Abstract：
:Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS); therefore, studies have been carried out in order to better understand its damaging action in biological systems. In this work, calf thymus DNA, triphosphate nucleotides and isolated bases were incubated with SnCl2 and the results were analyzed through UV spectrophotometry. The presence of stannous ions altered the absorption spectra of all three isolates. The amount of stannous ions associated to DNA was measured by atomic absorption spectrophotometry. Data showed that more than 40% of the initial SnCl2 concentration was present in the samples. Our results are in accordance with the damaging potential of this salt and present evidence that stannous ions can complex with DNA, inducing ROS in its vicinity, which may be responsible for the observed lesions.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
de Mattos JC,Lage C,Dantas FJ,Moraes MO,Nunes AP,Bezerra RJ,Faria MV,Leitão AC,Caldeira-de-Araujo Adoi
10.1007/s11010-005-8908-6keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
173-9issue
1-2eissn
0300-8177issn
1573-4919journal_volume
280pub_type
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