Abstract:
:O6-alkylguanine-DNA alkyltransferase (MGMT) represents the first line of defense against the toxic, mutagenic and carcinogenic effects of O6-alkylguanine adducts in DNA. These adducts mediate the biological activity from a series of alkylating agents, such as the tobacco-specific nitrosamines, believed to contribute to the carcinogenicity of tobacco smoke. There have been conflicting reports on the effects of smoking on MGMT activity in lung and other tissues. Here, we investigate MGMT activity in peripheral blood mononuclear cells (PBMC) and lung bronchial epithelial cells (BEC), extracted by lung brushings, from smokers and nonsmokers attending a bronchoscopy clinic. MGMT activity was significantly lower in BECs (geometric mean; 95% confidence interval 1.02; 0.86-1.20 fmol/microg DNA) than in PBMCs (7.86; 6.70-9.59 fmol/microg DNA; p < 0.001), suggesting that bronchial epithelia may be particularly sensitive to alkylation damage. More importantly our results indicate that activity in BECs is significantly decreased in samples from current smokers (0.71; 0.54-0.93 fmol/microg DNA) compared to nonsmokers (1.25; 1.03-1.51 fmol/microg DNA; p = 0.002). This could represent an important contribution to the carcinogenicity of tobacco smoke.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Povey AC,O'Donnell P,Barber P,Watson M,Margison GP,Santibáñez Koref MFdoi
10.1002/ijc.21790keywords:
subject
Has Abstractpub_date
2006-07-15 00:00:00pages
463-6issue
2eissn
0020-7136issn
1097-0215journal_volume
119pub_type
杂志文章abstract::History of diabetes may be associated with decreased prostate cancer (PCa) risk. Published studies have not always accounted for time since diabetes diagnosis or confounding and effect modification by lifestyle factors. The authors investigated the relationship between diabetes and PCa risk in men in the Health Profes...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24044
更新日期:2009-03-15 00:00:00
abstract::Dickkopf1 (DKK1), a secreted inhibitor of the Wnt/β-catenin pathway, is a negative regulator of bone formation. DKK1 acts as a switch that transitions prostate cancer bone metastases from osteolytic to osteoblastic and also is an active indicator of poor outcome for multiple myeloma. However, in other tumor types, DKK...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.26449
更新日期:2012-04-01 00:00:00
abstract::The relation between breast feeding and breast cancer was investigated in a multicentric case-control study conducted in Italy on 2,167 parous women with histologically confirmed breast cancer, diagnosed within 1 year, and 2,208 parous control women admitted to hospitals in the same catchment areas of cases for acute,...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/(SICI)1097-0215(19960717)67:2<161::AID-IJC
更新日期:1996-07-17 00:00:00
abstract::Combining the specificity of tumor-targeting bacteria with the sensitivity of biomarker detection would create a screening method able to detect small tumors and metastases. To create this system, we genetically modified an attenuated strain of Salmonella enterica to release a recombinant fluorescent biomarker (or flu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32414
更新日期:2020-01-01 00:00:00
abstract::Cytokines such as Interleukin (IL)-12p70 ("IL-12") and IL-23 can influence tumor progression. We tested the hypothesis that blood levels of IL-12p40, the common subunit of both cytokines, are associated with melanoma progression. Blood from 2,048 white melanoma patients were collected at a single institution between M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29182
更新日期:2015-04-15 00:00:00
abstract::For patients with brain tumors identification of diagnostic and prognostic markers in easy accessible biological material, such as plasma or cerebrospinal fluid (CSF), would greatly facilitate patient management. MIC-1/GDF15 (growth differentiation factor 15) is a secreted protein of the TGF-beta superfamily and emerg...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24639
更新日期:2009-12-01 00:00:00
abstract::Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase overexpressed in tumors, which plays a major role in the first step of pro-MMP-2 activation, leading to the generation of an intermediate 62 kDa species. The second step of MMP-2 activation that yields to the mature form is less understoo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10134
更新日期:2002-03-01 00:00:00
abstract::In neuroblastoma (NB), genetic alterations in chromatin remodeling (CRGs) and epigenetic modifier genes (EMGs) have been described. We sought to determine their frequency and clinical impact. Whole exome (WES)/whole genome sequencing (WGS) data and targeted sequencing (TSCA®) of exonic regions of 33 CRGs/EMGs were ana...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32361
更新日期:2019-11-15 00:00:00
abstract::Breast cancer is the cancer diagnosed most frequently in women worldwide. In Europe it is the most common cancer in the female population, with approximately 350,000 new cases diagnosed each year including 130,000 deaths. Incidence rates are increasing in the majority of European countries, whereas a decline in mortal...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20514
更新日期:2004-12-20 00:00:00
abstract::Human papillomavirus (HPV) genomes usually persist as episomal molecules in HPV associated preneoplastic lesions whereas they are frequently integrated into the host cell genome in HPV-related cancers cells. This suggests that malignant conversion of HPV-infected epithelia is linked to recombination of cellular and vi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast and cost-effective alternative to the conventional smear test. However, it is currently limited by significant inter-patient variation in the spectroscopic properties of the cervix. Characterisation of suitable in vit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22517
更新日期:2007-05-01 00:00:00
abstract::The human MUC1 gene expresses at least 2 type 1 membrane proteins: MUC1/REP, a polymorphic high m.w. MUC1 glycoprotein often highly expressed in breast cancer tissues and containing a variable number of tandem 20 amino acid repeat units, and the MUC1/Y protein, which lacks this repeat array and, therefore, is not poly...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970529)71:5<741::aid-ijc
更新日期:1997-05-29 00:00:00
abstract::Although early detection and systemic therapies have improved the diagnosis and clinical cure rate of breast cancer, breast cancer remains the most frequently occurring malignant cancer in women due to a lack of sufficiently effective treatments. Thus, to develop potential targeted therapies and thus benefit more pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32922
更新日期:2020-07-15 00:00:00
abstract::Modern life involves mistimed sleeping and eating patterns that in experimental studies are associated with adverse health effects. We assessed whether timing of meals is associated with breast and prostate cancer risk taking into account lifestyle and chronotype, a characteristic correlating with preference for morni...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31649
更新日期:2018-11-15 00:00:00
abstract::The aim of this study was to determine whether the skin-sensitizing agent 2,4-dinitrofluorobenzene (DNFB) would elicit the same morphological and biochemical events that are characteristic of 12-O-tetradecanoylphorbol-13-acetate (TPA). While single applications of 0.1% or 0.2% DNFB produced only mild epidermal hyperpl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600420
更新日期:1995-02-08 00:00:00
abstract::We aimed to identify risk factors for Kaposi's sarcoma (KS) among HIV-positive patients and behaviors associated with human Herpesvirus 8 (HHV-8) infection, as well as to assess KS incidence and mortality rates longitudinally. To fulfill the first objective, a European case-control study was designed in the early 1990...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.22281
更新日期:2007-03-01 00:00:00
abstract::The U.S. Preventive Services Task Force (USPSTF) recently updated their national lung screening guidelines and recommended low-dose computed tomography (LDCT) for lung cancer (LC) screening through age 80. However, the risk of overdiagnosis among older populations is a concern. Using four comparative models from the C...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30602
更新日期:2017-06-01 00:00:00
abstract::Individuals differ in their ability to repair DNA damage induced by carcinogens. Studies have shown that polymorphisms in DNA repair genes contribute to individual variation in DNA repair capacity and cancer risk. In a hospital-based case-control study, we tested the hypothesis that a C to T variant (Thr241Met) of DNA...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10413
更新日期:2002-06-20 00:00:00
abstract::Changes in the expression of a cell membrane antigen during hepatocarcinogenesis and in the developing liver were analyzed by HAM.4, a monoclonal antibody (MAb) against a membrane glycoprotein of normal rat hepatocyte. Of the precancerous lesions observed during hepatocarcinogenesis induced by diethylnitrosamine, 2-ac...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410418
更新日期:1988-04-15 00:00:00
abstract::Fine-needle aspiration cytology (FNAC) is currently used in evaluating the nature of breast nodules. In the present study we have examined whether monoclonal antibodies (MAbs) to breast-tumor-associated antigens (BTAA) can be employed in FNAC for the diagnosis of breast cancer. For this purpose we have used 2 murine M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370404
更新日期:1986-04-15 00:00:00
abstract::Inflammation, and more generally, the immune response are thought to influence the development of prostate cancer. To determine the components of the immune response that are potentially contributory, we prospectively evaluated the association of immune-mediated conditions, asthma and hayfever, with lethal prostate ca...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29463
更新日期:2015-08-15 00:00:00
abstract::Cancer blood vessels consist of two interacting types of cells: inner lining endothelial cells (ECs) and surrounding perivascular cells (pericytes, vascular smooth muscle cells or mural cells). PDGFRbeta(CD140b)+ progenitor perivascular cells (PPC) can differentiate into pericytes and regulate vessel stability and vas...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25816
更新日期:2011-09-15 00:00:00
abstract::Several mouse models studying the MLL fusion-induced leukemic transformation showed that a myeloproliferation stage precedes leukemia or occurred as the only phenotype of hematological disorder in mice. We established 6 MLL/AF10(OM-LZ)-immortalized cell lines by retrovirally transducing the fusion gene into bone marro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24867
更新日期:2010-04-01 00:00:00
abstract::A highly immunogenic variant of the murine L1210 leukemia cell (L1210/LN-1) for generation of tumor immunity has been obtained from culture of L1210 cells originally fused with human Lesch-Nyhan fibroblasts. In L1210/LN-1 cells, no human chromosomes were identified and chromosomes M1 and No. 1 carried by the parent L1...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320419
更新日期:1983-10-15 00:00:00
abstract::Evidence suggests that the majority of lung cancer patients have tumour-derived genetic alterations in circulating plasma DNA, and that this may be developed as a diagnostic tool. To this end, we have studied 60 individuals attending bronchoscopy clinic, with symptoms suspicious of lung cancer, for genetic alterations...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1058>3
更新日期:2001-02-01 00:00:00
abstract::Obesity is associated with an increased risk of fatal prostate cancer. We aimed to elucidate the importance and relevant timing of obesity and weight change for prostate cancer progression. We identified 5,158 men diagnosed with localized prostate cancer (clinical stage T1/T2) from 1986 to 2012 in the Health Professio...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30803
更新日期:2017-09-01 00:00:00
abstract::Although tumor-specific T lymphocytes recognize tumor-associated antigens (TAA) present on their cell surface via major histocompatibility complex (MHC) molecules, T cells require other activating signals. These are provided by costimulatory molecules, including B7-1 (CD80), B7-2 (CD86) and intercellular adhesive mole...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990827)82:5<714::aid-ijc
更新日期:1999-08-27 00:00:00
abstract::The ability to monitor breast cancer initiation and progression on the molecular level would provide an effective tool for early diagnosis and therapy. In the present study, we focused on the underglycosylated MUC-1 tumor antigen (uMUC-1), which is directly linked to tumor progression from pre-malignancy to advanced m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27872
更新日期:2013-04-15 00:00:00
abstract::Phenotypic and functional characteristics of tumor-infiltrating lymphocytes (TIL) obtained from human primary and metastatic liver tumors were studied. Lymphocytes isolated from 18 tumors and autologous (A) peripheral blood (6 cases) were phenotyped by 2-color flow cytometry and cloned in a limiting dilution system, w...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460521
更新日期:1990-11-15 00:00:00
abstract::A variety of analytical approaches have indicated that melanoma cell line UCLA-SO-M14 (M14) and breast carcinoma cell line MDA-MB-435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor an...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31067
更新日期:2018-02-01 00:00:00