Abstract:
:Oxidative stress has been implicated in several neurodegenerative diseases affecting both neuronal and glial cells. The aim of this study was to investigate the involvement of reactive oxygen species in glutamate-evoked activation of NF-kappaB in primary astrocytes. A prolonged exposure to glutamate (24 h) caused a depletion of intracellular glutathione that, in astroglial cells, has been considered a biochemical change typical of early astrocyte dysfunction, leading to cell alterations occurring in the gliosis. These effects were initiated by AMPA/KA receptor activation and almost completely blocked by anti-oxidants. Indeed, we provide evidence that the incubation of primary astrocytes with a hydrophilic derivative of tocopherol, such as IRFI 016, was useful to reduce glutamate-induced oxidative effects. This agent also reduced in a dose-dependent manner the nuclear translocation of both p50 and p65 subunits of NF-kappaB. Altogether, these data confirm that GSH content plays a pivotal role to determine oxidative response to glutamate injury in primary astrocyte cultures and that NF-kappaB pathway is involved in this response. Furthermore, the positive effects obtained by IRFI 016 to prevent nuclear translocation of NF-kappaB may suggest new pharmacological strategies for antioxidant therapy and neuroprotection.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Caccamo D,Campisi A,Marini H,Adamo EB,Li Volti G,Squadrito F,Ientile Rdoi
10.1016/j.expneurol.2005.01.014keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
377-83issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(05)00038-5journal_volume
193pub_type
杂志文章abstract::Previous work utilizing the 2-deoxyglucose (2-DG) technique demonstrated that denervation, both direct (enucleation) or indirect (retinal receptor destruction, leaving ganglion cells intact), resulted in a depression followed by an increase in glucose metabolism in the superior colliculus (SC) of the mature hooded rat...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(89)90040-x
更新日期:1989-06-01 00:00:00
abstract::We previously showed that overexpressing the 70-kDa inducible heat shock protein in primary astrocyte cultures and in a rodent stroke model using viral vectors resulted in protection from ischemia and ischemia-like injury. However, viral transfection could potentially provoke a stress response itself; therefore, we ex...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2000.7614
更新日期:2001-07-01 00:00:00
abstract::Axons of the CNS do normally not regenerate after injury, in contrast to axons of the PNS. This is due to a different microenvironment at the site of the lesion as well as a particular intrinsic program of axonal regrowth. Although transplantation of peripheral nerve tissue bridges is perhaps the most successful appro...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7204
更新日期:1999-11-01 00:00:00
abstract::A significant proportion of neurologic patients suffer electroencephalographic (EEG) seizures in the acute phase following traumatic or ischemic brain injury, including many without overt behavioral manifestations. Although such nonconvulsive seizures may exacerbate neuropathological processes, they have received limi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/s0014-4886(02)00013-4
更新日期:2003-02-01 00:00:00
abstract::Human recombinant activated factor-VII (rFVIIa) has been used successfully in the treatment of spontaneous intracerebral hemorrhage. In addition, there is increasing interest in its use to treat uncontrolled bleeding of other origins, including trauma. The aim of this study was to evaluate the safety and potential eff...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2007.12.019
更新日期:2008-04-01 00:00:00
abstract::It is widely accepted that the immune system plays important functional roles in regeneration after injury to the spinal cord. Immune response towards injury involves a complex interplay of immune system cells, such as neutrophils, macrophages and microglia, T- and B-lymphocytes. We investigated the influence of the l...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.07.016
更新日期:2012-10-01 00:00:00
abstract::Infusions of an excitant amino acid, N-methyl-D-aspartate (NMDA) into the inferior colliculus (IC) render normal rats susceptible to audiogenic seizures (AGS) and/or spontaneous audiogenic-like seizures without tonic components. The excess excitant amino acid in the IC and the anticonvulsant effects of NMDA antagonist...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(91)90070-s
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Cognitive impairment in humans with Alzheimer's disease (AD) and in animal models of Aβ-pathology can be ameliorated by treatments with the nuclear receptor peroxisome proliferator-activated receptor-gamma (PPARγ) agonists, such as rosiglitazone (RSG). Previously, we demonstrated that in the Tg2576 animal mo...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.05.005
更新日期:2017-09-01 00:00:00
abstract::The rat model of combined central fluid percussion traumatic brain injury (TBI) and bilateral entorhinal cortical lesion (BEC) produces profound, persistent cognitive deficits, sequelae associated with human TBI. In contrast to percussive TBI alone, this combined injury induces maladaptive hippocampal plasticity. Rece...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2000.7484
更新日期:2000-11-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.10.023
更新日期:2018-02-01 00:00:00
abstract::Photic entrainment of circadian rhythms in mammals is mediated through a direct retinal projection to the core region of the suprachiasmatic nucleus (SCN), the circadian clock. A proportion of this projection contains the low-affinity p75 neurotrophic receptor (p75NTR). Neonatal monosodium glutamate (MSG) treatment, w...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2002.7969
更新日期:2002-08-01 00:00:00
abstract::Spinal axons in the larval sea lamprey regenerate following a complete spinal transection. It is not known whether regenerating growth cones require contact with cellular elements or whether the basement membrane and collagenous meninx primitiva which surround the spinal cord are sufficient for neurite out-growth. To ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(91)90114-r
更新日期:1991-04-01 00:00:00
abstract::While past neuroimaging methods have contributed greatly to our understanding of brain function after traumatic brain injury (TBI), resting state functional MRI (rsfMRI) connectivity methods have more recently provided a far more unbiased approach with which to monitor brain circuitry compared to task-based approaches...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.12.020
更新日期:2016-03-01 00:00:00
abstract::This study examines cell death and proliferation in the white matter after neonatal stroke. In postnatal day 7 injured rat, there was a marked reduction in myelin basic protein (MBP) immunostaining mainly corresponding to numerous pyknotic immature oligodendrocytes and TUNEL-positive astrocytes in the ipsilateral exte...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.01.037
更新日期:2006-05-01 00:00:00
abstract::The tendency of a rat to approach the wall of an open-field and to travel along the perimeter of the field (termed as "peritaxis" or "wall-facing") is affected by unilateral removal of the vibrissae. Peritaxis is lateralized by hemivibrissotomy. The finding that the dopamine agonist apomorphine reversed the direction ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90172-0
更新日期:1988-03-01 00:00:00
abstract::The effect of olfactory deprivation in the postnatal development of the anterior olfactory nucleus (AON) was studied in 60-day-old rats which underwent unilateral naris closure after birth (postnatal day 1). Volumetric and morphometric analyses of the AON ipsilateral and contralateral to the closed naris were performe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7760
更新日期:2001-10-01 00:00:00
abstract::Aberrant accumulation of neurofilaments is a feature of human motor neuron diseases. Experimentally motor neuron disease can be induced in transgenic mice by overexpressing the mouse neurofilament light subunit (NF-L), the human heavy subunit (NF-H), or mouse peripherin. Here we describe that mice harboring a bacteria...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/s0014-4886(03)00206-1
更新日期:2003-11-01 00:00:00
abstract::Implantable silicon microelectrode array technology is a useful technique for obtaining high-density, high-spatial resolution sampling of neuronal activity within the brain and holds promise for a wide range of neuroprosthetic applications. One of the limitations of the current technology is inconsistent performance i...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.04.020
更新日期:2005-09-01 00:00:00
abstract::The anticonvulsant long-term efficacy of diazepam was studied in amygdala-kindled rats. The drug was administered three times daily at doses of 5 mg/kg i.p. for 2 weeks in fully kindled animals. The severity of the kindled seizures was markedly reduced throughout the period of treatment, while tolerance developed to t...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90026-3
更新日期:1985-11-01 00:00:00
abstract::The present study was performed to establish an anatomical context for studies of phrenic motor function in mouse models of central nervous system trauma and disease. Application of cholera toxin β-subunit to the diaphragm of adult C57BL/6 mice revealed a columnar organization of phrenic motoneurons (PhMNs) which exte...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2010.08.026
更新日期:2010-11-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.02.134
更新日期:2006-08-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113320
更新日期:2020-08-01 00:00:00
abstract::Overexpression of nerve growth factor (NGF) using adenoviruses (Adts) after spinal cord injury induces extensive regeneration and sprouting of calcitonin-gene-related peptide immunoreactive (CGRP-IR) fibers, whereas overexpression of cell adhesion molecules (CAMs) has no effect on the normal distribution of these fibe...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.06.001
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:The pathogenesis of pain in irritable bowel syndrome (IBS) is poorly understood and treatment remains difficult. We have previously reported that TTX-resistant (TTX-R) sodium channels in colon-specific dorsal root ganglion (DRG) neurons were sensitized and the expression of the endogenous hydrogen sulfide pr...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2013.06.027
更新日期:2013-10-01 00:00:00
abstract::Exposing rat dorsal root ganglion (DRG) neurons to dibutyryl cAMP (db-cAMP) enables central branches to regenerate in the spinal cord by nullifying the ability of CNS myelin to inhibit elongation. A conditioning lesion (CL) promotes similar regeneration of central branches in the spinal cord by increasing neuronal cAM...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.03.010
更新日期:2004-10-01 00:00:00
abstract::The pathophysiology of the tics that define Gilles de la Tourette syndrome (TS) is not well understood. Local disinhibition within the striatum has been hypothesized to play a pathogenic role. In support of this, experimental disinhibition by local antagonism of GABA-A receptors within the striatum produces tic-like p...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.01.001
更新日期:2015-03-01 00:00:00
abstract::Systemic administration of kainic acid (12 mg/kg, i.p.), causing the established epileptic syndrome and the associated specific pattern of limbic nerve cell loss, led to substantial time-dependent increases in the brain metabolism of the endogenous excitotoxin, quinolinic acid. These changes preferentially affected bi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90140-9
更新日期:1988-01-01 00:00:00
abstract::Adult cats were subjected to subarachnoid hemorrhage by three repeated intracisternal injections of autogenous nonheparinized blood (2 ml/injection) at weekly intervals. Histological studies (light and electron microscope) were made of the basilar artery and the middle cerebral arteries of all animals. In the subarach...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90103-2
更新日期:1986-11-01 00:00:00
abstract::In this study we have characterised the locomotor recovery, and temporal profile of cell loss, in a novel thoracic compression spinal cord injury (SCI) in the mouse. We have also shown that treatment with docosahexaenoic acid (DHA) is neuroprotective in this model of SCI, strengthening the growing literature demonstra...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.09.015
更新日期:2013-01-01 00:00:00
abstract::An important issue in stem cell biology relates to mechanisms of cellular plasticity. Specifically, could any observed multipotency of, e.g., adult stem cells arise from true transdifferentiation or as a result of cell-cell fusion? We studied this issue using a culture paradigm of astrocyte monolayers and multipotent ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.11.016
更新日期:2006-03-01 00:00:00