Abstract：
:We previously showed that overexpressing the 70-kDa inducible heat shock protein in primary astrocyte cultures and in a rodent stroke model using viral vectors resulted in protection from ischemia and ischemia-like injury. However, viral transfection could potentially provoke a stress response itself; therefore, we examined whether transgenic mice constitutively expressing human heat shock protein 70 were protected from ischemic insults. Astrocyte cultures from brains of heat shock protein 70 transgenic mice were resistant to hydrogen peroxide injury in a dose-dependent fashion, but were less resistant to hypoglycemia and oxygen-glucose deprivation. Because hydrogen peroxide exposure and glucose deprivation are partially dependent on glutathione levels, we determined whether heat shock protein 70 transgenic cultures had altered glutathione levels under normal growth conditions. However, there was no significant difference in glutathione levels between heat shock protein 70 transgenic and wildtype astrocytes. Hippocampal, but not cortical neuron cultures from these same transgenic mice were also protected against oxygen-glucose deprivation and glutamate toxicity. In an in vivo model of permanent focal cerebral ischemia, there was no significant difference in infarct size assessed 24 h postinsult. These results suggest that heat shock protein 70 protects against some but not all kinds of central nervous system injury. The protective effects may be related to the nature and severity of the insults, as well as subpopulations of brain cells and dose-dependent effects of HSP70 overexpression.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Lee JE,Yenari MA,Sun GH,Xu L,Emond MR,Cheng D,Steinberg GK,Giffard RGdoi
10.1006/exnr.2000.7614keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
129-39issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(00)97614-3journal_volume
170pub_type
杂志文章abstract：:Alzheimer's disease is associated with an age-related accumulation of Abeta and inflammation. The inflammatory mediator, TNFalpha activates a signaling cascade involving NFkappaB translocation to the nucleus and a beneficial or detrimental transcriptional response, depending on the age of the neurons and the type of s...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.05.007
更新日期：2008-09-01 00:00:00
abstract：:Fish optic nerve sections were recently shown to be nonpermissive to growth of adult retinal axons. In addition, fish optic nerve myelin was found to inhibit growth of adult retinal axons and this inhibition was neutralized by IN-1 antibodies (known to block rat myelin-associated inhibitors). In this study we examined...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1221
更新日期：1994-12-01 00:00:00
abstract：:Ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) have been shown both in vitro and in vivo to support the survival and differentiation of developing chick or rat motoneurons. To explore the potential use of these and other neurotrophic factors as therapeutic agents in human motoneuron di...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1198
更新日期：1994-12-01 00:00:00
abstract：:The present experiments focused on the morphological interaction between serotonergic (5-HT) and noradrenergic (NA) axons during regeneration following partial axonal denervation in the cerebral cortex in adult rats. The denervation paradigm used employed two neurotoxins, one for 5-HT and one for NA axons, infused tog...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(03)00221-8
更新日期：2003-11-01 00:00:00
abstract：:Rat axial muscle previously has not been studied histochemically. We were interested in determining the fiber composition and fiber distribution in rat lateral longissimus (LL), the large epaxial dorsiflexor muscle active during sexual posturing in the female rat and to determine if estrogen replacement in ovariectomi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90229-7
更新日期：1983-02-01 00:00:00
abstract：:Electrical stimulation of the trigeminal ganglion has been widely used as a model of nociception, characterizing migraine. This treatment is known to evoke release of neuropeptides and neurotransmitters from nerve fibers of the dura mater. On the basis of immunocytochemical investigations, we found that under normal c...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2003.12.001
更新日期：2004-04-01 00:00:00
abstract：:In the embryonic brain, the transcription factor Nkx2.1 is localized in the medial ganglionic eminence and the ventromedial part of the hypothalamus. In the present study, we examined the development of mesencephalic dopamine (DA) neuron system in mice lacking Nkx2.1. In normal mice, tyrosine hydroxylase-immunoreactiv...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(03)00030-x
更新日期：2003-07-01 00:00:00
abstract：:Cerebrospinal fluid (CSF) is produced by the choroid plexus and moved by multi-ciliated ependymal cells through the ventricular system of the vertebrate brain. Defects in the ependymal layer functionality are a common cause of hydrocephalus. N-WASP (Neural-Wiskott Aldrich Syndrome Protein) is a brain-enriched regulato...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.01.011
更新日期：2014-04-01 00:00:00
abstract：:Effects of accumulation of phosphocreatine (P-creatine) on utilization and restoration of high-energy phosphates during anoxia and recovery were studied using thin hippocampal slices from the guinea pig. Incubation of slices with creatine (5 to 30 mM) increased the content of tissue P-creatine, in a linear fashion, an...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90256-x
更新日期：1983-10-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6667
更新日期：1997-11-01 00:00:00
abstract：:Blood flow in the feline thoracic spinal cord was measured, using a laser-Doppler flowmetry. Rhythmic changes in the spinal cord blood flow were repeatedly observed with a frequency of about three to eight cycles per minute, unrelated to the respiratory or cardiac cycles. These changes were unaffected by hypertension ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90009-x
更新日期：1988-08-01 00:00:00
abstract：:An autoradiographic method is described for detecting changes in ornithine decarboxylase in axotomized superior cervical ganglion neurons of rats using [3H]difluoromethylornithine. An increase in binding to neurons was seen at 12 h and 1 day after crushing the postganglionic nerves. Binding returned to control values ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(86)90097-x
更新日期：1986-05-01 00:00:00
abstract：:The presence of the alpha2macroglobulin receptor/low density lipoprotein receptor-related protein (alpha2Mr/LRP) and its ligands alpha2macroglobulin (alpha2M), apoliprotein E, and plasminogen activators was detected in senile plaques of Alzheimer's disease (AD). To explore a possible role of alpha2M in neurodegenerati...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1998.6978
更新日期：1999-02-01 00:00:00
abstract：:The tachykinin neuropeptides acting at NK3 receptors affect mean arterial pressure (MAP) through both neuroendocrine and neural mechanisms. NK3 receptors are found in brainstem nuclei that mediate the baroreflex, but the effects of NK3 receptor stimulation on baroreflex function are unknown. The present study tests th...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.12.011
更新日期：2005-05-01 00:00:00
abstract：:The beta-Amyloid peptide (A beta) is hypothesized to mediate the neurodegeneration seen in Alzheimer's disease. Recently, we proposed a new hypothesis to explain the toxicity of A beta based on the free-radical generating capacity of A beta. We have recently demonstrated using electron paramagnetic resonance (EPR) spe...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(95)90041-1
更新日期：1995-02-01 00:00:00
abstract：:Unit activity related to a go/no-go visual discrimination task was studied in four rhesus monkeys. We recorded 272 task-related cells from frontal cortex in a region extending from the midprincipal sulcus to the central sulcus, and medially to the cingulate sulcus. Units located in anterior regions (dorsolateral prefr...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(87)90213-5
更新日期：1987-06-01 00:00:00
abstract：:The age-dependent effects of sympathetic superior cervical ganglionectomy on smooth muscle alpha-adrenoceptor contractile responses were investigated in Müller's muscle in the superior eyelid of the Sprague-Dawley rat. Ganglionectomies in juvenile (32 days) and adult (70 days) rats produced sustained sympathetic dener...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(86)90031-2
更新日期：1986-01-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease characterized by motor neuron death. Since most ALS patients succumb to ventilatory failure from loss of respiratory motor neurons, any effective ALS treatment must preserve and/or restore breathing capacity. In rats over-expressi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.08.011
更新日期：2015-11-01 00:00:00
abstract：:Neurogenesis continues in various regions of the central nervous system (CNS) throughout life. As the mitogen basic fibroblast growth factor (bFGF) can proliferate neuronal precursors of CNS neurons in culture, and is also upregulated within adult dorsal root ganglia following axotomy, it is possible that the postnata...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2001.7761
更新日期：2001-11-01 00:00:00
abstract：:Chondroitin sulfate proteoglycans (CSPGs), extracellular matrix molecules that increase dramatically following a variety of CNS injuries or diseases, have long been known for their potent capacity to curtail cell migrations as well as axon regeneration and sprouting. The inhibition can be conferred through binding to ...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2020.113276
更新日期：2020-06-01 00:00:00
abstract：:In what ways do passive touch and active touch require particular properties of cortical physiology? Because it is not easy to make predictions "bottom up" from neurophysiology and anatomy to behavior, there have been surprises in studying behavioral deficits following selective ablations of SI versus SII. For example...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1006/exnr.1994.1017
更新日期：1994-01-01 00:00:00
abstract：:The precise mechanisms underlying the efficacy of intravenous immunoglobulin (IVIg) in autoimmune neurological disorders including Guillain-Barré syndrome (GBS) are not known. Anti-ganglioside antibodies have been reported to be pathogenic in some variants of GBS, and we have developed passive transfer animal models t...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2016.05.020
更新日期：2016-08-01 00:00:00
abstract：:Previous studies have demonstrated that neonatal cultures of astrocytes express functional endothelin (ET) receptors. To determine if similar ET receptors are expressed by adult glia we used 125I-ET-1 to examine the expression of ET receptors both in vivo in the normal and transected optic nerves of the rabbit and rat...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6468
更新日期：1997-05-01 00:00:00
abstract：:The contribution of chondroitin sulfate proteoglycan (CSPG) in the suppression of axonal growth in rat spinal cord has been examined by means of an in vitro bioassay in which regenerating neurons are grown on tissue section substrata. Dissociated embryonic chick dorsal root ganglionic neurons were grown on normal and ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1998.6951
更新日期：1998-12-01 00:00:00
abstract：:MicroRNAs (miRs) are powerful regulators of CNS development and diseases. Plasma and cerebrospinal fluid (CSF) miRs have recently been implicated as potential new sources for biomarker development. Previously we showed that miR-124-3p, an essential miR for neuronal identity, is highly abundant in neuronal exosomes and...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2020.113414
更新日期：2020-11-01 00:00:00
abstract：:In previous studies of our group, we have reported differential alterations in opioidergic receptor subtypes densities in infarcted and periinfarcted brain tissue following middle cerebral artery occlusion (MCAO) in mice. Other studies have also described subcortical alterations consecutive to focal cortical ischemia....
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2000.7400
更新日期：2000-08-01 00:00:00
abstract：:Mitochondria play a pivotal role in the cascade of events associated with cell death pathways that are involved with several forms of neurodegeneration. Recent findings show that in the Bax/Bak-dependent pathway of apoptosis, the release of cytochrome c from mitochondria is a consequence of two carefully coordinated e...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2009.05.021
更新日期：2009-08-01 00:00:00
abstract：:The axonal trajectories of inspiratory bulbospinal neurons were examined after deposition of the anterograde neuronal tracer phaseolus vulgaris leucoagglutinin (PHA-L) into the rostral ventral respiratory group in rats. At the level of the phrenic nucleus, PHA-L-labeled bulbospinal axons crossed the midline of the spi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(91)90061-g
更新日期：1991-01-01 00:00:00
abstract：:Given the prevalence and societal impact of autism spectrum disorders (ASD), there is an urgent need to develop innovative preventative strategies and treatments to reduce the alarming number of cases and improve core symptoms for afflicted individuals. Translational efforts between clinical and preclinical research a...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2017.07.021
更新日期：2018-01-01 00:00:00
abstract：:Posttraumatic inflammation has been implicated in secondary tissue damage after spinal cord injury (SCI). Tumor necrosis factor-alpha (TNF-alpha) is a key inflammatory mediator that is increasingly expressed after SCI. The effect of TNF-alpha is mediated through its receptors TNFR1 (p55) and TNFR2 (p75). However, whet...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(03)00135-3
更新日期：2003-10-01 00:00:00
