Tubulin nitration in human gliomas.

Abstract:

:Immunohistochemical and biochemical investigations showed that significant protein nitration occurs in human gliomas, especially in grade IV glioblastomas at the level of astrocytes and oligodendrocytes and neurones. Enhanced alpha-tubulin immunoreactivity was co-present in the same elements in the glioblastomas. Proteomic methodologies were employed to identify a nitrated protein band at 55 kDa as alpha-tubulin. Peptide mass fingerprinting procedures demonstrated that tubulin is nitrated at Tyr224 in grade IV tumour samples but is unmodified in grade I samples and in non-cancerous brain tissue. These results provide the first characterisation of endogenously nitrated tubulin from human tumour samples.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Fiore G,Di Cristo C,Monti G,Amoresano A,Columbano L,Pucci P,Cioffi FA,Di Cosmo A,Palumbo A,d'Ischia M

doi

10.1016/j.neulet.2005.10.011

keywords:

subject

Has Abstract

pub_date

2006-02-06 00:00:00

pages

57-62

issue

1

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(05)01161-4

journal_volume

394

pub_type

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