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High-throughput screening of cellular features using high-resolution light-microscopy; application for profiling drug effects on cell adhesion.

Abstract：

:High-resolution light-microscopy and high-throughput screening are two essential methodologies for characterizing cellular phenotypes. Optimally combining these methodologies in cell-based screening to test detailed molecular and cellular responses to multiple perturbations constitutes a major challenge. Here we describe the development and application of a screening microscope platform that automatically acquires and interprets sub-micron resolution images at fast rates. The analysis pipeline is based on the quantification of multiple subcellular features and statistical comparisons of their distributions in treated vs. control cells. Using this platform, we screened 2200 natural extracts for their effects on the fine structure and organization of focal adhesions. This screen identified 15 effective extracts whose fractionation and characterization were further analyzed using the same microscope system. The significance of combining resolution, throughput and multi-parametric analyses for biomedical research and drug discovery is discussed.

journal_name

J Struct Biol

journal_title

Journal of structural biology

authors

Paran Y,Ilan M,Kashman Y,Goldstein S,Liron Y,Geiger B,Kam Z

doi

10.1016/j.jsb.2006.12.013

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

233-43

issue

2

eissn

1047-8477

issn

1095-8657

pii

S1047-8477(06)00390-X

journal_volume

158

pub_type

杂志文章

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