Abstract:
:Due to the enormous complexity of the proteome, focus in proteomics shifts more and more from the study of the complete proteome to the targeted analysis of part of the proteome. The isolation of this specific part of the proteome generally includes an affinity-based enrichment. Surface plasmon resonance (SPR), a label-free technique able to follow enrichment in real-time and in a semiquantitative manner, is an emerging tool for targeted affinity enrichment. Furthermore, in combination with mass spectrometry (MS), SPR can be used to both selectively enrich for and identify proteins from a complex sample. Here we illustrate the use of SPR-MS to solve proteomics-based research questions, describing applications that use very different types of immobilized components: such as small (drug or messenger) molecules, peptides, DNA and proteins. We evaluate the current possibilities and limitations and discuss the future developments of the SPR-MS technique.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Visser NF,Heck AJdoi
10.1586/14789450.5.3.425subject
Has Abstractpub_date
2008-06-01 00:00:00pages
425-33issue
3eissn
1478-9450issn
1744-8387journal_volume
5pub_type
杂志文章,评审abstract::Ubiquitin-proteasome-mediated protein degradation is central to the regulation of many important biological processes, including cell cycle progression, apoptosis and DNA repair. Recognition and degradation of ubiquitinated substrates by the 26S proteasome is tightly regulated to maintain normal cell growth. Disruptio...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.5.649
更新日期:2007-10-01 00:00:00
abstract::Osteoporosis is prevalent among the elderly and is a major cause of bone fracture in this population. Bone integrity is maintained by the dynamic processes of bone resorption and bone formation (bone remodeling). Osteoporosis results when there is an imbalance of the two counteracting processes. Bone mineral density, ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/epr.09.90
更新日期:2010-02-01 00:00:00
abstract:INTRODUCTION:An accurate diagnostic classification of thyroid lesions remains an important clinical aspect that needs to be addressed in order to avoid 'diagnostic' thyroidectomies. Among the several 'omics' techniques, proteomics is playing a pivotal role in the search for diagnostic markers. In recent years, differen...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1532793
更新日期:2018-11-01 00:00:00
abstract::Proteomics is performed in microgravity research in order to determine protein alterations occurring qualitatively and quantitatively, when single cells or whole organisms are exposed to real or simulated microgravity. To this purpose, antibody-dependent (Western blotting, flow cytometry, Luminex(®) technology) and an...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2014.926221
更新日期:2014-08-01 00:00:00
abstract:INTRODUCTION:Mass spectrometry (MS) is widely used in the characterization of biomolecules including peptide and protein therapeutics. These biotechnology products have seen rapid growth over the past few decades and continue to dominate the global pharmaceutical market. Advances in MS instrumentation and techniques ha...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1469982
更新日期:2018-05-01 00:00:00
abstract::Introduction: Metaproteomics is an established method to obtain a comprehensive taxonomic and functional view of microbial communities. After more than a decade, we are now able to describe the promise, reality, and perspectives of metaproteomics and provide useful information about the choice of method, applications,...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1738931
更新日期:2020-02-01 00:00:00
abstract::Bile is a body fluid produced by the liver and drained by biliary ducts into the duodenum. It has two major functions: first, it contains bile acids, which are critical for the digestion of fats, and second, it is an excretory pathway for many endogenous and exogenous compounds. Proteomic analysis of bile is particula...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.12
更新日期:2009-06-01 00:00:00
abstract::Clinical proteomics has been applied to the identification of biomarkers of obstetric and neonatal disease. We will discuss a number of encouraging studies that have led to potentially valid biomarkers in the context of Down's syndrome, preterm birth, amniotic infections, preeclampsia, intrauterine growth restriction ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2014.872564
更新日期:2014-02-01 00:00:00
abstract::Baukje de Roos is a principal investigator at the University of Aberdeen, Rowett Institute of Nutrition and Health. She investigates mechanisms through which dietary fats and fatty acids, and also polyphenols, affect parameters involved in the development of heart disease in vivo. This is achieved not only by measurin...
journal_title:Expert review of proteomics
pub_type: 面试
doi:10.1586/epr.09.52
更新日期:2009-08-01 00:00:00
abstract::Prenatal diagnosis is now offered to high-risk pregnancies, including advanced maternal age, ultrasound anomalies and positive Down's syndrome screening, and karyotype on cultured fetal material is the test of choice to screen these pregnancies. However, microscope analysis can only detect gross chromosome abnormaliti...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.13.9
更新日期:2013-04-01 00:00:00
abstract:INTRODUCTION:Mass spectrometry (MS)-based proteomics has become an indispensable tool for the characterization of the proteome and its post-translational modifications (PTM). In addition to standard protein sequence databases, proteogenomics strategies search the spectral data against the theoretical spectra obtained f...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1352474
更新日期:2017-08-01 00:00:00
abstract::Introduction: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has entered clinical diagnostics and is today a generally accepted and integral part of the workflow for microbial identification. MALDI-TOF MS identification systems received approval from national and internatio...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2019.1645603
更新日期:2019-08-01 00:00:00
abstract::Evaluation of: Di Girolamo F, Boschetti E, Chung MC, Guadagni F, Righetti PG. 'Proteomineering' or not? The debate on biomarker discovery in sera continues. J. Proteomics 74(5), 589-594 (2011). The combinatorial peptide ligand library in association with mass spectrometry can greatly enhance the dynamic range of the a...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.11.43
更新日期:2011-08-01 00:00:00
abstract::Protein profiling using high-throughput tandem mass spectrometry has become a powerful method for analyzing changes in global protein expression patterns in cells and tissues as a function of developmental, physiologic and disease processes. This review summarizes the utility and practical application of multidimensio...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.1.27
更新日期:2005-01-01 00:00:00
abstract::Human saliva is an attractive body fluid for disease diagnosis and prognosis because saliva testing is simple, safe, low-cost and noninvasive. Comprehensive analysis and identification of the proteomic content in human whole and ductal saliva will not only contribute to the understanding of oral health and disease pat...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.4.531
更新日期:2007-08-01 00:00:00
abstract:INTRODUCTION:Cutaneous leishmaniasis (CL), caused by different Leishmania parasite species, is associated with parasite-induced immune-mediated skin inflammation and ulceration. Whereas many CL studies focus on gene expression signatures in mouse models, the transcriptional response driving human patients in the field ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1812390
更新日期:2020-01-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in females of reproductive age, and its prevalence ranges between 6 and 8%. Associated problems include infertility, menstrual disorders, hirsutism and obesity. In addition, individuals with PCOS may be at increased risk of diabetes, endometrial ca...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.68
更新日期:2009-10-01 00:00:00
abstract::The 3rd International Conference on Proteomics & Bioinformatics (Proteomics 2013) Philadelphia, PA, USA, 15-17 July 2013 The Third International Conference on Proteomics & Bioinformatics (Proteomics 2013) was sponsored by the OMICS group and was organized in order to strengthen the future of proteomics science by brin...
journal_title:Expert review of proteomics
pub_type:
doi:10.1586/14789450.2013.842899
更新日期:2013-10-01 00:00:00
abstract::Huntington's disease (HD) is caused by expansion of a polyglutamine repeat in the N-terminal region of huntingtin (htt), a large protein that has been found to interact with a variety of proteins. It remains to be determined how the interactions of htt with other proteins are involved in the pathogenesis of HD. A rece...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.11.72
更新日期:2012-01-01 00:00:00
abstract::Viruses have coevolved with their hosts, acquiring strategies to subvert host cellular pathways for effective viral replication and spread. Human cytomegalovirus (HCMV), a widely-spread β-herpesvirus, is a major cause of birth defects and opportunistic infections in HIV-1/AIDS patients. HCMV displays an intricate syst...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2014.971116
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:Lung cancer and related diseases have been one of the most common causes of deaths worldwide. Genomic-based biomarkers may hardly reflect the underlying dynamic molecular mechanism of functional protein interactions, which is the center of a disease. Recent developments in mass spectrometry (MS) have made ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1304215
更新日期:2017-04-01 00:00:00
abstract::Type II diabetes increases the risk for cognitive decline via multiple traits. Amylin is a pancreatic hormone that has amyloidogenic and cytotoxic properties similar to the amyloid-β peptide. The amylin hormone is overexpressed in individuals with pre-diabetic insulin resistance or obesity leading to amylin oligomeriz...
journal_title:Expert review of proteomics
pub_type: 社论,评审
doi:10.1586/14789450.2015.1104251
更新日期:2015-01-01 00:00:00
abstract::It is well known that chronic, excessive consumption of alcohol can cause brain damage/structural changes in the regions important for neurocognitive function. Some of the damages are permanent, while others are reversible. Molecular mechanisms underlying alcohol-induced and/or -related brain damage are largely unknow...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.2.321
更新日期:2008-04-01 00:00:00
abstract::Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. However, very little is known about the regulation of SC maintenance pathways in cancer and ho...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.24
更新日期:2012-06-01 00:00:00
abstract::Globally, colorectal cancer (CRC) is the third most common malignant neoplasm. However, highly sensitive, specific, noninvasive tests that allow CRC diagnosis at an early stage are still needed. As circulatory blood reflects the physiological status of an individual and/or the disease status for several disorders, eff...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2014.905743
更新日期:2014-08-01 00:00:00
abstract:INTRODUCTION:The mission of the Chromosome-Centric Human Proteome Project (C-HPP), is to map and annotate the entire predicted human protein set (~20,000 proteins) encoded by each chromosome. The initial steps of the project are focused on 'missing proteins (MPs)', which lacked documented evidence for existence at prot...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1394189
更新日期:2017-12-01 00:00:00
abstract::High-throughput proteomics technologies tend to provide highly sensitive information about living tissues and biological fluids. Analytes are characterized by intrinsic and extrinsic properties, the latter depending on each phase of their preparation, sometimes adding artifacts with crucial repercussions in result rel...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.3.363
更新日期:2007-06-01 00:00:00
abstract:INTRODUCTION:The application of new proteomics methods may help to identify new diagnostic/predictive molecular markers in an attempt to improve the clinical management of atherosclerosis. Areas covered: Technological advances in proteomics have enhanced its sensitivity and multiplexing capacity, as well as the possibi...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1353912
更新日期:2017-08-01 00:00:00
abstract::Introduction: Autophagy is one of the most conserved clearance systems through which eukaryotes manage to handle dysfunctional and excess organelles and macromolecules. This catabolic process has not only a role in the maintenance of basal turnover of cellular components, but it is also essential in cells adaptation t...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2019.1691919
更新日期:2019-01-01 00:00:00
abstract:INTRODUCTION:Extracellular vesicles are emerging sources of biomarkers for modern preventive and precision medicine. Extracellular vesicles in body fluids offer a unique opportunity for integrative biomarker approaches due to their complex biocargo that includes proteins, lipids, nucleic acids and metabolites. Mass spe...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1392244
更新日期:2017-12-01 00:00:00