Abstract:
:We studied the binding of dog immunoglobulins G, A, M and E to protein A and protein G. Passive cutaneous anaphylaxis (PCA) testing was used for the measurement of dog IgE and enzyme-linked immunosorbent assays (ELISA) were used for the measurements of dog IgG, IgA and IgM. Protein A from lyophilized cells of Staphylococcus aureus bound 97% of IgE, 98% of IgG, 81% of IgA, and 97% of IgM. Protein A-Sepharose CL-4B bound 87% of IgE, 100% of IgG and IgA, and 98% of IgM. In a stepwise elution with varying pH, a small amount of IgE was eluted at pH 5 and pH 6 and all the remaining Igs were eluted at pH 3 from the protein A column. In contrast to protein A, dog IgE was not bound to Protein G-Sepharose, while 100% of IgG, 95% of IgA, and 44% of IgM were bound to Protein G-Sepharose.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Peng ZK,Simons FE,Becker ABdoi
10.1016/0022-1759(91)90335-dsubject
Has Abstractpub_date
1991-12-15 00:00:00pages
255-8issue
1-2eissn
0022-1759issn
1872-7905pii
0022-1759(91)90335-Djournal_volume
145pub_type
杂志文章abstract::Whilst several centres have reported lymphocytotoxic antibody detection using single and dual fluorescent stains with analysis of the fluorescence emitted from the cell population present in a well of a multiwell plate, problems are encountered with cell concentration and light emission overlap. A method we have devel...
journal_title:Journal of immunological methods
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abstract::A fragment of antigen binding (Fab) surface display system was developed using a glycoengineered Pichia pastoris host strain genetically modified to secrete glycoproteins with mammalian mannose-type Man(5)GlcNAc(2) N-linked glycans. The surface display method described here takes advantage of a pair of coiled-coil pep...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:1992-06-24 00:00:00
abstract:INTRODUCTION:Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune ...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2017-05-01 00:00:00
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journal_title:Journal of immunological methods
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abstract::BlockLogo is a web-server application for the visualization of protein and nucleotide fragments, continuous protein sequence motifs, and discontinuous sequence motifs using calculation of block entropy from multiple sequence alignments. The user input consists of a multiple sequence alignment, selection of motif posit...
journal_title:Journal of immunological methods
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abstract::This communication describes an automated micro-adherence inhibition assay. Tumor-specific immunity was demonstrated with B16 melanoma and MCA-38 colon adenocarcinoma, both of which are syngeneic to the same strain of mouse (C57B16/J). Abrogation of the leukocyte adherence inhibition (LAI) response of sensitized leuko...
journal_title:Journal of immunological methods
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doi:10.1016/0022-1759(77)90086-2
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abstract::Six monoclonal and two polyclonal antibodies to fluorescein (FLU) were affinity purified and immobilized on Immulon 2 polystyrene as capture antibodies (CAbs): (a) by passive adsorption at pH 9.6, (b) via a streptavidin bridge to a biotinylated carrier molecule, and (c) via an antiglobulin which had been previously ad...
journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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更新日期:1992-06-24 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90100-8
更新日期:1992-07-06 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:1988-11-25 00:00:00
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journal_title:Journal of immunological methods
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2008-04-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(81)90330-6
更新日期:1981-01-01 00:00:00
abstract::Use of whole blood for lymphocyte proliferation experiments have advantages over the usual isolated lymphocyte procedure. We have systematically optimized methodology for whole blood lymphocyte proliferation in male Sprague-Dawley rats. Both phytohemagglutinin (PHA) and concanavalin A (ConA) stimulate lymphocyte blast...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(95)00084-n
更新日期:1995-08-18 00:00:00
abstract::A general affinity chromatographic method for preparation of monospecific antibody to serum haptoglobin of any species is described. Hemoglobin prepared from the species to be immunized is coupled to an organomercurical substituted agarose gel support (Affi-Gel 501). The immobilized hemoglobin binds haptoglobin with g...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90005-x
更新日期:1979-01-01 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2000-12-01 00:00:00
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journal_title:Journal of immunological methods
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abstract::The present report describes further improvements in the methodology for antigen-specific T-lymphocyte activation in vitro. Following the experimental protocol, large numbers of enriched T-lymphocytes can be obtained from the draining lymph nodes of immunized mice after purification on a nylon column. These T-cells re...
journal_title:Journal of immunological methods
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doi:10.1016/0022-1759(80)90177-5
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::A simple method to study 19S anti-SRBC antibody production is described. This technique, performed by incubation of immune spleen cells, SRBC and C in the fluid phase, utilises photomeric estimation of antibody mediated haemoglobin release. The test is quicker and more accurate than the two-step haemolytic plaque assa...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(77)90143-0
更新日期:1977-01-01 00:00:00