Abstract:
:Bacteriophage modulation of microbial populations impacts critical processes in ocean, soil, and animal ecosystems. However, the role of bacteriophages with RNA genomes (RNA bacteriophages) in these processes is poorly understood, in part because of the limited number of known RNA bacteriophage species. Here, we identify partial genome sequences of 122 RNA bacteriophage phylotypes that are highly divergent from each other and from previously described RNA bacteriophages. These novel RNA bacteriophage sequences were present in samples collected from a range of ecological niches worldwide, including invertebrates and extreme microbial sediment, demonstrating that they are more widely distributed than previously recognized. Genomic analyses of these novel bacteriophages yielded multiple novel genome organizations. Furthermore, one RNA bacteriophage was detected in the transcriptome of a pure culture of Streptomyces avermitilis, suggesting for the first time that the known tropism of RNA bacteriophages may include gram-positive bacteria. Finally, reverse transcription PCR (RT-PCR)-based screening for two specific RNA bacteriophages in stool samples from a longitudinal cohort of macaques suggested that they are generally acutely present rather than persistent.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Krishnamurthy SR,Janowski AB,Zhao G,Barouch D,Wang Ddoi
10.1371/journal.pbio.1002409subject
Has Abstractpub_date
2016-03-24 00:00:00pages
e1002409issue
3eissn
1544-9173issn
1545-7885pii
PBIOLOGY-D-15-03167journal_volume
14pub_type
杂志文章相关文献
PLOS BIOLOGY文献大全abstract::Negative regulation of receptor signaling is essential for controlling cell activation and differentiation. In B-lymphocytes, the down-regulation of B-cell antigen receptor (BCR) signaling is critical for suppressing the activation of self-reactive B cells; however, the mechanism underlying the negative regulation of ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001704
更新日期:2013-11-01 00:00:00
abstract::Eukaryotic cells respond to accumulation of unfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR), a signal transduction pathway that communicates between the ER and the nucleus. In yeast, a large set of UPR target genes has been experimentally determined, but the previo...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020246
更新日期:2004-08-01 00:00:00
abstract::Mimivirus is the largest known virus whose genome and physical size are comparable to some small bacteria, blurring the boundary between a virus and a cell. Structural studies of Mimivirus have been difficult because of its size and long surface fibers. Here we report the use of enzymatic digestions to remove the surf...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000092
更新日期:2009-04-28 00:00:00
abstract::The relative contributions to modern European populations of Paleolithic hunter-gatherers and Neolithic farmers from the Near East have been intensely debated. Haplogroup R1b1b2 (R-M269) is the commonest European Y-chromosomal lineage, increasing in frequency from east to west, and carried by 110 million European men....
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000285
更新日期:2010-01-19 00:00:00
abstract::Flexible behavior is critical for everyday decision-making and has been implicated in restricted, repetitive behaviors (RRB) in autism spectrum disorder (ASD). However, how flexible behavior changes developmentally in ASD remains largely unknown. Here, we used a developmental approach and examined flexible behavior on...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000908
更新日期:2020-10-27 00:00:00
abstract::Mast cells are implicated in the pathogenesis of inflammatory and autoimmune diseases. However, this notion based on studies in mast cell-deficient mice is controversial. We therefore established an in vivo model for hyperactive mast cells by specifically ablating the NF-κB negative feedback regulator A20. While A20 d...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001762
更新日期:2014-01-01 00:00:00
abstract::Although the developmental principles of sensory and cognitive processing have been extensively investigated, their synergy has been largely neglected. During early life, most sensory systems are still largely immature. As a notable exception, the olfactory system is functional at birth, controlling mother-offspring i...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2006994
更新日期:2019-01-31 00:00:00
abstract::Obesity is associated with changes in the plasma lipids. Although simple lipid quantification is routinely used, plasma lipids are rarely investigated at the level of individual molecules. We aimed at predicting different measures of obesity based on the plasma lipidome in a large population cohort using advanced mach...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000443
更新日期:2019-10-18 00:00:00
abstract::The anaerobic bacterium Fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of Na⁺. Here, we demonstrate that this ion-motive force is directly coupled to ATP synthesis, via an F₁F₀-ATP synthase with a novel Na⁺ recognition motif, shared by other human pathogens. Molecular model...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001596
更新日期:2013-01-01 00:00:00
abstract::In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Should one apply chemical mutagenesis, and do strains with high innate m...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000182
更新日期:2019-03-29 00:00:00
abstract::Patterned spontaneous activity in the developing retina is necessary to drive synaptic refinement in the lateral geniculate nucleus (LGN). Using perforated patch recordings from neurons in LGN slices during the period of eye segregation, we examine how such burst-based activity can instruct this refinement. Retinogeni...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0050061
更新日期:2007-03-01 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pbio.1002436.]. ...
journal_title:PLoS biology
pub_type: 已发布勘误
doi:10.1371/journal.pbio.1002494
更新日期:2016-06-08 00:00:00
abstract::Developmental biology (including embryology) is proposed as "the stem cell of biological disciplines." Genetics, cell biology, oncology, immunology, evolutionary mechanisms, neurobiology, and systems biology each has its ancestry in developmental biology. Moreover, developmental biology continues to roll on, budding o...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2003691
更新日期:2017-12-28 00:00:00
abstract::Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to-or is selected by-this environment remains unclear. From previous studies, the parasites that cause...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2004328
更新日期:2018-03-12 00:00:00
abstract::The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfol...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000102
更新日期:2009-05-01 00:00:00
abstract::During gestation, uterine smooth muscle cells transition from a state of quiescence to one of contractility, but the molecular mechanisms underlying this transition at a genomic level are not well-known. To better understand these events, we evaluated the epigenetic landscape of the mouse myometrium during the pregnan...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000710
更新日期:2020-07-15 00:00:00
abstract::HAPLESS2 (HAP2) is a broadly conserved, gamete-expressed transmembrane protein that was shown recently to be structurally homologous to viral class II fusion proteins, which initiate fusion with host cells via insertion of fusion loops into the host membrane. However, the functional conformation of the HAP2 fusion loo...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2006357
更新日期:2018-08-13 00:00:00
abstract::Rapamycin (Rap) and its derivatives, called rapalogs, are being explored in clinical trials targeting cancer and neurodegeneration. The underlying mechanisms of Rap actions, however, are not well understood. Mechanistic target of rapamycin (mTOR), a lysosome-localized protein kinase that acts as a critical regulator o...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000252
更新日期:2019-05-21 00:00:00
abstract::What do "microbes" have to do with social equity? These microorganisms are integral to our health, that of our natural environment, and even the "health" of the environments we build. The loss, gain, and retention of microorganisms-their flow between humans and the environment-can greatly impact our health. It is well...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000536
更新日期:2019-11-26 00:00:00
abstract::One of the major challenges of functional genomics is to unravel the connection between genotype and phenotype. So far no global analysis has attempted to explore those connections in the light of the large phenotypic variability seen in nature. Here, we use an unsupervised, systematic approach for associating genes a...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030134
更新日期:2005-05-01 00:00:00
abstract::[This corrects the article DOI: 10.1371/journal.pbio.1001326.]. ...
journal_title:PLoS biology
pub_type: 杂志文章,已发布勘误
doi:10.1371/journal.pbio.1002616
更新日期:2017-12-11 00:00:00
abstract::Although psychological and computational models of time estimation have postulated the existence of neural representations tuned for specific durations, empirical evidence of this notion has been lacking. Here, using a functional magnetic resonance imaging (fMRI) adaptation paradigm, we show that the inferior parietal...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002262
更新日期:2015-09-17 00:00:00
abstract::The COVID-19 pandemic has motivated many open and collaborative analytical research projects with real-world impact. However, despite their value, such activities are generally overlooked by traditional academic metrics. Science is ultimately improved by analytical work, whether ensuring reproducible and well-document...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000913
更新日期:2020-10-16 00:00:00
abstract::From early dinosaurs with as many as nine wrist bones, modern birds evolved to develop only four ossifications. Their identity is uncertain, with different labels used in palaeontology and developmental biology. We examined embryos of several species and studied chicken embryos in detail through a new technique allowi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001957
更新日期:2014-09-30 00:00:00
abstract::From a research perspective, the interest in biobanking continues to intensify. Governments and industry have invested heavily in biobanks, as exemplified by initiatives like the United Kingdom Biobank and United States' Precision Medicine Initiative. But despite this enthusiasm, many profound legal and ethical challe...
journal_title:PLoS biology
pub_type: 杂志文章,评审
doi:10.1371/journal.pbio.2002654
更新日期:2017-07-25 00:00:00
abstract::Three-dimensional (3D) structural information on many length scales is of central importance in biological research. Excellent methods exist to obtain structures of molecules at atomic, organelles at electron microscopic, and tissue at light-microscopic resolution. A gap exists, however, when 3D tissue structure needs...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020329
更新日期:2004-11-01 00:00:00
abstract::The signals that initiate cell invasion are not well understood, but there is increasing evidence that extracellular physical signals play an important role. Here we show that epithelial cell invasion in the intestine of zebrafish meltdown (mlt) mutants arises in response to unregulated contractile tone in the surroun...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001386
更新日期:2012-01-01 00:00:00
abstract::Reproducibility and transparency in biomedical sciences have been called into question, and scientists have been found wanting as a result. Putting aside deliberate fraud, there is evidence that a major contributor to lack of reproducibility is insufficient quality assurance of reagents used in preclinical research. C...
journal_title:PLoS biology
pub_type: 评论,杂志文章
doi:10.1371/journal.pbio.1002477
更新日期:2016-06-14 00:00:00
abstract::The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin recepto...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000901
更新日期:2020-11-02 00:00:00
abstract::Several studies have demonstrated that poor early nutrition, followed by growth compensation, can have negative consequences later in life. However, it remains unclear whether this is attributable to the nutritional deficit itself or a cost of compensatory growth. This distinction is important to our understanding bot...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0040251
更新日期:2006-07-01 00:00:00