Abstract:
:In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis of these isoforms, we have sequenced a 5.8-kilobase region of the Drosophila genome that contains the entire Gpdh locus. Primer-extension and RNase protection assays show that the gene consists of eight exons and has a single transcription-start point. RNase protection mapping and comparison of the genomic sequence from three different cDNA clones reveal that three protein isoforms of glycerol-3-phosphate dehydrogenase are produced by alternative processing of 3' exons. Two of the isoforms differ from the third by the addition of either three or ten amino acids to their C-terminal ends. Transcripts corresponding to two of the isoforms are expressed during both larval and adult stages, while the third isoform is produced only in adults.
journal_name
Proc Natl Acad Sci U S Aauthors
von Kalm L,Weaver J,DeMarco J,MacIntyre RJ,Sullivan DTdoi
10.1073/pnas.86.13.5020subject
Has Abstractpub_date
1989-07-01 00:00:00pages
5020-4issue
13eissn
0027-8424issn
1091-6490journal_volume
86pub_type
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