Multiplex serology of paraneoplastic antineuronal antibodies.

Abstract:

:Paraneoplastic neurological syndromes (PNS) are devastating neurological disorders secondary to cancer, associated with onconeural autoantibodies. Such antibodies are directed against neuronal antigens aberrantly expressed by the tumor. The detection of onconeural antibodies in a patient is extremely important in diagnosing a neurological syndrome as paraneoplastic (70% is not yet known to have cancer) and in directing the search for the underlying neoplasm. At present six onconeural antibodies are considered 'well characterized' and recognize the antigens HuD, CDR62 (Yo), amphiphysin, CRMP-5 (CV2), NOVA-1 (Ri), and Ma2. The gold standard of detection is the characteristic immunohistochemical staining pattern on brain tissue sections combined with confirmation by immunoblotting using recombinant purified proteins. Since all six onconeural antibodies are usually analyzed simultaneously and objective cut-off values for these analyses are warranted, we developed a multiplex assay based on Luminex technology. Reaction of serial dilutions of six onconeural standard sera with microsphere-bound antigens showed lower limits of detection than with Western blotting. Using the six standard sera at a dilution of 1:200, the average within-run coefficient of variation (CV) was 4% (range 1.9-7.3%). The average between-run within-day CV was 5.1% (range 2.9-6.7%) while the average between-day CV was 8.1% (range 2.8-11.6%). The shelf-life of the antigen coupled microspheres was at least two months. The sensitivity of the multiplex assay ranged from 83% (Ri) to 100% (Yo, amphiphysin, CV2) and the specificity from 96% (CV2) to 100% (Ri). In conclusion, Luminex-based multiplex serology is highly reproducible with high sensitivity and specificity for the detection of onconeural antibodies. Conventional immunoblotting for diagnosis of onconeural antibodies in the setting of a routine laboratory may be replaced by this novel, robust technology.

journal_name

J Immunol Methods

authors

Maat P,Brouwer E,Hulsenboom E,VanDuijn M,Schreurs MW,Hooijkaas H,Smitt PA

doi

10.1016/j.jim.2013.02.017

subject

Has Abstract

pub_date

2013-05-31 00:00:00

pages

125-32

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(13)00073-2

journal_volume

391

pub_type

杂志文章
  • Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells.

    abstract::Ferromagnetic iron dextran particles were prepared by reacting a mixture of ferrous chloride and ferric chloride with dextran polymers under alkaline conditions. Particles purified by gel filtration chromatography were in the size range of 30-40 nm, had an electron dense core of about 15 nm, were stable against aggreg...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90007-2

    authors: Molday RS,MacKenzie D

    更新日期:1982-08-13 00:00:00

  • The Trypanosoma lewisi immunofluorescence test: a new simple technique for simultaneous determination of total antinuclear antibodies and the detection of antibodies to double-stranded DNA.

    abstract::An indirect immunofluorescence technique was developed for the detection of antibodies to dsDNA and the simultaneous assessment of antinuclear antibodies 'in toto' (ANA). This assay was based upon the use as substrate of smears of peripheral blood derived from rats infected with Trypanosoma lewisi. T. lewisi possesses...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90192-2

    authors: Valesini G,Masala C

    更新日期:1982-01-01 00:00:00

  • Production of murine monoclonal antibodies against human thyroglobulin using an in vitro immunization procedure in serum-free medium.

    abstract::A serum-free in vitro immunization method for the generation of hybridomas producing specific antibodies to an antigen is described. The method was tested with human thyroglobulin as antigen. The serum-free medium used (Yssel et al., 1984) consisted of Iscove's modification of Dulbecco's modified Eagle's medium, suppl...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(86)90487-4

    authors: Ossendorp FA,De Boer M,Al BJ,Hilgers J,Bruning PF,Tager JM

    更新日期:1986-07-24 00:00:00

  • Evaluation of different methods for the detection of outer membrane proteins and lipopolysaccharides of Pasteurella haemolytica by immunoblotting.

    abstract::The optimal conditions for the detection of outer membrane proteins (OMPs) and lipopolysaccharide (LPS) of Pasteurella haemolytica by immunoblotting were evaluated. The variables examined included the equilibration time of the gels before transfer, composition of the transfer buffer, type of blotting membrane, blockin...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(94)90072-8

    authors: Davies RL,Parton R,Coote JG,Gibbs HA,Freer JH

    更新日期:1994-01-03 00:00:00

  • Differential binding properties of protein A and protein G for dog immunoglobulins.

    abstract::We studied the binding of dog immunoglobulins G, A, M and E to protein A and protein G. Passive cutaneous anaphylaxis (PCA) testing was used for the measurement of dog IgE and enzyme-linked immunosorbent assays (ELISA) were used for the measurements of dog IgG, IgA and IgM. Protein A from lyophilized cells of Staphylo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(91)90335-d

    authors: Peng ZK,Simons FE,Becker AB

    更新日期:1991-12-15 00:00:00

  • Vaccines for visceral leishmaniasis: A review.

    abstract::Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jim.2015.03.017

    authors: Jain K,Jain NK

    更新日期:2015-07-01 00:00:00

  • Measuring bonds between surface-associated molecules.

    abstract::Adhesive interactions play an essential role in immune function. Much information on these phenomena was recently obtained by applying sophisticated methods such as the surface forces apparatus, atomic force microscopy, lipid vesicle-based technology or flow chambers. In the present review it is shown that the use of ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审

    doi:10.1016/0022-1759(96)00103-2

    authors: Pierres A,Benoliel AM,Bongrand P

    更新日期:1996-09-27 00:00:00

  • Parameters of the labeling of mitogen-activated murine lymphocytes by [35S]methionine for two-dimensional gel electrophoresis. I. Effect of culture conditions.

    abstract::Labeling with [35S]methionine at a high specific activity is essential to the facile preparation of 2-dimensional gel electrophoretograms with the analytical 2-dimensional charge-size separation procedure (Anderson's ISODALT system). Mitogen-activated T and B lymphocytes subjected to low methionine concentrations woul...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(86)90051-7

    authors: Kettman JR,Kuhn L,Young P,Lefkovits I

    更新日期:1986-04-03 00:00:00

  • Production of glutathione-coated microtitre plates for capturing recombinant glutathione S-transferase fusion proteins as antigens in immunoassays.

    abstract::Glutathione S-transferase (GST) is commonly used as a fusion partner in producing recombinant proteins and this technology is increasingly being used to produce antigens for use in immunoassays to measure antibodies. To circumvent the requirement to purify such antigens before use, we developed a method for coupling g...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(98)00114-8

    authors: Murray AM,Kelly CD,Nussey SS,Johnstone AP

    更新日期:1998-09-01 00:00:00

  • Direct and indirect immunofluorescence analysis of bacterial populations by flow cytometry.

    abstract::Bacillus anthracis spores and Escherichia coli were stained with fluorescein-conjugated antibody using direct and indirect methods, then analyzed by means of a commercial flow cytometer. To reduce the cytometer's fluorescence component resulting from unreacted conjugate, reaction mixtures were either diluted or were c...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(87)90153-0

    authors: Phillips AP,Martin KL,Capey AJ

    更新日期:1987-08-03 00:00:00

  • A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen(s) and antibodies in tissues and fluids.

    abstract::A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein previously found to have a strong H-D a...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(85)90168-1

    authors: Mukuria JC,Naiki M,Hashimoto M,Nishiura K,Okabe M,Kato S

    更新日期:1985-06-12 00:00:00

  • Automated complement fixation test for the detection of antibodies against the core of hepatitis B virus (HBc).

    abstract::The automated complement fixation system presented in this paper differs from those described by Studievic et al. (1971) and Vargues and Henley (1974). In the one developed here only one sampler is used with a wheel presenting a double row of cups. This system can assay 60 samples per h and can be modified to double t...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(76)90183-6

    authors: Coursaget P,Maupas P,Goudeau A,Millman I

    更新日期:1976-01-01 00:00:00

  • A new method for the simultaneous assay of rosette-forming and antibody-secreting cells, utilising immune adhesion reactions in monolayer.

    abstract::Mixtures of sheep erythrocytes and immune spleen cells from mice were incubated in shallow slide chambers coated with erythrocyte ghosts or anti-mouse Ig, with poly L-lysine as a coupling agent. Antigen-binding cells and erythrocytes surrounding antibody-releasing lymphocytes became bound to the reactive surfaces by i...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(78)90180-1

    authors: Wilson AR,Cruickshank WJ,Pugh-Humphreys RG,Jeffries AH

    更新日期:1978-01-01 00:00:00

  • An enzyme-linked double antibody immunoassay to measure murine immunoglobulins--its application to determine the specific activity of radiolabeled monoclonal antibodies.

    abstract::A sensitive, simple and reproducible biotin-avidin amplified double antibody immunoassay to quantitate low concentrations of mouse immunoglobulins is described. The assay is a useful technique to measure trace levels of murine monoclonal antibodies in culture supernatants of hybridoma cells metabolically labeled with ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(85)90355-2

    authors: Ziai MR,Imberti L,Ferrone S

    更新日期:1985-10-10 00:00:00

  • A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test.

    abstract::The neutrophil respiratory burst is essential for the host's ability to kill ingested microorganisms. Several flow cytometric assays have recently been developed to measure this process. These assays are largely unvalidated. In this study a whole blood flow cytometry assay using dihydrorhodamine 123 (DHR) as a substra...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(98)00136-7

    authors: Richardson MP,Ayliffe MJ,Helbert M,Davies EG

    更新日期:1998-10-01 00:00:00

  • Development and validation of a flow cytometric method to evaluate phagocytosis of pHrodo™ BioParticles® by granulocytes in multiple species.

    abstract::Phagocytosis plays a pivotal and essential role in host immune defense, both as a focal constituent of the innate immune response and a bridging element linking innate and adaptive immunity. Phagocytosis has been demonstrated to be critical in development, tissue remodeling, wound healing and resolution of inflammatio...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2011.06.027

    authors: Neaga A,Lefor J,Lich KE,Liparoto SF,Xiao YQ

    更新日期:2013-04-30 00:00:00

  • F(ab')2 molecules made from Escherichia coli produced Fab' with hinge sequences conferring increased serum survival in an animal model.

    abstract::Fab's with hinges based on the human gamma1 sequence containing 1, 2, or 4 cysteines have been produced by high level Escherichia coli periplasmic secretion, and coupled in vitro by reduction/oxidation to form F(ab')2. We find that the F(ab')2 made with hinges containing 2 or 4 cysteines have a high level (approximate...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(98)00061-1

    authors: Humphreys DP,Vetterlein OM,Chapman AP,King DJ,Antoniw P,Suitters AJ,Reeks DG,Parton TA,King LM,Smith BJ,Lang V,Stephens PE

    更新日期:1998-08-01 00:00:00

  • An improved assay for the detection of interleukin 1.

    abstract::A 24 h, highly sensitive assay for detection of interleukin 1 (IL-1) is described. A thioguanine-resistant mutant of the murine lymphoma cell line LBRM 33 was selected (LBRM TG6). When this cell line was incubated with low concentrations of PHA and IL-1 it produced interleukin 2 (IL-2). IL-2-dependent HT2 cells were c...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(85)90389-8

    authors: Larrick JW,Brindley L,Doyle MV

    更新日期:1985-05-10 00:00:00

  • A humanised mouse model of cytokine release: comparison of CD3-specific antibody fragments.

    abstract::CD3-specific antibodies have shown clinical efficacy in both transplantation and autoimmunity. However, targeting CD3 in this way can lead to T-cell activation and a serious cytokine release syndrome mediated by Fcγ receptor binding. An in vivo mouse model has been developed using severe combined immunodeficient (SCID...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.07.001

    authors: Malcolm SL,Smith EL,Bourne T,Shaw S

    更新日期:2012-10-31 00:00:00

  • Isolating pure populations of monocytes from the blood of pregnant women: comparison of flotation in iodixanol with elutriation.

    abstract::Observations that the innate arm of the immune system is upregulated in pregnancy have highlighted the need for methods of isolating pure populations of monocytes for studies into pregnancy and pre-eclampsia without activating them during the isolation process. Density gradient centrifugation using iodixanol is a usef...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2004.05.011

    authors: Nutt JC,Willis CC,Morris JM,Gallery ED

    更新日期:2004-10-01 00:00:00

  • Construction of antibody mimics from a noncatalytic enzyme-detection of polysialic acid.

    abstract::We have used a conceptually novel way to construct antibody mimics based on the binding of a noncatalytic enzyme to its substrate. Bacteriophage-derived endosialidase cleaves polysialic acid (polySia), an important oncofetal and bacterial antigen, which is poorly immunogenic. We fused to green fluorescent protein (GFP...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2004.10.006

    authors: Jokilammi A,Ollikka P,Korja M,Jakobsson E,Loimaranta V,Haataja S,Hirvonen H,Finne J

    更新日期:2004-12-01 00:00:00

  • Creating a robust framework for the analysis of cryopreserved samples in quantitative immunological experiments.

    abstract::Longitudinal clinical or experimental immunological studies warrant the use of cryopreserved samples for flow cytometric phenotyping. Most notably CD62L+ and CD25+Foxp3+ counts were shown to be reduced by past studies. Here we are the first to compare the effects of cryopreservation on cell type calculations performed...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2013.03.006

    authors: Ogunjimi B,Hens N,Malfait R,Van Tendeloo V,Smits E

    更新日期:2013-06-28 00:00:00

  • Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector.

    abstract::We describe a stable chelating linkage for the reversible immobilization of oligohistidine tagged proteins in the flow cell of the 'BIAcore' surface plasmon resonance (SPR) biosensor. The carboxymethylated dextran surface of the flow cell was covalently derivatized with N-(5-amino-1-carboxypentyl)iminodiacetic acid (N...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(95)00032-6

    authors: Gershon PD,Khilko S

    更新日期:1995-06-14 00:00:00

  • Hematological response of rabbits to chronic, repetitive, severe bleedings for the production of antisera.

    abstract::A study was undertaken to determine whether large amounts of blood (antiserum) could be rapidly removed from rabbits over a two-month period using a simple suction technique. It was found that half a blood volume could be readily removed each week (over 900 ml/rabbit/54 days) without a single fatality. The rabbits eff...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(78)90081-9

    authors: Nerenberg ST,Zedler P,Prasad R,Biskup NS,Pedersen L

    更新日期:1978-01-01 00:00:00

  • Development of monoclonal antibodies and ELISA specific for the mouse vascular endocan.

    abstract::Human vascular endocan is a proteoglycan exhibiting tumorigenic activity through both its glycan and protein cores. Endocan mRNA is identified as being one of the most significant molecular signatures defining a poor prognosis in lung, breast, kidney, prostate, and thyroid malignancies. The survival inversely correlat...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.02.009

    authors: Depontieu F,de Freitas Caires N,Gourcerol D,Giordano J,Grigoriu B,Delehedde M,Lassalle P

    更新日期:2012-04-30 00:00:00

  • A method for the quantification of a colored or fluorescent signal in enzyme immunoassays by photodensitometry.

    abstract::A colored or fluorescent signal is generally evaluated with the naked eye, or by means of different more or less sophisticated and costly instruments. Photodensitometry is an additional technique which is both inexpensive and simple to perform. This technique can satisfactorily quantify a signal without the use of eit...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(87)90235-3

    authors: Labrousse H,Avrameas S

    更新日期:1987-10-23 00:00:00

  • Detection of eosinophil cationic protein (ECP) by an enzyme-linked immunosorbent assay.

    abstract::Eosinophil cationic protein (ECP) is a highly basic and potent cytotoxic single-chain zinc-containing protein present in the granules of the eosinophilic granulocytes. ECP appears to be involved in defence against parasites and in the tissue damage seen in subjects with allergic and inflammatory disease. To investigat...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(91)90177-h

    authors: Reimert CM,Venge P,Kharazmi A,Bendtzen K

    更新日期:1991-04-25 00:00:00

  • Cell-surface modification with an iodinatible imidoester to enhance radiolabeling.

    abstract::A procedure to enhance incorporation of radioactive iodine into cell-surface proteins is described. The effects of chemical modification of cells with the iodinatible haptens N-succinimidyl-3-(4-hydroxyphenyl) propionate (Bolton-Hunter reagent) and methyl-p-hydroxybenzimidate HC1 (Wood reagent) are compared. The Wood ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(85)90177-2

    authors: Wall KA,Fitch FW

    更新日期:1985-02-28 00:00:00

  • Isolation of neutrophil precursors from bone marrow for biochemical and transcriptional analysis.

    abstract::The neutrophilic granulocyte is the most numerous leukocyte in peripheral blood. The development from a multipotent progenitor cell to a mature neutrophil takes place in the bone marrow over a period of 10-14 days. In order to understand the cellular mechanisms behind this process, it is necessary to investigate cells...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0022-1759(99)00176-3

    authors: Cowland JB,Borregaard N

    更新日期:1999-12-17 00:00:00

  • Inductively coupled plasma mass spectrometry assay for quantification of free infliximab in serum.

    abstract::TNF antagonists such as infliximab are effective for the treatment of several inflammatory and autoimmune diseases. Recent clinical studies have advocated the importance of measuring trough infliximab levels to guide treatment decisions. We have developed a novel assay for measuring serum free infliximab levels using ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2019.04.008

    authors: Pavlov IY,Parker RL,Lázár-Molnár E,Strathmann FG,Delgado JC

    更新日期:2019-07-01 00:00:00