Direct cathodic electron uptake coupled to sulfate reduction by Desulfovibrio ferrophilus IS5 biofilms.

Abstract:

:Direct electron uptake is emerging as a key process for electron transfer in anaerobic microbial communities, both between species and from extracellular sources, such as zero-valent iron (Fe0 ) or cathodic surfaces. In this study, we investigated cathodic electron uptake by Fe0 -corroding Desulfovibrio ferrophilus IS5 and showed that electron uptake is dependent on direct cell contact via a biofilm on the cathode surface rather than through secreted intermediates. Induction of cathodic electron uptake by lactate-starved D. ferrophilus IS5 cells resulted in the expression of all components necessary for electron uptake; however, protein synthesis was required for full biofilm formation. Notably, proteinase K treatment uncoupled electron uptake from biofilm formation, likely through proteolytic degradation of proteinaceous components of the electron uptake machinery. We also showed that cathodic electron uptake is dependent on SO42- reduction. The insensitivity of Fe0 corrosion to proteinase K treatment suggests that electron uptake from a cathode might involve different mechanism(s) than those involved in Fe0 corrosion.

journal_name

Environ Microbiol

authors

McCully AL,Spormann AM

doi

10.1111/1462-2920.15235

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

4794-4807

issue

11

eissn

1462-2912

issn

1462-2920

journal_volume

22

pub_type

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