Abstract:
:The cattle tick Rhipicephalus (Boophilus) microplus lays eggs in the soil near the roots of grass, or in similar highly moist environments that are prone to biofilm formation. Tick eggs have a protective wax coating that may be a source of nutrients for microorganisms. However, as the eggs remain viable and show no visible signs of microbial colonization, we hypothesized that the coating might have anti-biofilm properties. We show here that the coating inhibits biofilm formation by both Gram-negative and Gram-positive bacteria, though by different mechanisms. We have identified the anti-biofilm molecule as N-(3-sulfooxy-25-cholest-5-en-26-oyl)-L-isoleucine (boophiline), and we show that it inhibits the expression of fliC (flagellin) and cdrA (biofilm scaffold), whose products are necessary for biofilm formation in Pseudomonas aeruginosa. Boophiline is a novel biofilm inhibitor being also effective against Staphylococcus epidermidis biofilm. In our study we show evidences of the boophiline mode of action in the protection of arthropod eggs against biofilm colonization.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Zimmer KR,Macedo AJ,Giordani RB,Conceição JM,Nicastro GG,Boechat AL,Baldini RL,Abraham WR,Termignoni Cdoi
10.1111/1462-2920.12082subject
Has Abstractpub_date
2013-07-01 00:00:00pages
2008-18issue
7eissn
1462-2912issn
1462-2920journal_volume
15pub_type
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