Abstract:
:The glycolipid alpha-galactosylceramide binds with high affinity to CD1d and stimulates natural killer T cells. Here we report the crystal structure of human CD1d in complex with synthetic alpha-galactosylceramide at a resolution of 3.0 A. The structure shows a tightly fit lipid in the CD1d binding groove, with the sphingosine chain bound in the C' pocket and the longer acyl chain anchored in the A' pocket. We also present the CD1d structure without lipid, which has a more open conformation of the binding groove, suggesting a dual conformation of CD1d in which the 'open' conformation is more able to load lipids. These structures provide clues as to how CD1 molecules load glycolipids as well as data to guide the design of new therapeutic agents.
journal_name
Nat Immunoljournal_title
Nature immunologyauthors
Koch M,Stronge VS,Shepherd D,Gadola SD,Mathew B,Ritter G,Fersht AR,Besra GS,Schmidt RR,Jones EY,Cerundolo Vdoi
10.1038/ni1225keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
819-26issue
8eissn
1529-2908issn
1529-2916pii
ni1225journal_volume
6pub_type
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journal_title:Nature immunology
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pub_type: 杂志文章,已发布勘误
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更新日期:2000-09-01 00:00:00
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更新日期:2015-03-01 00:00:00
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journal_title:Nature immunology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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更新日期:2014-10-01 00:00:00
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journal_title:Nature immunology
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:Nature immunology
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