Abstract:
:There is a wealth of knowledge in the field of in vitro diagnostics with regard to preanalytical variables and their impact on the determination of peptide and protein analytes in human serum and plasma. This information is applicable to clinical proteomics investigations, which utilize the same sample types. Studies have demonstrated that the majority of variations and errors in in vitro diagnostics seem to occur in the preanalytical phase prior to specimen analysis. Preanalytical processes include study design, compliance of the subjects investigated, compliance of the technical staff in adherence to protocols, choice of specimens utilized and sample collection and processing. These variables can have a dramatic impact on the determination of analytes and can affect result outcomes, reproducibility and the validity of investigations. By drawing analogies to in vitro diagnostics practices, specific variables that are likely to impact the results of proteomics studies can be identified. Recognition of such variables is the first step towards their understanding and, eventually, controlling their impact. In this article, we will review preanalytical variables, provide examples for their effects on the determination of distinct peptides and proteins and discuss potential implications for clinical proteomics investigations.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Rai AJ,Vitzthum Fdoi
10.1586/14789450.3.4.409subject
Has Abstractpub_date
2006-08-01 00:00:00pages
409-26issue
4eissn
1478-9450issn
1744-8387journal_volume
3pub_type
杂志文章,评审abstract:INTRODUCTION:Leptomeningeal metastases (LM) from lymphoma remain a difficult complication for oncologist due to the high incidence in morbidity and mortality. Early diagnostic and initiation of treatment are essential to prevent neurological deterioration. Areas covered: In this review, several proteomic approaches are...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1307106
更新日期:2017-04-01 00:00:00
abstract::Ovarian cancer is the fifth leading cause of cancer deaths among North American women. Regrettably, there is currently no reliable circulating biomarker that can detect ovarian cancer in its early stages. The CA125 biomarker is very useful for treatment response monitoring, but its sensitivity is very low for early de...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.1.121
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abstract::Proteomic technologies have experienced major improvements in recent years. Such advances have facilitated the discovery of potential tumor markers with improved sensitivities and specificities for the diagnosis, prognosis and treatment monitoring of cancer patients. This review will focus on four state-of-the-art pro...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.1
更新日期:2009-04-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
abstract::Huntington's disease (HD) is caused by expansion of a polyglutamine repeat in the N-terminal region of huntingtin (htt), a large protein that has been found to interact with a variety of proteins. It remains to be determined how the interactions of htt with other proteins are involved in the pathogenesis of HD. A rece...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2006-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2015.1069188
更新日期:2015-01-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2019-09-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2012-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2018-11-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.68
更新日期:2009-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2014.859527
更新日期:2014-02-01 00:00:00
abstract:INTRODUCTION:Lung cancer and related diseases have been one of the most common causes of deaths worldwide. Genomic-based biomarkers may hardly reflect the underlying dynamic molecular mechanism of functional protein interactions, which is the center of a disease. Recent developments in mass spectrometry (MS) have made ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1304215
更新日期:2017-04-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.6.799
更新日期:2008-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2015.1099436
更新日期:2015-01-01 00:00:00
abstract:INTRODUCTION:Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers worldwide. However, there remain many unmet clinical needs, from diagnosis to treatment strategies. The inherent complexity of the molecular characteristics of PDAC has made it difficult to meet these challenges, rendering proteomic pr...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1382356
更新日期:2017-11-01 00:00:00
abstract::Due to the enormous complexity of the proteome, focus in proteomics shifts more and more from the study of the complete proteome to the targeted analysis of part of the proteome. The isolation of this specific part of the proteome generally includes an affinity-based enrichment. Surface plasmon resonance (SPR), a labe...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.3.425
更新日期:2008-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2019.1646128
更新日期:2019-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.5.705
更新日期:2008-10-01 00:00:00
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更新日期:2020-02-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.11.64
更新日期:2011-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.858021
更新日期:2013-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
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journal_title:Expert review of proteomics
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更新日期:2018-02-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2011-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
abstract::The 3rd International Conference on Proteomics & Bioinformatics (Proteomics 2013) Philadelphia, PA, USA, 15-17 July 2013 The Third International Conference on Proteomics & Bioinformatics (Proteomics 2013) was sponsored by the OMICS group and was organized in order to strengthen the future of proteomics science by brin...
journal_title:Expert review of proteomics
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更新日期:2013-10-01 00:00:00
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journal_title:Expert review of proteomics
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