Ryanodine receptor leak mediated by caspase-8 activation leads to left ventricular injury after myocardial ischemia-reperfusion.

abstract：:In recent years, advertisers and magazine editors have been widely criticized for taking digital photo retouching to an extreme. Impossibly thin, tall, and wrinkle- and blemish-free models are routinely splashed onto billboards, advertisements, and magazine covers. The ubiquity of these unrealistic and highly idealize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kee E,Farid H

更新日期：2011-12-13 00:00:00

abstract：:Aotus monkeys, which are generally killed when infected with the human malaria parasite Plasmodium falciparum, have been identified and grouped by karyotype. These animals were immunized with parasite material obtained from P. falciparum cultivated in vitro which had been maintained in culture for over a year. When su...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reese RT,Trager W,Jensen JB,Miller DA,Tantravahi R

更新日期：1978-11-01 00:00:00

abstract：:The rearrangement of immunoglobulin genes in B-lymphocyte precursors requires the expression of the recombination activating genes (Rag), which leads to the generation of a highly diverse B-cell repertoire. We can use the level of Rag-1 mRNA in the bone marrow as an index of its capacity to support the maturation of B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ben-Yehuda A,Szabo P,Dyall R,Weksler ME

更新日期：1994-12-06 00:00:00

abstract：:Phosducin is a soluble phosphoprotein found in retinal photoreceptor cells and in the pineal gland. It binds to the beta gamma subunits of guanine nucleotide-binding proteins (G proteins) (G beta gamma) and may regulate G-protein function. In this study, the ability of specific regions of phosducin to bind G beta gamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Wu D,Slepak VZ,Simon MI

更新日期：1995-03-14 00:00:00

abstract：:Interferon regulatory factor (IRF) 4 is a member of the IRF family of transcription factors and plays critical roles in the development of CD4(+) T cells into Th2 and Th17 cells. Using the infection model of Nippostrongyrus brasiliensis, we have confirmed the critical roles of IRF-4 in Th2 development in vivo by using...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honma K,Kimura D,Tominaga N,Miyakoda M,Matsuyama T,Yui K

更新日期：2008-10-14 00:00:00

abstract：:Thyroid hormone (TH) metabolism, mediated by deiodinase types 1, 2, and 3 (D1, D2, and D3) is profoundly affected by acute illness. We examined the role of TH metabolism during ventilator-induced lung injury (VILI) in mice. Mice exposed to VILI recapitulated the serum TH findings of acute illness, namely a decrease in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barca-Mayo O,Liao XH,DiCosmo C,Dumitrescu A,Moreno-Vinasco L,Wade MS,Sammani S,Mirzapoiazova T,Garcia JG,Refetoff S,Weiss RE

更新日期：2011-12-06 00:00:00

abstract：:We have developed a color complementation assay that allows rapid screening of specific genomic DNA sequences. It is based on the simultaneous amplification of two or more DNA segments with fluorescent oligonucleotide primers such that the generation of a color, or combination of colors, can be visualized and used for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chehab FF,Kan YW

更新日期：1989-12-01 00:00:00

abstract：:A theory is proposed to study the sensitivity of cylindrical explosives. Using the equations governing the conservation of mass, energy, and momentum, the equation of state, and Newtonian mechanics, we have derived the expression D = D(o)e(-a2/d), in which the apparent length of the reaction zone, a, takes into accoun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eyring H,Walker FE,Ma SM,Coon N

更新日期：1980-05-01 00:00:00

abstract：:Src homology 2 (SH2) domains are phosphotyrosine-binding modules found within various signal-transducing proteins. We have determined by 125I competition assay and surface plasmon resonance that the SH2 domains of Src and Lck bind to a variety of phosphopeptides with similar affinity and specificity. Both bound with h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Payne G,Shoelson SE,Gish GD,Pawson T,Walsh CT

更新日期：1993-06-01 00:00:00

abstract：:We have identified the mutation responsible for the autosomal recessive wasted (wst) mutation of the mouse. Wasted mice are characterized by wasting and neurological and immunological abnormalities starting at 21 days after birth; they die by 28 days. A deletion of 15.8 kb in wasted mice abolishes expression of a gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chambers DM,Peters J,Abbott CM

更新日期：1998-04-14 00:00:00

abstract：:We present here the complete genome sequence of a common avian clone of Pasteurella multocida, Pm70. The genome of Pm70 is a single circular chromosome 2,257,487 base pairs in length and contains 2,014 predicted coding regions, 6 ribosomal RNA operons, and 57 tRNAs. Genome-scale evolutionary analyses based on pairwise...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： May BJ,Zhang Q,Li LL,Paustian ML,Whittam TS,Kapur V

更新日期：2001-03-13 00:00:00

abstract：:The distribution of actin stress fibers in normal and regenerating (after endothelial denudation by means of a balloon catheter) rabbit aortic endothelial cells has been studied by means of immunofluorescence with human actin autoantibodies on en face endothelial cell preparations. Our results show that: (i) under nor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gabbiani G,Gabbiani F,Lombardi D,Schwartz SM

更新日期：1983-04-01 00:00:00

abstract：:Mammalian class A macrophage-specific scavenger receptors (SR-A) exhibit unusually broad binding specificity for a wide variety of polyanionic ligands. The properties of these receptors suggest that they may be involved in atherosclerosis and host defense. We have previously observed a similar receptor activity in Dro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearson A,Lux A,Krieger M

更新日期：1995-04-25 00:00:00

abstract：:CD95 type I and II cells differ in their dependence on mitochondria to execute apoptosis, because antiapoptotic members of the Bcl-2 family render only type II cells resistant to death receptor-induced apoptosis. They can also be distinguished by a more efficient formation of the death-inducing signaling complex in ty...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Algeciras-Schimnich A,Pietras EM,Barnhart BC,Legembre P,Vijayan S,Holbeck SL,Peter ME

更新日期：2003-09-30 00:00:00

abstract：:The cationic conductances of purified bovine retinal rod membranes were studied by incorporation of vesicles into planar lipid bilayers. When the membranes were stripped of all peripheral proteins [guanine nucleotide-binding protein (G protein) and cGMP phosphodiesterase (3',5'-cyclic-GMP 5'-nucleotidohydrolase), EC 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett N,Ildefonse M,Crouzy S,Chapron Y,Clerc A

更新日期：1989-05-01 00:00:00

abstract：:Mutations in genes encoding membrane proteins have been associated with cell death of unknown cause from invertebrate development to human degenerative diseases. A point mutation in the gene for myelin proteolipid protein (PLP) underlies oligodendrocyte death and dysmyelination in jimpy mice, an accurate model for Pel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneider AM,Griffiths IR,Readhead C,Nave KA

更新日期：1995-05-09 00:00:00

abstract：:To study the behavior of hematopoietic stem cells in vivo, we transplanted glucose-6-phosphate dehydrogenase (G6PD) heterozygous (female Safari) cats with small amounts of autologous marrow. The G6PD phenotypes of erythroid burst-forming units and granulocyte/macrophage colony-forming units were repeatedly assayed for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abkowitz JL,Persik MT,Shelton GH,Ott RL,Kiklevich JV,Catlin SN,Guttorp P

更新日期：1995-03-14 00:00:00

abstract：:A computational model is presented that can be used as a tool in the design of safer chemicals. This model predicts the rate of hydrogen-atom abstraction by cytochrome P450 enzymes. Excellent correlations between biotransformation rates and the calculated activation energies (delta Hact) of the cytochrome P450-mediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yin H,Anders MW,Korzekwa KR,Higgins L,Thummel KE,Kharasch ED,Jones JP

更新日期：1995-11-21 00:00:00

abstract：:The biosynthesis and endocrine regulation of sex pheromone production in the female German cockroach (Blattella germanica) were determined. Radio-TLC and radio-GLC were used to demonstrate the metabolism of 3,11-dimethylnonacosane, a major cuticular lipid component, to the corresponding alkan-2-ol and methyl ketone. [...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chase J,Touhara K,Prestwich GD,Schal C,Blomquist GJ

更新日期：1992-07-01 00:00:00

abstract：:There is currently an unmet need for versatile techniques to monitor the assembly and dynamics of ternary complexes in live cells. Here we describe bioluminescence resonance energy transfer with fluorescence enhancement by combined transfer (BRETFect), a high-throughput technique that enables robust spectrometric dete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cotnoir-White D,El Ezzy M,Boulay PL,Rozendaal M,Bouvier M,Gagnon E,Mader S

更新日期：2018-03-13 00:00:00

abstract：:A complete experimental format is given for the reconstitution of human hemoglobin from the separated heme-free alpha- and beta-globin chains (alpha degrees, beta degrees) and hemin, by two alternative routes. Based on their oxygen binding properties, the reaction of the ferri-forms with reducing agent, and the respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yip YK,Waks M,Beychok S

更新日期：1977-01-01 00:00:00

abstract：:The role of ornithine decarboxylase (OrnDCase, EC 4.1.1.17) and of the polyamines [putrescine (Put), spermidine (Spd), and spermine (Spm)] in mouse skin tumor promotion was investigated by the use of alpha-difluoromethylornithine (CHF2-Orn), an enzyme-activated irreversible inhibitor of OrnDCase. 12-O-Tetradecanoylpho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weeks CE,Herrmann AL,Nelson FR,Slaga TJ

更新日期：1982-10-01 00:00:00

abstract：:Purified dicyclohexylcarbodiimide-sensitive ATPase (TF0-F1) from thermophilic bacterium PS3 is composed of a water soluble part with ATP hydrolytic activity (TF1) and a water insoluble moiety (TF0). All of the five subunits (alpha, beta, gamma, delta, and epsilon) of TF1 were isolated. TF1 was reconstituted from the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida M,Okamoto H,Sone N,Hirata H,Kagawa Y

更新日期：1977-03-01 00:00:00

abstract：:Evolutionary theory reveals that punishment is effective in promoting cooperation and maintaining social norms. Although it is accepted that emotions are connected to punishment decisions, there remains substantial debate over why humans use costly punishment. Here we show experimentally that constraints on emotion ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao E,Houser D

更新日期：2005-05-17 00:00:00

abstract：:The acetylcholine receptor is a protein that contains certain critical disulfide bonds. Experiments were designed to determine the role such bonds might play in the physiological activity of the receptor. Modification of the receptor with sodium bisulfite and diamide produced an increase in the time constants of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steinacker A,Zuazaga DC

更新日期：1981-12-01 00:00:00

abstract：:Cell migration and spreading involve the coordination of membrane trafficking, actomyosin contraction, and modifications to plasma membrane tension and area. The biochemical or biophysical basis for this coordination is however unknown. In this study, we show that during cell spreading, lamellipodia protrusion flatten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gauthier NC,Fardin MA,Roca-Cusachs P,Sheetz MP

更新日期：2011-08-30 00:00:00

abstract：:Prion diseases are characterized by an accumulation of PrP(Sc), a misfolded isoform of the normal cellular prion protein, PrP(C). We previously reported the bioactivity of acridine-based compounds against PrP(Sc) replication in scrapie-infected neuroblastoma cells and now report the improved potency of bis-acridine co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： May BC,Fafarman AT,Hong SB,Rogers M,Deady LW,Prusiner SB,Cohen FE

更新日期：2003-03-18 00:00:00

abstract：:A method is described for the production of hybridoma cell lines secreting antibodies directed against the polyoma virus tumor antigens (T-Ags). Ten cloned lines have been established by this procedure and designated alpha Py Cl and C4, with activities against all three T-Ags; alpha Py LT1, -4 and -7, with activities ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dilworth SM,Griffin BE

更新日期：1982-02-01 00:00:00

abstract：:Early passages of NIH 3T3 cells yield about 10 transformed foci for every 10(5) cells seeded after the cells multiply to confluence in a standardized 2-week assay. The question arose whether more cells would give rise to foci if given more time for their development. This question could not be answered simply by exten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow M,Yao A,Rubin H

更新日期：1994-01-18 00:00:00

abstract：:Micrometeorites with diameter approximately 100-200 microm dominate the flux of extraterrestrial matter on Earth. The vast majority of micrometeorites are chemically, mineralogically, and isotopically related to carbonaceous chondrites, which amount to only 2.5% of meteorite falls. Here, we report the discovery of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gounelle M,Chaussidon M,Morbidelli A,Barrat JA,Engrand C,Zolensky ME,McKeegan KD

更新日期：2009-04-28 00:00:00