha is stimulated by mixed microbial biofilms and that the number of zoospores settling is positively correlated with the number of bacteria in the biofilm. In the present study the specificity of this relationship has been...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2003.00407.x
更新日期:2003-05-01 00:00:00
abstract::Environmental heat stress impacts on the physiology and viability of microbial cells with concomitant implications for microbial activity and diversity. Previously, it has been demonstrated that gradual heating of Saccharomyces cerevisiae induces a degree of thermal resistance, whereas a heat shock results in a high l...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12866
更新日期:2015-08-01 00:00:00
abstract::Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important disease in Canada. The worldwide genetic structure of Pst populations have been characterized, excluding Canada. Here, we elucidated the genetic structure of the western Canadian Pst population using molecular markers, revealing th...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14067
更新日期:2018-04-01 00:00:00
abstract::We studied the vanA-carrying vancomycin-resistant enterococci (VRE) isolated from American crows in the United States during the winter 2011/2012. Faecal samples from crows were cultured selectively for VRE and characterized. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to ex...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12213
更新日期:2014-04-01 00:00:00
abstract::Cyanobacteria were the first organisms ever to perform oxygenic photosynthesis and still significantly contribute to primary production on a global scale. To assure the proper functioning of their primary metabolism and cell homeostasis, cyanobacteria must rely on efficient transport systems to cross their multilayere...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14095
更新日期:2018-07-01 00:00:00
abstract::There has been a growing interest in the degradation of 2,4,6-trinitrotoluene (TNT) over the last decade, ever since its removal from polluted sites was declared an international environmental priority. Certain aerobic and anaerobic microorganisms are capable of using TNT as an N source, although very few studies have...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2007.01272.x
更新日期:2007-06-01 00:00:00
abstract::A recent analysis revealed that most environmental microbiologists neglect replication in their science (Prosser, 2010). Of all peer-reviewed papers published during 2009 in the field's leading journals, slightly more than 70% lacked replication when it came to analyzing microbial community data. The paucity of replic...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2011.02445.x
更新日期:2011-06-01 00:00:00
abstract::Endophytic microorganisms asymptomatically colonise plant tissues. Exploring the assembly dynamics of bacterial endophytic communities is essential to understand the functioning of the plant holobiont and to optimise their possible use as biopesticides or plant biostimulants. The variation in endophytic communities in...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13843
更新日期:2017-08-01 00:00:00
abstract::In a future scenario of increasing temperatures in North-Atlantic waters, the risk associated with the expansion of the harmful, benthic dinoflagellate Ostreopsis cf. siamensis has to be evaluated and monitored. Microscopy observations and spatio-temporal surveys of environmental DNA (eDNA) were associated with Lagran...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15406
更新日期:2021-01-26 00:00:00
abstract::Harmful blooms of the cyanobacterium Microcystis sp. have become increasingly pervasive in the San Francisco Estuary Delta (USA) since the early 2000s and their rise has coincided with substantial decreases in several important fish species. Direct and indirect effects Microcystis blooms may have on the Delta food web...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13860
更新日期:2017-09-01 00:00:00
abstract::Phytoplankton influence the composition of bacterial communities, but the taxonomic specificity of algal-bacterial interactions is unclear due to the aggregation of ecologically distinct bacterial populations by community characterization methods. Here we examine whether phytoplankton seasonal succession affects the c...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12529
更新日期:2015-03-01 00:00:00
abstract::Marine Crenarchaeota represent an abundant component of the oceanic microbiota that play an important role in the global nitrogen cycle. Here we report the association of the colonial ascidian Cystodytes dellechiajei with putative ammonia-oxidizing Crenarchaeota that could actively be involved in nitrification inside ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01761.x
更新日期:2008-11-01 00:00:00
abstract::Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that infects immunocompromised patients and trauma victims and causes fatal lung infections in people with cystic fibrosis. This microorganism produces a number of virulence factors, one of which is lipopolysaccharide (LPS), which has been shown to media...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2002.00288.x
更新日期:2002-12-01 00:00:00
abstract::Pseudomonas fluorescens CHA0 is a root-associated biocontrol agent that suppresses soil-borne fungal diseases of crops. Remarkably, the pseudomonad is also endowed with systemic and oral activity against pest insects which depends on the production of the insecticidal Fit toxin. The toxin gene (fitD) is part of a viru...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12050
更新日期:2013-03-01 00:00:00
abstract::Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling. Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to eva...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14304
更新日期:2018-08-01 00:00:00
abstract::Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14297
更新日期:2018-08-01 00:00:00
abstract::Lignocellulose degradation is central to the carbon cycle and renewable biotechnologies. The xyloglucan (XyG), β(1→3)/β(1→4) mixed-linkage glucan (MLG) and β(1→3) glucan components of lignocellulose represent significant carbohydrate energy sources for saprophytic microorganisms. The bacterium Cellvibrio japonicus has...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13959
更新日期:2017-12-01 00:00:00
abstract::Reef-building corals are comprised of close associations between the coral animal, symbiotic zooxanthellae, and a diversity of associated microbes (including Bacteria, Archaea and Fungi). Together, these comprise the coral holobiont - a paradigm that emphasizes the potential contributions of each component to the over...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.02027.x
更新日期:2010-01-01 00:00:00
abstract::Homeostatic control of nitric oxide (NO) at nanomolar concentrations appears common among denitrifying bacteria, often ascribed to synchronized expression of nitrite and nitric oxide reductase (Nir and Nor). We questioned whether this is sufficient: using the reported substrate affinities for cytochrome cd1 nitrite re...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13129
更新日期:2016-09-01 00:00:00
abstract::Tropical lake sediments are a significant source for the greenhouse gas methane. We studied function (pathway, rate) and structure (abundance, taxonomic composition) of the microbial communities (Bacteria, Archaea) leading to methane formation together with the main physicochemical characteristics in the sediments of ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13491
更新日期:2016-12-01 00:00:00
abstract::In vitro studies in plant, soil, and human systems have shown that microbial volatiles can mediate microbe-microbe or microbe-host interactions. These previous studies have often used artificially high concentrations of volatiles compared to in situ systems and have not demonstrated the roles volatiles play in mediati...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15223
更新日期:2020-11-01 00:00:00