Abstract：
:T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants in cereals. Human lymphoid cell lines of T (MOLT-4) or B (IM-9) lineage were used to characterize the cytotoxic effects mediated by T-2 at different concentrations (0.1 pg/ml to 1 microg/ml). After 24 h, membrane damage was observed by Trypan blue dye exclusion in IM-9 cells with a 50% cytotoxic concentration (CC50) of 0.2 ng/ml, whereas CC50 for MOLT-4 cells was 0.6 microg/ml (gmicro). At a T-2 concentration of 0.01 microg/ml, apoptosis was seen in MOLT-4 cells by Annexin V binding as early as after 4 h. T-2 toxin determined sustained (48 h) immunosuppression on both cell lines, as evaluated by BrdU and MTT assays. Cytotoxicity appeared to be due to early apoptosis in MOLT-4 cells, as indicated by increased Annexin V binding and activation of caspase-3, and to direct cell membrane damage in IM-9 cells.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Minervini F,Fornelli F,Lucivero G,Romano C,Visconti Adoi
10.1016/j.tox.2005.01.007keywords:
subject
Has Abstractpub_date
2005-05-15 00:00:00pages
81-91issue
1eissn
0300-483Xissn
1879-3185pii
S0300-483X(05)00032-6journal_volume
210pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:Exposure to polycyclic aromatic hydrocarbons (PAHs) always involves complex mixtures that may induce synergistic or antagonistic effects on the genotoxic properties and make risk assessment more difficult. In this study, we evaluated how particulate PAHs modulated the formation of DNA damage induced by carcinogenic be...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2010.09.002
更新日期：2011-01-11 00:00:00
abstract：:Inorganic arsenic (iAs), a ubiquitous element and a natural drinking water contaminant, has been found to impair male reproductive function. However, the effect of long-term exposure to arsenic on testis damage and its underlying mechanisms still require further evaluation. In the study, male C57BL/6 mice (4 weeks) we...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2018.09.010
更新日期：2019-01-01 00:00:00
abstract：:The organophosphorus nerve agent soman is an irreversible cholinesterase (ChE) inhibitor that can produce long-lasting seizures and seizure-related brain damage (SRBD) in which acetylcholine and glutamate are involved. Since these neurotransmitters play a key-role in the auditory function, it was hypothesized that a h...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2010.08.014
更新日期：2010-11-09 00:00:00
abstract：:NLRC5, a newly found member of the NLR family and the largest member of nucleotide-binding, has been reported to regulate immune responses and is associated with hepatocellular carcinoma (HCC). We investigated the mechanisms and signaling pathways of NLRC5 in HCC progression. Increased expression of NLRC5, vascular en...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2016.06.012
更新日期：2016-06-01 00:00:00
abstract：:There is a close relationship between the central nervous system activity and bone metabolism. Therefore, methamphetamine (METH), which stimulates the central nervous system, is expected to affect bone turnover. The aim of this study was to investigate the role of METH in bone metabolism. Mice were divided into 3 grou...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.01.014
更新日期：2014-05-07 00:00:00
abstract：:An embryotoxicity study on Ponceau 4 R was carried out in SPF Wistar rats. The substance was given by gavage from day 1 to 20 of the gestation period in doses of 0, 1000, 2000, and 4000 mg/kg bw per day, dissolved in distilled water. The foetuses were removed on day 21. No effect due to the treatment with the dye was ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(75)90117-1
更新日期：1975-11-01 00:00:00
abstract：:The reproductive toxicity of vanadium was studied in mice. Male Swiss mice were exposed to sodium metavanadate at doses of 0, 20, 40, 60, and 80 mg/kg per day given in the drinking water for 64 days. To evaluate the fertility of the vanadium-treated animals, males were mated with untreated females for 4 days. A signif...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(93)90181-q
更新日期：1993-06-11 00:00:00
abstract：:Inorganic arsenic (iAs) and its metabolites are transferred to the foetus through the placental barrier and this exposure can compromise the normal development of the unborn. For this reason, we assessed the toxicity of sodium arsenite (iAs(III)) and its metabolites dimethylarsinic acid (DMA(V)), monomethylarsonic aci...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2008.04.008
更新日期：2008-07-30 00:00:00
abstract：:Immunohistochemical methods have been widely used to determine the histogenesis of spontaneous and chemically-induced mouse lung tumors. Typically, antigens for either alveolar Type II cells or bronchiolar epithelial Clara cells are studied. In the present work, the morphological and immunohistochemical phenotype of a...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00060-x
更新日期：2003-05-03 00:00:00
abstract：:Young adult male and female Wistar rats were inhalationally exposed head-only for 1 or 4 h to different anticholinesterase aerosols. The compounds tested were dichlorvos, fenamiphos, methamidophos, parathion, a pyrimidine thiophosphate and the carbamate propoxur. These compounds are direct or indirect inhibitors of ch...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(87)90126-0
更新日期：1987-10-30 00:00:00
abstract：:Several dicationic amphiphilic compounds, such as the immunomodulator tilorone and analogues, impair the lysosomal catabolism of sulphated glycosaminoglycans (GAGs). Thereby they cause lysosomal GAG storage in rats and in cultured fibroblasts of several species including man. The GAG storage is rather slowly reversibl...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(98)00052-3
更新日期：1998-07-03 00:00:00
abstract：:Antidepressants are generally used for treatment of various mood and anxiety disorders. Several studies have shown the anti-tumor and cytotoxic activities of some antidepressants, but the underlying mechanisms were unclear. Maprotiline is a tetracyclic antidepressant and possesses a highly selective norepinephrine reu...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.11.013
更新日期：2013-02-08 00:00:00
abstract：:As vaccines are undoubtedly classified as pharmaceuticals, they have to be submitted to strict non-clinical safety evaluation. The context of their prophylactic use requires that every effort is made to ensure their safe use. Their safety evaluation is complex as they act through a multistage mechanism in which the va...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(02)00055-0
更新日期：2002-05-15 00:00:00
abstract：:Injection of insulin into mice and rabbit has been reported to convert the latent form of phosphoglucomutase (E X Zn) to an active form (E X Mg) without any net increase in the protein associated with this enzyme (Hashimoto et al., Biochem. Biophys. Res. Commun., 27 (1967) 369 and Peck et al., J. Biol. Chem. 242 (1971...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(85)90050-2
更新日期：1985-08-01 00:00:00
abstract：:The prevalence of asthma is increasing worldwide. The increase has been found for both sexes, for all races and age groups. The factors responsible are unclear. The short period of increase makes it unlikely that a genetic change is responsible but rather suggests that either air pollutants or a behavioral change may ...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(02)00298-6
更新日期：2002-12-27 00:00:00
abstract：:Relative cytotoxicity and mechanism of action of thiophenes were investigated in suspensions of freshly isolated renal proximal tubular (PT) and distal tubular (DT) cells using 4-(2-thienyl)butyric acid (TByA) as a model compound. TByA produced significantly greater time- and concentration-dependent cytotoxicity, as a...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(95)03137-5
更新日期：1995-12-10 00:00:00
abstract：:The nature of cancer initiation by fumonisin B(1) (FB(1)) was investigated in rat liver by monitoring the effect of phenobarbital (PB) as cancer promoter and evaluating the involvement of spontaneously initiated cells. A PB promoting regimen (0.05% in the diet) stimulated the outgrowth of FB(1)-induced placental gluta...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2008.06.004
更新日期：2008-09-04 00:00:00
abstract：:Clostridium difficile toxins A and B (TcdA and TcdB) belong to the class of large clostridial cytotoxins and inactivate by glucosylation some low molecular mass GTPases of the Rho-family (predominantly Rho, Rac and Cdc42), known as regulators of the actin cytoskeleton. TcdA and B also represent the main virulence fact...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.12.006
更新日期：2015-02-03 00:00:00
abstract：:The effects of D- and L-T3 were compared in male SK & F Wistar rats to define overall effects on the 'pituitary-thyroid-liver axis' at high doses. After in vivo administration of L-T3 (up to 1 mg/kg orally, or up to 0.1 mg/kg subcutaneously) serum TSH and T4 were decreased in a dose-related manner. Similarly, followin...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(88)90108-4
更新日期：1988-03-01 00:00:00
abstract：:The effect of mangiferin, a naturally occurring xanthone glucoside on cyclophosphamide-induced immunotoxicity and its mode of action in the immune system were investigated. To induce immunotoxicity, adult male Wistar rats were injected weekly with cyclophosphamide intraperitoneally at 100 mg/kg bodyweight. Mangiferin ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2005.06.008
更新日期：2005-11-05 00:00:00
abstract：:Bromofenofos (BF) was administered by gavage once to non-pregnant and pregnant rats at 50 mg/kg and the plasma concentration-time curves of BF and its metabolite dephosphate bromofenofos (DBF) were determined by high-performance liquid chromatography. DBF reached a peak at 9 h, with 113.4 +/- 5.2 micrograms/ml, follow...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(89)90019-x
更新日期：1989-05-15 00:00:00
abstract：:Chlorpyrifos (CPF) has been widely used around the world as a pesticide for both agricultural and residential application. Although various studies have reported toxicity and health-related effects from CPF exposure, the molecular mechanism of CPF toxicity to skin has not been well-characterized. The present study det...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2015.09.006
更新日期：2015-12-02 00:00:00
abstract：:(1) Following the administration of the highly toxic agent 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to pregnant mice, exceedingly low concentrations of this substance were found in the embryo and fetus between gestational days 11 and 18 (between 0.04 and 0.14% of the maternal dose/g tissue). (2) Tissue levels of tCD...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(81)90037-8
更新日期：1981-01-01 00:00:00
abstract：:Cadmium, an environmental neurotoxic compound, produces cognitive disorders, although the mechanism remains unknown. Previously, we described that cadmium induces a more pronounced cell death on cholinergic neurons from basal forebrain (BF). This effect, partially mediated by M1 receptor blockade, triggering it throug...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2017.12.006
更新日期：2018-02-01 00:00:00
abstract：:The present study was conducted in order to study the effect of the PCB congener 3,3', 4,4'-tetrachlorobiphenyl (CB-77) on fetal thyroxin homeostasis in the mouse, and to examine a possible underlying mechanism behind the effect. C57BL mice were treated with 14C-labelled or unlabelled CB-77 (1 or 10 mg/kg body wt.) on...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(95)03169-g
更新日期：1996-01-08 00:00:00
abstract：:Deltamethrin is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control, protection of foodstuff and disease vector control. The objective of this study was to investigate the propensity of deltamethrin to induce oxidative stress and changes in biochemical parameters and enzyme activities i...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2006.08.008
更新日期：2006-10-29 00:00:00
abstract：:A major goal in modern pharmacotechnology is to increase the therapeutic index of drugs by using nanoparticulate vehicle systems in order to ensure slow release or targeted delivery of drugs. With all great benefits, however, these innovative therapies can carry a risk for acute immune toxicity manifested in hypersens...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2005.07.023
更新日期：2005-12-15 00:00:00
abstract：:The activity of the haem biosynthetic enzymes delta-aminolaevulinic acid synthetase (ALA.S) and delta-aminolaevulinic acid dehydratase (ALA.D) were measured in the peripheral blood of a group of lead workers and control subjects. The haem precursor delta-aminolaevulinic acid (ALA) was measured in blood and urine, whil...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(78)90026-4
更新日期：1978-02-01 00:00:00
abstract：:Paracetamol, in toxic doses, is associated with extensive liver damage. This represents one of the common causes of morbidity and mortality in drug poisoning cases. This study was undertaken to investigate the possible potentiation of the hepatoprotective action of N-acetylcysteine (NAC) by cimetidine (CMD), an inhibi...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00069-3
更新日期：1997-09-05 00:00:00
abstract：:In the present study, we demonstrated the cytotoxic effect of a dimethylarsenic compound in seaweed, (R)-(2',3'-dihydroxypropyl) 5- deoxy-5-dimethylarsinoyl-beta-D-riboside, namely arsenosugar (AsSug), on mammalian cells, murine macrophages, in comparison with that of an inorganic arsenical, arsenite, in vitro. More t...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(97)00101-7
更新日期：1997-10-19 00:00:00
