Abstract:
:Angiogenesis is a crucial process in tissue remodeling during growth, both in the embryo and the adult. In our study we concentrated on the direct effect of beta-carotene on human umbilical cord originating from endothelial progenitor cells (EPCs). beta-Carotene uptake by EPCs was measured using a HPLC method. The determination of cell surface antigens was performed by flow cytometry. The effect on cell proliferation was estimated by measuring bromo-deoxyuridine incorporation. The influence on the formation of a tubular-like structure was investigated in a 3D assay in matrigel. Quantitative gene expression was estimated using real-time PCR. We demonstrated that beta-carotene in the physiological range of concentrations found in human blood is a potent activator of EPC chemotaxis, which is accompanied by a change in the expression of genes mediating cell adhesion and homing, but does not activate the final markers of endothelial differentiation. This study points to the prochemotactic and homing activity of beta-carotene in undifferentiated endothelial cell progenitors for the first time, which may suggest a potential role of this carotenoid in progenitor cell therapy aimed at angiogenesis and tissue repair.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Kieć-Wilk B,Polus A,Grzybowska J,Mikołajczyk M,Hartwich J,Pryjma J,Skrzeczyńska J,Dembińska-Kieć Adoi
10.1515/CCLM.2005.087keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
488-98issue
5eissn
1434-6621issn
1437-4331journal_volume
43pub_type
杂志文章abstract::Background Non-small cell lung cancer (NSCLC) patients benefit from targeted therapies both in first- and second-line treatment. Nevertheless, molecular profiling of lung cancer tumors after first disease progression is seldom performed. The analysis of circulating tumor DNA (ctDNA) enables not only non-invasive bioma...
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更新日期:2016-09-01 00:00:00
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更新日期:2015-02-01 00:00:00
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更新日期:2006-01-01 00:00:00
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更新日期:2009-01-01 00:00:00
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更新日期:2010-12-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章
doi:10.1515/CCLM.1998.096
更新日期:1998-08-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
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doi:10.1515/CCLM.1998.086
更新日期:1998-08-01 00:00:00
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