Abstract:
:microRNAs (miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes. Primary miRNA (pri-miRNA) transcripts are transformed to mature miRNA by the successive actions of two RNase III endonucleases. Drosha converts pri-miRNA transcripts to precursor miRNA (pre-miRNA); Dicer, in turn, converts pre-miRNA to mature miRNA. Here, we show that normal processing of Drosophila pre-miRNAs by Dicer-1 requires the double-stranded RNA-binding domain (dsRBD) protein Loquacious (Loqs), a homolog of human TRBP, a protein first identified as binding the HIV trans-activator RNA (TAR). Efficient miRNA-directed silencing of a reporter transgene, complete repression of white by a dsRNA trigger, and silencing of the endogenous Stellate locus by Suppressor of Stellate, all require Loqs. In loqs(f00791) mutant ovaries, germ-line stem cells are not appropriately maintained. Loqs associates with Dcr-1, the Drosophila RNase III enzyme that processes pre-miRNA into mature miRNA. Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Förstemann K,Tomari Y,Du T,Vagin VV,Denli AM,Bratu DP,Klattenhoff C,Theurkauf WE,Zamore PDdoi
10.1371/journal.pbio.0030236keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
e236issue
7eissn
1544-9173issn
1545-7885pii
05-PLBI-RA-0205R1journal_volume
3pub_type
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