Abstract:
:Two hepatoma cell lines were incubated for 72 h with ATRA and its analog 13cisRA and according to MTT assay, Hep3B cells were highly susceptible whereas HepG2 cells were more resistant to the treatment. At the high concentration of 166 microM, retinoids were able to induce apoptosis in both cell lines and the highest effect was observed in HepG2 cells treated with ATRA. TUNEL-based photometric ELISA showed that at the same retinoid concentration tested by flow cytometry, both cell lines showed apoptosis whereas plasma membranes were not significantly disrupted. Inhibitors of apoptosis Bcl-xL and survivin were downregulated in Hep3B cells by treatment with both retinoids. Bax, a pro-apoptotic protein, was not significantly upregulated in Hep3B cells, but was slightly increased in HepG2 cells treated with 13cisRA. Both procaspase-3 and procaspase-8 were cleaved in Hep3B cells, suggesting apoptosis could be triggered through the extrinsic pathway. In the case of HepG2 cells, lack of caspase activation suggests a mechanism dependent on other kind of proteases.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Arce F,Gätjens-Boniche O,Vargas E,Valverde B,Díaz Cdoi
10.1016/j.canlet.2005.06.047keywords:
subject
Has Abstractpub_date
2005-11-18 00:00:00pages
271-81issue
2eissn
0304-3835issn
1872-7980pii
S0304-3835(05)00672-5journal_volume
229pub_type
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