Abstract:
:The developing retina is characterized by 'retinal waves', spontaneous depolarizations that propagate through a developing network of interneurons and retinal ganglion cells. Although the circuitry underlying retinal waves is well characterized, the secreted factors that are critical for its normal development are not defined. Dissociated cell culture provides an ideal system for defining these factors; however, it is difficult to recapitulate retinal circuitry in culture. Here we demonstrate that by culturing dissociated retinal neurons in the presence of cells from the superior colliculus (SC), retinal neurons form networks that are similar to those described in the intact retina. Whole-cell voltage clamp recordings reveal the presence of a spontaneously active network of interneurons. In addition, we observed spontaneous, propagating activity reminiscent of that observed in the intact retina. We propose that the presence of factors secreted from the SC results in the development of networks that reproduce critical features of the intact retina.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Colicos MA,Firth SI,Bosze J,Goldstein J,Feller MBdoi
10.1159/000082283keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
406-16issue
5-6eissn
0378-5866issn
1421-9859pii
82283journal_volume
26pub_type
杂志文章abstract::Developmental expression of neutral monoglycosylceramides (MGCs) has been examined in rat brain from embryonic day 15 (E15) to postnatal day 30 (P30) and adulthood. To this point, glucosylceramide (GlcCer) is the only MGC that has been characterized in embryonic brain. Galactosylceramide (GalCer) appears at P1, increa...
journal_title:Developmental neuroscience
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doi:10.1159/000112154
更新日期:1991-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1159/000329420
更新日期:2011-01-01 00:00:00
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journal_title:Developmental neuroscience
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doi:10.1159/000091921
更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1159/000111893
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doi:10.1159/000497273
更新日期:2019-03-28 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112200
更新日期:1991-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111346
更新日期:1993-01-01 00:00:00
abstract::Perinatal hypoxic ischemic (HI) brain injury is a leading cause of long-term neurological handicap in newborn babies. Recently, excessive activity of matrix metalloproteinases (MMPs), and in particular MMP-9, has been implicated in the aetiology of HI injuries to the immature brain. Our previous study suggested that M...
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pub_type: 杂志文章
doi:10.1159/000343257
更新日期:2012-01-01 00:00:00
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