The Crc and Hfq proteins of Pseudomonas putida cooperate in catabolite repression and formation of ribonucleic acid complexes with specific target motifs.

Abstract:

:The Crc protein is a global regulator that has a key role in catabolite repression and optimization of metabolism in Pseudomonads. Crc inhibits gene expression post-transcriptionally, preventing translation of mRNAs bearing an AAnAAnAA motif [the catabolite activity (CA) motif] close to the translation start site. Although Crc was initially believed to bind RNA by itself, this idea was recently challenged by results suggesting that a protein co-purifying with Crc, presumably the Hfq protein, could account for the detected RNA-binding activity. Hfq is an abundant protein that has a central role in post-transcriptional gene regulation. Herein, we show that the Pseudomonas putida Hfq protein can recognize the CA motifs of RNAs through its distal face and that Crc facilitates formation of a more stable complex at these targets. Crc was unable to bind RNA in the absence of Hfq. However, pull-down assays showed that Crc and Hfq can form a co-complex with RNA containing a CA motif in vitro. Inactivation of the hfq or the crc gene impaired catabolite repression to a similar extent. We propose that Crc and Hfq cooperate in catabolite repression, probably through forming a stable co-complex with RNAs containing CA motifs to result in inhibition of translation initiation.

journal_name

Environ Microbiol

authors

Moreno R,Hernández-Arranz S,La Rosa R,Yuste L,Madhushani A,Shingler V,Rojo F

doi

10.1111/1462-2920.12499

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

105-18

issue

1

eissn

1462-2912

issn

1462-2920

journal_volume

17

pub_type

杂志文章
  • Geomicrobiological exploration and characterization of a novel deep-sea hydrothermal system at the TOTO caldera in the Mariana Volcanic Arc.

    abstract::Novel hydrothermal activities accompanying effluent white smokers and elemental sulfur chimney structures at the north-east lava dome of the TOTO caldera depression in the Mariana Volcanic Arc have been explored and characterized by geochemical and microbiological surveys. White smoker hydrothermal fluids were observe...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00884.x

    authors: Nakagawa T,Takai K,Suzuki Y,Hirayama H,Konno U,Tsunogai U,Horikoshi K

    更新日期:2006-01-01 00:00:00

  • American crows as carriers of vancomycin-resistant enterococci with vanA gene.

    abstract::We studied the vanA-carrying vancomycin-resistant enterococci (VRE) isolated from American crows in the United States during the winter 2011/2012. Faecal samples from crows were cultured selectively for VRE and characterized. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to ex...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12213

    authors: Oravcova V,Zurek L,Townsend A,Clark AB,Ellis JC,Cizek A,Literak I

    更新日期:2014-04-01 00:00:00

  • Isotope analysis as a natural reaction probe to determine mechanisms of biodegradation of 1,2-dichloroethane.

    abstract::1,2-Dichloroethane (1,2-DCA), a chlorinated aliphatic hydrocarbon, is a well-known groundwater contaminant. In this study, fractionation of stable carbon isotope values of 1,2-DCA during biodegradation was used as a novel reaction probe to provide information about the mechanism of 1,2-DCA biodegradation under both ae...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01282.x

    authors: Hirschorn SK,Dinglasan-Panlilio MJ,Edwards EA,Lacrampe-Couloume G,Sherwood Lollar B

    更新日期:2007-07-01 00:00:00

  • Physiological and molecular characterization of a microbial community established in unsaturated, petroleum-contaminated soil.

    abstract::The microbial communities established in soil samples from an unsaturated, petroleum-contaminated zone and from an adjacent uncontaminated site were characterized by physiological and molecular approaches. Possible electron acceptors such as sulfate and nitrate had been completely depleted in these soil samples. Slurr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00754.x

    authors: Kasai Y,Takahata Y,Hoaki T,Watanabe K

    更新日期:2005-06-01 00:00:00

  • Methylation is the initial reaction in anaerobic naphthalene degradation by a sulfate-reducing enrichment culture.

    abstract::The sulfate-reducing culture N47 can utilize naphthalene or 2-methylnaphthalene as the sole carbon source and electron donor. Here we show that the initial reaction in the naphthalene degradation pathway is a methylation to 2-methylnaphthalene which then undergoes the subsequent oxidation to the central metabolite 2-n...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00900.x

    authors: Safinowski M,Meckenstock RU

    更新日期:2006-02-01 00:00:00

  • Expression and regulation of the mer operon in Thermus thermophilus.

    abstract::Mercury (Hg) is a highly toxic and widely distributed heavy metal, which some Bacteria and Archaea detoxify by the reduction of ionic Hg (Hg[II]) to the elemental volatile form, Hg(0). This activity is specified by the mer operon. The mer operon of the deeply branching thermophile Thermus thermophilus HB27 encodes for...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14953

    authors: Norambuena J,Miller M,Boyd JM,Barkay T

    更新日期:2020-04-01 00:00:00

  • Microbiota associated with Mollitrichosiphum aphids (Hemiptera: Aphididae: Greenideinae): diversity, host species specificity and phylosymbiosis.

    abstract::Symbiotic association is universal in nature, and an array of symbionts play a crucial part in host life history. Aphids and their diverse symbionts have become a good model system to study insect-symbiont interactions. Previous symbiotic diversity surveys have mainly focused on a few aphid clades, and the relative im...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15391

    authors: Qin M,Chen J,Xu S,Jiang L,Qiao G

    更新日期:2021-01-07 00:00:00

  • Free Shiga toxin bacteriophages isolated from sewage showed diversity although the stx genes appeared conserved.

    abstract::Phages carrying the stx2 gene were detected in a range of sewage samples using a plaque hybridization-based method. After detection, phages were isolated and propagated with a laboratory strain of Escherichia coli as host for characterization purposes. Although it was not possible to conduct propagation or transductio...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00604.x

    authors: Muniesa M,Serra-Moreno R,Jofre J

    更新日期:2004-07-01 00:00:00

  • Recognition cascade and metabolite transfer in a marine bacteria-phytoplankton model system.

    abstract::The trophic linkage between marine bacteria and phytoplankton in the surface ocean is a key step in the global carbon cycle, with almost half of marine primary production transformed by heterotrophic bacterioplankton within hours to weeks of fixation. Early studies conceptualized this link as the passive addition and ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13834

    authors: Durham BP,Dearth SP,Sharma S,Amin SA,Smith CB,Campagna SR,Armbrust EV,Moran MA

    更新日期:2017-09-01 00:00:00

  • Novel acid resistance genes from the metagenome of the Tinto River, an extremely acidic environment.

    abstract::Microorganisms that thrive in acidic environments are endowed with specialized molecular mechanisms to survive under this extremely harsh condition. In this work, we performed functional screening of six metagenomic libraries from planktonic and rhizosphere microbial communities of the Tinto River, an extremely acidic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12021

    authors: Guazzaroni ME,Morgante V,Mirete S,González-Pastor JE

    更新日期:2013-04-01 00:00:00

  • A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality.

    abstract::Biological membranes have evolved different mechanisms to modify their composition in response to chemical stimuli in a process called 'homeoviscous adaptation'. Among these mechanisms, modifications in the ratio of saturated/unsaturated fatty acids and in cis/trans fatty acid isomers, cyclopropanation and changes in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01236.x

    authors: Bernal P,Muñoz-Rojas J,Hurtado A,Ramos JL,Segura A

    更新日期:2007-05-01 00:00:00

  • Elucidation of sevadicin, a novel non-ribosomal peptide secondary metabolite produced by the honey bee pathogenic bacterium Paenibacillus larvae.

    abstract::American foulbrood (AFB) caused by the bee pathogenic bacterium Paenibacillus larvae is the most devastating bacterial disease of honey bees worldwide. From AFB-dead larvae, pure cultures of P. larvae can normally be cultivated indicating that P. larvae is able to defend its niche against all other bacteria present. R...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12417

    authors: Garcia-Gonzalez E,Müller S,Ensle P,Süssmuth RD,Genersch E

    更新日期:2014-05-01 00:00:00

  • Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- and bathypelagic waters of the (sub)tropical Atlantic.

    abstract::The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of D- and L-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35 degrees N to 5 degrees S). Fluorescence in situ hybridization (FISH) rev...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01627.x

    authors: Varela MM,van Aken HM,Herndl GJ

    更新日期:2008-07-01 00:00:00

  • Following spread of fire blight in Western, Central and Southern Europe by molecular differentiation of Erwinia amylovora strains with PFGE analysis.

    abstract::Fire blight has been detected recently in several areas of northern Spain and north-eastern Italy. To follow spread of the disease within Europe, more than 120 Erwinia amylovora strains isolated from 1957-1900 in England, France, Germany, The Netherlands, Belgium, Poland, Italy and Spain were assayed using pulsed-fiel...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00277.x

    authors: Jock S,Donat V,López MM,Bazzi C,Geider K

    更新日期:2002-02-01 00:00:00

  • The molecular ecology of Microcystis sp. blooms in the San Francisco Estuary.

    abstract::Harmful blooms of the cyanobacterium Microcystis sp. have become increasingly pervasive in the San Francisco Estuary Delta (USA) since the early 2000s and their rise has coincided with substantial decreases in several important fish species. Direct and indirect effects Microcystis blooms may have on the Delta food web...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13860

    authors: Otten TG,Paerl HW,Dreher TW,Kimmerer WJ,Parker AE

    更新日期:2017-09-01 00:00:00

  • Lentisphaera araneosa gen. nov., sp. nov, a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae.

    abstract::Two phylogenetically distinct marine strains producing transparent exopolymers (TEP), designated HTCC2155(T) and HTCC2160, were cultivated from Oregon coast seawater by dilution to extinction in a high throughput culturing format. When cultured in low-nutrient seawater media, these strains copiously produced Alcian Bl...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00614.x

    authors: Cho JC,Vergin KL,Morris RM,Giovannoni SJ

    更新日期:2004-06-01 00:00:00

  • Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment.

    abstract::Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14297

    authors: Müller AL,Pelikan C,de Rezende JR,Wasmund K,Putz M,Glombitza C,Kjeldsen KU,Jørgensen BB,Loy A

    更新日期:2018-08-01 00:00:00

  • Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities.

    abstract::While the dynamics of microbial community assembly driven by environmental perturbations have been extensively studied, our understanding is far from complete, particularly for light-induced perturbations. Extremely halophilic communities thriving in coastal solar salterns are mainly influenced by two environmental fa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14790

    authors: Viver T,Orellana LH,Díaz S,Urdiain M,Ramos-Barbero MD,González-Pastor JE,Oren A,Hatt JK,Amann R,Antón J,Konstantinidis KT,Rosselló-Móra R

    更新日期:2019-11-01 00:00:00

  • 'Candidatus Lumbricincola', a novel lineage of uncultured Mollicutes from earthworms of family Lumbricidae.

    abstract::Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01837.x

    authors: Nechitaylo TY,Timmis KN,Golyshin PN

    更新日期:2009-04-01 00:00:00

  • Oligotrophic lagoons of the South Pacific Ocean are home to a surprising number of novel eukaryotic microorganisms.

    abstract::The diversity of microbial eukaryotes was surveyed by environmental sequencing from tropical lagoon sites of the South Pacific, collected through the American Museum of Natural History (AMNH)'s Explore21 expedition to the Solomon Islands in September 2013. The sampled lagoons presented low nutrient concentrations typi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13523

    authors: Kim E,Sprung B,Duhamel S,Filardi C,Kyoon Shin M

    更新日期:2016-12-01 00:00:00

  • Characterization of a homogeneous taxonomic group of marine magnetotactic cocci within a low tide zone in the China Sea.

    abstract::Magnetotactic bacteria are a heterologous group of motile prokaryotes, ubiquitous in aquatic habitats and cosmopolitan in distribution. Here, we studied the diversity of magnetotactic bacteria in a seawater pond within an intertidal zone at Huiquan Bay in the China Sea. The pond is composed of a permanently submerged ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01532.x

    authors: Pan H,Zhu K,Song T,Yu-Zhang K,Lefèvre C,Xing S,Liu M,Zhao S,Xiao T,Wu LF

    更新日期:2008-05-01 00:00:00

  • What distinguishes cyanobacteria able to revive after desiccation from those that cannot: the genome aspect.

    abstract::Filamentous cyanobacteria are the main founders and primary producers in biological desert soil crusts (BSCs) and are likely equipped to cope with one of the harshest environmental conditions on earth including daily hydration/dehydration cycles, high irradiance and extreme temperatures. Here, we resolved and report o...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13486

    authors: Murik O,Oren N,Shotland Y,Raanan H,Treves H,Kedem I,Keren N,Hagemann M,Pade N,Kaplan A

    更新日期:2017-02-01 00:00:00

  • Microbial diversity in ikaite tufa columns: an alkaline, cold ecological niche in Greenland.

    abstract::Ikaite tufa columns from the Ikka Fjord in south-western Greenland constitute a natural, stable environment at low temperature and with a pH ranging from neutral at the exterior to very alkaline (pH 10.4) at the interior of the column. Phylogenetic analysis of culturable organisms revealed ten different isolates repre...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00327.x

    authors: Stougaard P,Jørgensen F,Johnsen MG,Hansen OC

    更新日期:2002-08-01 00:00:00

  • Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration.

    abstract::Nostoc flagelliforme is well known for its strong ecological adaptability in inhabiting desert biological soil crusts. However, the mechanism of its recovery from quiescent to active state after prolonged dormancy remains poorly characterized. Especially how exoproteome be related to the adaptive strategies and partic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14931

    authors: Wang B,Yang J,Xu C,Yi L,Wan C

    更新日期:2020-04-01 00:00:00

  • Opportunistic bacteria use quorum sensing to disturb coral symbiotic communities and mediate the occurrence of coral bleaching.

    abstract::Coral associated microorganisms, especially some opportunistic pathogens can utilize quorum-sensing (QS) signals to affect population structure and host health. However, direct evidence about the link between coral bleaching and dysbiotic microbiomes under QS regulation was lacking. Here, using 11 opportunistic bacter...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15009

    authors: Zhou J,Lin ZJ,Cai ZH,Zeng YH,Zhu JM,Du XP

    更新日期:2020-05-01 00:00:00

  • Transcriptomic insights into the alternative splicing-mediated adaptation of the entomopathogenic fungus Beauveria bassiana to host niches: autophagy-related gene 8 as an example.

    abstract::Alternative splicing (AS) regulates various biological processes in fungi by extending the cellular proteome. However, comprehensive studies investigating AS in entomopathogenic fungi are lacking. Based on transcriptome data obtained via dual RNA-seq, the first overview of AS events was developed for Beauveria bassian...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13862

    authors: Dong WX,Ding JL,Gao Y,Peng YJ,Feng MG,Ying SH

    更新日期:2017-10-01 00:00:00

  • Reduction of (per)chlorate by a novel organism isolated from paper mill waste.

    abstract::As part of a study on the microbiology of chlorate reduction, several new dissimilatory chlorate-reducing bacteria were isolated from a broad diversity of environments. One of these, strain CKB, was selected for a more complete characterization. Strain CKB was enriched and isolated from paper mill waste with acetate a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.1999.00042.x

    authors: Bruce RA,Achenbach LA,Coates JD

    更新日期:1999-08-01 00:00:00

  • Shifts in methanogen community structure and function associated with long-term manipulation of sulfate and salinity in a hypersaline microbial mat.

    abstract::Methanogenesis was characterized in hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico both in situ and after long-term manipulation in a greenhouse environment. Substrate addition experiments indicate methanogenesis to occur primarily through the catabolic demethylation of non-competitive sub...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01459.x

    authors: Smith JM,Green SJ,Kelley CA,Prufert-Bebout L,Bebout BM

    更新日期:2008-02-01 00:00:00

  • Ammonia-oxidizing Crenarchaeota and nitrification inside the tissue of a colonial ascidian.

    abstract::Marine Crenarchaeota represent an abundant component of the oceanic microbiota that play an important role in the global nitrogen cycle. Here we report the association of the colonial ascidian Cystodytes dellechiajei with putative ammonia-oxidizing Crenarchaeota that could actively be involved in nitrification inside ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01761.x

    authors: Martínez-García M,Stief P,Díaz-Valdés M,Wanner G,Ramos-Esplá A,Dubilier N,Antón J

    更新日期:2008-11-01 00:00:00

  • Fungal ecological strategies reflected in gene transcription - a case study of two litter decomposers.

    abstract::Microbial communities interplay with their environment through their functional traits that can be a response or an effect on the environment. Here, we explore how a functional trait-the decomposition of organic matter, can be addressed based on genetic markers and how the expression of these markers reflect ecologica...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14873

    authors: Barbi F,Kohler A,Barry K,Baskaran P,Daum C,Fauchery L,Ihrmark K,Kuo A,LaButti K,Lipzen A,Morin E,Grigoriev IV,Henrissat B,Lindahl BD,Martin F

    更新日期:2020-03-01 00:00:00