Abstract:
:The DNA of functional cis-regulatory modules displays extensive sequence conservation in comparisons of genomes from modestly distant species. Patches of sequence that are several hundred base pairs in length within these modules are often seen to be 80-95% identical, although the flanking sequence cannot even be aligned. However, it is unlikely that base pairs located between the transcription factor target sites of cis-regulatory modules have sequence-dependent function, and the mechanism that constrains evolutionary change within cis-regulatory modules is incompletely understood. We chose five functionally characterized cis-regulatory modules from the Strongylocentrotus purpuratus (sea urchin) genome and obtained orthologous regulatory and flanking sequences from a bacterial artificial chromosome genome library of a congener, Strongylocentrotus franciscanus. As expected, single-nucleotide substitutions and small indels occur freely at many positions within the regulatory modules of these two species, as they do outside the regulatory modules. However, large indels (>20 bp) are statistically almost absent within the regulatory modules, although they are common in flanking intergenic or intronic sequence. The result helps to explain the patterns of evolutionary sequence divergence characteristic of cis-regulatory DNA.
journal_name
Proc Natl Acad Sci U S Aauthors
Cameron RA,Chow SH,Berney K,Chiu TY,Yuan QA,Krämer A,Helguero A,Ransick A,Yun M,Davidson EHdoi
10.1073/pnas.0505291102keywords:
subject
Has Abstractpub_date
2005-08-16 00:00:00pages
11769-74issue
33eissn
0027-8424issn
1091-6490pii
0505291102journal_volume
102pub_type
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