Abstract:
:In this study we have characterised the locomotor recovery, and temporal profile of cell loss, in a novel thoracic compression spinal cord injury (SCI) in the mouse. We have also shown that treatment with docosahexaenoic acid (DHA) is neuroprotective in this model of SCI, strengthening the growing literature demonstrating that omega-3 polyunsaturated fatty acids are neuroprotective after SCI. Compression SCI in C57BL/6 mice was produced by placing a 10 g weight for 5 min onto a 2 mm × 1.5 mm platform applied to the dura at vertebral level T12. Mice partly recovered from complete hindlimb paralysis and by 28 days post-surgery had plateaued at an average BMS locomotor score of 4.2, equivalent to weight support with plantar stepping. During the same period, neuronal loss at the epicentre increased from 26% of ventral horn neurons by day 1, to 68% by day 28. Delayed loss of oligodendrocytes was also seen (e.g. 84% by day 28 in the dorsal columns) and microglia/macrophage activation was maximal at 7 days. In contrast, axonal damage, judged by a decrease in the non-phosphorylated form of 200 kD neurofilament, was an early event, as the loss was seen by day 1 and did not change markedly over time. Mice that received an intravenous (i.v.) injection of 500 nmol/kg DHA 30 min after SCI, showed improved locomotor recovery and, at 28 day survival, reduced neuronal, oligodendrocyte and neurofilament loss, and reduced microglia/macrophage activation. For some of these indices of SCI, enrichment of the diet with 400 mg/kg/day DHA led to further improvement. However, dietary DHA supplementation, without the initial i.v. injection, was ineffective.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Lim SN,Huang W,Hall JC,Michael-Titus AT,Priestley JVdoi
10.1016/j.expneurol.2012.09.015subject
Has Abstractpub_date
2013-01-01 00:00:00pages
13-27eissn
0014-4886issn
1090-2430pii
S0014-4886(12)00377-9journal_volume
239pub_type
杂志文章abstract::One approach to repair of transected nerves is to attempt extrinsic guidance of axons across the gaps. We inserted the proximal and distal stumps of severed mouse sciatic nerves into opposite ends of biodegradable polyester tubes. The nerves and ensheathing tubes were examined after postoperative survival times of as ...
journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2013.03.002
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章,评审
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90200-6
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7734
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doi:10.1016/j.expneurol.2016.06.014
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.07.010
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journal_title:Experimental neurology
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doi:10.1016/0014-4886(87)90314-1
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1050
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1993.1041
更新日期:1993-03-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章,评审
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更新日期:2012-11-01 00:00:00
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
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pub_type: 杂志文章,评审
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