Synonymous codon substitutions perturb cotranslational protein folding in vivo and impair cell fitness.

abstract：:Lon (or La) is a soluble, homooligomeric ATP-dependent protease. Mass determination and cryoelectron microscopy of pure mitochondrial Lon from Saccharomyces cerevisiae identify Lon as a flexible ring-shaped heptamer. In the presence of ATP or 5'-adenylylimidodiphosphate, most of the rings are symmetric and resemble ot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stahlberg H,Kutejová E,Suda K,Wolpensinger B,Lustig A,Schatz G,Engel A,Suzuki CK

更新日期：1999-06-08 00:00:00

abstract：:A photosensitizer conjugate, chlorin e6 (Ce6) covalently bound to 1-micron-diameter polystyrene microspheres, has been investigated in the photodynamic destruction of MGH-U1 human bladder carcinoma cells in vitro. The microspheres were taken up avidly by the carcinoma cells; confocal laser scanning fluorescence micros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachor R,Shea CR,Gillies R,Hasan T

更新日期：1991-02-15 00:00:00

abstract：:Cocaine addiction and overdose have long defied specific treatment. To provide a new approach, the high-activity catalytic antibody mAb 15A10 was elicited using a transition-state analog for the hydrolysis of cocaine to nontoxic, nonaddictive products. In a model of cocaine overdose, mAb 15A10 protected rats from coca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mets B,Winger G,Cabrera C,Seo S,Jamdar S,Yang G,Zhao K,Briscoe RJ,Almonte R,Woods JH,Landry DW

更新日期：1998-08-18 00:00:00

abstract：:Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% iden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCoy JG,Bailey LJ,Bitto E,Bingman CA,Aceti DJ,Fox BG,Phillips GN Jr

更新日期：2006-02-28 00:00:00

abstract：:Autism spectrum disorders (ASDs) comprise a highly heritable, multifarious group of neurodevelopmental disorders, which are characterized by repetitive behaviors and impairments in social interactions. Point mutations have been identified in X-linked Neuroligin (NLGN) 3 and 4X genes in patients with ASDs and all of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bemben MA,Nguyen QA,Wang T,Li Y,Nicoll RA,Roche KW

更新日期：2015-02-24 00:00:00

abstract：:The drug resistance factor RP4, originally isolated in Pseudomonas, was transferred to Rhizobium meliloti. In that strain, RP4 promotes conjugational transfer of chromosomal markers to form haploid recombinants. This mating system has been used to construct a linkage map of R. meliloti. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meade HM,Signer ER

更新日期：1977-05-01 00:00:00

abstract：:Understanding the molecular determinants of specificity in protein-protein interaction is an outstanding challenge of postgenome biology. The availability of large protein databases generated from sequences of hundreds of bacterial genomes enables various statistical approaches to this problem. In this context covaria...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigt M,White RA,Szurmant H,Hoch JA,Hwa T

更新日期：2009-01-06 00:00:00

abstract：:The binding of invariant chain to major histocompatibility complex (MHC) proteins is an important step in processing of MHC class II proteins and in antigen presentation. The question of how invariant chain can bind to all MHC class II proteins is central to understanding these processes. We have employed molecular mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee C,McConnell HM

更新日期：1995-08-29 00:00:00

abstract：:Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Here, an approximately 20-kDa caveolin-related protein, caveolin-2, was ide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scherer PE,Okamoto T,Chun M,Nishimoto I,Lodish HF,Lisanti MP

更新日期：1996-01-09 00:00:00

abstract：:The protooncogene src encodes two proteins, designated pp60c-src+ and pp60c-src.pp60c-src+ is expressed only in neurons, whereas pp60c-src is expressed in neuronal and nonneuronal cells. pp60c-src+ differs from pp60c-src in that it contains an insert of 6 amino acids. To study the evolutionary conservation of the 6-am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang XM,Martinez R,Le Beau J,Wiestler O,Walter G

更新日期：1989-06-01 00:00:00

abstract：:Viruses modulate biochemical cellular pathways to permit infection. A recently described mechanism mediates selective protein interactions between acidic domain readers and unacetylated, lysine-rich regions, opposite of bromodomain function. Kaposi´s sarcoma (KS)-associated herpesvirus (KSHV) is tightly linked with KS...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juillard F,de Miranda MP,Li S,Franco A,Seixas AF,Liu B,Álvarez ÁL,Tan M,Szymula A,Kaye KM,Simas JP

更新日期：2020-09-08 00:00:00

abstract：:High-grade neuroendocrine lung malignancies (large-cell neuroendocrine cell carcinoma, LCNEC, and small-cell lung carcinoma, SCLC) are among the most deadly lung cancer conditions with no optimal clinical management. The biological relationships between SCLC and LCNEC are still largely unknown and a current matter of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lázaro S,Pérez-Crespo M,Lorz C,Bernardini A,Oteo M,Enguita AB,Romero E,Hernández P,Tomás L,Morcillo MÁ,Paramio JM,Santos M

更新日期：2019-10-29 00:00:00

abstract：:We have constructed and cloned, in bacteria, recombinant plasmids containing DNA complementary to mRNA coding for a pancreatic pre-prosomatostatin, a product of the cell-free translation of pancreatic islet mRNAs shown previously by immunoprecipitation to be a precursor of somatostatin. A clone containing a nearly ful...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodman RH,Jacobs JW,Chin WW,Lund PK,Dee PC,Habener JF

更新日期：1980-10-01 00:00:00

abstract：:Imprinted gene expression associated with Prader-Willi syndrome (PWS) and Angelman syndrome (AS) is controlled by two imprinting centers (ICs), the PWS-IC and the AS-IC. The PWS-IC operates in cis to activate transcription of genes that are expressed exclusively from the paternal allele. We have created a conditional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DuBose AJ,Smith EY,Johnstone KA,Resnick JL

更新日期：2012-02-28 00:00:00

abstract：:Isolated yeast mitochondria, which synthesize identifiable polypeptides identical to those made in vivo, have been used in an invitro system to study cytoplasmic control of mitochondrial protein synthesis. It has been found that protein synthesis in isolated mitochondria is dependent on an endogenous pool of cytoplasm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poyton RO,Kavanagh J

更新日期：1976-11-01 00:00:00

abstract：:Leukemia inhibitory factor (LIF) receptor is a cell surface receptor that mediates the actions of LIF and other IL-6 type cytokines through the formation of high-affinity signaling complexes with gp130. Here we present the crystal structure of a complex of mouse LIF receptor with human LIF at 4.0 A resolution. The str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huyton T,Zhang JG,Luo CS,Lou MZ,Hilton DJ,Nicola NA,Garrett TP

更新日期：2007-07-31 00:00:00

abstract：:A typical molecular ion mass spectrum consists of a sum of signals from species of various possible isotopic compositions. Only the monoisotopic peak (e.g., all carbons are 12C; all nitrogens are 14N, etc.) has a unique elemental composition. Every other isotope peak at approximately integer multiples of approximately...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi SD,Hendrickson CL,Marshall AG

更新日期：1998-09-29 00:00:00

abstract：:In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maita N,Okada K,Hatakeyama K,Hakoshima T

更新日期：2002-02-05 00:00:00

abstract：:We perform a generalized-ensemble simulation of a small peptide taking the interactions among all atoms into account. From this simulation we obtain thermodynamic quantities over a wide range of temperatures. In particular, we show that the folding of a small peptide is a multistage process associated with two charact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansmann UH,Masuya M,Okamoto Y

更新日期：1997-09-30 00:00:00

abstract：:Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brabec RK,Peters BP,Bernstein IA,Gray RH,Goldstein IJ

更新日期：1980-01-01 00:00:00

abstract：:Most studies on the ability of insect populations to transmit pathogens consider only constant temperatures and do not account for realistic daily temperature fluctuations that can impact vector-pathogen interactions. Here, we show that diurnal temperature range (DTR) affects two important parameters underlying dengue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lambrechts L,Paaijmans KP,Fansiri T,Carrington LB,Kramer LD,Thomas MB,Scott TW

更新日期：2011-05-03 00:00:00

abstract：:Pure cultures of human breast epithelial cells and of fibroblastic cells in early passage provided the opportunity to ask whether either cell type had the capability for metabolizing chemical carcinogens and, if so, was the fate of the metabolic products compatible with chemical carcinogens being a factor in the initi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stampfer MR,Bartholomew JC,Smith HS,Bartley JC

更新日期：1981-10-01 00:00:00

abstract：:This communication describes the preparation of antibodies to human interleukin 2 (IL-2), using as immunogens synthetic peptides derived from the predicted amino acid sequence of IL-2. Rabbits and mice were immunized with protein carrier conjugates of eight chemically synthesized IL-2-derived peptides, each consisting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Altman A,Cardenas JM,Houghten RA,Dixon FJ,Theofilopoulos AN

更新日期：1984-04-01 00:00:00

abstract：:Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deatly AM,Spivack JG,Lavi E,Fraser NW

更新日期：1987-05-01 00:00:00

abstract：:Large quantities of mineral dust particles are frequently ejected into the atmosphere through the action of wind. The surface of dust particles acts as a sink for many gases, such as sulfur dioxide. It is well known that under most conditions, sulfur dioxide reacts on dust particle surfaces, leading to the production ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dupart Y,King SM,Nekat B,Nowak A,Wiedensohler A,Herrmann H,David G,Thomas B,Miffre A,Rairoux P,D'Anna B,George C

更新日期：2012-12-18 00:00:00

abstract：:Positively acting, rate-limiting regulatory factors that influence tissue-specific expression of the human cardiac alpha-actin gene in a mouse muscle cell line are shown by in vivo competition and gel mobility-shift assays to bind to upstream regions of its promoter but to neither vector DNA nor a beta-globin promoter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miwa T,Boxer LM,Kedes L

更新日期：1987-10-01 00:00:00

abstract：:How likely is it that someone would approve of using a nuclear weapon to kill millions of enemy civilians in the hope of ending a ground war that threatens thousands of American troops? Ask them how they feel about prosecuting immigrants, banning abortion, supporting the death penalty, and protecting gun rights and yo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slovic P,Mertz CK,Markowitz DM,Quist A,Västfjäll D

更新日期：2020-08-25 00:00:00

abstract：:Recent evidence indicates a specific role for vascular endothelial growth factor a (Vegfa) during artery development in both zebrafish and mouse embryos, whereas less is known about signals that govern vein formation. In zebrafish, loss of vegfa blocks segmental artery formation and reduces artery-specific gene expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Covassin LD,Villefranc JA,Kacergis MC,Weinstein BM,Lawson ND

更新日期：2006-04-25 00:00:00

abstract：:Eukaryotic translation initiation factor eIF-4E plays a central role in the recognition of the 7-methylguanosine-containing cap structure of mRNA and the formation of initiation complexes during protein synthesis. eIF-4E exists in both phosphorylated and nonphosphorylated forms, and the primary site of phosphorylation...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minich WB,Balasta ML,Goss DJ,Rhoads RE

更新日期：1994-08-02 00:00:00

abstract：:We report here the identification of a pollen-specific gene from Zea mays that contains multiple Ser-(Pro)n repeats, the motif found in the cell wall-associated extensins. Sequence analysis reveals that the encoded protein has a putative globular domain at the N terminus and an extensin-like domain at the C terminus. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubinstein AL,Broadwater AH,Lowrey KB,Bedinger PA

更新日期：1995-04-11 00:00:00