Abstract:
OBJECTIVE:In rodents, evidence suggests that the leptin system is mandatory for embryonic implantation. We aimed to investigate the functional relevance of the endometrial leptin receptor (OB-R) in the adhesion phase of human implantation. DESIGN:We used an in vitro model for embryonic adhesion, composed of a human endometrial cell line (HEC1-A) and B6C3F1 mouse embryos. The OB-R gene was silenced in a stable manner by RNA interference, and embryonic adhesion rates were analyzed. SETTING:Research laboratory at a university-affiliated center. INTERVENTION(S):RNA interference. MAIN OUTCOME MEASURE(S):Embryonic adhesion in cells treated with OB-R RNAi. RESULT(S):The OB-R shRNA-transfected cells exhibited up to 80% lower OB-R mRNA levels than those of cells nontransfected or transfected with scrambled shRNA. The OB-R protein was also highly diminished in the stable OB-R shRNA-transfected HEC1A cells, whereas OAS1 expression was similar in both nontransfected and transfected cells. Embryonic adhesion rate was similar in nontransfected (94%) and HEC1-A transfected cells with a scrambled sequence (94%) or with OB-R silencing sequence (92%). CONCLUSION(S):Knocking down the OB-R gene in a human endometrial cell line has no effect on the embryonic adhesion rate. Nevertheless, the functional relevance of this system can not be excluded in other phases of embryonic implantation, such as the invasion phase. Moreover, we describe a new approach to the functional analysis of candidate molecules implicated in blastocyst adhesion.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Cervero A,Domínguez F,Horcajadas JA,Quiñonero A,Pellicer A,Simón Cdoi
10.1016/j.fertnstert.2006.12.028subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1086-92issue
4 Suppleissn
0015-0282issn
1556-5653pii
S0015-0282(07)00056-8journal_volume
88pub_type
杂志文章abstract:OBJECTIVE:To identify if there are certain cutoff levels for P and or the P/E(2) ratio on the day of hCG that would be defined as detrimental for occurrence of pregnancy in women with normal ovarian reserve undergoing cleavage-stage embryo transfer (ET). Secondarily, to determine if these same cutoffs might have the sa...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.12.065
更新日期:2011-04-01 00:00:00
abstract::The result of the hormone concentration of one blood sample was used to determine the accuracy of predicting not only the hormone concentrations of a second and third sample drawn 15 minutes apart, but also the mean value of the three samples. Three blood specimens from 73 men involved in two previously reported studi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59662-9
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:To investigate the expression and localization of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) in women with and without endometriosis. DESIGN:Comparative immunohistochemical study. SETTING:Academic medical center. PATIENT(S):Ectopic (n = 24) and homologous eutopic endometrium (n = 24) f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.12.067
更新日期:2009-03-01 00:00:00
abstract::Reducing exposure to toxic environmental agents is a critical area of intervention for obstetricians, gynecologists, and other reproductive health care professionals. Patient exposure to toxic environmental chemicals and other stressors is ubiquitous, and preconception and prenatal exposure to toxic environmental agen...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.08.043
更新日期:2013-10-01 00:00:00
abstract::Fifty patients have been sterilized laparoscopically using a plastic tubal (Bleier) clip having a "snap-shut" mechanism. Initial assessment indicates that the method is simple in principle but there are limitations, including the bulky dimensions of the applicator, absence of a safety-catch mechanism, and faults in th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44111-7
更新日期:1979-07-01 00:00:00
abstract::Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of immunohistochemical profiles of estrogen receptor-alpha and P receptor corroborate previously published results from the use of conventional immunohistochemistry, thus validating TMA use in endome...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.06.044
更新日期:2004-12-01 00:00:00
abstract::The proportions of good, fair, and poor embryos in 13 women with bilateral endometriomas were compared with those of 39 women without endometriomas and were found to be similar (47.2% vs. 41.1%, 28.3% vs. 32.8%, and 24.3% vs. 26.0%, respectively). Therefore, it appears that the presence of bilateral endometriomas duri...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.03.002
更新日期:2011-06-30 00:00:00
abstract::Microsurgical nonvascularized transplantation of a 1-cm segment of fallopian tube was performed using the rabbit as a model. Autograft experiments showed survival of a 1-cm tubal segment for as long as 10 months postoperatively. Allografts survived equally as well, and one rabbit became pregnant. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45691-8
更新日期:1981-08-01 00:00:00
abstract:OBJECTIVE:To determine the concentrations of angiopoietin 1 (ANGPT1) and ANGPT2 in individual human preovulatory follicles in relation to their diameter or volume to clarify the role of these molecules in folliculogenesis. DESIGN:Prospective study. SETTING:Research laboratory at Kansai Medical University, Osaka, Japa...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2011.09.031
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker. DESIGN:Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro. SETTING:Research laboratory of a university hospit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57803-0
更新日期:1995-09-01 00:00:00
abstract::To examine pregnancy rate in patients undergoing controlled ovarian hyperstimulation (COH) with use of multidose GnRH antagonists in multiple repeated IVF cycle attempts, we conducted a survey in 785 consecutive IVF cycles in our unit. Although pregnancy rate significantly decreased beyond the second IVF cycle attempt...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.07.1725
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVE:To determine whether patients who failed an in vitro fertilization (IVF) cycle can proceed with a subsequent IVF cycle after waiting only one menstrual cycle, or whether there is a benefit to allowing two or more menstrual cycles to elapse before proceeding. DESIGN:Retrospective cohort study. SETTING:Academ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.11.044
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:To evaluate whether some ultrasound parameters of ovarian morphology can discriminate between control women and patients with polycystic ovary syndrome (PCOS). DESIGN:Retrospective data analysis. SETTING:Volunteers women in an academic research environment. PATIENT(S):Eighty amenorrheic or oligomenorrheic ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01919-7
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVE:To determine the efficacy and safety of Repronex SC as compared with Repronex IM and Pergonal IM in patients undergoing ovulation induction. DESIGN:Randomized, open-label, multicenter, parallel group study. SETTING:Ten academic and private fertility clinics with expertise in ovualtion induction. PATIENT(S)...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0015-0282(01)01856-8
更新日期:2001-07-01 00:00:00
abstract::The role of insulin sensitizing agents is discussed at length in this Committee Opinion. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2008.08.004
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To evaluate correlations between oxygen consumption (OC) measurements before and after embryo cytokinesis, observing OC during embryo cleavages and combining that information with morphokinetics to relate to implantation potential. DESIGN:Prospective cohort study. SETTING:University-affiliated private IVF u...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.03.019
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:To define the hypersensitive status of PRL secretion in normoprolactinemic infertile women and determine the incidence of occult hyperprolactinemia among them. DESIGN:The potential for PRL secretion was examined in 463 women. SETTING:Outpatient clinic in a university hospital. PATIENTS:Three hundred sixty-...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56154-8
更新日期:1993-09-01 00:00:00
abstract:OBJECTIVE:To determine, for a woman aged >45 years, whether selected hormonal, health status, and demographic measures are related to the time to final menstrual period (FMP) from a point where 6 weeks separate the shortest and longest cycles experienced to date. DESIGN:Cohort study. SETTING:Volunteers in an academic...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03231-4
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVE:To investigate for differences in reproductive hormone levels in male relatives of women with polycystic ovary syndrome (PCOS). DESIGN:Cross-sectional study. SETTING:Academic medical center. PATIENT(S):Sixty-three fathers and 66 brothers of women with PCOS as well as two groups of control men of comparable...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.03.029
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:Assisted hatching may enhance embryo implantation. This study was conducted to examine the efficacy of the laser- and chemical-assisted hatching for promotion of implantation (IR), pregnancy (PR), and delivery rate (DR) in older women undergoing IVF cycles. DESIGN:Prospective study. SETTING:An IVF unit of a...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)03172-2
更新日期:2002-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of the environmental estrogens polychlorinated biphenyls (PCBs) and phthalate esters (PEs) as potential environmental hazards in the deterioration of semen parameters in infertile men without an obvious etiology. DESIGN:Randomized controlled study. SETTING:Tertiary care referral inferti...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)04389-3
更新日期:2002-12-01 00:00:00
abstract:OBJECTIVE:We evaluated the association between subfertility and low birth weight infants. DESIGN:Women in this analysis participated in a cross-sectional investigation of maternal risk factors for adverse pregnancy outcomes. SETTING:Medical and obstetric history and life style information were obtained from women who...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54597-x
更新日期:1991-10-01 00:00:00
abstract:OBJECTIVE:To determine whether there is a relationship between prewash total motile count and live births in couples undergoing IUI. DESIGN:Retrospective review in a single academic center. SETTING:Not applicable. PATIENT(S):Couples with infertility undergoing ovulation induction with IUI between 2010 and 2014. INT...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.12.025
更新日期:2019-04-01 00:00:00
abstract::Two models of levonorgestrel-releasing intrauterine devices were studied. Model A was designed to deliver 20 micrograms/day, and model B was designed to deliver 30 micrograms/day. Plasma concentrations of levonorgestrel were determined in blood samples taken from women who used the levonorgestrel-releasing intrauterin...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)49397-0
更新日期:1986-06-01 00:00:00
abstract:OBJECTIVE:To determine whether retinoic acid (RA) can regulate the expression of interleukin (IL)-6 in human endometrial cells in a manner that might be beneficial to women with endometriosis. DESIGN:In vitro study. SETTING:Academic medical center. PATIENT(S):Patients with endometriosis and controls. INTERVENTION(S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00483-0
更新日期:2000-05-01 00:00:00
abstract::Anaphylactic reaction associated with hysteroscopy using 32% dextran-70 as a distending medium is a rare phenomenon. We report anaphylactic reaction associated with hysteroscopy using dextran that was delivered manually in three otherwise healthy women occurring within a 6-month period. The rapidity and pressure of de...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54317-9
更新日期:1991-05-01 00:00:00
abstract:OBJECTIVE:To compare the levonorgestrel intrauterine system (LNG-IUS) with hysterectomy in patients with adenomyosis and to study the effects of both treatments on quality of life (QOL). DESIGN:Prospective randomized clinical trial. SETTING:Women's health teaching and research hospital. PATIENT(S):Eighty-six patient...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.10.009
更新日期:2011-02-01 00:00:00
abstract::A case of a genital tuberculosis found during laparoscopic evaluation for recurrent acute pelvic pain is presented. The symptoms of genital tuberculosis can vary greatly, and a laparoscopic approach is effective for making the diagnosis. Clinicians should consider tuberculosis as a differential diagnosis when encounte...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.09.063
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2006.05.072
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:To investigate whether antitrophoblast antibodies are associated with unexplained recurrent miscarriages, we used choriocarcinoma cells JEG-3, since these cells are negative for class I and II antigens, but they do express HLA-G, resembling an antigen expression of endovascular and interstitial trophoblasts. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.11.014
更新日期:2012-02-01 00:00:00