Abstract:
:One of the hallmarks of Pseudomonas aeruginosa infection in cystic fibrosis (CF) patients is very-high-cell-density (HCD) replication in the lung, allowing this bacterium to induce virulence controlled by the quorum-sensing systems. However, the nutrient sources sustaining HCD replication in this chronic infection are largely unknown. Here, we performed microarray studies of P. aeruginosa directly isolated from the lungs of CF patients to demonstrate its metabolic capability and virulence in vivo. In vivo microarray data, confirmed by real-time reverse transcription-PCR, indicated that the P. aeruginosa population expressed several genes for virulence, drug resistance, and utilization of multiple nutrient sources (lung surfactant lipids and amino acids) contributing to HCD replication. The most abundant lung surfactant lipid molecule, phosphatidylcholine (PC), induces key genes of P. aeruginosa pertinent to PC degradation in vitro as well as in vivo within the lungs of CF patients. The results support recent research indicating that P. aeruginosa exists in the lungs of CF patients as a diverse population with full virulence potential. The data also indicate that there is deregulation of several pathways, suggesting that there is in vivo evolution by deregulation of a large portion of the transcriptome during chronic infection in CF patients. To our knowledge, this is the first in vivo transcriptome analysis of P. aeruginosa in a natural infection in CF patients, and the results indicate several important aspects of P. aeruginosa pathogenesis, drug resistance, nutrient utilization, and general metabolism within the lungs of CF patients.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Son MS,Matthews WJ Jr,Kang Y,Nguyen DT,Hoang TTdoi
10.1128/IAI.01807-06subject
Has Abstractpub_date
2007-11-01 00:00:00pages
5313-24issue
11eissn
0019-9567issn
1098-5522pii
IAI.01807-06journal_volume
75pub_type
杂志文章abstract::By screening a genomic lambda library of Streptococcus equi subsp. zooepidemicus, we have cloned and sequenced a gene, termed fnz, encoding a fibronectin (Fn)-binding protein called FNZ. On the basis of the deduced amino acid sequence of FNZ, the mature protein has a molecular mass of approximately 61 kDa. Analysis of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.3993-3999.1996
更新日期:1996-10-01 00:00:00
abstract::Toxic shock syndrome-associated staphylococcal and streptococcal exotoxins were tested for an ability to induce the production of tumor necrosis factor (TNF). Staphylococcal enterotoxins B and C1, along with streptococcal pyrogenic exotoxin A, all induced TNF production in a dose-dependent manner, with production peak...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.291-294.1989
更新日期:1989-01-01 00:00:00
abstract::Bordetella bronchiseptica is closely related to Bordetella pertussis, which produces respiratory disease primarily in mammals other than humans. However, its importance as a human pathogen is being increasingly recognized. Although a large amount of research on Bordetella has been generated regarding protein virulence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.1791-1798.2002
更新日期:2002-04-01 00:00:00
abstract::Persistent infection with Borrelia burgdorferi in the presence of a vigorous host immune response has been demonstrated in humans and in animal models of Lyme disease. Long-term persistence of B. burgdorferi was documented recently in our studies of BALB/c and C3H mice infected with cloned and uncloned strains of B. b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3521-3527.1994
更新日期:1994-08-01 00:00:00
abstract::Protection against colibacillosis in neonatal piglets was obtained by immunization of pregnant dams with procholeragenoid. Procholeragenoid is a stable high-molecular-weight aggregate of cholera toxin formed during the heating of cholera toxin. Procholeragenoid retained approximately 1% of the toxicity of native toxin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.887-894.1982
更新日期:1982-03-01 00:00:00
abstract::Hydroxystilbamidine (HSB) is a potent suppressor of the plaque-forming cell response of mice injected with heterologous erythrocytes. HSB, given in varying doses and injection schedules, suppressed both the primary and secondary immune responses to bovine serum albumin. Apparently the effect is not simply a toxic effe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.441-444.1975
更新日期:1975-03-01 00:00:00
abstract::Toll-like receptor (TLR)-based signaling pathways in the host may be modulated by pathogens during the course of infection. We describe a novel immunomodulatory mechanism in which Aspergillus fumigatus conidia induce attenuation of TLR2- and TLR4-mediated interleukin (IL)-6 and IL-1beta proinflammatory responses in hu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01455-08
更新日期:2009-05-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5508-5513.1999
更新日期:1999-10-01 00:00:00
abstract::PspA is an antigenically variable surface protein of Streptococcus pneumoniae that appears to be essential for full pneumococcal virulence. In addition, monoclonal antibodies to PspA protect mice against infection with specific strains of pneumococci virulent for mice. In this study, we have isolated the 43-kDa N-term...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1285-1289.1991
更新日期:1991-04-01 00:00:00
abstract::Ureaplasma urealyticum has previously been isolated from the cultured cerebrospinal fluid of 13 of 418 newborn infants; additional bloodstream isolates were obtained from the same population. Ten of the 13 cerebrospinal fluid and 3 bloodstream isolates were available for serotyping in the present study. By the use of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3472-3474.1992
更新日期:1992-08-01 00:00:00
abstract::For the first time, hepatitis A viral antigen (HAAg) was shown in liver biopsy tissue from a patient in the acute phase of hepatitis type A by light and electron microscopy, using the peroxidase-antibody technique. Under light microscopy, the staining for HAAg appeared as a fine, granular reaction product, scattered t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.320-324.1982
更新日期:1982-04-01 00:00:00
abstract::Bacillus anthracis, the causative agent of anthrax, relies on multiple virulence factors to subvert the host immune defense. Using Caenorhabditis elegans as an infection model, we screened approximately 5,000 transposon mutants of B. anthracis Sterne for decreased virulence. One of the attenuated mutants resulted in l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01614-13
更新日期:2014-03-01 00:00:00
abstract::Iron acquisition from the host is an important step in the pathogenic process. While Yersinia pestis has multiple iron transporters, the yersiniabactin (Ybt) siderophore-dependent system plays a major role in iron acquisition in vitro and in vivo. In this study, we determined that the Ybt system is required for the us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01236-09
更新日期:2010-05-01 00:00:00
abstract::Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37 degr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7324-7331.2005
更新日期:2005-11-01 00:00:00
abstract::Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F(497), HIS-PLO.Delta P(499), and HI...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2966-2969.2003
更新日期:2003-05-01 00:00:00
abstract::Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic Escherichia coli (UPEC) being the major causative agent of the infection. Although there has been extensive research on UPEC vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-17
更新日期:2018-02-20 00:00:00
abstract::We found that when group A streptococci are cocultured with human pharyngeal cells, they upregulate and secrete a 25-kDa toxin, determined to be the bacteriophage-encoded streptococcal pyrogenic exotoxin C (SpeC). This prompted us to determine if the bacteriophage themselves are induced during coculture conditions. We...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1440-1443.2001
更新日期:2001-03-01 00:00:00
abstract::Previous studies have suggested that interleukin-4 (IL-4) has a protective effect in host defense to Borrelia burgdorferi infection, both in limiting the severity of arthritis and in controlling spirochete numbers in tissues, and a mapping study revealed suggestive linkage to a cluster of genes on mouse chromosome 11,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5603-5609.2000
更新日期:2000-10-01 00:00:00
abstract::An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and high-performance liquid chromatography on a Mono Q (anion-exchange) column. The purified cytolysin had a molecular weight of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.954-960.1988
更新日期:1988-04-01 00:00:00
abstract::The Toll-like receptor 5 (TLR5) binding site has been predicted to be in the N terminus of the flagellin molecule. In order to better define the interaction between the N-terminal amino acids of Pseudomonas aeruginosa flagellin and TLR5, site-specific mutations were generated between residues 88 and 97 of P. aeruginos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8237-8246.2005
更新日期:2005-12-01 00:00:00
abstract::An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1089-1094.1989
更新日期:1989-04-01 00:00:00
abstract::Extracellular protease activity was measured in liquid cultures of representative strains from six serotypes of Legionella pneumophila. A variety of substrates were degraded, including denatured casein, skim milk, gelatin, and hide powder azure, but not elastin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.299-302.1981
更新日期:1981-10-01 00:00:00
abstract::Staphylococcus aureus infections are an important public health concern due to their increasing incidence and high rates of mortality. The success of S. aureus as a pathogen is highly related to its enormous capacity to evade the host immune response. The critical role of tumor necrosis factor alpha (TNF-α) in the ini...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00593-13
更新日期:2013-11-01 00:00:00
abstract::Several strains of gram-negative bacteria (seven genera, eight species) isolated from patients with urinary tract infections were found to hydrolyze myeloma immunoglobulin A (IgA) protein. Human IgG and IgM and colostrum IgA were not degraded by these organisms. Examination of cleavage digests showed two fragments of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.11-13.1984
更新日期:1984-01-01 00:00:00
abstract::Two Escherichia coli strains (B/M 369 and C-35) belonging to enteropathogenic serogroup O86 were isolated from patients with infantile diarrhea and studied with respect to their cellular adherence properties. Both strains exhibited adherence (Ad+) to HEp-2 and HeLa cell monolayers in vitro and expressed mannose-resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1106-1113.1990
更新日期:1990-04-01 00:00:00
abstract::Chlamydia trachomatis infections can lead to severe chronic complications, including trachoma, ectopic pregnancy, and infertility. The only effective approach to disease control is vaccination. The goal of this work was to identify new potential vaccine candidates through a proteomics approach. We constructed a protei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00626-10
更新日期:2011-01-01 00:00:00
abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2042-2047.1990
更新日期:1990-07-01 00:00:00
abstract::Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00
abstract::The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3238-3243.1992
更新日期:1992-08-01 00:00:00
abstract::Relapsing fever (RF) is a spirochetal infection characterized by relapses of a febrile illness and spirochetemia due to the sequential appearance and disappearance of isogenic serotypes in the blood. The only difference between isogenic serotypes is the variable major outer membrane lipoprotein. In the absence of spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00538-06
更新日期:2006-11-01 00:00:00