Abstract：
:We have established the DNA sequence and analyzed the transcription and translation products of a series of putative nodulation (nod) genes in Rhizobium meliloti strain 1021. Four loci have been designated nodF, nodE, nodG and nodH. The correlation of transposon insertion positions with phenotypes and open reading frames was confirmed by sequencing the insertion junctions of the transposons. The protein products of these nod genes were visualized by in vitro expression of cloned DNA segments in a R. meliloti transcription-translation system. In addition, the sequence for nodG was substantiated by creating translational fusions in all three reading frames at several points in the sequence; the resulting fusions were expressed in vitro in both E. coli and R. meliloti transcription-translation systems. A DNA segment bearing several open reading frames downstream of nodG corresponds to the putative nod gene mutated in strain nod-216. The transcription start sites of nodF and nodH were mapped by primer extension of RNA from cells induced with the plant flavone, luteolin. Initiation of transcription occurs approximately 25 bp downstream from the conserved sequence designated the "nod box," suggesting that this conserved sequence acts as an upstream regulator of inducible nod gene expression. Its distance from the transcription start site is more suggestive of an activator binding site rather than an RNA polymerase binding site.
journal_name
Geneticsjournal_title
Geneticsauthors
Fisher RF,Swanson JA,Mulligan JT,Long SRsubject
Has Abstractpub_date
1987-10-01 00:00:00pages
191-201issue
2eissn
0016-6731issn
1943-2631journal_volume
117pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Thirty-five nucleotide polymorphisms were found in a 21.5-kbp region including the Est6 locus among 42 isoallelic lines extracted from a single natural population of Drosophila melanogaster. The heterozygosity per nucleotide pair was estimated to be 0.010 overall, but was lower in sequences hybridizing to transcripts ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Stem cells have a fascinating biology and offer great prospects for therapeutic applications, stimulating intense research on what controls their properties and behavior. Although there have been significant advances in our understanding of how local microenvironments, or niches, control the maintenance and activity o...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.108.098244
更新日期：2008-12-01 00:00:00
abstract：:The endoplasmic reticulum (ER) is an extensive organelle in neurons with important roles at synapses including the regulation of cytosolic Ca2+, neurotransmission, lipid metabolism, and membrane trafficking. Despite intriguing evidence for these crucial functions, how the presynaptic ER influences synaptic physiology ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300261
更新日期：2017-11-01 00:00:00
abstract：:Fluorescent in situ hybridization was used to examine the distribution of six abundant long terminal repeat (LTR) retroelements, Opie, Huck, Cinful-1, Prem-2/Ji, Grande, and Tekay/Prem-1 on maize pachytene chromosomes. Retroelement staining in euchromatin was remarkably uniform, even when we included the structurally ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:There are two naturally occurring functional alleles of the recombination hotspot cog, which is located 3.5 kb from the his-3 locus of Neurospora crassa. The presence of the cog+ allele in a cross significantly increases recombination in the his-3 region compared to a cross homozygous for the cog allele. Data obtained...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.103.025080
更新日期：2004-07-01 00:00:00
abstract：:Efficient haplotyping in pedigrees is important for the fine mapping of quantitative trait locus (QTL) or complex disease genes. To reconstruct haplotypes efficiently for a large pedigree with a large number of linked loci, two algorithms based on conditional probabilities and likelihood computations are presented. Th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.103.021055
更新日期：2004-08-01 00:00:00
abstract：:The fission yeast Schizosaccharomyces pombe has never been found to give rise to viable cells totally lacking mitochondrial DNA (rho(o)). This paper describes the isolation of rho(o) strains of S. pombe by very long term incubation of cells in liquid medium containing glucose, potassium acetate and ethidium bromide. O...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Kinesin-1 is a motor protein that moves stepwise along microtubules by employing dimerized kinesin heavy chain (Khc) subunits that alternate cycles of microtubule binding, conformational change, and ATP hydrolysis. Mutations in the Drosophila Khc gene are known to cause distal paralysis and lethality preceded by the o...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.140798
更新日期：2012-09-01 00:00:00
abstract：:The process of close recombinant formation in bacteriophage T5 crosses has been studied by examining the structure of internal heterozygotes (HETs), the immediate products of recombination events. The T5 system was chosen because it permits the study of internal heterozygotes exclusively, thus avoiding the ambiguities...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:Transcriptional silencing involves the formation of specialized repressive chromatin structures. Previous studies have shown that the histone H3-H4 chaperone known as chromatin assembly factor 1 (CAF-1) contributes to transcriptional silencing in yeast, although the molecular basis for this was unknown. In this work w...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.054783
更新日期：2006-06-01 00:00:00
abstract：:We have studied a genome-wide set of single-nucleotide polymorphism (SNP) allele frequency measures for African-American, East Asian, and European-American samples. For this analysis we derived a simple, closed mathematical formulation for the spectrum of expected allele frequencies when the sampled populations have e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.1.351
更新日期：2004-01-01 00:00:00
abstract：:Transcriptional silencing in the budding yeast Saccharomyces cerevisiae may be linked to DNA replication and cell cycle progression. In this study, we have surveyed the effect of 41 mutations in genes with a role in replication, the cell cycle, and DNA repair on silencing at HMR. Mutations in PCNA (POL30), RF-C (CDC44...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:We study the mechanisms that guide the formation and maintenance of the highly ordered actin-myosin cytoskeleton in striated muscle. The UNC-82 kinase of Caenorhabditis elegans is orthologous to mammalian kinases ARK5/NUAK1 and SNARK/NUAK2. UNC-82 localizes to the M-line, and is required for proper organization of thi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.193029
更新日期：2017-03-01 00:00:00
abstract：:The adaptive character of inversion polymorphism in Drosophila subobscura is well established. The O(ST) and O(3+4) chromosomal arrangements of this species differ by two overlapping inversions that arose independently on O(3) chromosomes. Nucleotide variation in eight gene regions distributed along inversion O(3) was...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032748
更新日期：2005-03-01 00:00:00
abstract：:The replication of the rDNA complement of only one nucleolus organizer region during polytene chromosome formation (replicative dominance) was initially observed in Drosophila melanogaster. Here we demonstrate replicative dominance in Drosophila simulans and D. melanogaster/D. simulans interspecific hybrids. A second ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Amoebae of the Colonia (CL) strain, which normally produce plasmodia in clones, are shown to cross with mt3 and mt4 amoebae under appropriate conditions. Strains carrying mt3 and mt4 in a CL genetic background were constructed and used in the genetic analysis of mutants isolated in CL. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-12-01 00:00:00
abstract：:We analyzed the dynamics of transposable elements (TEs) according to Wright's island and continent-island models, assuming that selection tends to counter the deleterious effects of TEs. We showed that migration between host populations has no impact on either the existence or the stability of the TE copy number equil...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032243
更新日期：2005-01-01 00:00:00
abstract：:To identify genomic regions required for establishment and patterning of the epidermis, we screened 58 deficiencies that collectively delete at least approximately 67% of the Caenorhabditis elegans genome. The epidermal pattern of deficiency homozygous embryos was analyzed by examining expression of a marker specific ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:Understanding which genes contribute to evolutionary change and the nature of the alterations in them are fundamental challenges in evolution. We analyzed regulatory and enzymatic genes in the maize anthocyanin pathway as related to the evolution of anthocyanin-pigmented kernels in maize from colorless kernels of its ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The additive genomic variance in linear models with random marker effects can be defined as a random variable that is in accordance with classical quantitative genetics theory. Common approaches to estimate the genomic variance in random-effects linear models based on genomic marker data can be regarded as estimating ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302324
更新日期：2019-10-01 00:00:00
abstract：:The effective population size (N(e)) is notoriously difficult to accurately estimate in wild populations as it is influenced by a number of parameters that are difficult to delineate in natural systems. The different methods that are used to estimate N(e) are affected variously by different processes at the population...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.136580
更新日期：2012-06-01 00:00:00
abstract：:Genetic and developmental analysis of an X-linked vital locus vnd was undertaken. Embryos hemizygous for the original allele vnd did not hatch and exhibited a disorganized ventral nervous system (VNS). The mutation maps in the region 1B6-7 to 1B9-10, a subregion of an area previously shown to be essential to normal ne...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The S-M checkpoint ensures that entry into mitosis is dependent on completion of DNA replication. In the fission yeast Schizosaccharomyces pombe, the SM checkpoint mutant cdc2-3w is thought to be defective in receiving the checkpoint signal. To isolate genes that function in the checkpoint pathway, we screened an S. p...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:In this article we investigate multiplicative effects between genes in relation to heterosis. The extensive literature on heterosis due to multiplicative effects between characters is reviewed, as is earlier work on the genetic description of heterosis. A two-locus diallelic model of arbitrary gene action is used to d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The HML and HMR loci carry unexpressed copies of MATa and MAT alpha information, and a replica of that information is transposed to MAT during mating-type interchange in Saccharomyces yeasts. A negative control mechanism keeps silent the information located at the HML and HMR loci. We mapped these loci by constructing...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:There are two distinct reasons for making comparisons of genetic variation for quantitative characters. The first is to compare evolvabilities, or ability to respond to selection, and the second is to make inferences about the forces that maintain genetic variability. Measures of variation that are standardized by the...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:The genetics of the ciliate Tetrahymena thermophila are richer than for most other eukaryotic cells, because Tetrahymena possesses two genomes: a germline (micronuclear) genome that follows a Mendelian model of genetic transmission and a somatic (macronuclear) genome, derived from the micronuclear genome by fragmentat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Two large experiments were conducted in order to evaluate the heterozygous effects of irradiated chromosomes on viability. Mutations were accumulated on several hundred second chromosomes by delivering doses of 2,500r over either two or four generations for total X-ray exposures of 5,000r or 10,000r. Chromosomes treat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:There is increasing recognition that genetic diversity can affect the spread of diseases, potentially affecting plant and livestock disease control as well as the emergence of human disease outbreaks. Nevertheless, even though computational tools can guide the control of infectious diseases, few epidemiological models...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.179853
更新日期：2015-11-01 00:00:00
abstract：:Analyses of 113 putative Mutator-induced events involving the yg2 locus of chromosome 9 revealed that 11 of these events were deletions that produce albino seedlings when homozygous. This phenotype is characteristic of wd (white deficiency) deletions. All 11 wd-Mu deletions failed to complement wd1 and pyd1 (pale-yell...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
