Evolutionary expression of the neuronal form of the src protein in the brain.

abstract：:By using a systematic genetic approach, the resonances in the 19F NMR spectrum of 3-fluorotyrosine-substituted lac repressor protein have been assigned. The NMR data indicate that each monomer of the repressor consists of two distinct and independent domains. One domain, the NH2-terminal sixth of the primary sequence,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarema MA,Lu P,Miller JH

更新日期：1981-05-01 00:00:00

abstract：:Prader-Willi syndrome (PWS) and Angelman syndrome (AS) result from the loss of function of imprinted genes in human chromosome 15q11-q13. The central part of mouse chromosome 7 is homologous to human 15q11-q13, with conservation of both gene order and imprinted features. We report here the characterization of a transg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gabriel JM,Merchant M,Ohta T,Ji Y,Caldwell RG,Ramsey MJ,Tucker JD,Longnecker R,Nicholls RD

更新日期：1999-08-03 00:00:00

abstract：:Transcription of T7 DNA by T7 RNA polymerase in vitro gives rise to six major size classes of RNAs comprising seven major T7 RNA species. These RNAs are all read from the r-strand of T7 DNA and are not derived from the early (leftmost on the conventional genetic map) region of the molecule. When artifically shortened ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golomb M,Chamberlin M

更新日期：1974-03-01 00:00:00

abstract：:Human papillomavirus type 18 (HPV-18) infects genital squamous epithelium and is an etiological agent of cervical cancer. Cell-type-specific expression of HPV-18 is directed by the region upstream of the viral early genes that contains a transcriptional enhancer whose function is dependent solely on cellular factors. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack DH,Laimins LA

更新日期：1991-10-15 00:00:00

abstract：:In the course of scrapie, a transmissible spongiform encephalopathy caused by an unconventional agent, a normal cellular protein is converted to an abnormal form that copurifies with infectivity and aggregates to form deposits of amyloid. We have used immunocytochemistry and methods that enhance detection of amyloidog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diedrich JF,Bendheim PE,Kim YS,Carp RI,Haase AT

更新日期：1991-01-15 00:00:00

abstract：:It is possible, by using Fourier-transform infrared (FTIR) difference spectroscopy, to detect the conformational changes occurring in both the protein and the chromophore of bacteriorhodopsin during the photocycle. In contrast to Raman spectroscopy, a laser is unnecessary and hence the problem of a perturbing probe be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothschild KJ,Marrero H

更新日期：1982-07-01 00:00:00

abstract：:In 1950, Barbara McClintock published a Classic PNAS article, "The origin and behavior of mutable loci in maize," which summarized the evidence leading to her discovery of transposition. The article described a number of genome alterations revealed through her studies of the Dissociation locus, the first mobile geneti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fedoroff NV

更新日期：2012-12-11 00:00:00

abstract：:The nature of integrated viral DNA in normal and leukemic chicken cells has been studied by sequential nucleic acid hybridization procedures that localize the viral specific DNA in cellular DNA regions differing in reiteration frequency. First, DNA.DNA reassociation was employed to fractionate cellular DNA sequences a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans RM,Baluda MA,Shoyab M

更新日期：1974-08-01 00:00:00

abstract：:Ca(2+) signaling regulates cell function. This is subject to modulation by H(+) ions that are universal end-products of metabolism. Due to slow diffusion and common buffers, changes in cytoplasmic [Ca(2+)] ([Ca(2+)]i) or [H(+)] ([H(+)]i) can become compartmentalized, leading potentially to complex spatial Ca(2+)/H(+) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swietach P,Youm JB,Saegusa N,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2013-05-28 00:00:00

abstract：:Chromosome 3p and 1p deletions are among the most frequent genetic changes in human lung cancer and although candidate tumor suppressor genes have been identified in these regions, no causative correlations have been drawn between deletion or mutation of these and lung carcinogenesis. We identify XPC and Gadd45a as ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hollander MC,Philburn RT,Patterson AD,Velasco-Miguel S,Friedberg EC,Linnoila RI,Fornace AJ Jr

更新日期：2005-09-13 00:00:00

abstract：:The causes of major and rapid transitions observed in biological macroevolution as well as in the evolution of social systems are a subject of much debate. Here we identify the proximate causes of crashes and recoveries that arise dynamically in a model system in which populations of (molecular) species coevolve with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jain S,Krishna S

更新日期：2002-02-19 00:00:00

abstract：:We have synthesized pulmonary surfactant apoprotein SP-B peptides by solid-phase chemistry and demonstrated their ability to enhance the surface-active properties of synthetic lipid mixtures. The synthetic peptides were reactive with antiserum generated against the native bovine surfactant peptide. Both peptides confe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarin VK,Gupta S,Leung TK,Taylor VE,Ohning BL,Whitsett JA,Fox JL

更新日期：1990-04-01 00:00:00

abstract：:Spontaneous cross-linking in vitro, during incubation of purified collagen in nonstriated and native type fibrils, was studied to elucidate the relationship between intra and intermolecular cross-links. In nonbanded fibrils, the aldol condensation products of two allysyl residues, previously shown to constitute the in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang AH,Gross J

更新日期：1970-11-01 00:00:00

abstract：:We have totally synthesized a gene that codes for rat hepatic cytochrome b5. The 5' flanking region was designed for efficient expression of this gene in Escherichia coli by incorporating an optimum ribosome binding site and spacer region. Both a soluble form, analogous to the protease-treated microsomal protein, as w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beck von Bodman S,Schuler MA,Jollie DR,Sligar SG

更新日期：1986-12-01 00:00:00

abstract：:Gene regulation is a process with many steps allowing for stochastic biochemical reactions, which leads to expression noise-i.e., the cell-to-cell stochastic fluctuation in protein abundance. Such expression noise can give rise to drastically diverse phenotypes, even within isogenic cell populations. Although numerous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Min R,Vizeacoumar FJ,Jin K,Xin X,Zhang Z

更新日期：2010-06-08 00:00:00

abstract：:Arrestins are regulatory molecules for G-protein coupled receptor function. In visual rhodopsin, selective binding of arrestin to the cytoplasmic side of light-activated, phosphorylated rhodopsin (P-Rh*) terminates signaling via the G-protein transducin. While the "phosphate-sensor" of arrestin for the recognition of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirchberg K,Kim TY,Möller M,Skegro D,Dasara Raju G,Granzin J,Büldt G,Schlesinger R,Alexiev U

更新日期：2011-11-15 00:00:00

abstract：:A dinuclear nickel complex with methyl and thiolate ligands, Ni(dadt(Et))Ni(Me)(SDmp) (2), has been synthesized as a dinuclear Ni(d)-Ni(p)-site model of acetyl-CoA synthase (ACS) (dadt(Et) is N,N'-diethyl-3,7-diazanonane-1,9-dithiolate; Dmp is 2,6-dimesitylphenyl). Complex 2 was prepared via 2 methods: (i) ligand subs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ito M,Kotera M,Matsumoto T,Tatsumi K

更新日期：2009-07-21 00:00:00

abstract：:The essential eukaryotic pre-mRNA splicing factor U2AF (U2 small nuclear ribonucleoprotein auxiliary factor) is required to specify the 3' splice at an early step in spliceosome assembly. U2AF binds site-specifically to the intron polypyrimidine tract and recruits U2 small nuclear ribonucleoprotein to the branch site....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudner DZ,Kanaar R,Breger KS,Rio DC

更新日期：1996-09-17 00:00:00

abstract：:Attending to a stimulus is known to enhance the neural responses to that stimulus. Recent experiments on visual attention have shown that this modulation can have object-based characteristics, such that, when certain parts of a visual object are attended, other parts automatically also receive enhanced processing. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busse L,Roberts KC,Crist RE,Weissman DH,Woldorff MG

更新日期：2005-12-20 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal disease in critical need of new therapeutic strategies. Here, we report that the stress-inducible 78-kDa glucose-regulated protein (GRP78/HSPA5), a key regulator of endoplasmic reticulum homeostasis and PI3K/AKT signaling, is overexpressed in the acini an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen J,Ha DP,Zhu G,Rangel DF,Kobielak A,Gill PS,Groshen S,Dubeau L,Lee AS

更新日期：2017-05-16 00:00:00

abstract：:A half-century's worth of research has established the existence of numerous event-related potential components measuring different cognitive operations in humans including the selection of stimuli by covert attention mechanisms. Surprisingly, it is unknown whether nonhuman primates exhibit homologous electrophysiolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woodman GF,Kang MS,Rossi AF,Schall JD

更新日期：2007-09-18 00:00:00

abstract：:Cytochrome c oxidase, the terminal protein in the respiratory chain, converts oxygen into water and helps generate the electrochemical gradient used in the synthesis of ATP. The catalytic action of cytochrome c oxidase involves electron transfer, proton transfer, and O2 reduction. These events trigger specific molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nyquist RM,Heitbrink D,Bolwien C,Gennis RB,Heberle J

更新日期：2003-07-22 00:00:00

abstract：:Pyrimethamine (Pyr) targets dihydrofolate reductase of Plasmodium vivax (PvDHFR) as well as other malarial parasites, but its use as antimalarial is hampered by the widespread high resistance. Comparison of the crystal structures of PvDHFR from wild-type and the Pyr-resistant (SP21, Ser-58 --> Arg + Ser-117 --> Asn) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kongsaeree P,Khongsuk P,Leartsakulpanich U,Chitnumsub P,Tarnchompoo B,Walkinshaw MD,Yuthavong Y

更新日期：2005-09-13 00:00:00

abstract：:The formation of a fracture network is a key process for many geophysical and industrial practices from energy resource recovery to induced seismic management. We focus on the initial stage of a fracture network formation using experiments on the symmetric coalescence of two equal coplanar, fluid-driven, penny-shaped ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Keeffe NJ,Zheng Z,Huppert HE,Linden PF

更新日期：2018-10-09 00:00:00

abstract：:rap1/Krev-1 is a p21ras-related GTP-binding protein that has been implicated in the reversion of the ras-transformed cell phenotype. We have identified a GTPase-activating protein (GAP) specific for rap in plasma membranes isolated from differentiated HL60 cells. The rap GAP activity remained quantitatively associated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polakis PG,Rubinfeld B,Evans T,McCormick F

更新日期：1991-01-01 00:00:00

abstract：:Monospecific antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a BALB/c mouse immunized with rat skeletal myofibrils. After cloning 3 times on agarose, two stable clones were obtained and chosen for further characterization. The first clone, JLB1, produced an antibody that recogniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin JJ

更新日期：1981-04-01 00:00:00

abstract：:The cofactor composition and electron-transfer kinetics of the reaction center (RC) from a magnesium chelatase (bchD) mutant of Rhodobacter sphaeroides were characterized. In this RC, the special pair (P) and accessory (B) bacteriochlorophyll (BChl) -binding sites contain Zn-BChl rather than BChl a. Spectroscopic meas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin S,Jaschke PR,Wang H,Paddock M,Tufts A,Allen JP,Rosell FI,Mauk AG,Woodbury NW,Beatty JT

更新日期：2009-05-26 00:00:00

abstract：:An excised patch of embryonic muscle membrane was used as a probe for measuring the release of acetylcholine (AcCho) from growing spinal neurons in Xenopus cell culture. The neuron was stimulated extracellularly at the soma, and the evoked AcCho release was monitored at the growth cone, along the neurite, and near the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun YA,Poo MM

更新日期：1987-04-01 00:00:00

abstract：:A library of Photobacterium phosphoreum DNA was screened in lambda 2001 for the lumazine protein gene, using two degenerate 17-mer oligonucleotide probes that were deduced from a partial protein primary sequence. The lumazine protein gene was localized to a 3.4-kilobase BamHI/EcoRI fragment in one clone. The fragment ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Kane DJ,Woodward B,Lee J,Prasher DC

更新日期：1991-02-15 00:00:00

abstract：:Several findings suggest that signals from tyrosine kinases are transduced, at least in part, through ras proteins. These findings include (i) blockage of the transforming activity of constitutively active tyrosine kinases by inhibiting ras function and (ii) genetic screens in Caenorhabditis elegans and in Drosophila ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowtell D,Fu P,Simon M,Senior P

更新日期：1992-07-15 00:00:00