Abstract：
:The CDC4 gene of Saccharomyces cerevisiae encodes an essential function that is required for G1-S and G2-M transitions during mitosis and at various stages during meiosis. We have isolated a functional homologue of CDC4 (CaCDC4) from the pathogenic yeast Candida albicans by complementing the S. cerevisiae cdc4-3 mutation with CaCDC4 expressed from its own promoter on a single-copy vector. The predicted product of CaCDC4 has 37% overall identity to the S. cerevisiae Cdc4 protein, although this identity is biased towards the C-terminal region of the two proteins which contains eight copies of the degenerate WD-40 motif, an element found in proteins that regulate diverse biological processes and an F-box domain proximal to the first iteration of the WD-40 motif. Both the F-box domain and WD-40 motifs appear necessary for the mitotic functions of Cdc4 in both yeasts. In contrast to its conserved role in mitosis, C. albicans CDC4 is unable to rescue the meiotic deficiency in a S. cerevisiae cdc4 homozygous diploid under restrictive conditions, even when expressed from an efficient S. cerevisiae promoter. In opposition to S. cerevisiae CDC4 being essential, C. albicans CDC4 appears to be nonessential and in its absence is critical for filamentous growth in C. albicans.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Shieh JC,White A,Cheng YC,Rosamond Jdoi
10.1007/s11373-005-9027-9keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
913-24issue
6eissn
1021-7770issn
1423-0127journal_volume
12pub_type
杂志文章abstract：BACKGROUND:To mimic systemic inflammation in humans, different animal models have been developed. Since these models are still discussed controversially, we aimed to comparatively evaluate the most widely used models with respect to the systemic effects, the influence on organ functions and to the underlying pathophysi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0370-8
更新日期：2017-08-24 00:00:00
abstract：BACKGROUND:RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally. Delivering sequences of RNAi in vivo remains a problem. The aim of this study was to use JC virus (JCV) virus-like particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene of IL-10. METHODS:J...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-51
更新日期：2010-06-24 00:00:00
abstract：BACKGROUND:NKG2D, an activating immunoreceptor, is primarily restricted to NK cells and CD8(+) T cells. The existence of an atypical cytotoxic CD4(+)NKG2D(+) T cell population has also been found in patients with autoimmune dysfunctions. Nonetheless, contradictory evidence has categorized this population with a regulat...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0190-7
更新日期：2015-10-20 00:00:00
abstract：BACKGROUND:Increased levels of blood plasma urea were used as phenotypic parameter for establishing novel mouse models for kidney diseases on the genetic background of C3H inbred mice in the phenotype-driven Munich ENU mouse mutagenesis project. The phenotypically dominant mutant line HST014 was established and further...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0365-5
更新日期：2017-08-17 00:00:00
abstract：:Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000079667
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:Adipogenesis is a complex process that involves many genes/proteins at different stages of differentiation. In order to identify genes critical for adipogenesis, we took a novel approach based on phenotype change of individual cell, to search for genes with regulatory roles in adipogenesis genome-wide in 3T3...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-92
更新日期：2009-10-13 00:00:00
abstract：BACKGROUND:Detection of cholangiocarcinoma (CCA) remains a diagnostic challenge. We established diagnostic peptide biomarkers in bile and urine based on capillary electrophoresis coupled to mass spectrometry (CE-MS) to detect both local and systemic changes during CCA progression. In a prospective cohort study we recen...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0599-5
更新日期：2020-01-03 00:00:00
abstract：:We previously reported that transgenic ablation of gonadotrophs results in impaired development of cells immunostainable for prolactin (PRL) but not of cells immunostainable for growth hormone (GH) or pro-opiomelanocortin (POMC) in pituitary of newborn mice. The question remained whether this reduction in PRL protein ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000073968
更新日期：2003-11-01 00:00:00
abstract：:Microfluidics has become an important tool in diagnosing many diseases, including neurological and genetic disorders. Alzheimer's disease (AD) is a neurodegenerative disease that irreversibly and progressively destroys memory, language ability, and thinking skills. Commonly, detection of AD is expensive and complex. F...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/1423-0127-18-33
更新日期：2011-05-28 00:00:00
abstract：:Peptidoglycan-activated gene expression is mediated through various transcription factors including CCAAT/enhancer-binding protein delta (C/EBPdelta). The purpose of the present study is to elucidate the mechanism of PGN-activated C/EBPdelta gene. PGN stimulated C/EBPdelta protein and mRNA expression in mouse macropha...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9146-6
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:To develop an individually-tailored dynamic risk assessment model following a multistep, multifactorial process of the Correa's gastric cancer model. METHODS:First, we estimated the state-to-state transition rates following Correa's five-step carcinogenic model and assessed the effect of risk factors, inclu...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0485-6
更新日期：2018-11-19 00:00:00
abstract：BACKGROUND:Next-generation sequencing (NGS) is a powerful and high-throughput method for the detection of viral mutations. This article provides a brief overview about optimization of NGS analysis for hepatocellular carcinoma (HCC)-associated hepatitis B virus (HBV) mutations, and hepatocarcinogenesis of relevant mutat...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0442-4
更新日期：2018-06-02 00:00:00
abstract：:Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/s11373-005-9063-5
更新日期：2006-03-01 00:00:00
abstract：:Exposure of cells and organs to heat shock is associated with numerous changes in various cellular metabolic parameters and overexpression of proteins collectively known as heat shock proteins (HSP). In this communication we review the cell-signaling events that are altered in response to heat shock as they relate to ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02258043
更新日期：1996-11-01 00:00:00
abstract：:The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a sign...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253367
更新日期：2000-11-01 00:00:00
abstract：:Adenosine was shown to inhibit norepinephrine (NE) release from sympathetic nerve endings. The purpose of this study was to examine whether endogenous adenosine restrains NE and epinephrine release from the adrenal medulla. The effects of an adenosine receptor antagonist, 1,3-dipropyl-8-(p-sulfophenyl) xanthine (DPSPX...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255947
更新日期：2001-09-01 00:00:00
abstract：BACKGROUND:Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. METHODS:Two normal, LB and HSC93, and two transformed, Ju...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-63
更新日期：2010-07-28 00:00:00
abstract：:We studied 42 individuals, including 8 patients with either complete or partial syndrome of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS), 8 patients with either complete or partial syndrome of myoclonic epilepsy with ragged-red fibers (MERRF) and 26 maternal family members w...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000064725
更新日期：2002-01-01 00:00:00
abstract：:To determine the involvement of the globus pallidus in mediating epilepsy, the effects of microinjection of a GABA uptake blocker (tiagabine), a benzodiazepine agonist (zolpidem) and a GABA-B receptor agonist (baclofen) on pentylenetetrazol (PTZ)-induced tonic seizure were examined in adult rats. Administration of PTZ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256094
更新日期：2004-07-01 00:00:00
abstract：:Based on the tertiary structure of the ribosome-inactivating protein alpha-sarcin, domains that are responsible for hydrolyzing ribosomes and naked RNA have been dissected. In this study, we found that the head-to-tail interaction between the first amino beta-strand and the last carboxyl beta-strand is not involved in...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255817
更新日期：2000-09-01 00:00:00
abstract：:The ARFP/F protein is synthesized from the +1 reading frame of the hepatitis C virus (HCV) core protein gene. The function of this protein remains unknown. To study the function of the HCV ARFP/F protein, we have conducted the yeast two-hybrid screening experiment to identify cellular proteins that may interact with t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9248-9
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Loss of ovarian function, as in menopause or after ovariectomy (OVX), is closely associated with obesity and white adipose tissue (WAT) inflammation. Estrogen replacement protects against postmenopausal obesity but increases the risks of carcinogenesis. In the present study, we investigated the effects of lo...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0556-3
更新日期：2019-08-31 00:00:00
abstract：:Our objective was to determine the role that bone marrow-derived stromal cells have on human hematopoiesis in HIV infection. In particular, we dissected the heterogeneous bone marrow microenvironment to study the effect HIV expression might have on the cell population capable of producing the cytokines which will supp...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255220
更新日期：1995-10-01 00:00:00
abstract：BACKGROUND:Chemotherapy and targeted therapies have made important strides in cancer treatment yet they often fail and new therapies are still needed. Here, we employed a phenotypic screen to identify and analyze the mechanism of action of novel small molecules that interfere with critical pathways involved in tumor ce...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0408-6
更新日期：2018-02-08 00:00:00
abstract：BACKGROUND:The enzyme-prodrug system is considered a promising tool for tumor treatment when conjugated with a targeting molecule. The asparagine-glycine-arginine (NGR) motif is a developing and interesting targeting peptide that could specifically bind to aminopeptidase N (APN), which is an NGR receptor expressed on t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0227-6
更新日期：2016-01-22 00:00:00
abstract：BACKGROUND:Oxidative stress is a major factor in retinal pigment epithelium (RPE) cells injury that contributes to age-related macular degeneration (AMD). NaIO3 is an oxidative toxic agent and its selective RPE cell damage makes it as a reproducible model of AMD. Although NaIO3 is an oxidative stress inducer, the roles...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0531-z
更新日期：2019-05-22 00:00:00
abstract：BACKGROUND:Zerumbone, a sesquiterpene compound isolated from subtropical ginger, Zingiber zerumbet Smith, has been documented to exert antitumoral and anti- inflammatory activities. In this study, we demonstrate that zerumbone induces apoptosis in human glioblastoma multiforme (GBM8401) cells and investigate the apopto...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-86
更新日期：2012-10-05 00:00:00
abstract：:Dengue virus infection presents a wide spectrum of manifestations including asymptomatic condition, dengue fever (DF), or severe forms, such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) in affected individuals. The early prediction of severe dengue in patients without any warning signs who may lat...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-015-0191-6
更新日期：2015-10-14 00:00:00
abstract：:A considerable number of animal studies on the effects of dopaminergic agents on alcohol intake behavior have been performed. Acute alcohol administration in rats induces dopamine release in the caudate nucleus and in the nucleus accumbens, an effect related among others to reinforcement. It has been repeatedly sugges...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02255975
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:The probability of local tumor control after radiotherapy (RT) remains still miserably poor in pediatric rhabdomyosarcoma (RMS). Thus, understanding the molecular mechanisms responsible of tumor relapse is essential to identify personalized RT-based strategies. Contrary to what has been done so far, a correc...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00683-6
更新日期：2020-08-27 00:00:00
