Rapid staining for the sperm penetration assay using a bisbenzimide dye.

Abstract:

:Our rapid staining method, accomplished by adding the vital fluorochrome bisbenzimide (Hoechst 33342) directly into the sperm droplet before fertilization, eases visual interpretation of sperm penetration and decondensation. For both within- and between-experiment comparisons, inclusion of bisbenzimide did not influence the overall SPA score or the progress of sperm-egg interaction. These results suggest that bisbenzimide can be used routinely to facilitate scoring of the SPA.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Bean B,Fogle TA,Gates RD

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

214-6

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)54088-6

journal_volume

55

pub_type

杂志文章
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  • In vitro maturation (IVM) of murine and human germinal vesicle (GV)-stage oocytes by coculture with immortalized human fallopian tube epithelial cells.

    abstract:OBJECTIVE:To improve the maturation rate of murine and human germinal vesicle (GV) oocytes using human tubal epithelial cells (hTECs). DESIGN:Murine and human GV oocytes were randomized to human tubal fluid (HTF) media alone or cocultured with mouse embryonic fibroblasts (MEFs) or primary hTECs or immortalized hTECS (...

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    更新日期:2011-03-15 00:00:00

  • The clinical value of salpingoscopy in tubal infertility.

    abstract::A specially designed rigid salpingoscope has been developed to allow inspection of the tubal mucosa during laparoscopy. The presence and extent of intratubal adhesion formation can be evaluated more accurately by this technique than by either HSG or laparoscopy. In 22 patients with bilateral hydrosalpinges, an intraut...

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    doi:10.1016/s0015-0282(16)60501-0

    authors: De Bruyne F,Puttemans P,Boeckx W,Brosens I

    更新日期:1989-02-01 00:00:00

  • The use of the argon laser in the treatment of experimental endometriosis.

    abstract::The argon laser offers several theoretical advantages over excision, electrocautery, or CO2 laser therapy for the treatment of peritoneal implants of endometriosis. Therefore, a study of the feasibility of the argon laser for the treatment of endometriosis was performed using five virgin New Zealand White rabbits. Fol...

    journal_title:Fertility and sterility

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    doi:10.1016/s0015-0282(16)46752-x

    authors: Keye WR Jr,Matson GA,Dixon J

    更新日期:1983-01-01 00:00:00

  • Sustained intrauterine release of levonorgestrel over five years.

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    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0015-0282(16)49397-0

    authors: Nilsson CG,Lahteenmaki PL,Luukkainen T,Robertson DN

    更新日期:1986-06-01 00:00:00

  • Twice-frozen embryos are no detriment to pregnancy success: a retrospective comparative study.

    abstract:OBJECTIVE:To compare the pregnancy rates (PR) and live birth rates in once- versus twice-frozen ET treatment cycles in the same cohort of women. DESIGN:A retrospective study. SETTING:Fertility clinics, IVF Australia, New South Wales. PATIENT(S):The study population was all women who underwent thawing of twice-frozen...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2011.04.034

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    更新日期:2011-07-01 00:00:00

  • Obesity and poor reproductive outcome: the potential role of the endometrium.

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    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2006.11.162

    authors: Bellver J,Melo MA,Bosch E,Serra V,Remohí J,Pellicer A

    更新日期:2007-08-01 00:00:00

  • Daily levels of salivary progesterone during menstrual cycle in adolescent girls.

    abstract:OBJECTIVE:To study the daily levels of salivary progesterone (P) during the menstrual cycles of adolescent girls 14 to 19 years of age and the occurrence of anovulations and defective luteal phases. DESIGN:The salivary P levels were analyzed by radioimmunoassay from three age groups of girls (n = 65) and compared with...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:

    authors: Vuorento T,Huhtaniemi I

    更新日期:1992-10-01 00:00:00

  • Electroejaculation before chemotherapy in adolescents and young men with cancer.

    abstract:OBJECTIVE:To evaluate the outcome of repeated electroejaculation for obtaining semen from adolescents and young men before initiation of anticancer therapies. DESIGN:Retrospective clinical study. SETTING:Bikur Cholim Hospital, Jerusalem, Israel. PATIENT(S):Six young male patients (average age, 18+/-3 years) with dia...

    journal_title:Fertility and sterility

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    doi:10.1016/s0015-0282(01)01680-6

    authors: Hovav Y,Dan-Goor M,Yaffe H,Almagor M

    更新日期:2001-04-01 00:00:00

  • Embryonic adhesion is not affected by endometrial leptin receptor gene silencing.

    abstract:OBJECTIVE:In rodents, evidence suggests that the leptin system is mandatory for embryonic implantation. We aimed to investigate the functional relevance of the endometrial leptin receptor (OB-R) in the adhesion phase of human implantation. DESIGN:We used an in vitro model for embryonic adhesion, composed of a human en...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2006.12.028

    authors: Cervero A,Domínguez F,Horcajadas JA,Quiñonero A,Pellicer A,Simón C

    更新日期:2007-10-01 00:00:00

  • The use and effectiveness of in vitro fertilization in women with endometriosis: the surgeon's perspective.

    abstract:OBJECTIVE:To assess the use and effectiveness of IVF in a cohort of women undergoing surgery for endometriosis. DESIGN:Cohort study. SETTING:University hospital. PATIENT(S):Four hundred thirty-eight patients who attempted to become pregnant after conservative surgery for endometriosis. INTERVENTION(S):Interview. M...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2008.02.004

    authors: Somigliana E,Daguati R,Vercellini P,Barbara G,Benaglia L,Crosignani PG

    更新日期:2009-05-01 00:00:00

  • Child with Beckwith-Wiedemann syndrome born after assisted reproductive techniques to an human immunodeficiency virus serodiscordant couple.

    abstract:OBJECTIVE:To report a child with Beckwith-Wiedemann syndrome (BWS) born after assisted reproductive technology (ART) to an HIV serodiscordant couple. DESIGN:Case report. SETTING:Academic medical center. PATIENT(S):A child with BWS born after ART to an HIV serodiscordant couple. INTERVENTION(S):Assisted reproductive...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2011.04.030

    authors: Kuentz P,Bailly A,Faure AC,Blagosklonov O,Amiot C,Bresson JL,Roux C

    更新日期:2011-07-01 00:00:00

  • Searching for the infertile man with hyperprolactinemia.

    abstract::The purpose of this study was to determine whether routine prolactin measurement was of use when investigating men with infertility. Prolactin levels were slightly higher in men with primary infertility, compared with men with secondary infertility and a fertile control group. Although this slight increase was statist...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)45862-0

    authors: Hargreave TB,Richmond JD,Liakatas J,Elton RA,Brown NS

    更新日期:1981-11-01 00:00:00

  • Prevalence and heterogeneity of antiphosphatidylethanolamine antibodies in patients with recurrent early pregnancy losses.

    abstract:OBJECTIVE:To describe the prevalence of antiphospholipid antibodies to both anionic and zwitterionic phospholipids in women with early recurrent pregnancy losses (RPLs). DESIGN:Retrospective data analysis. SETTING:Tokai University Hospital, Kanagawa, Japan. PATIENT(S):One hundred thirty-nine patients with unexplaine...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(99)00119-3

    authors: Sugi T,Katsunuma J,Izumi S,McIntyre JA,Makino T

    更新日期:1999-06-01 00:00:00

  • Retroperitoneal mobilization of the vas deferens in the complex vasovasostomy.

    abstract::Anatomic distances along retroperitoneal, inguinal, and infrainguinal segments of the vas deferens were measured in 14 formalin fixed cadavers and in 15 recently postmortem males. There were no significant differences in segment lengths between the two groups nor between the right and left vasa. Data from the recent p...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)53959-4

    authors: Buch JP,Woods T

    更新日期:1990-11-01 00:00:00

  • Morphologic abnormalities in 2-year-old children born after in vitro fertilization/intracytoplasmic sperm injection with preimplantation genetic screening: follow-up of a randomized controlled trial.

    abstract:OBJECTIVE:To evaluate the effect of preimplantation genetic screening (PGS) on morphologic outcome in children. DESIGN:Follow-up of a randomized controlled trial (RCT). SETTING:University hospital. PATIENT(S):Two-year-old children born to mothers who participated in an RCT on the efficacy of PGS: 50 children born af...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.fertnstert.2012.10.024

    authors: Beukers F,van der Heide M,Middelburg KJ,Cobben JM,Mastenbroek S,Breur R,van der Lee JH,Hadders-Algra M,Bos AF,Kok JH,PGS Study Group.

    更新日期:2013-02-01 00:00:00

  • Complete evaluation of anatomy and morphology of the infertile patient in a single visit; the modern infertility pelvic ultrasound examination.

    abstract::The comprehensive "one-stop shop" ultrasound evaluation of an infertile woman, performed around cycle days 5 to 9, will reveal abundant information about the anatomy and morphology of the pelvic organs and thereby avoid costly radiation and iodinated contrast exposure. We propose a two-dimensional and three-dimensiona...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审

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    更新日期:2016-06-01 00:00:00

  • Over a decade of experience with preimplantation genetic diagnosis: a multicenter report.

    abstract:OBJECTIVE:To review a 12-year experience of the world's three largest preimplantation genetic diagnosis (PGD) centers. DESIGN:Multicenter analysis of the clinical outcome of PGD. SETTING:In vitro fertilization programs at the Reproductive Genetics Institute, Chicago, Illinois; Saint Barnabas Medical Center, West Oran...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2003.09.082

    authors: Verlinsky Y,Cohen J,Munne S,Gianaroli L,Simpson JL,Ferraretti AP,Kuliev A

    更新日期:2004-08-01 00:00:00

  • Live birth is the correct outcome for clinical trials evaluating therapy for the infertile couple.

    abstract::Well-designed and -conducted clinical trials are needed to further advance the field for reproductive medicine. However, current reporting of outcomes of trials is ambiguous and disparate. In this review it is offered that the preferred outcome for clinical trials in reproductive medicine should be live birth. Multipl...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审

    doi:10.1016/j.fertnstert.2014.03.026

    authors: Barnhart KT

    更新日期:2014-05-01 00:00:00

  • Opposing viscerovisceral effects of surgically induced endometriosis and a control abdominal surgery on the rat bladder.

    abstract:OBJECTIVE:To determine, in rats, how surgically induced endometriosis and a control surgery (partial hysterectomy; sutures in abdomen) affects micturition thresholds and bladder vascular permeability. DESIGN:Two animal studies, each performed in three groups of urethane-anesthetized rats in proestrus. SETTING:Academi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2006.03.026

    authors: Morrison TC,Dmitrieva N,Winnard KP,Berkley KJ

    更新日期:2006-10-01 00:00:00

  • Avoiding ovarian hyperstimulation syndrome with the use of gonadotropin-releasing hormone agonist trigger.

    abstract::Ovarian hyperstimulation syndrome (OHSS) is one of the most serious, and potentially lethal, complications of controlled ovarian stimulation (COS). Induction of final oocyte maturation with a bolus of gonadotropin-releasing hormone (GnRH) agonist (GnRHa), instead of the criterion standard hCG, in patients undergoing o...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审

    doi:10.1016/j.fertnstert.2015.02.004

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  • Why are multiple pregnancy rates and single embryo transfer rates so different globally, and what do we do about it?

    abstract::In the early years of in vitro fertilization, overall pregnancy rates were low, and it was considered necessary to transfer more than one embryo to increase the chances of pregnancy. It was not until advances in assisted reproductive technologies resulting in increased pregnancy rates that the concept of transferring ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审

    doi:10.1016/j.fertnstert.2020.09.003

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  • The heparin-glutathione test: an alternative to the hypo-osmotic swelling test to select viable sperm for intracytoplasmic sperm injection.

    abstract:OBJECTIVE:To evaluate the heparin-glutathione test (HEGLUT) for the selection of viable sperm for intracytoplasmic sperm injection (ICSI). DESIGN:A prospective study. SETTING:Department of Pediatrics, Obstetrics and Gynecology, University of Valencia and Instituto Valenciano de Infertilidad. PATIENT(S):Semen samples...

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    pub_type: 杂志文章

    doi:10.1016/s0015-0282(98)00334-3

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  • Cyclic changes in the mammary gland of cynomolgus macaques.

    abstract:OBJECTIVE:To evaluate the influence of hormonal changes during the menstrual cycle on the mammary gland of female cynomolgus monkeys. DESIGN:Paired breast biopsy samples were obtained during the follicular and luteal phases of the cycle. Cycle characteristics were assessed by vaginal bleeding, serum hormones, vaginal ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2004.04.035

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    更新日期:2004-10-01 00:00:00

  • Increase in nerve fibers and loss of mast cells in polycystic and postmenopausal ovaries.

    abstract:OBJECTIVE:To quantify nerve fibers and mast cells in human ovaries at different functional stages. DESIGN:Retrospective study. SETTING:Research laboratory of the university. SPECIMEN(S): 8 human ovaries in the follicular (cyclic) phase, 7 polycystic ovaries, and postmenopausal ovaries with (n=5) or without (n=7) hype...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/s0015-0282(01)01805-2

    authors: Heider U,Pedal I,Spanel-Borowski K

    更新日期:2001-06-01 00:00:00

  • Evaluation of endometrial activin A secretion for prediction of pregnancy after intrauterine insemination.

    abstract:OBJECTIVE:To measure activin A concentrations in uterine washing fluid collected from women with couple infertility undergoing intrauterine insemination (IUI). DESIGN:Retrospective case-control study. SETTING:Tertiary university center for women's care. PATIENT(S):Fifty women, of whom 25 became pregnant after up to ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章

    doi:10.1016/j.fertnstert.2008.12.125

    authors: Florio P,Bruni L,Galleri L,Reis FM,Borges LE,Bocchi C,Litta P,De Leo V,Petraglia F

    更新日期:2010-05-01 00:00:00

  • Occult hyperprolactinemia in infertile women.

    abstract:OBJECTIVE:To define the hypersensitive status of PRL secretion in normoprolactinemic infertile women and determine the incidence of occult hyperprolactinemia among them. DESIGN:The potential for PRL secretion was examined in 463 women. SETTING:Outpatient clinic in a university hospital. PATIENTS:Three hundred sixty-...

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  • Does a woman's educational attainment influence in vitro fertilization outcomes?

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    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.fertnstert.2011.05.015

    authors: Mahalingaiah S,Berry KF,Hornstein MD,Cramer DW,Missmer SA

    更新日期:2011-06-30 00:00:00

  • Effect of caffeine on increasing the motility of frozen human sperm.

    abstract::A preliminary report on the effect of the addition of caffeine to 45 frozen sperm samples, in order to restore the effectiveness and motility of frozen stored human sperm, is presented. The addition of 7.2 mM caffeine proved optimal and resulted in 40% to 80% increased sperm motility after 30 days of frozen storage. T...

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    pub_type: 杂志文章

    doi:10.1016/s0015-0282(16)42378-2

    authors: Barkay J,Zuckerman H,Sklan D,Gordon S

    更新日期:1977-02-01 00:00:00

  • Prevalence of hyperandrogenemia among nonhirsute oligo-ovulatory women.

    abstract:OBJECTIVE:To test the hypothesis that oligo-ovulatory ovulation is associated with hyperandrogenemia in the absence of other clinical signs of androgen excess. DESIGN:Retrospective cohort controlled. SETTING:Tertiary care university-based outpatient clinic. PATIENT(S):Thirty-two consecutive nonhirsute oligo-ovulator...

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    pub_type: 杂志文章

    doi:10.1016/s0015-0282(97)80089-1

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    更新日期:1997-03-01 00:00:00