MerF is a novel regulator of deep-sea Pseudomonas stutzeri flagellum biogenesis and motility.

Abstract:

:MerF, a proposed bacterial mercury transporter, was surprisingly found to play key roles in the flagellum biogenesis and motility but not mercuric resistance of the deep-sea bacterium Pseudomonas stutzeri 273 in our previous study. However, the mechanism behind this interesting discovery has not been elucidated. Here, we firstly applied the combined transcriptomic and proteomic analysis to the P. stutzeri 273 wild type and merF deletion mutant. The results showed that expressions of extracellular flagellar components and FliS, a key factor controlling the biogenesis of extracellular flagellar filament, were significantly downregulated in the merF deletion mutant. In combination of genetic and biochemical methods, MerF was further demonstrated to regulate the expression of fliS via directly binding to its promoter, which is consistent with the discovery that MerF is essential for bacterial flagellum biogenesis and motility. Importantly, the expression of merF and fliS could be simultaneously upregulated by different heavy metals and MerF homologues exist in both bacterial and archaeal domains. To the best of our knowledge, this is the first report linking the heavy metal transporter and the flagellum biogenesis and motility in microorganisms, which provides a good model to investigate the unexplored adaptation strategies of deep-sea microbes against harsh conditions.

journal_name

Environ Microbiol

authors

Zheng R,Wu S,Sun C

doi

10.1111/1462-2920.15275

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

110-125

issue

1

eissn

1462-2912

issn

1462-2920

journal_volume

23

pub_type

杂志文章
  • Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans.

    abstract::In diseased piglets from two Dutch pig-breeding farms with neonatal diarrhoea for more than a year, culture and PCR analyses identified the involved microorganism as Clostridium difficile PCR ribotype 078 harbouring toxin A (tcdA) and B (tcdB), and binary toxin genes. Isolated strains showed a 39 bp deletion in the tc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01790.x

    authors: Debast SB,van Leengoed LA,Goorhuis A,Harmanus C,Kuijper EJ,Bergwerff AA

    更新日期:2009-02-01 00:00:00

  • Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields.

    abstract::The stimulation of bacteria capable of reducing soluble U(VI) to sparingly soluble U(IV) is a promising approach for containing U(VI) plumes. Anaeromyxobacter dehalogenans is capable of mediating this activity; however, its ability to couple U(VI) reduction to growth has not been established. Monitoring the increase i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01405.x

    authors: Sanford RA,Wu Q,Sung Y,Thomas SH,Amos BK,Prince EK,Löffler FE

    更新日期:2007-11-01 00:00:00

  • Reduction of (per)chlorate by a novel organism isolated from paper mill waste.

    abstract::As part of a study on the microbiology of chlorate reduction, several new dissimilatory chlorate-reducing bacteria were isolated from a broad diversity of environments. One of these, strain CKB, was selected for a more complete characterization. Strain CKB was enriched and isolated from paper mill waste with acetate a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.1999.00042.x

    authors: Bruce RA,Achenbach LA,Coates JD

    更新日期:1999-08-01 00:00:00

  • Seasonal assemblages and short-lived blooms in coastal north-west Atlantic Ocean bacterioplankton.

    abstract::Temperate oceans are inhabited by diverse and temporally dynamic bacterioplankton communities. However, the role of the environment, resources and phytoplankton dynamics in shaping marine bacterioplankton communities at different time scales remains poorly constrained. Here, we combined time series observations (time ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12629

    authors: El-Swais H,Dunn KA,Bielawski JP,Li WK,Walsh DA

    更新日期:2015-10-01 00:00:00

  • Methanogenic toluene metabolism: community structure and intermediates.

    abstract::Toluene is a model compound used to study the anaerobic biotransformation of aromatic hydrocarbons. Reports indicate that toluene is transformed via fumarate addition to form benzylsuccinate or by unknown mechanisms to form hydroxylated intermediates under methanogenic conditions. We investigated the mechanism(s) of s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02631.x

    authors: Fowler SJ,Dong X,Sensen CW,Suflita JM,Gieg LM

    更新日期:2012-03-01 00:00:00

  • Mixing of water masses caused by a drifting iceberg affects bacterial activity, community composition and substrate utilization capability in the Southern Ocean.

    abstract::The number of icebergs produced from ice-shelf disintegration has increased over the past decade in Antarctica. These drifting icebergs mix the water column, influence stratification and nutrient condition, and can affect local productivity and food web composition. Data on whether icebergs affect bacterioplankton fun...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13769

    authors: Dinasquet J,Richert I,Logares R,Yager P,Bertilsson S,Riemann L

    更新日期:2017-06-01 00:00:00

  • Systematic characterization of the bZIP transcription factor gene family in the rice blast fungus, Magnaporthe oryzae.

    abstract::Regulatory roles of the basic leucine zipper (bZIP) transcription factors (TFs) in fungi have been identified in diverse cellular processes such as development, nutrient utilization and various stress responses. In this study, the 22 Magnaporthe oryzae genes encoding bZIP TFs were systematically characterized. Phyloge...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12633

    authors: Kong S,Park SY,Lee YH

    更新日期:2015-04-01 00:00:00

  • Functional surface proteomic profiling reveals the host heat-shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages.

    abstract::Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species of the order Mucorales. Immune cells of the innate immune system serve as the first line of defence against inhaled spores. Alveolar macrophages were challenged with the mucoralean fungus Lichtheimia corymbifera an...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15140

    authors: Hassan MIA,Kruse JM,Krüger T,Dahse HM,Cseresnyés Z,Blango MG,Slevogt H,Hörhold F,Ast V,König R,Figge MT,Kniemeyer O,Brakhage AA,Voigt K

    更新日期:2020-09-01 00:00:00

  • Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages.

    abstract::Sulphoquinovosyldiacylglycerols (SQDG) are polar sulphur-containing membrane lipids, whose presence has been related to a microbial strategy to adapt to phosphate deprivation. In this study, we have targeted the sqdB gene coding the uridine 5'-diphosphate-sulphoquinovose (UDP-SQ) synthase involved in the SQDG biosynth...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12202

    authors: Villanueva L,Bale N,Hopmans EC,Schouten S,Damsté JS

    更新日期:2014-03-01 00:00:00

  • A periplasmic arsenite-binding protein involved in regulating arsenite oxidation.

    abstract::Arsenic (As) is the most common toxic element in the environment, ranking first on the Superfund List of Hazardous Substances. Microbial redox transformations are the principal drivers of As chemical speciation, which in turn dictates As mobility and toxicity. Consequently, in order to manage or remediate environmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02672.x

    authors: Liu G,Liu M,Kim EH,Maaty WS,Bothner B,Lei B,Rensing C,Wang G,McDermott TR

    更新日期:2012-07-01 00:00:00

  • The hidden lifestyles of Bacillus cereus and relatives.

    abstract::Bacillus cereus sensu lato, the species group comprising Bacillus anthracis, Bacillus thuringiensis and B. cereus (sensu stricto), has previously been scrutinized regarding interspecies genetic correlation and pathogenic characteristics. So far, little attention has been paid to analysing the biological and ecological...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1462-2920.2003.00461.x

    authors: Jensen GB,Hansen BM,Eilenberg J,Mahillon J

    更新日期:2003-08-01 00:00:00

  • Impact of different bioenergy crops on N-cycling bacterial and archaeal communities in soil.

    abstract::Biomass production for bioenergy may change soil microbes and influence ecosystem properties. To explore the impact of different bioenergy cropping systems on soil microorganisms, the compositions and quantities of soil microbial communities (16S rRNA gene) and N-cycling functional groups (nifH, bacterial amoA, archae...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02844.x

    authors: Mao Y,Yannarell AC,Davis SC,Mackie RI

    更新日期:2013-03-01 00:00:00

  • Evolutionary innovation: a bone-eating marine symbiosis.

    abstract::Symbiotic associations between microbes and invertebrates have resulted in some of the most unusual physiological and morphological adaptations that have evolved in the animal world. We document a new symbiosis between marine polychaetes of the genus Osedax and members of the bacterial group Oceanospirillales, known f...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00824.x

    authors: Goffredi SK,Orphan VJ,Rouse GW,Jahnke L,Embaye T,Turk K,Lee R,Vrijenhoek RC

    更新日期:2005-09-01 00:00:00

  • Transcription of a 'photosynthetic' T4-type phage during infection of a marine cyanobacterium.

    abstract::The transcription of S-PM2 phage following infection of Synechococcus sp. WH7803, a marine cyanobacterium, was analysed by quantitative real-time PCR. Unlike the distantly related coliphage T4, there were only two (early and late) instead of three (early, middle and late) classes of transcripts during the developmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00969.x

    authors: Clokie MR,Shan J,Bailey S,Jia Y,Krisch HM,West S,Mann NH

    更新日期:2006-05-01 00:00:00

  • A novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated 'Brachyspira suanatina' sp. nov.

    abstract::Atypical, strongly haemolytic porcine isolates of intestinal spirochaetes differing genetically from Brachyspira hyodysenteriae were identified and characterized. The isolates were subjected to culture and biochemical tests, antimicrobial susceptibility testing and molecular analyses. None of four species-specific pol...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01220.x

    authors: Råsbäck T,Jansson DS,Johansson KE,Fellström C

    更新日期:2007-04-01 00:00:00

  • Oligotrophic lagoons of the South Pacific Ocean are home to a surprising number of novel eukaryotic microorganisms.

    abstract::The diversity of microbial eukaryotes was surveyed by environmental sequencing from tropical lagoon sites of the South Pacific, collected through the American Museum of Natural History (AMNH)'s Explore21 expedition to the Solomon Islands in September 2013. The sampled lagoons presented low nutrient concentrations typi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13523

    authors: Kim E,Sprung B,Duhamel S,Filardi C,Kyoon Shin M

    更新日期:2016-12-01 00:00:00

  • Does quorum sensing interference affect the fitness of bacterial pathogens in the real world?

    abstract::Many bacterial pathogens rely on quorum sensing to control virulence gene expression. Based on numerous experiments conducted under well-defined conditions, quorum sensing interference is considered as a promising strategy to tackle infections and thus might have the potential to (partially) replace antibiotics. Despi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14446

    authors: Liu Y,Qin Q,Defoirdt T

    更新日期:2018-11-01 00:00:00

  • Polymorphism and recombination for rDNA in the putatively asexual microsporidian Nosema ceranae, a pathogen of honeybees.

    abstract::Nosema ceranae is currently one of the major pathogens of honeybees, related to the worldwide colony losses phenomenon. The genotyping of strains based on ribosomal DNA (rDNA) can be misleading if the repeated units are not identical. The analysis of cloned rDNA fragments containing the intergenic spacer (IGS) and par...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02311.x

    authors: Sagastume S,Del Águila C,Martín-Hernández R,Higes M,Henriques-Gil N

    更新日期:2011-01-01 00:00:00

  • Physiological and cell morphology adaptation of Bacillus subtilis at near-zero specific growth rates: a transcriptome analysis.

    abstract::Nutrient scarcity is a common condition in nature, but the resulting extremely low growth rates (below 0.025 h(-1) ) are an unexplored research area in Bacillus subtilis. To understand microbial life in natural environments, studying the adaptation of B. subtilis to near-zero growth conditions is relevant. To this end...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12676

    authors: Overkamp W,Ercan O,Herber M,van Maris AJ,Kleerebezem M,Kuipers OP

    更新日期:2015-02-01 00:00:00

  • Chlorobaculum tepidum growth on biogenic S(0) as the sole photosynthetic electron donor.

    abstract::The green sulfur bacteria, the Chlorobi, are phototrophic bacteria that oxidize sulfide and deposit extracellular elemental sulfur globules [S(0)]. These are subsequently consumed after sulfide is exhausted. S(0) globules from a Chlorobaculum tepidum mutant strain were purified and used to show that the wild-type stra...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12995

    authors: Hanson TE,Bonsu E,Tuerk A,Marnocha CL,Powell DH,Chan CS

    更新日期:2016-09-01 00:00:00

  • Fungal volatiles mediate cheese rind microbiome assembly.

    abstract::In vitro studies in plant, soil, and human systems have shown that microbial volatiles can mediate microbe-microbe or microbe-host interactions. These previous studies have often used artificially high concentrations of volatiles compared to in situ systems and have not demonstrated the roles volatiles play in mediati...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15223

    authors: Cosetta CM,Kfoury N,Robbat A,Wolfe BE

    更新日期:2020-11-01 00:00:00

  • Large-scale distribution and activity patterns of an extremely low-light-adapted population of green sulfur bacteria in the Black Sea.

    abstract::The Black Sea chemocline represents the largest extant habitat of anoxygenic phototrophic bacteria and harbours a monospecific population of Chlorobium phylotype BS-1. High-sensitivity measurements of underwater irradiance and sulfide revealed that the optical properties of the overlying water column were similar acro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02178.x

    authors: Marschall E,Jogler M,Hessge U,Overmann J

    更新日期:2010-05-01 00:00:00

  • Distribution of aerobic anoxygenic phototrophs in temperate freshwater systems.

    abstract::The presence of aerobic anoxygenic phototrophs (AAPs) was recently reported from various marine environments; however, there is little information regarding their distribution in fresh waters. We surveyed a number of freshwater systems in central Europe, by infra-red fluorometry, infra-red epifluorescence microscopy, ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01615.x

    authors: Masín M,Nedoma J,Pechar L,Koblízek M

    更新日期:2008-08-01 00:00:00

  • An AbrB-like protein might be involved in the regulation of cylindrospermopsin production by Aphanizomenon ovalisporum.

    abstract::Certain filamentous cyanobacteria, including Aphanizomenon ovalisporum, are potentially toxic owing to the formation of the hepatotoxin cylindrospermopsin. We previously identified a gene cluster in A. ovalisporum likely to be involved in cylindrospermopsin biosynthesis, including amidinotransferase (aoaA) and polyket...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01519.x

    authors: Shalev-Malul G,Lieman-Hurwitz J,Viner-Mozzini Y,Sukenik A,Gaathon A,Lebendiker M,Kaplan A

    更新日期:2008-04-01 00:00:00

  • Isolation and molecular detection of methylotrophic bacteria occurring in the human mouth.

    abstract::Diverse methylotrophic bacteria were isolated from the tongue, and supra- and subgingival plaque in the mouths of volunteers and patients with periodontitis. One-carbon compounds such as dimethylsulfide in the mouth are likely to be used as growth substrates for these organisms. Methylotrophic strains of Bacillus, Bre...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00805.x

    authors: Anesti V,McDonald IR,Ramaswamy M,Wade WG,Kelly DP,Wood AP

    更新日期:2005-08-01 00:00:00

  • Quantitative biogeography of picoprasinophytes establishes ecotype distributions and significant contributions to marine phytoplankton.

    abstract::Bathycoccus and Ostreococcus are broadly distributed marine picoprasinophyte algae. We enumerated small phytoplankton using flow cytometry and qPCR assays for phylogenetically distinct Bathycoccus clades BI and BII and Ostreococcus clades OI and OII. Among 259 photic-zone samples from transects and time-series, Ostreo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13812

    authors: Limardo AJ,Sudek S,Choi CJ,Poirier C,Rii YM,Blum M,Roth R,Goodenough U,Church MJ,Worden AZ

    更新日期:2017-08-01 00:00:00

  • Pirellula and OM43 are among the dominant lineages identified in an Oregon coast diatom bloom.

    abstract::Although bacterioplankton and phytoplankton are generally perceived as closely linked in marine systems, specific interactions between discrete bacterioplankton and phytoplankton populations are largely unknown. However, measurements of bacterioplankton distributions during phytoplankton blooms may indicate specific m...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01029.x

    authors: Morris RM,Longnecker K,Giovannoni SJ

    更新日期:2006-08-01 00:00:00

  • Asaia, a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders.

    abstract::Bacterial symbionts of insects have been proposed for blocking transmission of vector-borne pathogens. However, in many vector models the ecology of symbionts and their capability of cross-colonizing different hosts, an important feature in the symbiotic control approach, is poorly known. Here we show that the acetic ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02048.x

    authors: Crotti E,Damiani C,Pajoro M,Gonella E,Rizzi A,Ricci I,Negri I,Scuppa P,Rossi P,Ballarini P,Raddadi N,Marzorati M,Sacchi L,Clementi E,Genchi M,Mandrioli M,Bandi C,Favia G,Alma A,Daffonchio D

    更新日期:2009-12-01 00:00:00

  • High temporal variability in commensal Escherichia coli strain communities of a herbivorous marsupial.

    abstract::Although Escherichia coli is an important model organism for bacterial research, few studies have explored the nature of temporal variation in E. coli strains within the intestinal tracts of host individuals. In this study the E. coli strains of 54 mountain brushtail possums were sampled on four occasions during a yea...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12088

    authors: Blyton MD,Banks SC,Peakall R,Gordon DM

    更新日期:2013-08-01 00:00:00

  • Antagonistic interactions among coral-associated bacteria.

    abstract::Reef-building corals are comprised of close associations between the coral animal, symbiotic zooxanthellae, and a diversity of associated microbes (including Bacteria, Archaea and Fungi). Together, these comprise the coral holobiont - a paradigm that emphasizes the potential contributions of each component to the over...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02027.x

    authors: Rypien KL,Ward JR,Azam F

    更新日期:2010-01-01 00:00:00