High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference.

abstract：BACKGROUND:This study compared automated vs. manual tissue grinding in terms of RNA yield obtained from oral mucosa biopsies. METHODS:A total of 20 patients undergoing uvulectomy for sleep-related disorders and 10 patients undergoing biopsy for head and neck squamous cell carcinoma were enrolled in the study. Samples ...

journal_title：Clinical chemistry and laboratory medicine

authors： Van der Vorst S,Dekairelle AF,Irenge L,Hamoir M,Robert A,Gala JL

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:The identification of human mitochondrial DNA (mtDNA) sequence variations, especially single nucleotide polymorphisms (SNPs), is important for many applications. The PCR-ligase detection reaction (LDR) method can reduce false-positives and eliminate the need for both post-PCR and post-ligation purifications ...

journal_title：Clinical chemistry and laboratory medicine

authors： Luo Y,Tang S,Gao W,Chen L,Yang X,Huang T,Gao Y

更新日期：2010-04-01 00:00:00

abstract：BACKGROUND:The aim of our study was to determine the frequency of anti-thyroid-stimulating hormone (TSH) receptor antibodies (TRAb) in Tunisian patients with Graves' disease (GD) and to compare the validity of TRAb to that of thyroperoxidase (TPO-Ab) and thyroglobulin antibodies (TG-Ab). METHODS:ELISA was used to dete...

journal_title：Clinical chemistry and laboratory medicine

authors： Mankaï A,Toumi D,Chadli-Chaieb M,Ghedira-Besbes L,Saad F,Ouertani M,Sfar H,Jeddi M,Chaieb L,Ghedira I

更新日期：2007-01-01 00:00:00

abstract：:The non-forage feeding of dairy cows rich in fast absorption carbohydrates, the low value of their euglycemic-hyperinsulinemic clamp suggest a dysregulation of carbohydrate metabolism able to produce neurodegenerative disorders. Comparisons between Alzheimer's disease developed in diabetes mellitus and bovine spongifo...

journal_title：Clinical chemistry and laboratory medicine

authors： Frey J

更新日期：2002-02-01 00:00:00

abstract：BACKGROUND:Established general risk score models for intensive care patients incorporate several clinical and laboratory data. However, the collection, documentation and classification of clinical data are time-consuming, incur labor-related costs, and are dependent on the experience of the examiner. Therefore, in the ...

journal_title：Clinical chemistry and laboratory medicine

authors： Stachon A,Segbers E,Hering S,Kempf R,Holland-Letz T,Krieg M

更新日期：2008-01-01 00:00:00

abstract：:A software has been developed on quality control, department economics, information supervise and personal administration. In this article, we discuss the part of quality control, from which we benefit most, on developing ideas, running principle, and application. This network will enable the implementation of total q...

journal_title：Clinical chemistry and laboratory medicine

authors： Yulong C

更新日期：2001-12-01 00:00:00

abstract：BACKGROUND:Pertussis has been increasing in frequency and can cause protean manifestation in infants, often resulting in extensive laboratory evaluation. METHODS:We examined the prevalence of cerebrospinal fluid (CSF) anomalies in infants with pertussis and parapertussis over a 5-year period. RESULTS:In total, 239 of...

journal_title：Clinical chemistry and laboratory medicine

authors： Chu TT,Groh J,Cruz AT

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND:Apolipoprotein E (APOE) and choline acetyltransferase (ChAT) have been suggested as candidate genes for determining the risk of late-onset Alzheimer's disease. The aim of this study was to simultaneously detect polymorphisms in codons 112 and 158 of APOE and codon 2 of ChAT by hybridization probe multiplexin...

journal_title：Clinical chemistry and laboratory medicine

authors： Park HD,Park KU,Kim KW,Song J,Chang HE,Heo SR,Lee HJ,Kim JQ

更新日期：2007-01-01 00:00:00

abstract：:Human C-reactive protein is a Ca2+-binding acute phase-protein with binding specificity for phosphocholine. Recent crystallographic and mutagenesis studies have provided a solid understanding of the structural biology of the protein, while experiments using transgenic mice have confirmed its host-defense function. The...

journal_title：Clinical chemistry and laboratory medicine

authors： Szalai AJ,Agrawal A,Greenhough TJ,Volanakis JE

更新日期：1999-03-01 00:00:00

abstract：:The mutant of CYP2D6*3 allele with A2637 deletion in exon 5 and the mutant of CYP2D6*4 allele G1934-->A, splice site defect are among the most common polymorphic alleles of CYP2D6 gene, resulting in a decreased or no activity of CYP isoenzyme. In this study, a reliable polymerase chain reaction-restriction fragment le...

journal_title：Clinical chemistry and laboratory medicine

authors： Topić E,Stefanović M,Nikolić V,Zoricić I,Ivanisević AM,Zuntar I

更新日期：1998-08-01 00:00:00

abstract：:Although prostate-specific antigen (PSA), or human kallikrein 3, is the most valuable tool available for the diagnosis and management of prostate cancer, as currently used it is insufficiently sensitive and specific for early detection or staging of the malignancy. Many new concepts have been introduced in order to op...

journal_title：Clinical chemistry and laboratory medicine

authors： Daher R,Beaini M

更新日期：1998-09-01 00:00:00

abstract：:A questionnaire to assess knowledge of the expected elevation in serum K measurement with different grades of hemolysis was administered to medical technologists working in biochemistry laboratories, hospital physicians and nurses. The questions involved different grades of hemolysis (mild, 1.0, moderate, 2.5 and seve...

journal_title：Clinical chemistry and laboratory medicine

authors： Hawkins RC

更新日期：2005-01-01 00:00:00

abstract：Background:Anti-mitochondrial autoantibodies (AMA) detected by indirect immunofluorescence (IIF) on rodent tissues are the diagnostic marker of primary biliary cholangitis (PBC). However, up to 15% of patients with PBC are AMA-negative by IIF. In the effort to close the serological gap and improve the diagnostic sensit...

journal_title：Clinical chemistry and laboratory medicine

authors： Villalta D,Seaman A,Tiongson M,Warren C,Bentow C,Bizzaro N,Alessio MG,Porcelli B,Norman GL,Mahler M

更新日期：2020-04-10 00:00:00

abstract：:Since serum and plasma D-dimer concentrations correlate very well, we evaluated the comparability of other haemostasis activation markers in plasma and serum. Prothrombin fragment F1+2, fibrin monomer and D-dimer concentrations were measured with commercially available immunoassays in serum and plasma. Serum to plasma...

journal_title：Clinical chemistry and laboratory medicine

authors： Korte W,Riesen WF

更新日期：2001-07-01 00:00:00

abstract：BACKGROUND:The differential diagnosis between bacterial and viral meningitis is not easy in some cases. Cerebrospinal fluid (CSF) analysis is essential for establishing this diagnosis. The objectives were to quantitate lactic acid (LA) concentrations in bacterial and viral meningitis, and other central nervous system (...

journal_title：Clinical chemistry and laboratory medicine

authors： de Almeida SM,Faria FL,de Goes Fontes K,Buczenko GM,Berto DB,Raboni SM,Vidal LR,Nogueira MB

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Apolipoprotein E (APOE) is a key player in lipid transport and metabolism and exists in three common isoforms: APOE2, APOE3 and APOE4. The presence of the E4 allelic variant is recognized as a major genetic risk factor for dementia and other chronic (neuro)degenerative diseases. The availability of a validat...

journal_title：Clinical chemistry and laboratory medicine

authors： Badrnya S,Doherty T,Richardson C,McConnell RI,Lamont JV,Veitinger M,FitzGerald SP,Zellner M,Umlauf E

更新日期：2018-04-25 00:00:00

abstract：:We report on the performance of a new test, holotranscobalamin, as compared to well established markers of vitamin B12 deficiency (plasma cobalamins, methylmalonic acid, and homocysteine). Holotranscobalamin was analyzed in 143 samples by a competitive radiobinding assay (Axis-Shield). Employing a cut-off value of 50 ...

journal_title：Clinical chemistry and laboratory medicine

authors： Hvas AM,Nexo E

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:Highly-sensitive cardiac troponin (cTn) assays are being introduced into the market. In this study we argue that the classification of cTn assays into sensitive and highly-sensitive is flawed and recommend a more appropriate way to characterize analytical sensitivity of cTn assays. STUDY:The raw data of 225...

journal_title：Clinical chemistry and laboratory medicine

authors： Ungerer JP,Pretorius CJ

更新日期：2014-04-01 00:00:00

abstract：BACKGROUND:Ebola virus, an enveloped virus, is the cause of the largest and most complex Ebola virus disease (EVD) outbreak in West Africa. Blood or body fluids of an infected person may represent a biohazard to laboratory workers. Laboratory tests of virus containing specimens should be conducted in referral centres a...

journal_title：Clinical chemistry and laboratory medicine

authors： Tempestilli M,Pucci L,Notari S,Di Caro A,Castilletti C,Rivelli MR,Agrati C,Pucillo LP

更新日期：2015-11-01 00:00:00

abstract：:In the relatively short time frame since the introduction of next generation sequencing, it has become a method of choice for complex genomic research studies. As a paradigm shifting technology, we are now witnessing its translation into clinical diagnostic laboratories for patient care. Multi-gene panels for a variet...

journal_title：Clinical chemistry and laboratory medicine

authors： Coonrod EM,Margraf RL,Voelkerding KV

更新日期：2011-12-23 00:00:00

abstract：BACKGROUND:The dogma of establishing intra-laboratory reference limits (RLs) and their periodic review cannot be fulfilled by most laboratories due to the expenses involved. Thus, most laboratories adopt external sources for their RLs, often neglecting the problems of transferability. This is particularly problematic f...

journal_title：Clinical chemistry and laboratory medicine

authors： Arzideh F,Wosniok W,Haeckel R

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND:Although many reports have elucidated pathophysiological characteristics of abnormal bone metabolism in patients with type 2 diabetes mellitus (DT2), determinants of bone mineral density (BMD) in patients with DT2 are still controversial. METHODS:We examined 168 Belarussian men 45-60 years of age. Plasma to...

journal_title：Clinical chemistry and laboratory medicine

authors： Vasilkova O,Mokhort T,Sanec I,Sharshakova T,Hayashida N,Takamura N

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:The discovery of mutations in the epidermal growth factor receptor gene (EGFR) related to the clinical response to tyrosine kinase inhibitors, has transformed the management of non-small cell lung cancer (NSCLC). Several methods have been developed for determination of mutations in EGFR, with different sensi...

journal_title：Clinical chemistry and laboratory medicine

authors： Martínez-Carretero C,Pascual FI,Rus A,Bernardo I

更新日期：2017-10-26 00:00:00

abstract：BACKGROUND:Cholesterol homeostasis disorders may cause dyslipidemia, atherosclerosis progression and coronary artery disease (CAD) development. Evaluation of non-cholesterol sterols (NCSs) as synthesis and absorption markers, and lipoprotein particles quality may indicate the dyslipidemia early development. This study ...

journal_title：Clinical chemistry and laboratory medicine

authors： Gojkovic T,Vladimirov S,Spasojevic-Kalimanovska V,Zeljkovic A,Vekic J,Kalimanovska-Ostric D,Djuricic I,Sobajic S,Jelic-Ivanovic Z

更新日期：2017-03-01 00:00:00

abstract：:We discovered a hitherto undescribed insertion/deletion (I/D) polymorphism of 7 base pairs in intron 4 of the HP1 allele and intron 4 and 6 of the HP2 allele. Genotyping was performed in 311 Belgian subjects. The association between serum haptoglobin (Hp) and lipid concentrations and the Hp I/D genotypes was investiga...

journal_title：Clinical chemistry and laboratory medicine

authors： Wuyts B,Hetet G,Grandchamp B,Delanghe JR

更新日期：2002-05-01 00:00:00

abstract：:Cancers of the respiratory tract (lung and head and neck) share common aetiologies, risk factors and molecular characteristics. Epigenetic reprogramming is one of the hallmarks of cancer and DNA methylation is currently the best-studied form. There are a number of characteristics of DNA methylation, which seem advanta...

journal_title：Clinical chemistry and laboratory medicine

authors： Markopoulou S,Nikolaidis G,Liloglou T

更新日期：2012-10-01 00:00:00

abstract：BACKGROUND:Serum free light chain (sFLC) measurements are increasingly important in the context of screening for monoclonal gammopathies, prognostic stratification, and monitoring of therapy responses. At the same time, analytical limitations have been reported with the currently available nephelometric and turbidimetr...

journal_title：Clinical chemistry and laboratory medicine

authors： Jacobs JFM,de Kat Angelino CM,Brouwers HMLM,Croockewit SA,Joosten I,van der Molen RG

更新日期：2018-01-26 00:00:00

abstract：BACKGROUND:Low-density lipoprotein cholesterol (LDL-C) is an established risk factor for cardiovascular disease and is usually estimated by the Friedewald formula (FF) calculated from three parameters, namely, total cholesterol (TC), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C). We aimed to devel...

journal_title：Clinical chemistry and laboratory medicine

authors： Hu CY,Lee CL,Sheu WH,Wang JS,Lee IT,Song YM,Fu CP,Ling JT,Li YF,Lin SY

更新日期：2015-10-01 00:00:00

abstract：:The pathogenesis of cobalamin (Cbl)-deficient (Cbl-D) neuropathy and the role of normal prions (PrPcs) in myelin maintenance are both subjects of debate. We have demonstrated that Cbl deficiency damages myelin by increasing tumor necrosis factor (TNF)-α, and decreasing epidermal growth factor (EGF) levels in the rat c...

journal_title：Clinical chemistry and laboratory medicine

authors： Scalabrino G,Veber D

更新日期：2013-03-01 00:00:00

abstract：:Thrombotic risk factors and thrombosis in children has been receiving increased attention. True idiopathic thrombosis is extremely rare in children. Most patients have a significant underlying medical condition and the presence of a central catheter is the most important risk factor. Children are more likely than adul...

journal_title：Clinical chemistry and laboratory medicine

authors： Trampus-Bakija A

更新日期：2010-12-01 00:00:00