An alpha-amylase is a novel receptor for Bacillus thuringiensis ssp. israelensis Cry4Ba and Cry11Aa toxins in the malaria vector mosquito Anopheles albimanus (Diptera: Culicidae).

Abstract:

:Bacillus thuringiensis ssp. israelensis (Bti) produces four Cry toxins (Cry4Aa, Cry4Ba, Cry10Aa and Cry11Aa), and two Cyt proteins (Cyt1Aa and Cyt2Ba), toxic to mosquito-larvae of the genus Aedes, Anopheles and Culex, important human disease vectors that transmit dengue virus, malaria and filarial parasites respectively. Previous work showed that Bti is highly toxic to Anopheles albimanus, the main vector for transmission of malaria in Mexico. In this work, we analysed the toxicity of isolated Cry proteins of Bti and identified an An. albimanus midgut protein as a putative Cry4Ba and Cry11Aa receptor molecule. Biossays showed that Cry4Ba and Cry11Aa of Bti are toxic to An. albimanus larvae. Ligand blot assays indicated that a 70 kDa glycosylphosphatidylinositol-anchored protein present in midgut brush border membrane vesicles of An. albimanus interacts with Cry4Ba and Cry11Aa toxins. This protein was identified as an alpha-amylase by mass spectrometry and enzymatic activity assays. The cDNA that codes for the alpha-amylase was cloned by means of 5'- and 3'-RACE experiments. Recombinant alpha-amylase expressed in Escherichia coli specifically binds Cry4Ba and Cry11Aa toxins.

journal_name

Environ Microbiol

authors

Fernandez-Luna MT,Lanz-Mendoza H,Gill SS,Bravo A,Soberon M,Miranda-Rios J

doi

10.1111/j.1462-2920.2009.02117.x

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

746-57

issue

3

eissn

1462-2912

issn

1462-2920

pii

EMI2117

journal_volume

12

pub_type

杂志文章
  • Phylogenetic identification and metabolism of marine dimethylsulfide-consuming bacteria.

    abstract::Microbial consumption is one of the main processes, along with photolysis and ventilation, that remove the biogenic trace gas dimethylsulfide (DMS) from the surface ocean. Although a few isolates of marine bacteria have been studied for their ability to utilize DMS, little is known about the characteristics or phyloge...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01102.x

    authors: Vila-Costa M,Del Valle DA,González JM,Slezak D,Kiene RP,Sánchez O,Simó R

    更新日期:2006-12-01 00:00:00

  • Comparison of coastal phytoplankton composition estimated from the V4 and V9 regions of the 18S rRNA gene with a focus on photosynthetic groups and especially Chlorophyta.

    abstract::We compared the composition of eukaryotic communities using two genetic markers (18S rRNA V4 and V9 regions) at 27 sites sampled during Ocean Sampling Day 2014, with a focus on photosynthetic groups and, more specifically green algae (Chlorophyta). Globally, the V4 and V9 regions of the 18S rRNA gene provided similar ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13952

    authors: Tragin M,Zingone A,Vaulot D

    更新日期:2018-02-01 00:00:00

  • Assessing sub-seafloor microbial activity by combined stable isotope probing with deuterated water and 13C-bicarbonate.

    abstract::Sub-seafloor sediments are populated by large numbers of microbial cells but not much is known about their metabolic activities, growth rates and carbon assimilation pathways. Here we introduce a new method enabling the sensitive detection of microbial lipid production and the distinction of auto- and heterotrophic ca...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02739.x

    authors: Wegener G,Bausch M,Holler T,Thang NM,Prieto Mollar X,Kellermann MY,Hinrichs KU,Boetius A

    更新日期:2012-06-01 00:00:00

  • Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields.

    abstract::The stimulation of bacteria capable of reducing soluble U(VI) to sparingly soluble U(IV) is a promising approach for containing U(VI) plumes. Anaeromyxobacter dehalogenans is capable of mediating this activity; however, its ability to couple U(VI) reduction to growth has not been established. Monitoring the increase i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01405.x

    authors: Sanford RA,Wu Q,Sung Y,Thomas SH,Amos BK,Prince EK,Löffler FE

    更新日期:2007-11-01 00:00:00

  • Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.

    abstract::During the anaerobic biodegradation of naphthalene and methylnaphthalene, unique metabolites are formed by specific microbial carboxylation and ring-reduction reactions. Groundwater samples from an anoxic, shallow aquifer contaminated with gasoline were examined for the presence of these metabolites by extraction, der...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00330.x

    authors: Phelps CD,Battistelli J,Young LY

    更新日期:2002-09-01 00:00:00

  • Disruption of quorum sensing in seawater abolishes attraction of zoospores of the green alga Ulva to bacterial biofilms.

    abstract::Zoospores of the eukaryotic green seaweed Ulva respond to bacterial N-acylhomoserine lactone (AHL) quorum sensing signal molecules for the selection of surface sites for permanent attachment. In this study we have investigated the production and destruction of AHLs in biofilms of the AHL-producing marine bacterium, Vi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00706.x

    authors: Tait K,Joint I,Daykin M,Milton DL,Williams P,Cámara M

    更新日期:2005-02-01 00:00:00

  • Inorganic carbon fixation by sulfate-reducing bacteria in the Black Sea water column.

    abstract::The Black Sea is the largest anoxic water basin on Earth and its stratified water column comprises an upper oxic, middle suboxic and a lower permanently anoxic, sulfidic zone. The abundance of sulfate-reducing bacteria (SRB) in water samples was determined by quantifying the copy number of the dsrA gene coding for the...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01413.x

    authors: Neretin LN,Abed RM,Schippers A,Schubert CJ,Kohls K,Kuypers MM

    更新日期:2007-12-01 00:00:00

  • Evidence for selective bacterial community structuring on microplastics.

    abstract::In aquatic ecosystems, microplastics are a relatively new anthropogenic substrate that can readily be colonized by biofilm-forming organisms. To examine the effects of substrate type on microbial community assembly, we exposed ambient Baltic bacterioplankton to plastic substrates commonly found in marine environments ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14120

    authors: Ogonowski M,Motiei A,Ininbergs K,Hell E,Gerdes Z,Udekwu KI,Bacsik Z,Gorokhova E

    更新日期:2018-08-01 00:00:00

  • Sequential bioavailability of sedimentary organic matter to heterotrophic bacteria.

    abstract::Aquatic sediments harbour diverse microbial communities that mediate organic matter degradation and influence biogeochemical cycles. The pool of bioavailable carbon continuously changes as a result of abiotic processes and microbial activity. It remains unclear how microbial communities respond to heterogeneous organi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13745

    authors: Mahmoudi N,Beaupré SR,Steen AD,Pearson A

    更新日期:2017-07-01 00:00:00

  • Nitrogen fixation by a marine non-heterocystous cyanobacterium requires a heterotrophic bacterial consort.

    abstract::Cultures of the non-heterocystous cyanobacterium, Leptolyngbya nodulosa, could be grown indefinitely in media devoid of combined nitrogen. Acetylene reduction assays showed that these cultures fixed nitrogen in the dark period of a diurnal cycle under micro-oxygenic or anaerobic conditions. Addition of DCMU to culture...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02159.x

    authors: Li Z,Yu J,Kim KR,Brand J

    更新日期:2010-05-01 00:00:00

  • High phenotypic diversity in infecting but not in colonizing Staphylococcus aureus populations.

    abstract::In hostile environments diversity within a bacterial population may be beneficial for the fitness of the microbial community as a whole. Here we analysed the population diversity of Staphylococcus aureus in infecting and colonizing situations. In the study, performed independently in two German centres, the heterogene...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01423.x

    authors: Goerke C,Gressinger M,Endler K,Breitkopf C,Wardecki K,Stern M,Wolz C,Kahl BC

    更新日期:2007-12-01 00:00:00

  • Analysis and comparison of the microbial community structures of two enrichment cultures capable of reductively dechlorinating TCE and cis-DCE.

    abstract::In order to study the effect of different chloroethenes (electron acceptors) on the bacterial composition of dechlorinating communities, two reductive dechlorinating enrichment cultures were developed that were able to reduce trichloroethene (TCE) and cis-1,2-dichloroethene (cis-DCE) to ethene using hydrogen as electr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2003.00525.x

    authors: Gu AZ,Hedlund BP,Staley JT,Strand SE,Stensel HD

    更新日期:2004-01-01 00:00:00

  • Susceptibility of Vibrio aestuarianus 01/032 to the antibacterial activity of Mytilus haemolymph: identification of a serum opsonin involved in mannose-sensitive interactions.

    abstract::The interactions of Vibrio aestuarianus 01/032 with haemolymph of the bivalves Mytilus galloprovincialis and Crassostrea gigas were investigated to understand if haemolymph components (haemocytes and soluble factors) could be involved in the higher resistance to microbial infection shown by mussels in comparison with ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12750

    authors: Pezzati E,Canesi L,Damonte G,Salis A,Marsano F,Grande C,Vezzulli L,Pruzzo C

    更新日期:2015-11-01 00:00:00

  • Bloom of a denitrifying methanotroph, 'Candidatus Methylomirabilis limnetica', in a deep stratified lake.

    abstract::Methanotrophic bacteria represent an important biological filter regulating methane emissions into the atmosphere. Planktonic methanotrophic communities in freshwater lakes are typically dominated by aerobic gamma-proteobacteria, with a contribution from alpha-proteobacterial methanotrophs and the NC10 bacteria. The N...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14285

    authors: Graf JS,Mayr MJ,Marchant HK,Tienken D,Hach PF,Brand A,Schubert CJ,Kuypers MMM,Milucka J

    更新日期:2018-07-01 00:00:00

  • Global physiological analysis of carbon- and energy-limited growing Escherichia coli confirms a high degree of catabolic flexibility and preparedness for mixed substrate utilization.

    abstract::Growth conditions for heterotrophic bacteria in the environment are characterized by low concentrations of carbon and energy sources and complex substrate mixtures. While mechanisms of starvation-survival in the absence of carbon substrates have been studied in considerable detail, information on the physiology of slo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00846.x

    authors: Ihssen J,Egli T

    更新日期:2005-10-01 00:00:00

  • Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage.

    abstract::Cultivation-independent surveys of ribosomal RNA genes have revealed the existence of novel microbial lineages, many with no known cultivated representatives. Ribosomal RNA-based analyses, however, often do not provide significant information beyond phylogenetic affiliation. Analysis of large genome fragments recovere...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00133.x

    authors: Béjà O,Suzuki MT,Koonin EV,Aravind L,Hadd A,Nguyen LP,Villacorta R,Amjadi M,Garrigues C,Jovanovich SB,Feldman RA,DeLong EF

    更新日期:2000-10-01 00:00:00

  • Genome-wide identification of pathogenicity, conidiation and colony sectorization genes in Metarhizium robertsii.

    abstract::Metarhizium robertsii occupies a wide array of ecological niches and has diverse lifestyle options (saprophyte, insect pathogen and plant symbiont), that renders it an unusually effective model for studying genetic mechanisms for fungal adaptation. Here over 20,000 M. robertsii T-DNA mutants were screened in order to ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13777

    authors: Zeng G,Chen X,Zhang X,Zhang Q,Xu C,Mi W,Guo N,Zhao H,You Y,Dryburgh FJ,Bidochka MJ,St Leger RJ,Zhang L,Fang W

    更新日期:2017-10-01 00:00:00

  • Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure.

    abstract::Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis have been described as members of the Bacillus cereus group but are, in fact, one species. B. anthracis is a mammal pathogen, B. thuringiensis an entomopathogen and B. cereus a ubiquitous soil bacterium and an occasional human pathogen. In two clinical iso...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00220.x

    authors: Mignot T,Denis B,Couture-Tosi E,Kolstø AB,Mock M,Fouet A

    更新日期:2001-08-01 00:00:00

  • Cyanobacterial distributions along a physico-chemical gradient in the Northeastern Pacific Ocean.

    abstract::The cyanobacteria Prochlorococcus and Synechococcus are important marine primary producers. We explored their distributions and covariance along a physico-chemical gradient from coastal to open ocean waters in the Northeastern Pacific Ocean. An inter-annual pattern was delineated in the dynamic transition zone where u...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12742

    authors: Sudek S,Everroad RC,Gehman AL,Smith JM,Poirier CL,Chavez FP,Worden AZ

    更新日期:2015-10-01 00:00:00

  • Changes in the community structure and activity of betaproteobacterial ammonia-oxidizing sediment bacteria along a freshwater-marine gradient.

    abstract::To determine whether the distribution of estuarine ammonia-oxidizing bacteria (AOB) was influenced by salinity, the community structure of betaproteobacterial ammonia oxidizers (AOB) was characterized along a salinity gradient in sediments of the Ythan estuary, on the east coast of Scotland, UK, by denaturant gradient...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00947.x

    authors: Freitag TE,Chang L,Prosser JI

    更新日期:2006-04-01 00:00:00

  • Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods.

    abstract::The phylogenetic diversity of Bacteria and Archaea within a biodegraded, mesothermic petroleum reservoir in the Schrader Bluff Formation of Alaska was examined by two culture-independent methods based on fosmid and small-subunit rRNA gene PCR clone libraries. Despite the exclusion of certain groups by each method, the...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01751.x

    authors: Pham VD,Hnatow LL,Zhang S,Fallon RD,Jackson SC,Tomb JF,DeLong EF,Keeler SJ

    更新日期:2009-01-01 00:00:00

  • Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant.

    abstract::The complex ecology of free-living amoebae (FLA) and their role in spreading pathogenic microorganisms through water systems have recently raised considerable interest. In this study, we investigated the presence of FLA and amoebae-resisting bacteria (ARB) at various stages of a drinking water plant fed with river wat...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01693.x

    authors: Thomas V,Loret JF,Jousset M,Greub G

    更新日期:2008-10-01 00:00:00

  • Anaerobic utilization of alkylbenzenes and n-alkanes from crude oil in an enrichment culture of denitrifying bacteria affiliating with the beta-subclass of Proteobacteria.

    abstract::Denitrifying bacteria were enriched from freshwater sediment with added nitrate as electron acceptor and crude oil as the only source of organic substrates. The enrichment cultures were used as laboratory model systems for studying the degradative potential of denitrifying bacteria with respect to crude oil constituen...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.1999.00014.x

    authors: Rabus R,Wilkes H,Schramm A,Harms G,Behrends A,Amann R,Widdel F

    更新日期:1999-04-01 00:00:00

  • Subsistence and complexity of antimicrobial resistance on a community-wide level.

    abstract::There are a multitude of resistance strategies that microbes can apply to avoid inhibition by antimicrobials. One of these strategies is the enzymatic modification of the antibiotic, in a process generally termed inactivation. Furthermore, some microorganisms may not be limited to the mere inactivation of the antimicr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15018

    authors: Perri R,Kolvenbach BA,Corvini PFX

    更新日期:2020-07-01 00:00:00

  • Preferential occurrence of diazotrophic endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with modern races.

    abstract::Several diazotrophic species of Azoarcus spp. occur as endophytes in the pioneer plant Kallar grass. The purpose of this study was to screen Asian wild rice and cultivated Oryza sativa varieties for natural association with these endophytes. Populations of culturable diazotrophs in surface-sterilized roots were charac...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00078.x

    authors: Engelhard M,Hurek T,Reinhold-Hurek B

    更新日期:2000-04-01 00:00:00

  • A periplasmic arsenite-binding protein involved in regulating arsenite oxidation.

    abstract::Arsenic (As) is the most common toxic element in the environment, ranking first on the Superfund List of Hazardous Substances. Microbial redox transformations are the principal drivers of As chemical speciation, which in turn dictates As mobility and toxicity. Consequently, in order to manage or remediate environmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02672.x

    authors: Liu G,Liu M,Kim EH,Maaty WS,Bothner B,Lei B,Rensing C,Wang G,McDermott TR

    更新日期:2012-07-01 00:00:00

  • Spatial scaling of forest soil microbial communities across a temperature gradient.

    abstract::Temperature is an important correlate of global patterns of biodiversity, yet the mechanisms driving these relationships are not well understood. Taxa-area relationships (TARs) have been intensively examined, but the effects of temperature on TARs, particularly for microbial communities, are largely undocumented. Here...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14303

    authors: Deng Y,Ning D,Qin Y,Xue K,Wu L,He Z,Yin H,Liang Y,Buzzard V,Michaletz ST,Zhou J

    更新日期:2018-10-01 00:00:00

  • Distribution of chloropigments in suspended particulate matter and benthic microbial mat of a meromictic lake, Lake Kaiike, Japan.

    abstract::We investigated the distribution of chloropigments in a small meromictic lake, Lake Kaiike, south-west Japan. In the water-column, concentrations of Chl a related to cyanobacteria, BChl a related to purple sulphur bacteria, and three types of BChl e homologues (BChls e1, e2 and e3) related to brown-coloured green sulp...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2003.00517.x

    authors: Nakajima Y,Okada H,Oguri K,Suga H,Kitazato H,Koizumi Y,Fukui M,Ohkouchi N

    更新日期:2003-11-01 00:00:00

  • A novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated 'Brachyspira suanatina' sp. nov.

    abstract::Atypical, strongly haemolytic porcine isolates of intestinal spirochaetes differing genetically from Brachyspira hyodysenteriae were identified and characterized. The isolates were subjected to culture and biochemical tests, antimicrobial susceptibility testing and molecular analyses. None of four species-specific pol...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01220.x

    authors: Råsbäck T,Jansson DS,Johansson KE,Fellström C

    更新日期:2007-04-01 00:00:00

  • High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library.

    abstract::The bacterial diversity of an industrial biofilter used for waste gas abatement in an animal-rendering plant was investigated. A 16S rDNA clone library was generated and 444 clones were screened using computer-aided amplified ribosomal DNA restriction analysis (ARDRA). Of the screened clones, 60.8% showed unique ARDRA...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00349.x

    authors: Friedrich U,Prior K,Altendorf K,Lipski A

    更新日期:2002-11-01 00:00:00