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Quantification of mitochondrial DNA using real-time polymerase chain reaction in patients with premature ovarian failure.

Abstract：

OBJECTIVE:To quantify mitochondrial DNA using real-time PCR in women with premature ovarian failure (POF) and a control group. DESIGN:Prospective study. SETTING:Genome Research Center for Reproductive Medicine and Infertility, Korea Ministry of Health & Welfare. PATIENT(S):Thrity patients with POF and 30 control individuals. INTERVENTION(S):The mitochondrial DNA content was quantified using real-time PCR. The effectiveness of the assay was determined by relative quantification using the comparative threshold cycle (CT) method. MAIN OUTCOME MEASURE(S):Relative quantification of mitochondrial DNA content. RESULT(S):The mitochondrial DNA content was significantly lower in the POF group than in the control group (0.58 +/- 0.38 vs. 1.15 +/- 0.67; P < .01). In both groups, there was a significant positive correlation between the mitochondrial DNA/28S rRNA ratio and mitochondrial DNA CT (control group: r = 0.774; P < .001; POF group: r = 0.556; P = .001) and a significant negative correlation between the mitochondrial DNA/28S rRNA ratio and 28S rRNA CT (control group: r = -0.677; P < .001; POF group: r = -0.627; P = .001). CONCLUSION(S):This study has established the clinical feasibility of quantifying amounts of mitochondrial DNA, relative to an internal standard, using real-time PCR. Further studies are warranted to elucidate the roles of apoptosis and mitochondrial function in the pathogenesis of POF.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Cha KY,Lee SH,Chung HM,Baek KH,Cho SW,Kwack KB

doi

10.1016/j.fertnstert.2005.06.036

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1712-8

issue

6

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(05)03333-9

journal_volume

84

pub_type

临床试验,杂志文章

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