Abstract:
:Three hundred and fifteen kilobases of DNA from the rosy-Ace region on chromosome 3R of D. melanogaster have previously been cloned and extensively characterized. We describe the isolation of nine new deficiency mutants that break within the 315-kb interval. The position of these breakpoints on the DNA map was determined by in situ and Southern hybridization. Further, we more precisely mapped the breakpoints of several deletions previously analyzed. The results permit us to delimit sequences essential to the known complementation groups in the region within approximately 20 kb in most cases. However, one gene, B16-1, is shown to contain essential sequences that span about 50 kb. Also, we demonstrate by overlapping deficiencies that a 45-kb DNA segment from the region, which includes one known complementation group, allows limited survival when deleted.
journal_name
Geneticsjournal_title
Geneticsauthors
Gausz J,Hall LM,Spierer A,Spierer Psubject
Has Abstractpub_date
1986-01-01 00:00:00pages
65-78issue
1eissn
0016-6731issn
1943-2631journal_volume
112pub_type
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