Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen.

abstract：:We have previously described how T and natural killer (NK) lineage commitment proceeds from common T/NK progenitors (p-T/NK) in the murine fetal thymus (FT), with the use of a clonal assay system capable of discriminating p-T/NK from unipotent T or NK lineage-committed progenitors (p-T and p-NK, respectively). The mol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikawa T,Fujimoto S,Kawamoto H,Katsura Y,Yokota Y

更新日期：2001-04-24 00:00:00

abstract：:The production of hybridomas between immunologically activated T cells and malignant T-cell lines offers a potentially unlimited source of soluble T-cell-derived products. Recently, human T-T hybrids have been described; however, their use has been hampered by slow growth and chromosomal instability due at least in pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foung SK,Sasaki DT,Grumet FC,Engleman EG

更新日期：1982-12-01 00:00:00

abstract：:Bone remodeling requires osteoclasts to generate and maintain an acidified resorption compartment between the apical membrane and the bone surface to solubilize hydroxyapatite crystals within the bone matrix. This acidification process requires (i) apical proton secretion by a vacuolar H(+)-ATPase, (ii) actin cytoskel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coury F,Zenger S,Stewart AK,Stephens S,Neff L,Tsang K,Shull GE,Alper SL,Baron R,Aliprantis AO

更新日期：2013-02-05 00:00:00

abstract：:The CD4 receptor contributes to T-cell activation by coligating major histocompatibility complex class II on antigen presenting cells with the T-cell receptor (TCR)/CD3 complex, and triggering a cascade of signaling events including tyrosine phosphorylation of intracellular proteins. Paradoxically, CD3 cross-linking p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Desbarats J,Freed JH,Campbell PA,Newell MK

更新日期：1996-10-01 00:00:00

abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) in the endoplasmic reticulum mediates calcium signaling that impinges on intracellular processes. IP3Rs are allosteric proteins comprising four subunits that form an ion channel activated by binding of IP3 at a distance. Defective allostery in IP3R is considered crucial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamada K,Terauchi A,Nakamura K,Higo T,Nukina N,Matsumoto N,Hisatsune C,Nakamura T,Mikoshiba K

更新日期：2014-09-23 00:00:00

abstract：:McKay et al. [(1996) Science 273, 924-930] suggested that carbonate globules in the meteorite ALH84001 contained the fossil remains of Martian microbes. We have characterized a subpopulation of magnetite (Fe(3)O(4)) crystals present in abundance within the Fe-rich rims of these carbonate globules. We find these Martia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Keprta KL,Clemett SJ,Bazylinski DA,Kirschvink JL,McKay DS,Wentworth SJ,Vali H,Gibson EK Jr,McKay MF,Romanek CS

更新日期：2001-02-27 00:00:00

abstract：:In Saccharomyces cerevisiae, utilization of galactose requires four inducible enzyme activities. Three of these activities (galactose-1-phosphate uridyl transferase, EC 2.7.7.10; uridine diphosphogalactose 4-epimerase, EC 5.1.3.2; and galactokinase, EC 2.7.1.6) are specified by three tightly linked genes (GAL7, GAL10,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hopper JE,Broach JR,Rowe LB

更新日期：1978-06-01 00:00:00

abstract：:Thebaine, an intermediate of morphine biosynthesis in the poppy plant, Papaver somniferum, was transformed to oripavine, codeine, and morphine by rat liver, kidney, and brain microsomes in the presence of an NADPH-generating system. The formation of morphine, codeine, and oripavine was identified by a specific RIA, HP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kodaira H,Spector S

更新日期：1988-02-01 00:00:00

abstract：:Antigen-induced activation of T cells can be specifically inhibited by antigen analogs that have been termed T-cell receptor peptide antagonists. These antagonists appear to act by inducing the formation of nonstimulatory or partially stimulatory complexes between T-cell receptors and the major histocompatibility comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Page DM,Alexander J,Snoke K,Appella E,Sette A,Hedrick SM,Grey HM

更新日期：1994-04-26 00:00:00

abstract：:In the cell, proteins are synthesized from N to C terminus and begin to fold during translation. Cotranslational folding mechanisms are therefore linked to elongation rate, which varies as a function of synonymous codon usage. However, synonymous codon substitutions can affect many distinct cellular processes, which h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh IM,Bowman MA,Soto Santarriaga IF,Rodriguez A,Clark PL

更新日期：2020-02-18 00:00:00

abstract：:Hyperpolarization of substrates for magnetic resonance spectroscopy (MRS) and imaging (MRI) by dissolution dynamic nuclear polarization (D-DNP) usually involves saturating the ESR transitions of polarizing agents (PAs; e.g., persistent radicals embedded in frozen glassy matrices). This approach has shown enormous pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gajan D,Bornet A,Vuichoud B,Milani J,Melzi R,van Kalkeren HA,Veyre L,Thieuleux C,Conley MP,Grüning WR,Schwarzwälder M,Lesage A,Copéret C,Bodenhausen G,Emsley L,Jannin S

更新日期：2014-10-14 00:00:00

abstract：:Asthma resistance to glucocorticoid treatment is a major health problem with unclear etiology. Glucocorticoids inhibit adrenal androgen production. However, androgens have potential benefits in asthma. HSD3B1 encodes for 3β-hydroxysteroid dehydrogenase-1 (3β-HSD1), which catalyzes peripheral conversion from adrenal de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zein J,Gaston B,Bazeley P,DeBoer MD,Igo RP Jr,Bleecker ER,Meyers D,Comhair S,Marozkina NV,Cotton C,Patel M,Alyamani M,Xu W,Busse WW,Calhoun WJ,Ortega V,Hawkins GA,Castro M,Chung KF,Fahy JV,Fitzpatrick AM,Israel

更新日期：2020-01-28 00:00:00

abstract：:Myocardial ischemic disease is the major cause of death worldwide. After myocardial infarction, reperfusion of infracted heart has been an important objective of strategies to improve outcomes. However, cardiac ischemia/reperfusion (I/R) is characterized by inflammation, arrhythmias, cardiomyocyte damage, and, at the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fauconnier J,Meli AC,Thireau J,Roberge S,Shan J,Sassi Y,Reiken SR,Rauzier JM,Marchand A,Chauvier D,Cassan C,Crozier C,Bideaux P,Lompré AM,Jacotot E,Marks AR,Lacampagne A

更新日期：2011-08-09 00:00:00

abstract：:Livers of a natural population of winter flounder from a contaminated site in Boston Harbor were examined for the presence of oncogenes by transfection of DNA into NIH 3T3 mouse fibroblasts. Tissues analyzed contained histopathologic lesions including abnormal vacuolation, biliary proliferation, and, in many cases, he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McMahon G,Huber LJ,Moore MJ,Stegeman JJ,Wogan GN

更新日期：1990-01-01 00:00:00

abstract：:Generation of nanomechanical cantilever motion from biomolecular interactions can have wide applications, ranging from high-throughput biomolecular detection to bioactuation. Although it has been suggested that such motion is caused by changes in surface stress of a cantilever beam, the origin of the surface-stress ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu G,Ji H,Hansen K,Thundat T,Datar R,Cote R,Hagan MF,Chakraborty AK,Majumdar A

更新日期：2001-02-13 00:00:00

abstract：:The highly aggressive character of melanoma makes it an excellent model for probing the mechanisms underlying metastasis, which remains one of the most difficult challenges in treating cancer. We find that miR-182, member of a miRNA cluster in a chromosomal locus (7q31-34) frequently amplified in melanoma, is commonly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segura MF,Hanniford D,Menendez S,Reavie L,Zou X,Alvarez-Diaz S,Zakrzewski J,Blochin E,Rose A,Bogunovic D,Polsky D,Wei J,Lee P,Belitskaya-Levy I,Bhardwaj N,Osman I,Hernando E

更新日期：2009-02-10 00:00:00

abstract：:In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis of these isoforms, we have sequenced a 5.8-kilobase region of the Drosophila genome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Kalm L,Weaver J,DeMarco J,MacIntyre RJ,Sullivan DT

更新日期：1989-07-01 00:00:00

abstract：:Microinjection of progesterone dissolved in paraffin oil induces the reinitiation of meiotic maturation in the Xenopus oocyte; 50% maturation is obtained when 50 nl of a 50 microM solution is microinjected into the oocyte. The kinetics of the response to microinjected progesterone are similar to the kinetics of respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tso J,Thibier C,Mulner O,Ozon R

更新日期：1982-09-01 00:00:00

abstract：:The structures of the major folding intermediate, the transition state for folding, and the folded state of barnase have been previously characterized. We now add a further step toward a complete picture of the folding of barnase by reporting the backbone 15N, 13C, and 1H NMR assignments for barnase unfolded at pH 1.8...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arcus VL,Vuilleumier S,Freund SM,Bycroft M,Fersht AR

更新日期：1994-09-27 00:00:00

abstract：:Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that rob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwasaki H,Nishiwaki T,Kitayama Y,Nakajima M,Kondo T

更新日期：2002-11-26 00:00:00

abstract：:Mucosa-associated lymphoid tissue (MALT) lymphoma is the most common extranodal lymphoid cell neoplasia; it frequently follows chronic bacteria-induced inflammation in various tissues. MALT lymphomas are characterized genetically by the t(11;18)(q21;q21) translocation, which yields chimeric transcripts encoding struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stoffel A,Chaurushiya M,Singh B,Levine AJ

更新日期：2004-06-15 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHH) is a key enzyme in transmethylation reactions that use S-adenosylmethionine as the methyl donor. Because of the importance of SAHH in a number of S-adenosylmethionine-dependent transmethylation reactions, particularly the 5' capping of mRNA during viral replication, SAHH has bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuta C,Tanaka H,Uehara K,Kuwata S,Koiwai A,Noma M

更新日期：1995-06-20 00:00:00

abstract：:We demonstrate that the receptor binding moiety of Escherichia coli heat-labile enterotoxin (EtxB) can completely prevent autoimmune disease in a murine model of arthritis. Injection of male DBA/1 mice at the base of the tail with type II collagen in the presence of complete Freund's adjuvant normally leads to arthrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams NA,Stasiuk LM,Nashar TO,Richards CM,Lang AK,Day MJ,Hirst TR

更新日期：1997-05-13 00:00:00

abstract：:Oscillations in intracellular free calcium concentration ([Ca(2+)](i)) occur spontaneously in immature neurons of the developing cerebral cortex. Here, we show that developing murine cortical neurons exhibit calcium oscillations in response to direct activation of the mGluR5 subtype of the group I metabotropic glutama...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flint AC,Dammerman RS,Kriegstein AR

更新日期：1999-10-12 00:00:00

abstract：:Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the CNS that causes disability in young adults as a result of the irreversible accumulation of neurological deficits. Although there are potent disease-modifying agents for its initial relapsing-remitting phase, these therapies show limited...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothhammer V,Kenison JE,Tjon E,Takenaka MC,de Lima KA,Borucki DM,Chao CC,Wilz A,Blain M,Healy L,Antel J,Quintana FJ

更新日期：2017-02-21 00:00:00

abstract：:Mycobacterium abscessus (Mabs) is a rapidly growing Mycobacterium and an emerging pathogen in humans. Transitioning from a smooth (S) high-glycopeptidolipid (GPL) producer to a rough (R) low-GPL producer is associated with increased virulence in zebrafish, which involves the formation of massive serpentine cords, absc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halloum I,Carrère-Kremer S,Blaise M,Viljoen A,Bernut A,Le Moigne V,Vilchèze C,Guérardel Y,Lutfalla G,Herrmann JL,Jacobs WR Jr,Kremer L

更新日期：2016-07-19 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) viral DNA synthesis in quiescent and activated peripheral blood lymphocytes (PBLs) was studied. Incomplete viral DNA (previously demonstrated to be associated with HIV-1 virions) is carried by HIV-1 virions into quiescent and activated PBLs, contributing to the formation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao WY,Cara A,Gallo RC,Lori F

更新日期：1993-10-01 00:00:00

abstract：:Influenza hemagglutinin (HA) glycoprotein is the primary surface antigen targeted by the host immune response and a focus for development of novel vaccines, broadly neutralizing antibodies (bnAbs), and therapeutics. HA enables viral entry into host cells via receptor binding and membrane fusion and is a validated targ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao Y,Kadam RU,Lee CD,Woehl JL,Wu NC,Zhu X,Kitamura S,Wilson IA,Wolan DW

更新日期：2020-08-04 00:00:00

abstract：:The bacterial pathogen Helicobacter pylori chronically infects the human gastric mucosa and is the leading risk factor for the development of gastric cancer. The molecular mechanisms of H. pylori-associated gastric carcinogenesis remain ill defined. In this study, we examined the possibility that H. pylori directly co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toller IM,Neelsen KJ,Steger M,Hartung ML,Hottiger MO,Stucki M,Kalali B,Gerhard M,Sartori AA,Lopes M,Müller A

更新日期：2011-09-06 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease of motor neurons. The inherited form of the disease, familial ALS, represents 5-10% of the total cases, and the best documented of these are due to lesions in SOD1, the gene encoding copper-zinc superoxide dismutase (CuZnSOD). The mechanism by w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valentine JS,Hart PJ

更新日期：2003-04-01 00:00:00