Abstract:
OBJECTIVE:To investigate the expression of peroxisome proliferator-activated receptors (PPAR)gamma and the effects of PPARgamma ligands on cells growth in colorectal cancer (CRC) cell line HT-29, and to explore whether the activation of PPARgamma by its selective ligand can induce apoptosis and the arrest of the cell cycle in these cells. METHODS:A CRC cell line, HT-29, was used in this study. PPARgamma mRNA and the protein expressions were measured by reverse transcriptase-polymerase chain reaction and Western blot. The HT-29 cells were treated with two specific PPARgamma ligands: rosiglitazone and 15-d-PGJ2. The effects of PPARgamma activated by rosiglitazone and 15-d-PGJ2 on the anchorage-dependent and anchorage-independent growth of the cells were assessed by methylthiazolyl terazolium (MTT) and soft agar colony assay, respectively. Apoptosis was measured by TUNEL staining and flow cytometry (FCM) assay by CaspSCREEN Flowcytometric Apoptosis Detection Kit (BioVision, Palo Alto, USA). Furthermore, the caspase-3 expression was determined by a immunocytochemical staining method before and after treatment with rosiglitazone and 15-d-PGJ2 for 48 h. The cell cycles were measured by flow cytometric analysis using propidium iodide (PI). RESULTS:PPARgamma mRNA and protein expressions were observed in the HT-29 cells. The MTT assay showed that treatment of these cells with 0, 0.1, 1 or 10 micromol/L PPARgamma activators rosiglitazone or 15-d-PGJ2 for 0, 24, 48 or 72 h resulted in the inhibition of anchorage-dependent cell growth in a dosage- and time-dependent way. Rosiglitazone treatment during cell growth resulted in the reduction of colony formation and the effects were not immediately reversible in the cell culture. TUNEL staining showed DNA fragmentation in positive cells after treatment with rosiglitazone and 15-d-PGJ2 for 48 h. In addition, FCM showed that the apoptosis rates were 14.8+/-0.8% and 28.5+/-1.3% or 15+/-0.7% and 40+/-1.2% after the cells were incubated with 10 micromol/L rosiglitazone or 15-d-PGJ2 for 24 h and 48 h, while the apoptosis rates of cells without treatment were 3.8+/-0.4% and 8.8+/-0.4%, respectively. Consistent with these results, the positivity rates of caspase-3 expression in cells treated with rosiglitazone or 15-d-PGJ2 increased significantly when compared with the control group. To explore whether the regulation of the cell cycle was involved in the effect of PPARgamma ligands on cell growth, FCM using PI staining was assessed. The ratio of G0/G1 phase cells increased after incubated with 10 micromol/L rosiglitazone or 15-d-PGJ2 for 24 h and 48 h. CONCLUSIONS:Our results showed that PPARgamma was expressed in HT-29 cells and PPARgamma activation could inhibit cell growth through inducing apoptosis and suppressing the cell cycle. PPARgamma may be considered as a new therapeutic target for colon cancer in humans.
journal_name
J Dig Disjournal_title
Journal of digestive diseasesauthors
Lin MS,Chen WC,Bai X,Wang YDdoi
10.1111/j.1443-9573.2007.00290.xsubject
Has Abstractpub_date
2007-05-01 00:00:00pages
82-8issue
2eissn
1751-2972issn
1751-2980pii
CDD290journal_volume
8pub_type
杂志文章abstract:OBJECTIVE:To investigate the role of ghrelin in the gastric cancer cell line AGS and its probable mechanism. METHODS:Cell proliferation was detected by MTT assay after treated with ghrelin or des-acyl ghrelin. The expression of growth hormone secretagogue receptor 1a (GHS-R1a) and 1b (GHS-R1b) mRNA was detected using ...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2012.00616.x
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVES:To investigate whether sending a family history questionnaire to patients prior to undergoing colonoscopy results in an increased availability of family history and better genetic counseling. METHODS:A questionnaire was mailed to patients before they underwent outpatient colonoscopy at a university hospital...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12491
更新日期:2017-06-01 00:00:00
abstract::Lgr5 marks stem cells in digestive epithelial tissues by lineage tracing, and in vitro ever-expansion of Lgr5 stem cells form organoids, which can be directed to differentiate into functional somatic cells. Organoids derived from gastrointestinal epithelium even recapitulate the morphologic features of their in vivo c...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12305
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the feasibility and accuracy of confocal laser endomicroscopy (CLE) for the in vivo diagnosis of colorectal cancer (CRC) compared with conventional histology. METHODS:Consecutive patients who had undergone CLE examination for screening or surveillance colonoscopy were recruited. Suspected maligna...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12039
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:The monocyte chemoattractant protein-1 (MCP-1) -A2518G gene polymorphism has been found to be involved in the susceptibility to inflammatory bowel disease (IBD); however, the results of existing studies are controversial. The aim of this meta-analysis was to assess the relationship between the MCP-1 -A2518G p...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,meta分析,评审
doi:10.1111/1751-2980.12232
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVES:To assess the prevalence and potential risk factors of latent tuberculosis infection (LTBI) in Chinese patients with inflammatory bowel disease (IBD) and to evaluate the role of chest computed tomography (CT) in the screening of LTBI. METHODS:A single-center retrospective study was conducted and all IBD pat...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12437
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:Systematic reports on acute upper gastrointestinal bleeding in children/adolescents are scanty. The aim of this study is to analyze its presentation, pathology and outcome in Hong Kong. METHODS:A retrospective review of the hospital database for admissions up to the age of 18 years with signs of acute upper ...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2008.00346.x
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:Hypomethylation within the body of the p53 gene, which is normally methylated, has been found in neoplasms. Also, the CG → TA transition was not detected in the CpG codons of the p53 gene in gastritis lesions in Iranian patients. Therefore, an evaluation of the probable correlation between global genome methy...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12053
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:This study aimed to determine the prevalence of and risk factors associated with elevated alanine aminotransferase (ALT) levels among a sample of normal weight, overweight and obese youth from two urban populations in Central Mexico. METHODS:Baseline data from 1262 youth aged 8-19 years who participated in t...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12072
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:Currently the screening and diagnosis of gastric cancer and atrophic gastritis are mainly made by endoscopy and biopsy. The aim of this study was to evaluate the use of serum tests: serum pepsinogen I (PGI pepsinogen I/II ratio (PGR), gastrin-17 (G-17) and H. pylori-immunoglobulin G (IgG) antibodies to screen...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1443-9573.2007.00271.x
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVES:Peptest is a new non-invasive reflux diagnostic test based on lateral flow technology that containing two highly specific human pepsin monoclonal antibodies for detecting pepsin, a biomarker for reflux disease. The primary aim of this multicenter clinical study was to validate the efficacy of Peptest in pati...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,多中心研究
doi:10.1111/1751-2980.12783
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:Contrast-enhanced harmonic endoscopic ultrasonography (CH-EUS) is a valuable device to diagnose and determine the malignant potential of gastrointestinal stromal tumors (GIST) as early as possible when making clinical therapeutic decisions. This study aimed to estimate the ability of CH-EUS to discriminate be...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,meta分析
doi:10.1111/1751-2980.12710
更新日期:2019-03-01 00:00:00
abstract::Helicobacter pylori (H. pylori) infection causes peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphomas and gastric adenocarcinomas, for which the pathogenesis of chronic gastric inflammation prevails and provides the pathogenic basis. Since the role of H. pylori infection is promoting carcinogenesis...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/j.1751-2980.2008.00334.x
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVE:We aimed to investigate the clinical features, precipitating factors, bowel habits, bothersome symptoms and treatment strategies of individuals in China with self-reported constipation. METHODS:An internet-based survey was conducted in China. The participants had self-reported chronic constipation (≥1 sympto...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12792
更新日期:2019-08-01 00:00:00
abstract::The incidence of early esophageal adenocarcinoma has been increasing significantly in recent decades. Prognosis depends greatly on the choice of treatment. Early cancers can be treated by endoscopic resection, whereas advanced carcinomas have to be sent for surgery. Esophageal resection is associated with high periope...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12264
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:This study aimed to compare the diagnostic value and image quality of high-definition magnifying white light endoscopy (M-WLE) and high-definition magnifying endoscopy with i-scan (M-i-scan) for Helicobacter pylori (H.pylori) infection. METHODS:Patients complaining of dyspepsia who underwent esophagogastrodu...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12086
更新日期:2013-11-01 00:00:00
abstract::Aldehyde dehydrogenase (ALDH2) and alcohol dehydrogenase (ADH) gene polymorphisms associating with enhanced acetaldehyde exposure and markedly increased cancer risk in alcohol drinkers provide undisputable evidence for acetaldehyde being a local carcinogen not only in esophageal but also in gastric cancer. Accordingly...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/j.1751-2980.2011.00480.x
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:Early diagnosis of colitis-associated cancer and dysplasia through surveillance endoscopy is vital for patients with ulcerative colitis (UC). This study aimed to evaluate the efficacy of autofluorescence endoscopy (AFE) using 5-aminolevulinic acid (ALA) and to investigate the fluorescence signal localization ...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12923
更新日期:2020-09-01 00:00:00
abstract::Fibroblast infiltration and collagen deposition result in structural changes in the bowel wall, and lead to strictures in intestinal inflammatory disease. While strictures can also occur in other contexts, such as malignancy, this review focuses on the surgical treatment of stricture secondary to inflammatory bowel di...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12880
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:The incidence of nonsteroidal anti-inflammatory drugs (NSAIDs)-induced enteropathy is currently increasing. However, the predictors of small bowel bleeding (SBB) associated with NSAIDs are unknown. This study aimed to assess the risk factors of SBB in chronic NSAIDs users. METHODS:We retrospectively compared...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12269
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:With an age-standardized incidence rate of 2 per 100 000, esophageal cancer is not common among Malaysians, but they are nevertheless important due to its poor prognosis. The study is to clarify whether the human papillomavirus (HPV) is associated with esophageal cancer in Malaysians as there has been no repo...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12605
更新日期:2018-05-01 00:00:00
abstract::Invasive gastric carcinoma is preceded by a cascade of precancerous lesions. The first recognized histologic change is active chronic inflammation, which may persist as such: non-atrophic chronic gastritis (no gland loss), or advance to multifocal atrophic gastritis (MAG), the first real step in the precancerous casca...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2011.00550.x
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To define the normative data of gastroesophageal reflux (GER) and laryngopharyngeal reflux (LPR) in healthy Chinese volunteers. METHODS:Healthy volunteers without gastrointestinal and throat disease and symptoms were enrolled and underwent 24-hour impedance-pH monitoring. RESULTS:A total of 37 healthy volun...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2011.00502.x
更新日期:2011-06-01 00:00:00
abstract::The development of alcoholic liver disease (ALD) is a complex process involving both the parenchymal and non-parenchymal cells in the liver. The impact of ethanol on hepatocytes can be characterized as a condition of organelle stress with multifactorial changes in hepatocellular function accumulating during ethanol ex...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/j.1751-2980.2010.00468.x
更新日期:2011-02-01 00:00:00
abstract::Gut microbiota constitute the largest reservoir of the human microbiome and are an abundant and stable ecosystem-based on its diversity, complexity, redundancy, and host interactions This ecosystem is indispensable for human development and health. The integrity of the intestinal mucosal barrier depends on its interac...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12849
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:Non-steroidal anti-inflammatory drugs (NSAID) induce gastroduodenal mucosal injury and are metabolized by cytochrome P450 2C9 (CYP2C9). It is postulated that CYP2C9 genotype is associated with NSAID-induced gastropathy. This study aims to determine whether individuals with a CYP2C9 allele mutation are suscept...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/j.1751-2980.2008.00326.x
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To explore the role of tagging single nucleotide polymorphisms (tagSNPs) in the adiponectin gene in the natural course of nonalcoholic fatty liver disease (NAFLD). METHODS:The participants were chosen from our previous survey containing 3543 individuals. Finally, a total of 696 participants who had been foll...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12288
更新日期:2015-10-01 00:00:00
abstract::Drug-induced liver injury (DILI), which is caused by drugs and herbal or dietary supplements, remains a serious concern for drug developers, regulators, and clinicians; however, serum biomarkers utilized to detect and monitor DILI have not changed in decades and have limitations. Data-driven mathematical modeling that...
journal_title:Journal of digestive diseases
pub_type: 杂志文章,评审
doi:10.1111/1751-2980.12684
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To investigate the clinicopathological features of columnar-lined esophagus (CLE) in Chinese patients. METHODS:Among 182 consecutive resections of proximal gastric cancer with residual esophageal tissue, 114 were eligible for the study. The maximal lengths of CLE and superficial esophageal glands (SEG) and t...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12007
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To determine whether computer-predicted short RNA structural analogues could inhibit hepatitis C virus (HCV) genotype 2a, 3a and 4a replication in cultured cells. METHODS:Short RNA sequences, X12, X12a and X12b, designed to be identical in secondary structure to the X region in the 3'-untranslated region (3'...
journal_title:Journal of digestive diseases
pub_type: 杂志文章
doi:10.1111/1751-2980.12250
更新日期:2015-08-01 00:00:00