Depolarization of polymorphonuclear leukocytes by Porphyromonas (Bacteroides) gingivalis 381 in the absence of respiratory burst activation.


:Bacteroides spp. may contribute to the chronicity of mixed infections by affecting the normal functions of polymorphonuclear leukocytes (PMN). This study evaluated the physiologic and biochemical responses of human peripheral blood PMN to a variety of strains of the oral periodontal pathogen Porphyromonas (Bacteroides) gingivalis. Strain 381 and ATCC type strain 33277 caused rapid and lasting depolarization of the electrochemical potential that exists across the PMN membrane by a mechanism that was independent of activation of the pertussis toxin-sensitive G protein or protein kinase C. Membrane depolarization did not initiate increases in intracellular calcium or respiratory burst activation, and activity was not inhibited by surface proteolysis or sugars. However, membrane depolarization was associated with inhibition of PMN responses to the chemotactic peptide N-formylmethionyl leucyl phenylalanine. Membrane-depolarizing activity was isolated with the outer membrane of strain 381 by surface extraction of the bacteria by using Zwittergent 3,14, followed by Sephacryl S-200 gel filtration chromatography. The partially purified outer membrane components were heat stable, were not inhibited by tosyl-lysine chloromethyl ketone, and inhibited N-formylmethionyl leucyl phenylalanine-stimulated superoxide production. The results suggest that outer membrane components of P. gingivalis 381 and 33277 have porinlike activity that can depolarize PMN membranes and immobilize PMN responses to chemotactic peptides. This may prove to be an important virulence characteristic of these strains.


Infect Immun


Infection and immunity


Novak MJ,Cohen HJ





Has Abstract


1991-09-01 00:00:00












  • Apoptosis of renal cortical cells in the hemolytic-uremic syndrome: in vivo and in vitro studies.

    abstract::This study examined apoptotic cell death associated with Shiga-like toxin (Stx)-producing Escherichia coli. Renal cortices from three children with postenteropathic hemolytic-uremic syndrome (HUS) and from mice infected with E. coli O157:H7 and pediatric renal tubular epithelial cells stimulated with Stx and E. coli O...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Karpman D,Håkansson A,Perez MT,Isaksson C,Carlemalm E,Caprioli A,Svanborg C

    更新日期:1998-02-01 00:00:00

  • Natural immune response to the C-terminal 19-kilodalton domain of Plasmodium falciparum merozoite surface protein 1.

    abstract::We have characterized the natural immune responses to the 19-kDa domain of merozoite surface protein 1 in individuals from an area of western Kenya in which malaria is holoendemic. We used the three known natural variant forms of the yeast-expressed recombinant 19-kDa fragment that are referred to as the E-KNG, Q-KNG,...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shi YP,Sayed U,Qari SH,Roberts JM,Udhayakumar V,Oloo AJ,Hawley WA,Kaslow DC,Nahlen BL,Lal AA

    更新日期:1996-07-01 00:00:00

  • Limited role of secreted aspartyl proteinases Sap1 to Sap6 in Candida albicans virulence and host immune response in murine hematogenously disseminated candidiasis.

    abstract::Candida albicans secreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence of sap-nul...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Correia A,Lermann U,Teixeira L,Cerca F,Botelho S,da Costa RM,Sampaio P,Gärtner F,Morschhäuser J,Vilanova M,Pais C

    更新日期:2010-11-01 00:00:00

  • Molecular analysis of the serotyping antigens of Neisseria meningitidis.

    abstract::Molecular approaches to the rapid analysis of the serotyping antigens of Neisseria meningitidis, the class 2 and 3 outer membrane proteins (OMPs), were developed, evaluated, and used to study 12 antigenic variants of these proteins. A primer set for the polymerase chain reaction (PCR) amplification of the genes encodi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Feavers IM,Suker J,McKenna AJ,Heath AB,Maiden MC

    更新日期:1992-09-01 00:00:00

  • Type-specific antigens of group B type Ic streptococci.

    abstract::The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serological and chemical methods. The Ia antigen, shared by types Ia and Ic, is a polysaccharide composed of 69% galactose and 25% glucosamine (i.e., 31% N-acetyl-glucosamine). However, t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wilkinson HW,Eagon RG

    更新日期:1971-11-01 00:00:00

  • Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans.

    abstract::Previous studies suggested that nontypeable Haemophilus influenzae (NTHI) can form biofilms during human and chinchilla middle ear infections. Microscopic analysis of a 5-day biofilm of NTHI strain 2019 grown in a continuous-flow chamber revealed that the biofilm had a diffuse matrix interlaced with multiple water cha...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Greiner LL,Watanabe H,Phillips NJ,Shao J,Morgan A,Zaleski A,Gibson BW,Apicella MA

    更新日期:2004-07-01 00:00:00

  • Impaired bone resorption by lipopolysaccharide in vivo in mice deficient in the prostaglandin E receptor EP4 subtype.

    abstract::In a previous study we showed that the involvement of EP4 subtype of the prostaglandin E (PGE) receptor is crucial for lipopolysaccharide (LPS)-induced osteoclast formation in vitro. The present study was undertaken to test whether EP4 is actually associated with LPS-induced bone resorption in vivo. In wild-type (WT) ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sakuma Y,Tanaka K,Suda M,Komatsu Y,Yasoda A,Miura M,Ozasa A,Narumiya S,Sugimoto Y,Ichikawa A,Ushikubi F,Nakao K

    更新日期:2000-12-01 00:00:00

  • Structural similarities among malaria toxins insulin second messengers, and bacterial endotoxin.

    abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Caro HN,Sheikh NA,Taverne J,Playfair JH,Rademacher TW

    更新日期:1996-08-01 00:00:00

  • Mannan-binding lectin enhances susceptibility to visceral leishmaniasis.

    abstract::Levels of the serum opsonin mannan-binding lectin (MBL) were directly correlated with the probability of developing visceral leishmaniasis. Monocytes infected with MBL-opsonized Leishmania chagasi promastigotes secreted higher levels of tumor necrosis factor alpha and interleukin-6 than cells infected with nonopsonize...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Santos IK,Costa CH,Krieger H,Feitosa MF,Zurakowski D,Fardin B,Gomes RB,Weiner DL,Harn DA,Ezekowitz RA,Epstein JE

    更新日期:2001-08-01 00:00:00

  • Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms.

    abstract::Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous ba...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Berg RD,Savage DC

    更新日期:1975-02-01 00:00:00

  • The Bordetella pertussis type III secretion system tip complex protein Bsp22 is not a protective antigen and fails to elicit serum antibody responses during infection of humans and mice.

    abstract::The type III secretion system (T3SS) of pathogenic bordetellae employs a self-associating tip complex protein Bsp22. This protein is immunogenic during infections by Bordetella bronchiseptica and could be used as a protective antigen to immunize mice against B. bronchiseptica challenge. Since low-passage clinical isol...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Villarino Romero R,Bibova I,Cerny O,Vecerek B,Wald T,Benada O,Zavadilova J,Osicka R,Sebo P

    更新日期:2013-08-01 00:00:00

  • Borrelia burgdorferi spirochetes induce mast cell activation and cytokine release.

    abstract::The Lyme disease spirochete, Borrelia burgdorferi, is introduced into human hosts via tick bites. Among the cell types present in the skin which may initially contact spirochetes are mast cells. Since spirochetes are known to activate a variety of cell types in vitro, we tested whether B. burgdorferi spirochetes could...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Talkington J,Nickell SP

    更新日期:1999-03-01 00:00:00

  • Iron limitation and the gamma interferon-mediated antihistoplasma state of murine macrophages.

    abstract::The zoopathogenic fungus Histoplasma capsulatum requires iron for growth. Intracellular growth of the fungus within mouse peritoneal macrophages is inhibited by recombinant murine gamma interferon (IFN-gamma). Such treatment of mouse peritoneal macrophages induces a marked downshift in transferrin receptors. We tested...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lane TE,Wu-Hsieh BA,Howard DH

    更新日期:1991-07-01 00:00:00

  • Role of gamma-delta T cells in murine Chlamydia trachomatis infection.

    abstract::The role of gamma-delta T cells in host resistance to Chlamydia trachomatis was characterized by using a murine model of pneumonia caused by the mouse pneumonitis agent (MoPn), murine C. trachomatis. At days 3 and 7 after infection, gamma-delta T-cell-deficient knockout mice had significantly higher levels of MoPn in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Williams DM,Grubbs BG,Kelly K,Pack E,Rank RG

    更新日期:1996-09-01 00:00:00

  • Endoplasmic Reticulum Tubule Protein Reticulon 4 Associates with the Legionella pneumophila Vacuole and with Translocated Substrate Ceg9.

    abstract::Intracellular growth of Legionella pneumophila occurs in a replication vacuole constructed by host proteins that regulate vesicular traffic from the host endoplasmic reticulum (ER). This process is promoted by a combination of approximately 300 Icm/Dot translocated substrates (IDTS). One of these proteins, Ceg9, was p...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Haenssler E,Ramabhadran V,Murphy CS,Heidtman MI,Isberg RR

    更新日期:2015-09-01 00:00:00

  • Isolation, characterization, and inhibition kinetics of enolase from Streptococcus rattus FA-1.

    abstract::One aspect in a broad spectrum of possible mechanisms of cariostatic reactions of fluoride is its interaction with the metabolism of oral bacteria. Information on the mechanisms and kinetics of fluoride inhibition of essential enzymes of the glycolytic pathway of the relevant bacteria is lacking. In this work, the iso...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hüther FJ,Psarros N,Duschner H

    更新日期:1990-04-01 00:00:00

  • Escherichia coli heat-stable enterotoxin: rapid method of purification and some characteristics of the toxin.

    abstract::In this study we used Escherichia coli strain F11(P155) of porcine origin. The heat-stable enterotoxin (ST) was produced with a batch fermentor under agitation (500 rpm) and forced aeration (5 liters/min) in Casamino Acids yeast extract medium containing 0.2% glucose. The pH varied from 7.2 to 7.8. The maximum amount ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lallier R,Lariviere S,St-Pierre S

    更新日期:1980-05-01 00:00:00

  • The acylated form of protein D of Haemophilus influenzae is more immunogenic than the nonacylated form and elicits an adjuvant effect when it is used as a carrier conjugated to polyribosyl ribitol phosphate.

    abstract::The nonacylated form of protein D (PDm) of Haemophilus influenzae has been shown to induce the production of antibodies that are bactericidal to homologous and heterologous nontypeable H. influenzae (NTHi) strains. In this study, immunization of rats with lipoprotein D (LPD) induced higher levels of anti-protein D imm...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Akkoyunlu M,Melhus A,Capiau C,van Opstal O,Forsgren A

    更新日期:1997-12-01 00:00:00

  • Class II major histocompatibility complex molecule expression on murine eosinophils activated in vivo by Brugia malayi.

    abstract::The Th-2 response to microfilariae of Brugia malayi in BALB/c mice is associated with peritoneal eosinophilia. These eosinophils had an activated hypodense phenotype in vivo and expressed high levels of the class II major histocompatibility complex (MHC). Interleukin 4 up-regulated the class II MHC, whereas gamma inte...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mawhorter SD,Pearlman E,Kazura JW,Boom WH

    更新日期:1993-12-01 00:00:00

  • Experimental syphilis and serological examination for treponematosis in hares.

    abstract::Of 202 captive hares studied, many of which had lesions on their external genital organs or testicular atrophy or both, 27% had positive serological tests for syphilis although dark-field examination of extracts of atrophic testes was negative. A total of 12 hares that were nonreactive for the serological test for syp...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Horvath I,Kemenes F,Molnar L,Szeky A,Racz I

    更新日期:1980-01-01 00:00:00

  • Comparative genomic analysis using microarray demonstrates a strong correlation between the presence of the 80-kilobase pathogenicity island and pathogenicity in Kanagawa phenomenon-positive Vibrio parahaemolyticus strains.

    abstract::Vibrio parahaemolyticus is a gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Almost all of the clinical V. parahaemolyticus isolates exhibit beta-type hemolysis on Wagatsuma agar, known as the Kanagawa phenomenon (KP). KP is induced by the thermostable dire...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Izutsu K,Kurokawa K,Tashiro K,Kuhara S,Hayashi T,Honda T,Iida T

    更新日期:2008-03-01 00:00:00

  • Genetic diversity among Escherichia coli O157:H7 isolates and identification of genes linked to human infections.

    abstract::An Escherichia coli oligonucleotide microarray based on three sequenced genomes was validated for comparative genomic microarray hybridization and used to study the diversity of E. coli O157 isolates from human infections and food and animal sources. Among 26 test strains, 24 (including both Shiga toxin [Stx]-positive...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wu G,Carter B,Mafura M,Liebana E,Woodward MJ,Anjum MF

    更新日期:2008-02-01 00:00:00

  • Colonization of the large bowel by Clostridium difficile in healthy infants: quantitative study.

    abstract::Colonization of the large bowel of healthy infants by Clostridium difficile was studied. Feces were collected from five breast-fed aand five formula-fed infants throughout the first year of life, and levels of C. difficile were quantitated. Three breast-fed and five formula-fed infants were colonized for periods of be...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Stark PL,Lee A,Parsonage BD

    更新日期:1982-03-01 00:00:00

  • Association between the tumor necrosis factor locus and the clinical outcome of Leishmania chagasi infection.

    abstract::A periurban outbreak of visceral leishmaniasis (VL) caused by the protozoan Leishmania chagasi is ongoing outside Natal, northeast Brazil. Manifestations range from asymptomatic infection to disseminated visceral disease. Literature reports suggest that both genetic and environmental factors influence the outcome of i...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Karplus TM,Jeronimo SM,Chang H,Helms BK,Burns TL,Murray JC,Mitchell AA,Pugh EW,Braz RF,Bezerra FL,Wilson ME

    更新日期:2002-12-01 00:00:00

  • Variable adherence of fimbriated Haemophilus influenzae type b to human cells.

    abstract::The attachment of isogenic fimbriated and nonfimbriated Haemophilus influenzae type b variants to human cells was studied by using a radioactive assay and an indirect immunofluorescent assay. As described previously, fimbriated H. influenzae variants adhered to a greater extent than nonfimbriated variants to human buc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sable NS,Connor EM,Hall CB,Loeb MR

    更新日期:1985-04-01 00:00:00

  • Identification of a novel sialic acid transporter in Haemophilus ducreyi.

    abstract::Haemophilus ducreyi, the causative agent of chancroid, produces a lipooligosaccharide (LOS) which terminates in N-acetyllactosamine. This glycoform can be further extended by the addition of a single sialic acid residue to the terminal galactose moiety. H. ducreyi does not synthesize sialic acid, which must be acquire...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Post DM,Mungur R,Gibson BW,Munson RS Jr

    更新日期:2005-10-01 00:00:00

  • Importance of antibodies to lipopolysaccharide in natural and vaccine-induced serum bactericidal activity against Neisseria meningitidis group B.

    abstract::Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Schmiel DH,Moran EE,Keiser PB,Brandt BL,Zollinger WD

    更新日期:2011-10-01 00:00:00

  • Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modification.

    abstract::Burkholderia pseudomallei is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS ty...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wikraiphat C,Saiprom N,Tandhavanant S,Heiss C,Azadi P,Wongsuvan G,Tuanyok A,Holden MT,Burtnick MN,Brett PJ,Peacock SJ,Chantratita N

    更新日期:2015-05-01 00:00:00

  • Immunity to chlamydial infections of the eye. II. Studies of passively transferred serum antibody in resistance to infection with guinea pig inclusion conjunctivitis.

    abstract::Conjunctival infection of guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis confers immunity. However, the mechanism of resistance to this intracellular pathogen is not yet defined. In the study reported here, serum immunoglobulin was passively transferred with resultant titers in excess of th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Watson RR,Mull JD,MacDonald AB,Thompson SE 3rd,Bear SE

    更新日期:1973-04-01 00:00:00

  • Interactions between Yersinia enterocolitica and rabbit ileal mucus: growth, adhesion, penetration, and subsequent changes in surface hydrophobicity and ability to adhere to ileal brush border membrane vesicles.

    abstract::Interactions between Yersinia enterocolitica and rabbit ileal mucus were examined. Strains carrying the Yersinia virulence plasmid, pYV, adhered to crude mucus but not to intestinal luminal contents that had been immobilized on polystyrene. Using an Y. enterocolitica O:9 mutant in which the yadA gene (formerly called ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Paerregaard A,Espersen F,Jensen OM,Skurnik M

    更新日期:1991-01-01 00:00:00